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The map label for this gene is eno [H]

Identifier: 150009976

GI number: 150009976

Start: 4128521

End: 4129801

Strand: Direct

Name: eno [H]

Synonym: BDI_3395

Alternate gene names: 150009976

Gene position: 4128521-4129801 (Clockwise)

Preceding gene: 150009975

Following gene: 150009977

Centisome position: 85.81

GC content: 46.37

Gene sequence:

>1281_bases
ATGAAAATAGAAAAGATTACAGGACGTGAGATCCTAGATTCAAGAGGTAACCCGACAGTAGAGGTTGATATTTTACTAGA
GTCCGGCGTTATGGGCCGTGCGTCTGTGCCTTCCGGTGCGTCCACCGGCGAGAACGAGGCTTTGGAGTTGCGTGATGGCG
ATAAGAAGCGTTATGGTGGTAAAGGCGTATTGAAAGCGGTTGAGAATATCAATACGATTATCGCTCCGGCATTGAAAGGG
ATGTCTGCCTTGGATCAGATAGGTATCGATCATGCGATGTTGGCTTTGGACGGTACGAAAACTAAATCGAAACTAGGTGC
GAACGCTATCCTTGGCGTTTCTTTAGCTGTAGCGAAAGCTGCCGCAAATTATTTGGATATTCCTTTATATAGATATATTG
GTGGAACGAATACCTACGTGATGCCTGTTCCGATGATGAATATAATCAATGGCGGTTCGCATAGCGACGCTCCGATCGCT
TTCCAAGAGTTTATGATTCGTCCGGTAGGTGCCGCTTCTTTCAAGGAAGGCTTACGTATGGGCGCTGAGGTATTCCATGC
GTTGAAAAAGGTATTGCACGATCGTGGGTTAAGTACCGCTGTTGGTGATGAGGGTGGTTTCGCCCCGAATTTGGAAGGTA
CGGAAGATGCCTTGAACTCAATTATCGCCGCTATCAAGGCGGCTGGTTATGAACCGGGCAAGGATGTGATGATCGGTATG
GATTGCGCTTCTTCTGAGTTTTATAAGGATGGAATCTACGATTACACGAAGTTTGAAGGAGAAAAAGGAGTGAAACGTAC
TTCCGACGAACAAGTGGATTATCTGGAAAAGCTGATCAACGAATACCCGATCGATTCCATTGAGGATGGTATGAGTGAGA
ATGATTGGGCTGGTTGGAAGAAACTGACAGATCGTATTGGTAACCATTGCCAATTAGTCGGAGATGATTTGTTCGTGACA
AATGTAGAGTTCCTTTCAAGGGGTATTAAAGAAGGTTGCGGTAACTCAATCTTGATCAAGGTAAATCAGATCGGTTCCTT
GACGGAGACACTGAACGCTATCGAGATGGCTCACCGTCATGGATACACGACCGTTACTTCACACCGTTCCGGTGAGACGG
AAGACGCTACGATCGCCGATATCGCTGTCGCTACCAATAGCGGACAGATCAAGACGGGATCATTAAGTCGTTCGGACCGT
ATGGCTAAATATAACCAGCTGCTTCGTATCGAGGAGGAATTGGGAGACCGTGCCGTATACGGATACAAAAGAATCAATTA
A

Upstream 100 bases:

>100_bases
CTATATTCAGGTATTATTGAGGCGAAAATAGCAATTTGTGGTGATTTCACGGTTCAAGTAATCCATCTATATTTGCGTCA
GTTTAAAAAACGTAGAAGCA

Downstream 100 bases:

>100_bases
GTATATAAAGTAAATTATAATCGCTAATTATGTAGTAATCCAAAACCCAAGATATTGAAGGGCTTATCCGTGAGGATAGG
CCCTTTTTATTGATTGATAA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKIEKITGREILDSRGNPTVEVDILLESGVMGRASVPSGASTGENEALELRDGDKKRYGGKGVLKAVENINTIIAPALKG
MSALDQIGIDHAMLALDGTKTKSKLGANAILGVSLAVAKAAANYLDIPLYRYIGGTNTYVMPVPMMNIINGGSHSDAPIA
FQEFMIRPVGAASFKEGLRMGAEVFHALKKVLHDRGLSTAVGDEGGFAPNLEGTEDALNSIIAAIKAAGYEPGKDVMIGM
DCASSEFYKDGIYDYTKFEGEKGVKRTSDEQVDYLEKLINEYPIDSIEDGMSENDWAGWKKLTDRIGNHCQLVGDDLFVT
NVEFLSRGIKEGCGNSILIKVNQIGSLTETLNAIEMAHRHGYTTVTSHRSGETEDATIADIAVATNSGQIKTGSLSRSDR
MAKYNQLLRIEEELGDRAVYGYKRIN

Sequences:

>Translated_426_residues
MKIEKITGREILDSRGNPTVEVDILLESGVMGRASVPSGASTGENEALELRDGDKKRYGGKGVLKAVENINTIIAPALKG
MSALDQIGIDHAMLALDGTKTKSKLGANAILGVSLAVAKAAANYLDIPLYRYIGGTNTYVMPVPMMNIINGGSHSDAPIA
FQEFMIRPVGAASFKEGLRMGAEVFHALKKVLHDRGLSTAVGDEGGFAPNLEGTEDALNSIIAAIKAAGYEPGKDVMIGM
DCASSEFYKDGIYDYTKFEGEKGVKRTSDEQVDYLEKLINEYPIDSIEDGMSENDWAGWKKLTDRIGNHCQLVGDDLFVT
NVEFLSRGIKEGCGNSILIKVNQIGSLTETLNAIEMAHRHGYTTVTSHRSGETEDATIADIAVATNSGQIKTGSLSRSDR
MAKYNQLLRIEEELGDRAVYGYKRIN
>Mature_426_residues
MKIEKITGREILDSRGNPTVEVDILLESGVMGRASVPSGASTGENEALELRDGDKKRYGGKGVLKAVENINTIIAPALKG
MSALDQIGIDHAMLALDGTKTKSKLGANAILGVSLAVAKAAANYLDIPLYRYIGGTNTYVMPVPMMNIINGGSHSDAPIA
FQEFMIRPVGAASFKEGLRMGAEVFHALKKVLHDRGLSTAVGDEGGFAPNLEGTEDALNSIIAAIKAAGYEPGKDVMIGM
DCASSEFYKDGIYDYTKFEGEKGVKRTSDEQVDYLEKLINEYPIDSIEDGMSENDWAGWKKLTDRIGNHCQLVGDDLFVT
NVEFLSRGIKEGCGNSILIKVNQIGSLTETLNAIEMAHRHGYTTVTSHRSGETEDATIADIAVATNSGQIKTGSLSRSDR
MAKYNQLLRIEEELGDRAVYGYKRIN

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=54.4186046511628, Blast_Score=440, Evalue=1e-123,
Organism=Homo sapiens, GI301897477, Length=433, Percent_Identity=53.3487297921478, Blast_Score=427, Evalue=1e-119,
Organism=Homo sapiens, GI301897469, Length=433, Percent_Identity=53.3487297921478, Blast_Score=427, Evalue=1e-119,
Organism=Homo sapiens, GI4503571, Length=430, Percent_Identity=52.7906976744186, Blast_Score=421, Evalue=1e-118,
Organism=Homo sapiens, GI301897479, Length=431, Percent_Identity=47.7958236658933, Blast_Score=364, Evalue=1e-101,
Organism=Homo sapiens, GI169201331, Length=347, Percent_Identity=27.9538904899135, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI169201757, Length=347, Percent_Identity=27.9538904899135, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI239744207, Length=347, Percent_Identity=27.9538904899135, Blast_Score=112, Evalue=6e-25,
Organism=Escherichia coli, GI1789141, Length=429, Percent_Identity=62.7039627039627, Blast_Score=501, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI71995829, Length=429, Percent_Identity=55.9440559440559, Blast_Score=426, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17536383, Length=429, Percent_Identity=55.9440559440559, Blast_Score=425, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI32563855, Length=187, Percent_Identity=50.2673796791444, Blast_Score=175, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6323985, Length=437, Percent_Identity=49.4279176201373, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6324974, Length=437, Percent_Identity=49.4279176201373, Blast_Score=406, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6324969, Length=437, Percent_Identity=49.4279176201373, Blast_Score=406, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6321693, Length=432, Percent_Identity=50.2314814814815, Blast_Score=405, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6321968, Length=432, Percent_Identity=50.2314814814815, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580918, Length=425, Percent_Identity=52.2352941176471, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580916, Length=425, Percent_Identity=52.2352941176471, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580920, Length=425, Percent_Identity=52.2352941176471, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580914, Length=425, Percent_Identity=52.2352941176471, Blast_Score=397, Evalue=1e-110,
Organism=Drosophila melanogaster, GI281360527, Length=425, Percent_Identity=52.2352941176471, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI17137654, Length=425, Percent_Identity=52.2352941176471, Blast_Score=395, Evalue=1e-110,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45996; Mature: 45996

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIEKITGREILDSRGNPTVEVDILLESGVMGRASVPSGASTGENEALELRDGDKKRYGG
CCCCCCCCHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCCHHCCC
KGVLKAVENINTIIAPALKGMSALDQIGIDHAMLALDGTKTKSKLGANAILGVSLAVAKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHHH
AANYLDIPLYRYIGGTNTYVMPVPMMNIINGGSHSDAPIAFQEFMIRPVGAASFKEGLRM
HHHHHCCCHHHHCCCCCCEEECCCHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHH
GAEVFHALKKVLHDRGLSTAVGDEGGFAPNLEGTEDALNSIIAAIKAAGYEPGKDVMIGM
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEC
DCASSEFYKDGIYDYTKFEGEKGVKRTSDEQVDYLEKLINEYPIDSIEDGMSENDWAGWK
CCCCCHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHH
KLTDRIGNHCQLVGDDLFVTNVEFLSRGIKEGCGNSILIKVNQIGSLTETLNAIEMAHRH
HHHHHCCCCEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHC
GYTTVTSHRSGETEDATIADIAVATNSGQIKTGSLSRSDRMAKYNQLLRIEEELGDRAVY
CCEEEECCCCCCCCCCEEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCEEC
GYKRIN
CEECCC
>Mature Secondary Structure
MKIEKITGREILDSRGNPTVEVDILLESGVMGRASVPSGASTGENEALELRDGDKKRYGG
CCCCCCCCHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEECCCCCCHHCCC
KGVLKAVENINTIIAPALKGMSALDQIGIDHAMLALDGTKTKSKLGANAILGVSLAVAKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHHH
AANYLDIPLYRYIGGTNTYVMPVPMMNIINGGSHSDAPIAFQEFMIRPVGAASFKEGLRM
HHHHHCCCHHHHCCCCCCEEECCCHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHH
GAEVFHALKKVLHDRGLSTAVGDEGGFAPNLEGTEDALNSIIAAIKAAGYEPGKDVMIGM
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEC
DCASSEFYKDGIYDYTKFEGEKGVKRTSDEQVDYLEKLINEYPIDSIEDGMSENDWAGWK
CCCCCHHHHCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHH
KLTDRIGNHCQLVGDDLFVTNVEFLSRGIKEGCGNSILIKVNQIGSLTETLNAIEMAHRH
HHHHHCCCCEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHC
GYTTVTSHRSGETEDATIADIAVATNSGQIKTGSLSRSDRMAKYNQLLRIEEELGDRAVY
CCEEEECCCCCCCCCCEEEEEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCEEC
GYKRIN
CEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA