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The map label for this gene is ahpF [H]

Identifier: 150009978

GI number: 150009978

Start: 4130608

End: 4132161

Strand: Direct

Name: ahpF [H]

Synonym: BDI_3397

Alternate gene names: 150009978

Gene position: 4130608-4132161 (Clockwise)

Preceding gene: 150009977

Following gene: 150009979

Centisome position: 85.85

GC content: 46.91

Gene sequence:

>1554_bases
GTGTTAGAATCAACATTAAAAGAACAATTAAAAGGTATATTCGCAGGGTTAAATGCGAATTATACCTTTGATATATCGAT
CTCTCCTGCGCATGAAAGTAGACAAGAGTTGTTGGATTTATTAGGTGATGTTGCCTCTTGTTCGGATAAAATATCTATGC
GAGTATCTGATGGGGATGGATTGGAATTCATTTTATTAAAGGATGGAGCGAAAACGGGGATTAAGTTTCGTGGAGTACCT
AATGGACACGAGTTTACTTCCTTGCTGTTGGCGATCTTGAATTCAGATGGGAAAGGCAAGAATTTTCCGGACGAGAGCAT
TTGTAATCGTGTTAAAGCCTTGAATGGCCCGATACATCTGACGACCTATGTCTCTTTGACTTGTACGAATTGCCCGGATG
TGGTACAGGCATTAAACGCTATGACGACTTTAAATCCTCAGATCCATCATGAGATGGTAGATGGGGCTATCAATCAGGCG
GAAGTTGATGCCTTGAAGATACAAGGCGTCCCCTCTGTCTTTGCGGATGGAAAACTGATCCATGTAGGACGTGGGGAATT
CGGTGAATTGTTATCGAAGCTGGAAGCTCAGTATGGAATCAATGAGAGCCTGACAGAGAAGACGGTAAAACGATATGACG
TGGTTGTGGTCGGTGGAGGGCCGGCTGGTGCTTCTTCCGCTATTTATTCCGCACGAAAGGGGCTGAGTGTCGCTGTCGTG
GCGGAGCGTATCGGCGGGCAGGTGAAGGAGACGGTAGGAATTGAGAATCTGATTTCAGTTCCGGAGACAACAGGTACCCA
GTTGGCTGACAATCTGCGCTTGCATATGCGGCAATATCCGATTGACCTGCTGGAGCATCGTCGTATAGAGAAGGTAACGA
TAGAAGGAAACGAGAAGGTTTTGTCTACGGCTGGAGGTGAGATATTCAAGGCTCCGGCGGTTATTATCGCTACGGGCGCT
AGCTGGCGTAAATTGAATGTACCGGGTGAGGCAGAGTATATAGGGCGTGGAGTGGCTTTTTGTCCTCATTGCGACGGTCC
GTTTTATAAGGGAAAACATGTCGCCGTGGTTGGCGGTGGAAATTCCGGTATCGAGGCGGCTATTGATTTGGCGGGGATCT
GTTCGAAAGTAACCGTTTTGGAGTTTATGGATGAGTTAAAAGCGGATCAAGTCTTGCAAGAGAAAGCGAAGAGTTTGCCG
AATGTCGAGATCTTTGTTAGTTCACAAACAACCGAGGTGGTTGGAAATGGCGACAAGGTAACCGGTATCCGTACAAAGGA
TCGTAAGACAGGGGAAGAGCGTGTTATTCATTTAGACGGTATTTTTGTTCAAATCGGCTTAGCCGCTAATAGTGGTGTCT
TTAAGGAGATTGTCGAAACAAATCGTCCCGGTGAGATCGTGATTGACGCACATTGCCGGACGAATGTCCCGGGTATTTAT
GCTGCGGGCGACGTTTCCACCGTACCCTTTAAGCAAATCATTATTTCTATGGGAGAAGGCGCCAAAGCGGCCCTTTCGGC
CTTTGAAGATCGGGTACGTGGCGTACTTGATTGA

Upstream 100 bases:

