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The map label for this gene is purD [H]

Identifier: 150009680

GI number: 150009680

Start: 3711989

End: 3713260

Strand: Direct

Name: purD [H]

Synonym: BDI_3095

Alternate gene names: 150009680

Gene position: 3711989-3713260 (Clockwise)

Preceding gene: 150009679

Following gene: 150009681

Centisome position: 77.15

GC content: 48.74

Gene sequence:

>1272_bases
ATGAATATATTGTTATTAGGCTCTGGTGGGCGTGAGCATGCGATCGCTTGGAAGATCGCTCAAAGCCCGAAAGTTGATAA
GTTATTTATCGCTCCCGGTAATGCGGGAACTGCGTTGGTAGGAACGAACGTGGATATCAAAGCGGATGATTTTGCCGCTA
TCAAGGATTTTGTGTTGGCGAACCAAGTGAATATGGTCGTGGTAGGTCCCGAGGACCCATTGGTGAAGGGTGTTTATGAT
TATTTCAAGAAGGATGAGGCATTAAGCTCCATCCCCGTGATCGGCCCGAGTGGGGAAGGTGCCCAATTGGAAGGTAGCAA
GGATTTCGCCAAGGCGTTCATGATGCGTCATAATATCCCGACTGCTCGTTATAAGAGTATTTCCGTTGAAAACTTAGAGG
AAGGTTATGCGTTCTTGGAATCTCTTTCCGCTCCTTATGTATTGAAAGCGGATGGATTGGCTGCCGGTAAAGGGGTACTG
ATTATCGATAACTTGGAGGAAGCCAAGAAAGAGCTGGGAGATATGCTGGGCGGAATGTTTGGCGACGCGAGCAAGACGGT
CGTGATCGAGGAGTTCTTGGATGGTATCGAATGCTCCGTATTCGTCTTGACGGATGGCAACGATTACAAGGTGCTTCCGG
TAGCGAAAGATTATAAGCGTATCGGCGAGGGCAATACGGGCTTGAATACCGGTGGCATGGGAGCTGTTTCTCCGGTTCCT
TTCGCTGATGAGGTCTTTATGGCGAAGGTGGACGAGCGTATTATCCGTCCTACCGTAGAGGGATTGAAAGCGGAGCATAT
CGATTATAAAGGTTTCATCTTCTTGGGTCTGATCAATGTGAAGAATGAGCCGATGGTTATAGAATACAATTGTCGTATGG
GTGATCCGGAGACGGAGGTAGTCATGCTTCGTATCCAAAGCGATTTGGTTGATTTGCTGGAAGGGGTAGCGGAAGGTAAT
TTAGCGGGGCGTACATTGGTACAAGATCCTCGTGCGGCGGTTACGGTTATGTTGGTAAGCGGCGGTTATCCGGAGGCTTA
CGAGAAAAATAAGGTAATCACGGGATTGGATGGCGTATCCTCGAACAATATAGTGTTCCATGCCGGAACGAAGCTGGTAG
ACGGCCAGATCCTGACGAATGGCGGCCGTGTGATCGCGGTCAGTTCGTATGGGGCTACTAAGGAAGAGGCTTTGGCCCAA
TCTTTCGCCGGAGCTAAGCAGATCGATTTCGATAAGAAGTATTTCCGTCGGGATATCGGGTTTGATTTATAA

Upstream 100 bases:

>100_bases
GCATAACGTGACCGGTAAGGACCGTCCGCATTTGCATGAGAAGATTACGAGGTATTTCGATGATTACTTGAAAAATTGAG
AATTGAAAATTAGAAGAGAT

Downstream 100 bases:

>100_bases
TTGCGTTTATCGAGAACGCCGGTCGGCATTTGCAGTGAACGTCAGTCGGCGTTTACGGTGAGTACCGGTCGGCGTTCACT
ACGAACGTCGCTCCGGTGCT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MNILLLGSGGREHAIAWKIAQSPKVDKLFIAPGNAGTALVGTNVDIKADDFAAIKDFVLANQVNMVVVGPEDPLVKGVYD
YFKKDEALSSIPVIGPSGEGAQLEGSKDFAKAFMMRHNIPTARYKSISVENLEEGYAFLESLSAPYVLKADGLAAGKGVL
IIDNLEEAKKELGDMLGGMFGDASKTVVIEEFLDGIECSVFVLTDGNDYKVLPVAKDYKRIGEGNTGLNTGGMGAVSPVP
FADEVFMAKVDERIIRPTVEGLKAEHIDYKGFIFLGLINVKNEPMVIEYNCRMGDPETEVVMLRIQSDLVDLLEGVAEGN
LAGRTLVQDPRAAVTVMLVSGGYPEAYEKNKVITGLDGVSSNNIVFHAGTKLVDGQILTNGGRVIAVSSYGATKEEALAQ
SFAGAKQIDFDKKYFRRDIGFDL

Sequences:

>Translated_423_residues
MNILLLGSGGREHAIAWKIAQSPKVDKLFIAPGNAGTALVGTNVDIKADDFAAIKDFVLANQVNMVVVGPEDPLVKGVYD
YFKKDEALSSIPVIGPSGEGAQLEGSKDFAKAFMMRHNIPTARYKSISVENLEEGYAFLESLSAPYVLKADGLAAGKGVL
IIDNLEEAKKELGDMLGGMFGDASKTVVIEEFLDGIECSVFVLTDGNDYKVLPVAKDYKRIGEGNTGLNTGGMGAVSPVP
FADEVFMAKVDERIIRPTVEGLKAEHIDYKGFIFLGLINVKNEPMVIEYNCRMGDPETEVVMLRIQSDLVDLLEGVAEGN
LAGRTLVQDPRAAVTVMLVSGGYPEAYEKNKVITGLDGVSSNNIVFHAGTKLVDGQILTNGGRVIAVSSYGATKEEALAQ
SFAGAKQIDFDKKYFRRDIGFDL
>Mature_423_residues
MNILLLGSGGREHAIAWKIAQSPKVDKLFIAPGNAGTALVGTNVDIKADDFAAIKDFVLANQVNMVVVGPEDPLVKGVYD
YFKKDEALSSIPVIGPSGEGAQLEGSKDFAKAFMMRHNIPTARYKSISVENLEEGYAFLESLSAPYVLKADGLAAGKGVL
IIDNLEEAKKELGDMLGGMFGDASKTVVIEEFLDGIECSVFVLTDGNDYKVLPVAKDYKRIGEGNTGLNTGGMGAVSPVP
FADEVFMAKVDERIIRPTVEGLKAEHIDYKGFIFLGLINVKNEPMVIEYNCRMGDPETEVVMLRIQSDLVDLLEGVAEGN
LAGRTLVQDPRAAVTVMLVSGGYPEAYEKNKVITGLDGVSSNNIVFHAGTKLVDGQILTNGGRVIAVSSYGATKEEALAQ
SFAGAKQIDFDKKYFRRDIGFDL

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=426, Percent_Identity=40.8450704225352, Blast_Score=321, Evalue=1e-87,
Organism=Homo sapiens, GI4503915, Length=426, Percent_Identity=40.8450704225352, Blast_Score=321, Evalue=1e-87,
Organism=Homo sapiens, GI209869995, Length=426, Percent_Identity=40.8450704225352, Blast_Score=321, Evalue=1e-87,
Organism=Homo sapiens, GI209869993, Length=426, Percent_Identity=40.8450704225352, Blast_Score=321, Evalue=1e-87,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=47.6635514018692, Blast_Score=376, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17567511, Length=423, Percent_Identity=41.6075650118203, Blast_Score=330, Evalue=8e-91,
Organism=Saccharomyces cerevisiae, GI6321203, Length=442, Percent_Identity=41.8552036199095, Blast_Score=328, Evalue=1e-90,
Organism=Drosophila melanogaster, GI62471619, Length=432, Percent_Identity=42.5925925925926, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24582400, Length=432, Percent_Identity=42.5925925925926, Blast_Score=305, Evalue=3e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45475; Mature: 45475

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILLLGSGGREHAIAWKIAQSPKVDKLFIAPGNAGTALVGTNVDIKADDFAAIKDFVLA
CEEEEEECCCCCCEEEEEECCCCCCCEEEEECCCCCCEEEECCEEECCCCHHHHHHHHHH
NQVNMVVVGPEDPLVKGVYDYFKKDEALSSIPVIGPSGEGAQLEGSKDFAKAFMMRHNIP
CCCCEEEECCCCCHHHHHHHHHHCCCHHHCCCEECCCCCCCEECCCHHHHHHHHHHCCCC
TARYKSISVENLEEGYAFLESLSAPYVLKADGLAAGKGVLIIDNLEEAKKELGDMLGGMF
CHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHC
GDASKTVVIEEFLDGIECSVFVLTDGNDYKVLPVAKDYKRIGEGNTGLNTGGMGAVSPVP
CCCCCEEEHHHHHCCCCEEEEEEECCCCEEEEECHHHHHHHCCCCCCCCCCCCCCCCCCC
FADEVFMAKVDERIIRPTVEGLKAEHIDYKGFIFLGLINVKNEPMVIEYNCRMGDPETEV
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCEE
VMLRIQSDLVDLLEGVAEGNLAGRTLVQDPRAAVTVMLVSGGYPEAYEKNKVITGLDGVS
EEEEEHHHHHHHHHHHHCCCCCCCEECCCCCCEEEEEEEECCCCCHHCCCCEEEECCCCC
SNNIVFHAGTKLVDGQILTNGGRVIAVSSYGATKEEALAQSFAGAKQIDFDKKYFRRDIG
CCCEEEECCCEEECCEEEECCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCC
FDL
CCC
>Mature Secondary Structure
MNILLLGSGGREHAIAWKIAQSPKVDKLFIAPGNAGTALVGTNVDIKADDFAAIKDFVLA
CEEEEEECCCCCCEEEEEECCCCCCCEEEEECCCCCCEEEECCEEECCCCHHHHHHHHHH
NQVNMVVVGPEDPLVKGVYDYFKKDEALSSIPVIGPSGEGAQLEGSKDFAKAFMMRHNIP
CCCCEEEECCCCCHHHHHHHHHHCCCHHHCCCEECCCCCCCEECCCHHHHHHHHHHCCCC
TARYKSISVENLEEGYAFLESLSAPYVLKADGLAAGKGVLIIDNLEEAKKELGDMLGGMF
CHHCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHC
GDASKTVVIEEFLDGIECSVFVLTDGNDYKVLPVAKDYKRIGEGNTGLNTGGMGAVSPVP
CCCCCEEEHHHHHCCCCEEEEEEECCCCEEEEECHHHHHHHCCCCCCCCCCCCCCCCCCC
FADEVFMAKVDERIIRPTVEGLKAEHIDYKGFIFLGLINVKNEPMVIEYNCRMGDPETEV
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCEE
VMLRIQSDLVDLLEGVAEGNLAGRTLVQDPRAAVTVMLVSGGYPEAYEKNKVITGLDGVS
EEEEEHHHHHHHHHHHHCCCCCCCEECCCCCCEEEEEEEECCCCCHHCCCCEEEECCCCC
SNNIVFHAGTKLVDGQILTNGGRVIAVSSYGATKEEALAQSFAGAKQIDFDKKYFRRDIG
CCCEEEECCCEEECCEEEECCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCC
FDL
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]