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The map label for this gene is fusA [H]

Identifier: 150010331

GI number: 150010331

Start: 4588338

End: 4590494

Strand: Direct

Name: fusA [H]

Synonym: BDI_3765

Alternate gene names: 150010331

Gene position: 4588338-4590494 (Clockwise)

Preceding gene: 150010328

Following gene: 150010332

Centisome position: 95.36

GC content: 47.06

Gene sequence:

>2157_bases
ATGAAAGTATACCAAACAAATGAAATTAAGAACATCTCTATCTTGGGAAGTTCGGGCTCTGGGAAAACCACTCTCGCTGA
AGCTATGCTTTACGAGGGTGGAGTTATAAAACGTCGCGGCTCCGTTGAGGCAGGAAATACGGTGTCCGATTATTTCCCGG
TTGAGAAAGAGTATGGATACTCAGTGTTCTCAACCGTTTTCAGTGTAGAATGGCAGGATCGGAAGCTGAATTTCATCGAT
TGTCCGGGTTCGGATGATTTCGTGGGAGGAGCTGTTTCCGCTCTGAATGTCACGGATACGGCACTGATGGTATTGAATGC
CCAGTACGGTGTTGAGGTTGGAACCGTTAACCAGTTCCGGTATACTGAACAATTTCATAAACCGGTGATTTTCGTTGTCA
ATCAGCTTGATAATGATAAAGCCGACTATGAGGGAACCATCGCCCAGTTGAAAGACCGTTGGGGCAGTAAGATCGTTCCT
ATCCAATATCCGATTAGCTGCGGCTCTTCTTTCAATGCTGTGGTGGATGTATTAAAAATGAAGATGTATCGTTGGAAACC
GGAAGGTGGAGTTCCTGATGTATTGGAAATCCCCGCCGAGGAAATGGATAAGGCGATGGAGTTGCATCAAGCCTTGGTGG
AAGCCGCCGCTGAGAACGACGATATGTTGATGGAGAAATTCTTTGAGGAAGGTGCCTTGAGCGAGGATGATATGCGTGCC
GGTATCCGTGCGGGCTTGGTTACTCGCGGTATGTTCCCGGTATTCTGCGTATGTGCCGGTCGTGATATGTGTGTTCGTCG
TACGTTGGAGTTCTTGGGTAACGTGGTTCCTTGTACAGACAAGATGCCTCGTCTGATTACTACGGAAGGCGTAGAGGTGA
CTCCGGACTCGAATGGTCCTACTAGCTTGTTCTTCTTCAAGACTACCGTGGAACCTCATATTGGTCAGGTTTCCTATTTT
AAAGTGATATCAGGAAAAGTAAAAGAAGGTGACGATTTGATGAATGCCGATCGTGGTTCCAAGGAGCGTATCGCACAGTT
ATTTGCGGTAGCCGGACAAACTCGTACCCCGGTTACGGAAATGGTGGCGGGTGATATTGGAGCGACCGTTAAGTTAAAGG
ATGTTCGCCGTGGTAATACATTGAACGGAAAGGGTTGTGATTATCGTTTCGACTTCATTAAATATCCGGATCCGAAATAT
CGTCGTGCTATCAAGCCGGTGAATGAGGCTGACGCCGAGAAGATGATGGAAATCTTGATACGTATGCGTGAGGAAGACCC
GACATGGGTAATCGAGCAGTCCAAGGAATTGAAGCAGACAATCGTTTCCGGTCAAGGTGAGTTCCATCTTCGTACATTGA
AATGGAGAATCGAGAATAATGATAAATTGCCGATCGAATATCTGGAGCCGAAGATCCCGTATCGTGAGACGATTACGAAA
GCGGCTCGTGCGGATTACCGTCATAAGAAACAATCCGGTGGCGCAGGCCAGTTCGGTGAAGTTCATTTGATTATCGAGCC
TTATTATGAAGGTATGCCTGCTCCGGATACCTACCGTTTCGGCGGACAGGAGTATAAGATGAATGTTCGTGATACTCAAA
TAATTGATTTGGATTGGGGTGGTAAAGTCGTGTTCGTAAACTGTATCGTTGGTGGTGCTATCGACGCTCGTTTCTTGCCA
GCTATCTTGAAAGGTATCATGGCTCGTATGGAGCAAGGGCCGTTGACGGGTTCGTATGCTCGCGACGTGCGTATTTGCGT
ATACGATGGTAAGATGCACCCGGTAGACAGTAATGAAATCTCTTTCATGCTTGCCGGACGCAATGCGTTCAGTACCGCTT
TCAAGGAGGCTGGCCCTAAGATCTTGGAGCCTGTTTACGATGTCGAGGTTTCCGTTCCTGCCGATTATCTGGGAGATGTG
ATGAGCGACTTGCAAGGACGCCGTGCCTTGATTATGGGTATGAATAGCGAGAAGGGATTTGAGAAACTATTGGCGAAAGT
TCCTTTGAAAGAGATGTCTAACTATTCTACTTCACTAAGTTCCATTACGGGCGGACGTGCTTCCTTTACGATGAAGTTCG
CTAGTTACGAGTTAGTTCCTGCCGATGTACAAGAGAAGTTGTTAAAGGCTTACGAGGCTACTCAATCAGAAGAATAG

