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The map label for this gene is parE [H]

Identifier: 150009930

GI number: 150009930

Start: 4057268

End: 4059133

Strand: Direct

Name: parE [H]

Synonym: BDI_3347

Alternate gene names: 150009930

Gene position: 4057268-4059133 (Clockwise)

Preceding gene: 150009929

Following gene: 150009931

Centisome position: 84.33

GC content: 46.95

Gene sequence:

>1866_bases
ATGGATGAGCTGAATTCAACGATACAACAACCTACGGAATATAACGATGATGACATCAAGACACTGGACTGGATGGAGCA
TATCCGCCGGCGTCCCGGTATGTATATCGGAAAACTGGGGGACGGTAGCTATGCCGATGATGGTATCTATGTGTTGTTGA
AGGAGGTGCTGGATAACTCTATCGACGAGTACATGATGGGGTATGGCAAAAGTATCGAGGTGACTATCGAGGAAGGTACG
GTAGCCGTGCGGGATTATGGCCGTGGTGTCCCTCTGGGTAAGGTCGTGGATGTGTCTAGCAAGATGAATACCGGCGCTAA
ATACGATAGCAAGGCGTTTAAGAAATCCGTGGGTTTGAATGGTGTGGGTATCAAGGCGGTGAATGCCTTGAGTAGTTATT
TCTTGATAACCAGCTACCGTGACGGAGAGTGTAAGCGGGTGGAATATAGTAAGGCGATCGTCACGGAGGAATCGGATATC
CAACCGACCGGGGAGGCGAACGGTACGCAGGTGTTTTTTGTCCCGGATCGGGAGATCTTTAAGGATTATCATTATAAGGA
CGAGTTTATCGAGGGGCTATTAAAGAACTATGTCTTCTTGAACTCGGGACTTTCCATTATTTACAACGGCAAGCGTTTCT
ATTCCCGCAACGGTCTGGTGGATTTGTTGAACGAGAATATGACCACCGAGCCTCTTTACCCGATCATCCACCTGAAAGGG
GAGGATATAGAGGTGGTTATCACGCATAGCAACCAATATGGAGAGGAATATTATTCGTTCGTGAACGGCCAGCATACCAC
CCAAGGCGGTACGCACCTTTCTGCTTTCAAGGAGTCTATAGGCCGTGTCATCAAGGAATATTACAATAAGAATTTCGAGT
ATTCGGATATTCGTGCGGGTATCGTGGCCGCTATCAGCATAAAAGTGGAGGAGCCTGTATTCGAGAGCCAGACAAAGACC
AAGCTCGGTTCGAAAGATATAGGACCGGACGGCCCTACGGTAGCGAAGTTTATCGGTGATTTCATCAAGAATGACCTGGA
TAACTATTTGCATAAGAATATGGAGACTTCGGAGCTTTTGCTGAAGAAGATCTTGGAGTCCGAGAAAGAGCGCAAGGCGA
TCGCCGGTGTTACGAAGCTGGCCCGCGAGCGTGCGAAGAAAGCGAATTTACATAATAAGAAACTAAGGGATTGCCGGATT
CACCTGACCGACTCGAAAGGGGATTTGCTAGAGGAAAGCAGTATCTTTATCACGGAGGGTGACTCGGCTAGCGGTTCTAT
CACGAAGAGCCGTAACCCGAATTACCAAGCTGTATTTAGTTTGAGGGGTAAGCCTTTGAATAGTTATGGCTTGACAAAGA
AGATCGTTTACGAGAACGAGGAGTTTAACTTGCTTCAAGCGGCCTTGAATATTGAGGATGGCTTGGAAGGTTTGCGGTAT
AATAAGGTGATTATCGCGACAGATGCCGATGTGGACGGGATGCATATCCGTTTATTGTTGATTACCTTCTTCTTGCAATT
CTTCCCCGATTTGATTAAGCGTAATCATGTTTATATCTTGCAAACCCCGTTATTCCGGGTACGTAACCGGCAGAAGACGT
GGTATTGTTATTCAGAGGAGGAACGCTTGGCTGCCATAGCCGCTGCCGGGAAGAATCCGGAGATCACCCGATTCAAAGGT
CTGGGAGAGATCTCTCCCGATGAGTTCAAGCATTTTATCGGCAAGGATATACGCCTTGATCCGGTGACAATGAAGAAAGA
GGACCTCGTGAAAGATATGCTCGAGTTCTATATGGGGAAGAATACCATGGAGCGACAGAACTTTATTATCGATAACTTAG
TGGTTGAAGAAGATGTCATCAATTGA

