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The map label for this gene is rlmL [H]

Identifier: 150009678

GI number: 150009678

Start: 3708235

End: 3709785

Strand: Direct

Name: rlmL [H]

Synonym: BDI_3093

Alternate gene names: 150009678

Gene position: 3708235-3709785 (Clockwise)

Preceding gene: 150009677

Following gene: 150009679

Centisome position: 77.07

GC content: 47.13

Gene sequence:

>1551_bases
ATGAGCGAGACATTTGAAATGGTGGCCAAAACCCTATACGGGTTGGAAGAGATCTTGGCCGAGGAGTTATTAGCTTTAGG
TGCCAACGACTTGCAGATCGGTCGTCGTATGGTTTCCTTTACGGGTGATAAGGAATTGCTTTATAAAGCGAATTTCCACT
GCCGTACTGCCCTGCGCATTCTGAAACCGATTTATCATTTCAAGGCCAAAGATGCTGATACCGTTTATAAAGAGGTGAAA
AAGGTAGAGTGGGAGAAGTATTTGTCGCTGGACAAGACGTTCGCGATCGACTCGGTGATCTATTCCGAGGATTTCAATCA
CTCGAAGTTTGTCGCTTACCGTACGAAAGACGCTATCGTGGATTATTTCATCGAGAAGTTCAAGAAGCGTCCGTCCGTTC
GTGTCAATAACCCGGACTTATATATCAATATCCATATTTCGCATAACGATTGTACCTTATCTATCGATAGCTCCGGCGAG
TCTTTGCACAAGCGTGGTTATCGGGTGGATCAAACGGAAGCTCCGTTGAATGAGGTGTTGGCTGCCGGCATGATCCTAAA
AACCGGTTGGAAAGGAGAATCTAATTTCGTAGATCCGATGTGTGGTTCCGGTACATTGTTGATCGAGGCTGCCATGATCG
CCTTGAATATCGCTCCGGGTATCCATCGGAAAGAGTTCGCTTTCCAGAAATGGGTGGACTATGATGAGGAATTGTTCGAT
CGTATTTATAATGATGAGAGCGGGGAGCGTGAGTTCGCTTTCCATTGCTATGGCTCCGATATTTCCCAAGCCGCTATCGA
TATCGCCTTGGAGAATATCCGTAGCGCTGGCTTGATGAAATATATCGATCTGAAGGTGAAACCGTTCCAACAATATACAG
AGGCTCCGAAGCCAGGTATATTGGTGACGAACCCACCTTATGGAGAGCGTATATCATCTCGTGATCTATTGGGATTGTAT
AATATGATCGGCGAGCGTTTGAAACATGTGTTCATGGGATATAAGGCTTGGATTTTGAGCTATAAAGATGAATGTTTCGA
TAAGATCGGCTTGCGCCCGAGCGAGAAGATCAAATTGATGAACGGTTCCTTGGAGTGCGAATACCGTTGCTATGAGTTAT
TTGAGGGAACCAATAAGGACTTCAAAAAGGCATTGAATGAGGATGGAGAGGAGCGTCCGCGCAGATCGGAAGGCTCTTTC
GAGCGTAGAGGCAATGATCGTCCAAACTTCCGTACGGGTCGTGTGGATCGTCCGGAGCGTCGTTTCGCCGCTGCTCATAG
CGAGGATGATGAGGAGCGTTTCGCTTCCATTCCGGGGAAACGTATCTTTGGAGAGGATCGGGGAGAGGATCGTCCGGTCC
GCAAGAAATTCGTGGATGCCCCGGTTCGTAAGCCGATCAAGCCAAAAGGAGAACGTCCTGTGAAAATGCGTTATCGGGAT
GAGGATGACCGTAAGAAAGGGTTTGGCGATCATAAGCGGGATGGAAAGCGCCCGTTTAGCAAACCTATAAAGCCTATAAA
ACGGAGAGGGTATGATGATTATGAGGATTAA

Upstream 100 bases:

>100_bases
CGAAGCATGTCAGAGAGCGAAATGAGAATAATTAATAATAAACAACGTAATACGCAATTGGGAATAATTTTCAATTCTCA
ATTCTCAATTTTCAATTAAA

Downstream 100 bases:

>100_bases
ATCATCTTTATTCATAAGCCTATGTCTGATTTTATTAACGATGTCGATTACCGCCCAAGATAAGCAACTCTCCTTGCACG
ACTTGATTCCGGGAGGAAAA

Product: putative N6-adenine-specific DNA methylase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 516; Mature: 515

Protein sequence:

>516_residues
MSETFEMVAKTLYGLEEILAEELLALGANDLQIGRRMVSFTGDKELLYKANFHCRTALRILKPIYHFKAKDADTVYKEVK
KVEWEKYLSLDKTFAIDSVIYSEDFNHSKFVAYRTKDAIVDYFIEKFKKRPSVRVNNPDLYINIHISHNDCTLSIDSSGE
SLHKRGYRVDQTEAPLNEVLAAGMILKTGWKGESNFVDPMCGSGTLLIEAAMIALNIAPGIHRKEFAFQKWVDYDEELFD
RIYNDESGEREFAFHCYGSDISQAAIDIALENIRSAGLMKYIDLKVKPFQQYTEAPKPGILVTNPPYGERISSRDLLGLY
NMIGERLKHVFMGYKAWILSYKDECFDKIGLRPSEKIKLMNGSLECEYRCYELFEGTNKDFKKALNEDGEERPRRSEGSF
ERRGNDRPNFRTGRVDRPERRFAAAHSEDDEERFASIPGKRIFGEDRGEDRPVRKKFVDAPVRKPIKPKGERPVKMRYRD
EDDRKKGFGDHKRDGKRPFSKPIKPIKRRGYDDYED

Sequences:

>Translated_516_residues
MSETFEMVAKTLYGLEEILAEELLALGANDLQIGRRMVSFTGDKELLYKANFHCRTALRILKPIYHFKAKDADTVYKEVK
KVEWEKYLSLDKTFAIDSVIYSEDFNHSKFVAYRTKDAIVDYFIEKFKKRPSVRVNNPDLYINIHISHNDCTLSIDSSGE
SLHKRGYRVDQTEAPLNEVLAAGMILKTGWKGESNFVDPMCGSGTLLIEAAMIALNIAPGIHRKEFAFQKWVDYDEELFD
RIYNDESGEREFAFHCYGSDISQAAIDIALENIRSAGLMKYIDLKVKPFQQYTEAPKPGILVTNPPYGERISSRDLLGLY
NMIGERLKHVFMGYKAWILSYKDECFDKIGLRPSEKIKLMNGSLECEYRCYELFEGTNKDFKKALNEDGEERPRRSEGSF
ERRGNDRPNFRTGRVDRPERRFAAAHSEDDEERFASIPGKRIFGEDRGEDRPVRKKFVDAPVRKPIKPKGERPVKMRYRD
EDDRKKGFGDHKRDGKRPFSKPIKPIKRRGYDDYED
>Mature_515_residues
SETFEMVAKTLYGLEEILAEELLALGANDLQIGRRMVSFTGDKELLYKANFHCRTALRILKPIYHFKAKDADTVYKEVKK
VEWEKYLSLDKTFAIDSVIYSEDFNHSKFVAYRTKDAIVDYFIEKFKKRPSVRVNNPDLYINIHISHNDCTLSIDSSGES
LHKRGYRVDQTEAPLNEVLAAGMILKTGWKGESNFVDPMCGSGTLLIEAAMIALNIAPGIHRKEFAFQKWVDYDEELFDR
IYNDESGEREFAFHCYGSDISQAAIDIALENIRSAGLMKYIDLKVKPFQQYTEAPKPGILVTNPPYGERISSRDLLGLYN
MIGERLKHVFMGYKAWILSYKDECFDKIGLRPSEKIKLMNGSLECEYRCYELFEGTNKDFKKALNEDGEERPRRSEGSFE
RRGNDRPNFRTGRVDRPERRFAAAHSEDDEERFASIPGKRIFGEDRGEDRPVRKKFVDAPVRKPIKPKGERPVKMRYRDE
DDRKKGFGDHKRDGKRPFSKPIKPIKRRGYDDYED