Upstream 100 bases:

>100_bases
CGATAATTGCTTTTTTCCTTTACAATCTCTAATTTGCGCCCTCGTGAAAGTGATTCACGCCAAATAAACGTATTGTTAAA
CTCAAATAAATCCTGATATC

Downstream 100 bases:

>100_bases
GTAAAGTAAAATGTCATCATAATTTTTTAATATTTAAAGTTAAACGAGAAAAGGCCGGCTTTGTGAAGAGCTGGCCTTTT
TGTTATGTCTTTTTCACTAG

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 718; Mature: 718

Protein sequence:

>718_residues
MKVYQTNEIKNISILGSSGSGKTTLAEAMLYEGGVIKRRGSVEAGNTVSDYFPVEKEYGYSVFSTVFSVEWQDRKLNFID
CPGSDDFVGGAVSALNVTDTALMVLNAQYGVEVGTVNQFRYTEQFHKPVIFVVNQLDNDKADYEGTIAQLKDRWGSKIVP
IQYPISCGSSFNAVVDVLKMKMYRWKPEGGVPDVLEIPAEEMDKAMELHQALVEAAAENDDMLMEKFFEEGALSEDDMRA
GIRAGLVTRGMFPVFCVCAGRDMCVRRTLEFLGNVVPCTDKMPRLITTEGVEVTPDSNGPTSLFFFKTTVEPHIGQVSYF
KVISGKVKEGDDLMNADRGSKERIAQLFAVAGQTRTPVTEMVAGDIGATVKLKDVRRGNTLNGKGCDYRFDFIKYPDPKY
RRAIKPVNEADAEKMMEILIRMREEDPTWVIEQSKELKQTIVSGQGEFHLRTLKWRIENNDKLPIEYLEPKIPYRETITK
AARADYRHKKQSGGAGQFGEVHLIIEPYYEGMPAPDTYRFGGQEYKMNVRDTQIIDLDWGGKVVFVNCIVGGAIDARFLP
AILKGIMARMEQGPLTGSYARDVRICVYDGKMHPVDSNEISFMLAGRNAFSTAFKEAGPKILEPVYDVEVSVPADYLGDV
MSDLQGRRALIMGMNSEKGFEKLLAKVPLKEMSNYSTSLSSITGGRASFTMKFASYELVPADVQEKLLKAYEATQSEE

Sequences:

>Translated_718_residues
MKVYQTNEIKNISILGSSGSGKTTLAEAMLYEGGVIKRRGSVEAGNTVSDYFPVEKEYGYSVFSTVFSVEWQDRKLNFID
CPGSDDFVGGAVSALNVTDTALMVLNAQYGVEVGTVNQFRYTEQFHKPVIFVVNQLDNDKADYEGTIAQLKDRWGSKIVP
IQYPISCGSSFNAVVDVLKMKMYRWKPEGGVPDVLEIPAEEMDKAMELHQALVEAAAENDDMLMEKFFEEGALSEDDMRA
GIRAGLVTRGMFPVFCVCAGRDMCVRRTLEFLGNVVPCTDKMPRLITTEGVEVTPDSNGPTSLFFFKTTVEPHIGQVSYF
KVISGKVKEGDDLMNADRGSKERIAQLFAVAGQTRTPVTEMVAGDIGATVKLKDVRRGNTLNGKGCDYRFDFIKYPDPKY
RRAIKPVNEADAEKMMEILIRMREEDPTWVIEQSKELKQTIVSGQGEFHLRTLKWRIENNDKLPIEYLEPKIPYRETITK
AARADYRHKKQSGGAGQFGEVHLIIEPYYEGMPAPDTYRFGGQEYKMNVRDTQIIDLDWGGKVVFVNCIVGGAIDARFLP
AILKGIMARMEQGPLTGSYARDVRICVYDGKMHPVDSNEISFMLAGRNAFSTAFKEAGPKILEPVYDVEVSVPADYLGDV
MSDLQGRRALIMGMNSEKGFEKLLAKVPLKEMSNYSTSLSSITGGRASFTMKFASYELVPADVQEKLLKAYEATQSEE
>Mature_718_residues
MKVYQTNEIKNISILGSSGSGKTTLAEAMLYEGGVIKRRGSVEAGNTVSDYFPVEKEYGYSVFSTVFSVEWQDRKLNFID
CPGSDDFVGGAVSALNVTDTALMVLNAQYGVEVGTVNQFRYTEQFHKPVIFVVNQLDNDKADYEGTIAQLKDRWGSKIVP
IQYPISCGSSFNAVVDVLKMKMYRWKPEGGVPDVLEIPAEEMDKAMELHQALVEAAAENDDMLMEKFFEEGALSEDDMRA
GIRAGLVTRGMFPVFCVCAGRDMCVRRTLEFLGNVVPCTDKMPRLITTEGVEVTPDSNGPTSLFFFKTTVEPHIGQVSYF
KVISGKVKEGDDLMNADRGSKERIAQLFAVAGQTRTPVTEMVAGDIGATVKLKDVRRGNTLNGKGCDYRFDFIKYPDPKY
RRAIKPVNEADAEKMMEILIRMREEDPTWVIEQSKELKQTIVSGQGEFHLRTLKWRIENNDKLPIEYLEPKIPYRETITK
AARADYRHKKQSGGAGQFGEVHLIIEPYYEGMPAPDTYRFGGQEYKMNVRDTQIIDLDWGGKVVFVNCIVGGAIDARFLP
AILKGIMARMEQGPLTGSYARDVRICVYDGKMHPVDSNEISFMLAGRNAFSTAFKEAGPKILEPVYDVEVSVPADYLGDV
MSDLQGRRALIMGMNSEKGFEKLLAKVPLKEMSNYSTSLSSITGGRASFTMKFASYELVPADVQEKLLKAYEATQSEE