Upstream 100 bases:

>100_bases
CGGAGCTGGAGACGAATAGCGCCGTACAAGCGCAATGGGATTATTTCAAGCGAGAGCATCATAAATCAAGAAGAGCGTAT
ATTAAAAAGTTATAAGAGAT

Downstream 100 bases:

>100_bases
AAATAAACGTATTGCGCTTTTTCCCGGTACCTTCGATCCTTTTACGATCGGGCATCAATCCTTGGTAAGGCGTGGGTTGG
AACTGGTGGACGAGATCGTG

Product: DNA topoisomerase IV subunit B

Products: Double stranded DNA [C]

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 621; Mature: 621

Protein sequence:

>621_residues
MDELNSTIQQPTEYNDDDIKTLDWMEHIRRRPGMYIGKLGDGSYADDGIYVLLKEVLDNSIDEYMMGYGKSIEVTIEEGT
VAVRDYGRGVPLGKVVDVSSKMNTGAKYDSKAFKKSVGLNGVGIKAVNALSSYFLITSYRDGECKRVEYSKAIVTEESDI
QPTGEANGTQVFFVPDREIFKDYHYKDEFIEGLLKNYVFLNSGLSIIYNGKRFYSRNGLVDLLNENMTTEPLYPIIHLKG
EDIEVVITHSNQYGEEYYSFVNGQHTTQGGTHLSAFKESIGRVIKEYYNKNFEYSDIRAGIVAAISIKVEEPVFESQTKT
KLGSKDIGPDGPTVAKFIGDFIKNDLDNYLHKNMETSELLLKKILESEKERKAIAGVTKLARERAKKANLHNKKLRDCRI
HLTDSKGDLLEESSIFITEGDSASGSITKSRNPNYQAVFSLRGKPLNSYGLTKKIVYENEEFNLLQAALNIEDGLEGLRY
NKVIIATDADVDGMHIRLLLITFFLQFFPDLIKRNHVYILQTPLFRVRNRQKTWYCYSEEERLAAIAAAGKNPEITRFKG
LGEISPDEFKHFIGKDIRLDPVTMKKEDLVKDMLEFYMGKNTMERQNFIIDNLVVEEDVIN

Sequences:

>Translated_621_residues
MDELNSTIQQPTEYNDDDIKTLDWMEHIRRRPGMYIGKLGDGSYADDGIYVLLKEVLDNSIDEYMMGYGKSIEVTIEEGT
VAVRDYGRGVPLGKVVDVSSKMNTGAKYDSKAFKKSVGLNGVGIKAVNALSSYFLITSYRDGECKRVEYSKAIVTEESDI
QPTGEANGTQVFFVPDREIFKDYHYKDEFIEGLLKNYVFLNSGLSIIYNGKRFYSRNGLVDLLNENMTTEPLYPIIHLKG
EDIEVVITHSNQYGEEYYSFVNGQHTTQGGTHLSAFKESIGRVIKEYYNKNFEYSDIRAGIVAAISIKVEEPVFESQTKT
KLGSKDIGPDGPTVAKFIGDFIKNDLDNYLHKNMETSELLLKKILESEKERKAIAGVTKLARERAKKANLHNKKLRDCRI
HLTDSKGDLLEESSIFITEGDSASGSITKSRNPNYQAVFSLRGKPLNSYGLTKKIVYENEEFNLLQAALNIEDGLEGLRY
NKVIIATDADVDGMHIRLLLITFFLQFFPDLIKRNHVYILQTPLFRVRNRQKTWYCYSEEERLAAIAAAGKNPEITRFKG
LGEISPDEFKHFIGKDIRLDPVTMKKEDLVKDMLEFYMGKNTMERQNFIIDNLVVEEDVIN
>Mature_621_residues
MDELNSTIQQPTEYNDDDIKTLDWMEHIRRRPGMYIGKLGDGSYADDGIYVLLKEVLDNSIDEYMMGYGKSIEVTIEEGT
VAVRDYGRGVPLGKVVDVSSKMNTGAKYDSKAFKKSVGLNGVGIKAVNALSSYFLITSYRDGECKRVEYSKAIVTEESDI
QPTGEANGTQVFFVPDREIFKDYHYKDEFIEGLLKNYVFLNSGLSIIYNGKRFYSRNGLVDLLNENMTTEPLYPIIHLKG
EDIEVVITHSNQYGEEYYSFVNGQHTTQGGTHLSAFKESIGRVIKEYYNKNFEYSDIRAGIVAAISIKVEEPVFESQTKT
KLGSKDIGPDGPTVAKFIGDFIKNDLDNYLHKNMETSELLLKKILESEKERKAIAGVTKLARERAKKANLHNKKLRDCRI
HLTDSKGDLLEESSIFITEGDSASGSITKSRNPNYQAVFSLRGKPLNSYGLTKKIVYENEEFNLLQAALNIEDGLEGLRY
NKVIIATDADVDGMHIRLLLITFFLQFFPDLIKRNHVYILQTPLFRVRNRQKTWYCYSEEERLAAIAAAGKNPEITRFKG
LGEISPDEFKHFIGKDIRLDPVTMKKEDLVKDMLEFYMGKNTMERQNFIIDNLVVEEDVIN

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=624, Percent_Identity=27.724358974359, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI19913406, Length=623, Percent_Identity=26.645264847512, Blast_Score=120, Evalue=4e-27,
Organism=Escherichia coli, GI48994957, Length=567, Percent_Identity=33.5097001763668, Blast_Score=263, Evalue=3e-71,
Organism=Escherichia coli, GI1789408, Length=646, Percent_Identity=29.2569659442724, Blast_Score=257, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI17535065, Length=615, Percent_Identity=26.1788617886179, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI212645845, Length=552, Percent_Identity=26.0869565217391, Blast_Score=106, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6324241, Length=589, Percent_Identity=25.2971137521222, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17136538, Length=624, Percent_Identity=26.7628205128205, Blast_Score=121, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.3 [C]

Molecular weight: Translated: 70648; Mature: 70648

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDELNSTIQQPTEYNDDDIKTLDWMEHIRRRPGMYIGKLGDGSYADDGIYVLLKEVLDNS
CCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCC
IDEYMMGYGKSIEVTIEEGTVAVRDYGRGVPLGKVVDVSSKMNTGAKYDSKAFKKSVGLN
HHHHHHCCCCEEEEEEECCEEEEEECCCCCCCCCEEECCHHCCCCCCCCHHHHHHHCCCC
GVGIKAVNALSSYFLITSYRDGECKRVEYSKAIVTEESDIQPTGEANGTQVFFVPDREIF
CCCHHHHHHHHHEEEEEECCCCCCCEEEHHHHEECCCCCCCCCCCCCCEEEEEECCHHHH
KDYHYKDEFIEGLLKNYVFLNSGLSIIYNGKRFYSRNGLVDLLNENMTTEPLYPIIHLKG
HHCCCHHHHHHHHHHHHEEECCCCEEEECCCEEECCCCHHHHHCCCCCCCCCCEEEEECC
EDIEVVITHSNQYGEEYYSFVNGQHTTQGGTHLSAFKESIGRVIKEYYNKNFEYSDIRAG
CCEEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCC
IVAAISIKVEEPVFESQTKTKLGSKDIGPDGPTVAKFIGDFIKNDLDNYLHKNMETSELL
EEEEEEEEECCCCHHCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LKKILESEKERKAIAGVTKLARERAKKANLHNKKLRDCRIHLTDSKGDLLEESSIFITEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCEEEECC
DSASGSITKSRNPNYQAVFSLRGKPLNSYGLTKKIVYENEEFNLLQAALNIEDGLEGLRY
CCCCCCEECCCCCCEEEEEEECCCCCCCCCCEEEEEEECCCHHHHHHHHCHHHHCCCCEE
NKVIIATDADVDGMHIRLLLITFFLQFFPDLIKRNHVYILQTPLFRVRNRQKTWYCYSEE
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCEEEEECCC
ERLAAIAAAGKNPEITRFKGLGEISPDEFKHFIGKDIRLDPVTMKKEDLVKDMLEFYMGK
CCEEEEEECCCCCCCEEECCCCCCCHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHCC
NTMERQNFIIDNLVVEEDVIN
CCHHHHHHHHHHEEEHHHCCC
>Mature Secondary Structure
MDELNSTIQQPTEYNDDDIKTLDWMEHIRRRPGMYIGKLGDGSYADDGIYVLLKEVLDNS
CCCHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCC
IDEYMMGYGKSIEVTIEEGTVAVRDYGRGVPLGKVVDVSSKMNTGAKYDSKAFKKSVGLN
HHHHHHCCCCEEEEEEECCEEEEEECCCCCCCCCEEECCHHCCCCCCCCHHHHHHHCCCC
GVGIKAVNALSSYFLITSYRDGECKRVEYSKAIVTEESDIQPTGEANGTQVFFVPDREIF
CCCHHHHHHHHHEEEEEECCCCCCCEEEHHHHEECCCCCCCCCCCCCCEEEEEECCHHHH
KDYHYKDEFIEGLLKNYVFLNSGLSIIYNGKRFYSRNGLVDLLNENMTTEPLYPIIHLKG
HHCCCHHHHHHHHHHHHEEECCCCEEEECCCEEECCCCHHHHHCCCCCCCCCCEEEEECC
EDIEVVITHSNQYGEEYYSFVNGQHTTQGGTHLSAFKESIGRVIKEYYNKNFEYSDIRAG
CCEEEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHCC
IVAAISIKVEEPVFESQTKTKLGSKDIGPDGPTVAKFIGDFIKNDLDNYLHKNMETSELL
EEEEEEEEECCCCHHCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LKKILESEKERKAIAGVTKLARERAKKANLHNKKLRDCRIHLTDSKGDLLEESSIFITEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCEEEECC
DSASGSITKSRNPNYQAVFSLRGKPLNSYGLTKKIVYENEEFNLLQAALNIEDGLEGLRY
CCCCCCEECCCCCCEEEEEEECCCCCCCCCCEEEEEEECCCHHHHHHHHCHHHHCCCCEE
NKVIIATDADVDGMHIRLLLITFFLQFFPDLIKRNHVYILQTPLFRVRNRQKTWYCYSEE
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHCCCCCEEEEECCC
ERLAAIAAAGKNPEITRFKGLGEISPDEFKHFIGKDIRLDPVTMKKEDLVKDMLEFYMGK
CCEEEEEECCCCCCCEEECCCCCCCHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHCC
NTMERQNFIIDNLVVEEDVIN
CCHHHHHHHHHHEEEHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Double stranded DNA; DNA [C]

Specific reaction: ATP dependent breakage followed by passage and rejoining of double stranded DNA Protein + DNA = Protein-DNA [C]

General reaction: Isomerization [C]

Inhibitor: CJ-12, 371; CJ-12, 372; Coumermycin; Doxorubicin; Sitafloxacin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685; 7812434 [H]