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The map label for this gene is dusB [H]

Identifier: 150009773

GI number: 150009773

Start: 3859712

End: 3860701

Strand: Reverse

Name: dusB [H]

Synonym: BDI_3188

Alternate gene names: 150009773

Gene position: 3860701-3859712 (Counterclockwise)

Preceding gene: 150009774

Following gene: 150009772

Centisome position: 80.24

GC content: 50.51

Gene sequence:

>990_bases
ATGAAGATTGGTTCAATAGATTTAGGGGAGCGTCCGGTATTCTTGGCGCCTATGGAGGACGTGACGGATATCTCGTTTCG
TTTGATGTGCAAACGCTTCGGAGCGGACATGGTATATACGGAGTTCGTATCGAGCGACGCGTTGATCCGCAGTGTGAACA
AAACACAGCAAAAACTAAATGTCTCCGATGATGAGCGTCCGGTCGCTATCCAGATTTACGGCAAGGACGTAGCCTCGATG
GTAGAGGCCGCACGTATTTGCGAGGAAGCTCGTCCGGATATTTTAGATATAAACTTTGGTTGCCCCGTAAAGAAAGTAGC
GGGCAAGGGAGCCGGAGCCGGCATGCTACGCAATATCCCGTTGATGCTGGAGATCACGAGAGAGGTGGTGAAAGCCGTGA
ACATCCCCGTCACCGTGAAAACCCGTTTGGGATGGGATGCCGACAACCGCGTTATCGTGGATCTGGCCGAGCAACTACAG
GATTGCGGTATCGCCGCCCTCTCCATCCACGGACGTACACGGGCGCAAATGTACACGGGCGAGGCGGATTGGACCTTGAT
CCGTGCCGTAAAAAATAACCCTCGCATGCAAATCCCTATCATCGGGAATGGCGACGTGACTTCCGCCGAGATATGCAAGC
AGCGTTTCGAGGACTATGGCGTAGACGCCGTAATGATCGGTAGGGCTAGCATAGGCCGCCCTTGGATCTTCCGGGAGGTA
AAACACTATCTTACGACCGGGGAATTATTGCCACAAGAGCCTTTCTCTTGGTATCTGGATATCCTAAAAAAACAAGTGGA
GCAAAGCGTGGAACGATTGGACGAGCGTAGGGGAATCTTACATATTCGCCGTCATCTGGCAGCTACTCCACTCTTCAAAG
GTATTACCGATTTCAAGCAAACACGGATCGCCATGCTACGTGCGGAGACTGTTAACGAATTATTCGAAATTATGAATAAC
ATTCCTAAAAAGTACGGAATTTCAGAATAA

Upstream 100 bases:

>100_bases
AAAACACCTGTTCCATCTACCGATCATCTTACGAGATGTAAAAATTATTCCCCAAAAAGAAGTACTTTTGTAATCTCTAT
TTAGTTAGCAAACAGAAGGT

Downstream 100 bases:

>100_bases
CAAGCTTGATATATATCAAATTGAAACCTATTTGCGATATCTTTACAATAAAATGTTACAAATACTATTGACGCATATTA
TAAAACCCCTTATATTTGCA

Product: putative TIM-barrel enzyme

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKIGSIDLGERPVFLAPMEDVTDISFRLMCKRFGADMVYTEFVSSDALIRSVNKTQQKLNVSDDERPVAIQIYGKDVASM
VEAARICEEARPDILDINFGCPVKKVAGKGAGAGMLRNIPLMLEITREVVKAVNIPVTVKTRLGWDADNRVIVDLAEQLQ
DCGIAALSIHGRTRAQMYTGEADWTLIRAVKNNPRMQIPIIGNGDVTSAEICKQRFEDYGVDAVMIGRASIGRPWIFREV
KHYLTTGELLPQEPFSWYLDILKKQVEQSVERLDERRGILHIRRHLAATPLFKGITDFKQTRIAMLRAETVNELFEIMNN
IPKKYGISE

Sequences:

>Translated_329_residues
MKIGSIDLGERPVFLAPMEDVTDISFRLMCKRFGADMVYTEFVSSDALIRSVNKTQQKLNVSDDERPVAIQIYGKDVASM
VEAARICEEARPDILDINFGCPVKKVAGKGAGAGMLRNIPLMLEITREVVKAVNIPVTVKTRLGWDADNRVIVDLAEQLQ
DCGIAALSIHGRTRAQMYTGEADWTLIRAVKNNPRMQIPIIGNGDVTSAEICKQRFEDYGVDAVMIGRASIGRPWIFREV
KHYLTTGELLPQEPFSWYLDILKKQVEQSVERLDERRGILHIRRHLAATPLFKGITDFKQTRIAMLRAETVNELFEIMNN
IPKKYGISE
>Mature_329_residues
MKIGSIDLGERPVFLAPMEDVTDISFRLMCKRFGADMVYTEFVSSDALIRSVNKTQQKLNVSDDERPVAIQIYGKDVASM
VEAARICEEARPDILDINFGCPVKKVAGKGAGAGMLRNIPLMLEITREVVKAVNIPVTVKTRLGWDADNRVIVDLAEQLQ
DCGIAALSIHGRTRAQMYTGEADWTLIRAVKNNPRMQIPIIGNGDVTSAEICKQRFEDYGVDAVMIGRASIGRPWIFREV
KHYLTTGELLPQEPFSWYLDILKKQVEQSVERLDERRGILHIRRHLAATPLFKGITDFKQTRIAMLRAETVNELFEIMNN
IPKKYGISE

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=230, Percent_Identity=31.304347826087, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI239788462, Length=279, Percent_Identity=29.7491039426523, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI40807366, Length=228, Percent_Identity=28.9473684210526, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI31742496, Length=233, Percent_Identity=26.6094420600858, Blast_Score=102, Evalue=3e-22,
Organism=Homo sapiens, GI8923374, Length=244, Percent_Identity=28.2786885245902, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1789660, Length=287, Percent_Identity=41.1149825783972, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1788462, Length=327, Percent_Identity=30.8868501529052, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI145693211, Length=302, Percent_Identity=27.1523178807947, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17543114, Length=294, Percent_Identity=27.891156462585, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17507177, Length=253, Percent_Identity=26.8774703557312, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI25144369, Length=224, Percent_Identity=26.7857142857143, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17510279, Length=252, Percent_Identity=28.968253968254, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323560, Length=315, Percent_Identity=26.031746031746, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323433, Length=319, Percent_Identity=26.3322884012539, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323437, Length=231, Percent_Identity=25.1082251082251, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585320, Length=248, Percent_Identity=31.8548387096774, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24580595, Length=236, Percent_Identity=29.2372881355932, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI19920448, Length=236, Percent_Identity=29.2372881355932, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI19921524, Length=250, Percent_Identity=30, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI45549423, Length=247, Percent_Identity=30.7692307692308, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37080; Mature: 37080

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGSIDLGERPVFLAPMEDVTDISFRLMCKRFGADMVYTEFVSSDALIRSVNKTQQKLN
CCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
VSDDERPVAIQIYGKDVASMVEAARICEEARPDILDINFGCPVKKVAGKGAGAGMLRNIP
CCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHCCCCCCCHHHHCCC
LMLEITREVVKAVNIPVTVKTRLGWDADNRVIVDLAEQLQDCGIAALSIHGRTRAQMYTG
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEEECCCCEEEEEEC
EADWTLIRAVKNNPRMQIPIIGNGDVTSAEICKQRFEDYGVDAVMIGRASIGRPWIFREV
CCCCEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHH
KHYLTTGELLPQEPFSWYLDILKKQVEQSVERLDERRGILHIRRHLAATPLFKGITDFKQ
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
TRIAMLRAETVNELFEIMNNIPKKYGISE
HHHHHHHHHHHHHHHHHHHHCHHHCCCCC
>Mature Secondary Structure
MKIGSIDLGERPVFLAPMEDVTDISFRLMCKRFGADMVYTEFVSSDALIRSVNKTQQKLN
CCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
VSDDERPVAIQIYGKDVASMVEAARICEEARPDILDINFGCPVKKVAGKGAGAGMLRNIP
CCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHCCCCCCCHHHHCCC
LMLEITREVVKAVNIPVTVKTRLGWDADNRVIVDLAEQLQDCGIAALSIHGRTRAQMYTG
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHCCEEEEEECCCCEEEEEEC
EADWTLIRAVKNNPRMQIPIIGNGDVTSAEICKQRFEDYGVDAVMIGRASIGRPWIFREV
CCCCEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHH
KHYLTTGELLPQEPFSWYLDILKKQVEQSVERLDERRGILHIRRHLAATPLFKGITDFKQ
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
TRIAMLRAETVNELFEIMNNIPKKYGISE
HHHHHHHHHHHHHHHHHHHHCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]