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=731, Percent_Identity=31.6005471956224, Blast_Score=328, Evalue=8e-90,
Organism=Homo sapiens, GI19923640, Length=738, Percent_Identity=25.3387533875339, Blast_Score=218, Evalue=2e-56,
Organism=Homo sapiens, GI25306283, Length=386, Percent_Identity=26.1658031088083, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI25306287, Length=392, Percent_Identity=25.2551020408163, Blast_Score=118, Evalue=3e-26,
Organism=Homo sapiens, GI4503483, Length=443, Percent_Identity=22.7990970654628, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1789738, Length=718, Percent_Identity=32.7298050139276, Blast_Score=383, Evalue=1e-107,
Organism=Escherichia coli, GI1790835, Length=428, Percent_Identity=26.1682242990654, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI48994988, Length=173, Percent_Identity=27.1676300578035, Blast_Score=70, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17533571, Length=714, Percent_Identity=29.8319327731092, Blast_Score=322, Evalue=6e-88,
Organism=Caenorhabditis elegans, GI17556745, Length=745, Percent_Identity=22.9530201342282, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17506493, Length=447, Percent_Identity=24.1610738255034, Blast_Score=99, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323098, Length=729, Percent_Identity=29.6296296296296, Blast_Score=299, Evalue=1e-81,
Organism=Saccharomyces cerevisiae, GI6322359, Length=797, Percent_Identity=24.7176913425345, Blast_Score=226, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6324707, Length=429, Percent_Identity=24.7086247086247, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320593, Length=429, Percent_Identity=24.7086247086247, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24582462, Length=722, Percent_Identity=31.0249307479224, Blast_Score=332, Evalue=4e-91,
Organism=Drosophila melanogaster, GI221458488, Length=748, Percent_Identity=23.1283422459893, Blast_Score=216, Evalue=4e-56,
Organism=Drosophila melanogaster, GI24585711, Length=456, Percent_Identity=24.1228070175439, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24585713, Length=456, Percent_Identity=24.1228070175439, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24585709, Length=456, Percent_Identity=24.1228070175439, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 80100; Mature: 80100

