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The map label for this gene is cspLA [H]

Identifier: 150395218

GI number: 150395218

Start: 2898311

End: 2898511

Strand: Direct

Name: cspLA [H]

Synonym: SaurJH1_2784

Alternate gene names: 150395218

Gene position: 2898311-2898511 (Clockwise)

Preceding gene: 150395217

Following gene: NA

Centisome position: 99.72

GC content: 38.31

Gene sequence:

>201_bases
ATGAATAACGGTACAGTAAAATGGTTTAACGCAGAAAAAGGTTTTGGTTTCATCGAACAAGAAAATGGCGGAGACGTATT
CGTACATTTCTCAGGTATCGCTAGCGATGGCTACAAAACTTTAGAAGAAGGTCAAAAAGTTACTTTCGAAATCACTGAAG
GTCAACGTGGAGACCAAGCAGTTAACGTACAAACTGTTTAA

Upstream 100 bases:

>100_bases
CTCGTGAATTAAATTGTAGTGTATTTGTTTAGAATATCCTCTTTTTTAGTTATGAATTTGTTACAAATATTAAGTGCAAA
AGCACATGGAGGTTTTCTAT

Downstream 100 bases:

>100_bases
TCTTACAAAATAAAACGACTCATTATAAATGAATCGCTTTGATTACCAACAAGGTTCTAAATCTTGTTGGTATTTTTTTA
TGCTATTTTGGATAATATGA

Product: cold-shock protein DNA-binding

Products: NA

Alternate protein names: CspL [H]

Number of amino acids: Translated: 66; Mature: 66

Protein sequence:

>66_residues
MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEGQKVTFEITEGQRGDQAVNVQTV

Sequences:

>Translated_66_residues
MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEGQKVTFEITEGQRGDQAVNVQTV
>Mature_66_residues
MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEGQKVTFEITEGQRGDQAVNVQTV

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=64, Percent_Identity=60.9375, Blast_Score=81, Evalue=1e-17,
Organism=Escherichia coli, GI1786841, Length=61, Percent_Identity=63.9344262295082, Blast_Score=79, Evalue=5e-17,
Organism=Escherichia coli, GI1787839, Length=61, Percent_Identity=63.9344262295082, Blast_Score=79, Evalue=7e-17,
Organism=Escherichia coli, GI2367114, Length=64, Percent_Identity=59.375, Blast_Score=78, Evalue=1e-16,
Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=60.9375, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1787107, Length=67, Percent_Identity=52.2388059701493, Blast_Score=73, Evalue=4e-15,
Organism=Escherichia coli, GI1787834, Length=61, Percent_Identity=59.0163934426229, Blast_Score=70, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7258; Mature: 7258

Theoretical pI: Translated: 4.25; Mature: 4.25

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEGQKVTFEITEGQRGDQA
CCCCEEEEEECCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCEE
VNVQTV
EEEEEC
>Mature Secondary Structure
MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEGQKVTFEITEGQRGDQA
CCCCEEEEEECCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCEE
VNVQTV
EEEEEC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669; 9439003 [H]