>100_bases
GATGGTGAGGTTTGTCCCGCTAAATGGAAGAAAGGGGAGGCTACCTTGAAACCTAGCATTGACTTGGTAGGGAAAATCTA
AATAATTAAGAGTATAGATC

Downstream 100 bases:

>100_bases
GGAAAGTACCTACAAATAGGTAATTTTTGAACAGCGTATCTCTTTTATCGGTTATATTACTATATTTGCGATCGAGAGAA
AAGAGATGGATAGATTAAGA

Product: alkyl hydroperoxide reductase subunit F

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MLESTLKEQLKGIFAGLNANYTFDISISPAHESRQELLDLLGDVASCSDKISMRVSDGDGLEFILLKDGAKTGIKFRGVP
NGHEFTSLLLAILNSDGKGKNFPDESICNRVKALNGPIHLTTYVSLTCTNCPDVVQALNAMTTLNPQIHHEMVDGAINQA
EVDALKIQGVPSVFADGKLIHVGRGEFGELLSKLEAQYGINESLTEKTVKRYDVVVVGGGPAGASSAIYSARKGLSVAVV
AERIGGQVKETVGIENLISVPETTGTQLADNLRLHMRQYPIDLLEHRRIEKVTIEGNEKVLSTAGGEIFKAPAVIIATGA
SWRKLNVPGEAEYIGRGVAFCPHCDGPFYKGKHVAVVGGGNSGIEAAIDLAGICSKVTVLEFMDELKADQVLQEKAKSLP
NVEIFVSSQTTEVVGNGDKVTGIRTKDRKTGEERVIHLDGIFVQIGLAANSGVFKEIVETNRPGEIVIDAHCRTNVPGIY
AAGDVSTVPFKQIIISMGEGAKAALSAFEDRVRGVLD

Sequences:

>Translated_517_residues
MLESTLKEQLKGIFAGLNANYTFDISISPAHESRQELLDLLGDVASCSDKISMRVSDGDGLEFILLKDGAKTGIKFRGVP
NGHEFTSLLLAILNSDGKGKNFPDESICNRVKALNGPIHLTTYVSLTCTNCPDVVQALNAMTTLNPQIHHEMVDGAINQA
EVDALKIQGVPSVFADGKLIHVGRGEFGELLSKLEAQYGINESLTEKTVKRYDVVVVGGGPAGASSAIYSARKGLSVAVV
AERIGGQVKETVGIENLISVPETTGTQLADNLRLHMRQYPIDLLEHRRIEKVTIEGNEKVLSTAGGEIFKAPAVIIATGA
SWRKLNVPGEAEYIGRGVAFCPHCDGPFYKGKHVAVVGGGNSGIEAAIDLAGICSKVTVLEFMDELKADQVLQEKAKSLP
NVEIFVSSQTTEVVGNGDKVTGIRTKDRKTGEERVIHLDGIFVQIGLAANSGVFKEIVETNRPGEIVIDAHCRTNVPGIY
AAGDVSTVPFKQIIISMGEGAKAALSAFEDRVRGVLD
>Mature_517_residues
MLESTLKEQLKGIFAGLNANYTFDISISPAHESRQELLDLLGDVASCSDKISMRVSDGDGLEFILLKDGAKTGIKFRGVP
NGHEFTSLLLAILNSDGKGKNFPDESICNRVKALNGPIHLTTYVSLTCTNCPDVVQALNAMTTLNPQIHHEMVDGAINQA
EVDALKIQGVPSVFADGKLIHVGRGEFGELLSKLEAQYGINESLTEKTVKRYDVVVVGGGPAGASSAIYSARKGLSVAVV
AERIGGQVKETVGIENLISVPETTGTQLADNLRLHMRQYPIDLLEHRRIEKVTIEGNEKVLSTAGGEIFKAPAVIIATGA
SWRKLNVPGEAEYIGRGVAFCPHCDGPFYKGKHVAVVGGGNSGIEAAIDLAGICSKVTVLEFMDELKADQVLQEKAKSLP
NVEIFVSSQTTEVVGNGDKVTGIRTKDRKTGEERVIHLDGIFVQIGLAANSGVFKEIVETNRPGEIVIDAHCRTNVPGIY
AAGDVSTVPFKQIIISMGEGAKAALSAFEDRVRGVLD

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=521, Percent_Identity=47.2168905950096, Blast_Score=459, Evalue=1e-130,
Organism=Escherichia coli, GI1787114, Length=321, Percent_Identity=33.9563862928349, Blast_Score=162, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6320560, Length=307, Percent_Identity=33.5504885993485, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6321898, Length=322, Percent_Identity=31.3664596273292, Blast_Score=152, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 55228; Mature: 55228

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLESTLKEQLKGIFAGLNANYTFDISISPAHESRQELLDLLGDVASCSDKISMRVSDGDG
CCHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
LEFILLKDGAKTGIKFRGVPNGHEFTSLLLAILNSDGKGKNFPDESICNRVKALNGPIHL
EEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEE
TTYVSLTCTNCPDVVQALNAMTTLNPQIHHEMVDGAINQAEVDALKIQGVPSVFADGKLI
EEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHCCEEEECCCCCHHCCCEEE
HVGRGEFGELLSKLEAQYGINESLTEKTVKRYDVVVVGGGPAGASSAIYSARKGLSVAVV
EECCCCHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHCCCEEEEE
AERIGGQVKETVGIENLISVPETTGTQLADNLRLHMRQYPIDLLEHRRIEKVTIEGNEKV
HHHHCCHHHHHHCHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCEEEEEECCCCEE
LSTAGGEIFKAPAVIIATGASWRKLNVPGEAEYIGRGVAFCPHCDGPFYKGKHVAVVGGG
EECCCCCEEECCEEEEEECCCCEEECCCCCHHHHCCCCEECCCCCCCCCCCCEEEEEECC
NSGIEAAIDLAGICSKVTVLEFMDELKADQVLQEKAKSLPNVEIFVSSQTTEVVGNGDKV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCEE
TGIRTKDRKTGEERVIHLDGIFVQIGLAANSGVFKEIVETNRPGEIVIDAHCRTNVPGIY
EEEECCCCCCCCCEEEEEEEEEEEEEECCCCCHHHHHHHCCCCCEEEEECCCCCCCCCEE
AAGDVSTVPFKQIIISMGEGAKAALSAFEDRVRGVLD
ECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLESTLKEQLKGIFAGLNANYTFDISISPAHESRQELLDLLGDVASCSDKISMRVSDGDG
CCHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
LEFILLKDGAKTGIKFRGVPNGHEFTSLLLAILNSDGKGKNFPDESICNRVKALNGPIHL
EEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEE
TTYVSLTCTNCPDVVQALNAMTTLNPQIHHEMVDGAINQAEVDALKIQGVPSVFADGKLI
EEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCHHCCEEEECCCCCHHCCCEEE
HVGRGEFGELLSKLEAQYGINESLTEKTVKRYDVVVVGGGPAGASSAIYSARKGLSVAVV
EECCCCHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHCCCEEEEE
AERIGGQVKETVGIENLISVPETTGTQLADNLRLHMRQYPIDLLEHRRIEKVTIEGNEKV
HHHHCCHHHHHHCHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHCCCCEEEEEECCCCEE
LSTAGGEIFKAPAVIIATGASWRKLNVPGEAEYIGRGVAFCPHCDGPFYKGKHVAVVGGG
EECCCCCEEECCEEEEEECCCCEEECCCCCHHHHCCCCEECCCCCCCCCCCCEEEEEECC
NSGIEAAIDLAGICSKVTVLEFMDELKADQVLQEKAKSLPNVEIFVSSQTTEVVGNGDKV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCEE
TGIRTKDRKTGEERVIHLDGIFVQIGLAANSGVFKEIVETNRPGEIVIDAHCRTNVPGIY
EEEECCCCCCCCCEEEEEEEEEEEEEECCCCCHHHHHHHCCCCCEEEEECCCCCCCCCEE
AAGDVSTVPFKQIIISMGEGAKAALSAFEDRVRGVLD
ECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]