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVYQTNEIKNISILGSSGSGKTTLAEAMLYEGGVIKRRGSVEAGNTVSDYFPVEKEYGY
CCCEECCCCCEEEEEECCCCCCHHHHHHHHHCCCEEEECCCCCCCCCHHHCCCCCHHCCH
SVFSTVFSVEWQDRKLNFIDCPGSDDFVGGAVSALNVTDTALMVLNAQYGVEVGTVNQFR
HHHHHHHEEEECCCCEEEEECCCCCCCCCCCEEEEECCCEEEEEEECCCCEEECCCHHHH
YTEQFHKPVIFVVNQLDNDKADYEGTIAQLKDRWGSKIVPIQYPISCGSSFNAVVDVLKM
HHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHH
KMYRWKPEGGVPDVLEIPAEEMDKAMELHQALVEAAAENDDMLMEKFFEEGALSEDDMRA
HHHEECCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHH
GIRAGLVTRGMFPVFCVCAGRDMCVRRTLEFLGNVVPCTDKMPRLITTEGVEVTPDSNGP
HHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCEEECCCCCCC
TSLFFFKTTVEPHIGQVSYFKVISGKVKEGDDLMNADRGSKERIAQLFAVAGQTRTPVTE
EEEEEEEEECCCCCCCEEEEHHHCCCCCCCCHHHCCCCCCHHHHHHHHHHCCCCCCCHHH
MVAGDIGATVKLKDVRRGNTLNGKGCDYRFDFIKYPDPKYRRAIKPVNEADAEKMMEILI
HHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCHHHHHHCCCCCCCCHHHHHHHHH
RMREEDPTWVIEQSKELKQTIVSGQGEFHLRTLKWRIENNDKLPIEYLEPKIPYRETITK
HHCCCCCCEEECCHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCHHCCCCCCCHHHHHHH
AARADYRHKKQSGGAGQFGEVHLIIEPYYEGMPAPDTYRFGGQEYKMNVRDTQIIDLDWG
HHHHHHHHHHCCCCCCCCCEEEEEEEEHHCCCCCCCCCCCCCCEEEEECCCCEEEEECCC
GKVVFVNCIVGGAIDARFLPAILKGIMARMEQGPLTGSYARDVRICVYDGKMHPVDSNEI
CEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEECCCCCCCE
SFMLAGRNAFSTAFKEAGPKILEPVYDVEVSVPADYLGDVMSDLQGRRALIMGMNSEKGF
EEEEECCHHHHHHHHHCCCHHHCHHHCEEEECCHHHHHHHHHHCCCCEEEEEECCCCHHH
EKLLAKVPLKEMSNYSTSLSSITGGRASFTMKFASYELVPADVQEKLLKAYEATQSEE
HHHHHHCCHHHHHCCHHHHHHCCCCCCEEEEEEECEEECCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKVYQTNEIKNISILGSSGSGKTTLAEAMLYEGGVIKRRGSVEAGNTVSDYFPVEKEYGY
CCCEECCCCCEEEEEECCCCCCHHHHHHHHHCCCEEEECCCCCCCCCHHHCCCCCHHCCH
SVFSTVFSVEWQDRKLNFIDCPGSDDFVGGAVSALNVTDTALMVLNAQYGVEVGTVNQFR
HHHHHHHEEEECCCCEEEEECCCCCCCCCCCEEEEECCCEEEEEEECCCCEEECCCHHHH
YTEQFHKPVIFVVNQLDNDKADYEGTIAQLKDRWGSKIVPIQYPISCGSSFNAVVDVLKM
HHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHH
KMYRWKPEGGVPDVLEIPAEEMDKAMELHQALVEAAAENDDMLMEKFFEEGALSEDDMRA
HHHEECCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHH
GIRAGLVTRGMFPVFCVCAGRDMCVRRTLEFLGNVVPCTDKMPRLITTEGVEVTPDSNGP
HHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCEEECCCCCCC
TSLFFFKTTVEPHIGQVSYFKVISGKVKEGDDLMNADRGSKERIAQLFAVAGQTRTPVTE
EEEEEEEEECCCCCCCEEEEHHHCCCCCCCCHHHCCCCCCHHHHHHHHHHCCCCCCCHHH
MVAGDIGATVKLKDVRRGNTLNGKGCDYRFDFIKYPDPKYRRAIKPVNEADAEKMMEILI
HHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCHHHHHHCCCCCCCCHHHHHHHHH
RMREEDPTWVIEQSKELKQTIVSGQGEFHLRTLKWRIENNDKLPIEYLEPKIPYRETITK
HHCCCCCCEEECCHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCHHCCCCCCCHHHHHHH
AARADYRHKKQSGGAGQFGEVHLIIEPYYEGMPAPDTYRFGGQEYKMNVRDTQIIDLDWG
HHHHHHHHHHCCCCCCCCCEEEEEEEEHHCCCCCCCCCCCCCCEEEEECCCCEEEEECCC
GKVVFVNCIVGGAIDARFLPAILKGIMARMEQGPLTGSYARDVRICVYDGKMHPVDSNEI
CEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEECCCCCCCE
SFMLAGRNAFSTAFKEAGPKILEPVYDVEVSVPADYLGDVMSDLQGRRALIMGMNSEKGF
EEEEECCHHHHHHHHHCCCHHHCHHHCEEEECCHHHHHHHHHHCCCCEEEEEECCCCHHH
EKLLAKVPLKEMSNYSTSLSSITGGRASFTMKFASYELVPADVQEKLLKAYEATQSEE
HHHHHHCCHHHHHCCHHHHHHCCCCCCEEEEEEECEEECCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA