BacMap

Definition	Staphylococcus aureus subsp. aureus JH1, complete genome.
Accession	NC_009632
Length	2,906,507

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The map label for this gene is yiaE [C]

Identifier: 150394808

GI number: 150394808

Start: 2457849

End: 2458802

Strand: Direct

Name: yiaE [C]

Synonym: SaurJH1_2371

Alternate gene names: 150394808

Gene position: 2457849-2458802 (Clockwise)

Preceding gene: 150394807

Following gene: 150394816

Centisome position: 84.56

GC content: 35.64

Gene sequence:

>954_bases
ATGGAAAAAGTTTATGTTGCTGGTGCAATACCAGAAGTAGGGTTAAAACTTTTACAAGAACATTTTGAAGTTGAAATGTA
TGAAGGTAAAGGATTAGTCGATAAAGACACTTTAATTAAAGGTGTTAAAAACGCGACTGCCTTAATTAGTTTATTATCTA
CAAACGTTGATAAAGATGTTATCGATGCTGGTAAAGACTTAAAAATCATTGCCAACTATGGCGCTGGTTTTAATAATATT
GATATCGAGTATGCCCGAGAAAAAAGTATAGATGTTACAAACACACCTAAAGCATCAACAAACGCGACTGCTGATTTAAC
AATTGGATTAGTACTTGCAGTAGCTCGTCGTATTGTTGAAGGGGACCAATTATCACGTACAACTGGATTTGACGGATGGG
CACCTTTATTTTTCAGAGGTAGAGAAGTATCTGGGAAAACAATCGGCATTATCGGTTTAGGTGAAATTGGTAGTGCAGTA
GCTCGTCGTGCAAGAGCATTTGACATGGATGTGCTATACACTGGACCTAATCGCAAAGAAGAAAAAGAACGAGAAATCGG
TGCAAAATATGTAGATTTAGATACACTATTAAAGAATGCAGATTTTATCACTATCAACGCTGCTTATAATCCTAAAATGC
ATCATTTAATTGATACAGAACAATTTAAAATGATGAAATCTACGGTGTATTTAATCAATGCCTCTCGTGGTCCAATCGTG
CACGAACAAGCACTAGTGCAAGCATTGAAAGATAATGAAATTGAAGGTGCTGCACTTGATGTATATGAATTTGAACCAGA
TATTACCGATGACTTAAAATCACTTAATAATGTAGTATTAACACCGCATATTGGTAATGCTACATTTGAAGCACGTGACA
TGATGTCTAAAATTGTTGCTAATGCTGCAATTAGTGCCGTTCAAGGTGAGAAACCACAATTTGTCGTTAACTAA

Upstream 100 bases:

>100_bases
ATCAAAAGTACGCGCTTACATCATTGATACACAAGACATCATGACATAGTTGTATACTGTCTAATCTTCTACTATACTAA
GATAAAAGGAGTGATCTTTT

Downstream 100 bases:

>100_bases
TAATTGAATTTATTATAATAACATTATAAAAACAAGAACCCCCATATGTTGAGCTCTCGTTTCAACATACGGGGGTTTTC
ATATTGTTGTATTATTTCTC

Product: D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding

Products: glycerate; NADP; NADPH; glyoxylate; 2-Dehydro-D-gluconate; 5-ketogluconate; L-idonate [C]

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKNATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNI
DIEYAREKSIDVTNTPKASTNATADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLIDTEQFKMMKSTVYLINASRGPIV
HEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLNNVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN

Sequences:

>Translated_317_residues
MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKNATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNI
DIEYAREKSIDVTNTPKASTNATADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLIDTEQFKMMKSTVYLINASRGPIV
HEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLNNVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN
>Mature_317_residues
MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKNATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNI
DIEYAREKSIDVTNTPKASTNATADLTIGLVLAVARRIVEGDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLIDTEQFKMMKSTVYLINASRGPIV
HEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLNNVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN

Specific function: Unknown

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family

Homologues:

Organism=Homo sapiens, GI6912396, Length=315, Percent_Identity=35.8730158730159, Blast_Score=194, Evalue=8e-50,
Organism=Homo sapiens, GI23308577, Length=318, Percent_Identity=33.0188679245283, Blast_Score=170, Evalue=1e-42,
Organism=Homo sapiens, GI4557497, Length=260, Percent_Identity=37.3076923076923, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI61743967, Length=260, Percent_Identity=37.3076923076923, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI145580575, Length=288, Percent_Identity=32.9861111111111, Blast_Score=148, Evalue=6e-36,
Organism=Homo sapiens, GI145580578, Length=263, Percent_Identity=35.361216730038, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI4557499, Length=263, Percent_Identity=35.361216730038, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI87082289, Length=313, Percent_Identity=36.1022364217252, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1787645, Length=245, Percent_Identity=34.2857142857143, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1789279, Length=281, Percent_Identity=32.0284697508897, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI87081824, Length=249, Percent_Identity=27.3092369477912, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788660, Length=256, Percent_Identity=26.171875, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17532191, Length=260, Percent_Identity=34.2307692307692, Blast_Score=144, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI25147481, Length=282, Percent_Identity=28.3687943262411, Blast_Score=122, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6320925, Length=323, Percent_Identity=33.1269349845201, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6322116, Length=303, Percent_Identity=34.6534653465347, Blast_Score=159, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6324055, Length=241, Percent_Identity=34.0248962655602, Blast_Score=155, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6324964, Length=312, Percent_Identity=23.3974358974359, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6321253, Length=280, Percent_Identity=24.6428571428571, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28571528, Length=317, Percent_Identity=36.2776025236593, Blast_Score=181, Evalue=7e-46,
Organism=Drosophila melanogaster, GI45552429, Length=305, Percent_Identity=33.7704918032787, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI45551003, Length=305, Percent_Identity=33.7704918032787, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24585514, Length=305, Percent_Identity=33.7704918032787, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI28574282, Length=305, Percent_Identity=33.7704918032787, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI28574284, Length=305, Percent_Identity=33.7704918032787, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI28574286, Length=313, Percent_Identity=33.5463258785943, Blast_Score=164, Evalue=8e-41,
Organism=Drosophila melanogaster, GI19921140, Length=318, Percent_Identity=33.0188679245283, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24646446, Length=267, Percent_Identity=32.2097378277154, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24646448, Length=267, Percent_Identity=32.2097378277154, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24646452, Length=267, Percent_Identity=32.2097378277154, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24646450, Length=267, Percent_Identity=32.2097378277154, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI62472511, Length=270, Percent_Identity=32.2222222222222, Blast_Score=128, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24585516, Length=304, Percent_Identity=25.9868421052632, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y2098_STAAN (Q7A417)

Other databases:

- EMBL:   BA000018
- PIR:   D90029
- RefSeq:   NP_375418.1
- HSSP:   O58320
- ProteinModelPortal:   Q7A417
- STRING:   Q7A417
- SWISS-2DPAGE:   Q7A417
- EnsemblBacteria:   EBSTAT00000002265
- GeneID:   1125023
- GenomeReviews:   BA000018_GR
- KEGG:   sau:SA2098
- eggNOG:   COG1052
- GeneTree:   EBGT00050000024534
- HOGENOM:   HBG731446
- OMA:   TAYIVNA
- ProtClustDB:   CLSK885881
- BioCyc:   SAUR158879:SA2098-MONOMER
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C

EC number: NA

Molecular weight: Translated: 34703; Mature: 34703

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1; PS00670 D_2_HYDROXYACID_DH_2; PS00671 D_2_HYDROXYACID_DH_3

Important sites: ACT_SITE 236-236 ACT_SITE 265-265 ACT_SITE 283-283 BINDING 260-260

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKNATALISLLSTNVDKDV
CCEEEEECCCHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
IDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNATADLTIGLVLAVARRIVE
HHCCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
GDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAVARRARAFDMDVLYTGPNRKE
CCCCCCCCCCCCCCCCEEECCCCCCCEEEEEECCHHHHHHHHHHHHCCEEEEEECCCCCH
EKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLIDTEQFKMMKSTVYLINASRGPIV
HHHHHHCCEEECHHHHHCCCCEEEEEECCCCCHHHHCCHHHHHHHHHEEEEEECCCCCCH
HEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLNNVVLTPHIGNATFEARDMMSKIVA
HHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHH
NAAISAVQGEKPQFVVN
HHHHHHHCCCCCCEEEC
>Mature Secondary Structure
MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKNATALISLLSTNVDKDV
CCEEEEECCCHHHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
IDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNATADLTIGLVLAVARRIVE
HHCCCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHC
GDQLSRTTGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAVARRARAFDMDVLYTGPNRKE
CCCCCCCCCCCCCCCCEEECCCCCCCEEEEEECCHHHHHHHHHHHHCCEEEEEECCCCCH
EKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLIDTEQFKMMKSTVYLINASRGPIV
HHHHHHCCEEECHHHHHCCCCEEEEEECCCCCHHHHCCHHHHHHHHHEEEEEECCCCCCH
HEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLNNVVLTPHIGNATFEARDMMSKIVA
HHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHH
NAAISAVQGEKPQFVVN
HHHHHHHCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: hydroxypyruvate; NADPH; glycolate; NADP; gluconate; 2,5-diketo-D-gluconate; 2-keto-L-gulonate [C]

Specific reaction: hydroxypyruvate + NADPH = glycerate + NADP glycolate + NADP = NADPH + glyoxylate NADP + gluconate = NADPH + 2-Dehydro-D-gluconate 2,5-diketo-D-gluconate + NADPH = 5-ketogluconate + NADP 2-keto-L-gulonate + NADPH = L-idonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11418146