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The map label for this gene is amiA [H]

Identifier: 150010523

GI number: 150010523

Start: 4808970

End: 4810091

Strand: Direct

Name: amiA [H]

Synonym: BDI_3970

Alternate gene names: 150010523

Gene position: 4808970-4810091 (Clockwise)

Preceding gene: 150010522

Following gene: 150010524

Centisome position: 99.95

GC content: 45.45

Gene sequence:

>1122_bases
TTGTTTTTTTTCTTGCCCGGACTTCAGGCGAGAGAGAAGACTTTTACGGTCGTGATCGATGCTGGGCATGGCGGTAAGGA
CCCGGGAGCTAGAGGGGCGAATGTTAATGAGAAGGAGATAAATCTCGCTGTCGCCTTAAAATTGGGTCGATTGATAGAGA
ATAACGCGGAGGATGTGCGTGTGATCTATACTCGTAAGACGGATCGTTTTATCGAGTTGGACGAACGTGCGAATATAGCG
AACCGCAATAAGGCGGATCTTTTCGTGTCTATCCATACGAATGCCGTAAAGCGTGGAAGTACCGTGCAAGGTACGGAGAC
GTATACGCTGGGTTTGGCCCGTAGCGAAGAGAACTTAGAGGTGGCTATGAGTGAGAACTCAGCGATCTTGTTGGAGGATA
ATTATCAACAACGGTATGAGGGATTTGACCCGAACTCTTCGGAGTCCTATATCATTTTCGAGTTTATGCAGAATAAGCAT
ATGGAGCAAAGTATTAGTCTGGCTTCCGAGATACAGAAATCGTTCGGGGCTTGTAAGCGGGTGGACCGTGGCGTAAGACA
GGCTGGTTTTTTGGTGCTTCGTAAAACGAGCATGCCCAGTGTTCTGGTCGAGTTGGGGTATATCTCGAATCGTCAAGAAG
AGCAGTTCATGAGAACCGCCGCCGGACAAAATAAATTGGCGGAAGCGTTGTACGATGCTTTTTGCAAATATAAGAAGAAT
TACGATCGTCGTAAAGGAAATATTTCCGGAGCTGTCGTTGCTCCCGTAGCGAATGAGAAAACTCCGCCTCCGGGGAGCGA
GGCTGATATCTTAAATAAAAAACAGCAAGCCACAAGACAGAAAACCTCAGTTTCTTCTACCACTTCCGTGAACAGGAACA
CGAAAGGAAAGGTTATATATAAGATACAATTCTTGACTTCCAATAAGAAGCTTCCTGCGAACTCCCGGTTATTTAAGGGG
TACAAGAACGTGGATTTCTATGTGGAGAAAGGTATCTATAAATACACGTATGGCGAGACTTCGGATTTTAACTCGATCCG
GAAGATGCGCCGAACGATCGCAAAAGATTTTAAAGATGCTTTCATCATCGCTTTTAAGGATGGGAAGAAGGTGAAATATT
AA

Upstream 100 bases:

>100_bases
TCTGTCAATCAGCAAAAAAGCGCTACATTTGTCTCCAAATTTGCAATAATTTTAGAAAGTGAGCAAAAAGTCTTTTCTAT
ATATTAACTTGCTATTTGCG

Downstream 100 bases:

>100_bases
TGATTTAGGAATAAATTATACATTATAAATAAAGGAAATGAAAAGTGTATTCACTAAAGAGGCGAAGATCGGACTTGTGA
CGATCGTAAGTCTGGCGTTA

Product: putative N-acetylmuramoyl-L-alanine amidase amiA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MFFFLPGLQAREKTFTVVIDAGHGGKDPGARGANVNEKEINLAVALKLGRLIENNAEDVRVIYTRKTDRFIELDERANIA
NRNKADLFVSIHTNAVKRGSTVQGTETYTLGLARSEENLEVAMSENSAILLEDNYQQRYEGFDPNSSESYIIFEFMQNKH
MEQSISLASEIQKSFGACKRVDRGVRQAGFLVLRKTSMPSVLVELGYISNRQEEQFMRTAAGQNKLAEALYDAFCKYKKN
YDRRKGNISGAVVAPVANEKTPPPGSEADILNKKQQATRQKTSVSSTTSVNRNTKGKVIYKIQFLTSNKKLPANSRLFKG
YKNVDFYVEKGIYKYTYGETSDFNSIRKMRRTIAKDFKDAFIIAFKDGKKVKY

Sequences:

>Translated_373_residues
MFFFLPGLQAREKTFTVVIDAGHGGKDPGARGANVNEKEINLAVALKLGRLIENNAEDVRVIYTRKTDRFIELDERANIA
NRNKADLFVSIHTNAVKRGSTVQGTETYTLGLARSEENLEVAMSENSAILLEDNYQQRYEGFDPNSSESYIIFEFMQNKH
MEQSISLASEIQKSFGACKRVDRGVRQAGFLVLRKTSMPSVLVELGYISNRQEEQFMRTAAGQNKLAEALYDAFCKYKKN
YDRRKGNISGAVVAPVANEKTPPPGSEADILNKKQQATRQKTSVSSTTSVNRNTKGKVIYKIQFLTSNKKLPANSRLFKG
YKNVDFYVEKGIYKYTYGETSDFNSIRKMRRTIAKDFKDAFIIAFKDGKKVKY
>Mature_373_residues
MFFFLPGLQAREKTFTVVIDAGHGGKDPGARGANVNEKEINLAVALKLGRLIENNAEDVRVIYTRKTDRFIELDERANIA
NRNKADLFVSIHTNAVKRGSTVQGTETYTLGLARSEENLEVAMSENSAILLEDNYQQRYEGFDPNSSESYIIFEFMQNKH
MEQSISLASEIQKSFGACKRVDRGVRQAGFLVLRKTSMPSVLVELGYISNRQEEQFMRTAAGQNKLAEALYDAFCKYKKN
YDRRKGNISGAVVAPVANEKTPPPGSEADILNKKQQATRQKTSVSSTTSVNRNTKGKVIYKIQFLTSNKKLPANSRLFKG
YKNVDFYVEKGIYKYTYGETSDFNSIRKMRRTIAKDFKDAFIIAFKDGKKVKY

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm. Note=Distributed throughout the periplasm in all cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=236, Percent_Identity=32.2033898305085, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI87082163, Length=239, Percent_Identity=31.3807531380753, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790611, Length=234, Percent_Identity=32.0512820512821, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 42161; Mature: 42161

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFFLPGLQAREKTFTVVIDAGHGGKDPGARGANVNEKEINLAVALKLGRLIENNAEDVR
CEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHEEEEEEEHHHHHHHCCCCCEE
VIYTRKTDRFIELDERANIANRNKADLFVSIHTNAVKRGSTVQGTETYTLGLARSEENLE
EEEEECCCCEEEECCCCCCCCCCCCEEEEEEECCHHCCCCCCCCCCEEEEEEEECCCCEE
VAMSENSAILLEDNYQQRYEGFDPNSSESYIIFEFMQNKHMEQSISLASEIQKSFGACKR
EEECCCCEEEEECCHHHHHCCCCCCCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHH
VDRGVRQAGFLVLRKTSMPSVLVELGYISNRQEEQFMRTAAGQNKLAEALYDAFCKYKKN
HHHHHHHHCEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
YDRRKGNISGAVVAPVANEKTPPPGSEADILNKKQQATRQKTSVSSTTSVNRNTKGKVIY
HHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
KIQFLTSNKKLPANSRLFKGYKNVDFYVEKGIYKYTYGETSDFNSIRKMRRTIAKDFKDA
EEEEEECCCCCCCCCHHHCCCCCCEEEEECCEEEEECCCCCCHHHHHHHHHHHHHCCCCE
FIIAFKDGKKVKY
EEEEEECCCCCCC
>Mature Secondary Structure
MFFFLPGLQAREKTFTVVIDAGHGGKDPGARGANVNEKEINLAVALKLGRLIENNAEDVR
CEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHEEEEEEEHHHHHHHCCCCCEE
VIYTRKTDRFIELDERANIANRNKADLFVSIHTNAVKRGSTVQGTETYTLGLARSEENLE
EEEEECCCCEEEECCCCCCCCCCCCEEEEEEECCHHCCCCCCCCCCEEEEEEEECCCCEE
VAMSENSAILLEDNYQQRYEGFDPNSSESYIIFEFMQNKHMEQSISLASEIQKSFGACKR
EEECCCCEEEEECCHHHHHCCCCCCCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHHHHHH
VDRGVRQAGFLVLRKTSMPSVLVELGYISNRQEEQFMRTAAGQNKLAEALYDAFCKYKKN
HHHHHHHHCEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
YDRRKGNISGAVVAPVANEKTPPPGSEADILNKKQQATRQKTSVSSTTSVNRNTKGKVIY
HHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
KIQFLTSNKKLPANSRLFKGYKNVDFYVEKGIYKYTYGETSDFNSIRKMRRTIAKDFKDA
EEEEEECCCCCCCCCHHHCCCCCCEEEEECCEEEEECCCCCCHHHHHHHHHHHHHCCCCE
FIIAFKDGKKVKY
EEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8300522; 9205837; 9278503; 1903834 [H]