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=407, Percent_Identity=34.6437346437346, Blast_Score=234, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 59839; Mature: 59707

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETFEMVAKTLYGLEEILAEELLALGANDLQIGRRMVSFTGDKELLYKANFHCRTALRI
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHH
LKPIYHFKAKDADTVYKEVKKVEWEKYLSLDKTFAIDSVIYSEDFNHSKFVAYRTKDAIV
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEECHHHHH
DYFIEKFKKRPSVRVNNPDLYINIHISHNDCTLSIDSSGESLHKRGYRVDQTEAPLNEVL
HHHHHHHHCCCCEEECCCEEEEEEEEECCCEEEEECCCCHHHHHCCCCCCCCCCCHHHHH
AAGMILKTGWKGESNFVDPMCGSGTLLIEAAMIALNIAPGIHRKEFAFQKWVDYDEELFD
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHH
RIYNDESGEREFAFHCYGSDISQAAIDIALENIRSAGLMKYIDLKVKPFQQYTEAPKPGI
HHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHCCCCCCE
LVTNPPYGERISSRDLLGLYNMIGERLKHVFMGYKAWILSYKDECFDKIGLRPSEKIKLM
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
NGSLECEYRCYELFEGTNKDFKKALNEDGEERPRRSEGSFERRGNDRPNFRTGRVDRPER
CCCCCCHHHHHHHHCCCCHHHHHHHCCCCHHCCCCCCCCHHHCCCCCCCCCCCCCCCHHH
RFAAAHSEDDEERFASIPGKRIFGEDRGEDRPVRKKFVDAPVRKPIKPKGERPVKMRYRD
HHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEECCC
EDDRKKGFGDHKRDGKRPFSKPIKPIKRRGYDDYED
CHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SETFEMVAKTLYGLEEILAEELLALGANDLQIGRRMVSFTGDKELLYKANFHCRTALRI
CHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHH
LKPIYHFKAKDADTVYKEVKKVEWEKYLSLDKTFAIDSVIYSEDFNHSKFVAYRTKDAIV
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEECHHHHH
DYFIEKFKKRPSVRVNNPDLYINIHISHNDCTLSIDSSGESLHKRGYRVDQTEAPLNEVL
HHHHHHHHCCCCEEECCCEEEEEEEEECCCEEEEECCCCHHHHHCCCCCCCCCCCHHHHH
AAGMILKTGWKGESNFVDPMCGSGTLLIEAAMIALNIAPGIHRKEFAFQKWVDYDEELFD
HHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHH
RIYNDESGEREFAFHCYGSDISQAAIDIALENIRSAGLMKYIDLKVKPFQQYTEAPKPGI
HHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHCCCCCCE
LVTNPPYGERISSRDLLGLYNMIGERLKHVFMGYKAWILSYKDECFDKIGLRPSEKIKLM
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
NGSLECEYRCYELFEGTNKDFKKALNEDGEERPRRSEGSFERRGNDRPNFRTGRVDRPER
CCCCCCHHHHHHHHCCCCHHHHHHHCCCCHHCCCCCCCCHHHCCCCCCCCCCCCCCCHHH
RFAAAHSEDDEERFASIPGKRIFGEDRGEDRPVRKKFVDAPVRKPIKPKGERPVKMRYRD
HHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEECCC
EDDRKKGFGDHKRDGKRPFSKPIKPIKRRGYDDYED
CHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA