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The map label for this gene is lpdA [C]

Identifier: 150009638

GI number: 150009638

Start: 3655804

End: 3657150

Strand: Direct

Name: lpdA [C]

Synonym: BDI_3052

Alternate gene names: 150009638

Gene position: 3655804-3657150 (Clockwise)

Preceding gene: 150009637

Following gene: 150009639

Centisome position: 75.98

GC content: 50.41

Gene sequence:

>1347_bases
ATGAAATACGATGTCGCTATAATCGGTGGAGGTCCTGCCGGTTATACTGCCGCAGAGAGAGCGGCGCAGGGTGGGCTTTC
CACGATTCTTTTTGAGAAAAACGCATTGGGCGGGGTTTGTTTGAATGAGGGATGCGTGCCGACAAAGACGCTTCTTTATT
CCGCTAAGACGTATGATAATATCAAGCATGCTTCCAAGTATGCGGTGAAAGCGGAGAATCCTTCTTTTGATCTCCCGAAA
ATCATCGCCCGTAAGAATAAGGTCGTGAAAAAGCTGACAGCCGGTATCCGTATGAAAATGACGGAGCATGGCGTGGTGAT
GGTTACGGCGGAAGCTTGTATCCAAGGGCGTGCGGCCGACGGGACGATTACGATCGCCGCCGGAGAAGAGCTATACGAGG
CTGCGAACCTTTTGATCTGTACGGGATCGGAAACGGTAATTCCTCCTATACCCGGATTGGCGGAGACGGAATATTGGACG
AGCCGGGAGGCGTTGCAATCGAAGGAGCTTCCGGCCTCTTTGGTGATAATCGGTGGAGGCGTGATCGGTATGGAGTTCGC
TTCTTTCTTCAATAGCCTTGGGGTAGAGGTACATGTCGTGGAGATGTTGGATAAGATATTAGGCCCGATGGATCGCGAGC
TGTCCGAAATGTTGCAAGCGGAATACGCTAAACGGGGGATTAAGTTCTATCTAAGTCATAAAGTGACCGGGGTGCATGGA
ACCGAGGTTTCTGTAGAGAAAGACGGGGAGACATTCACGTTACATGGCGATAAGGTCCTTTTGAGTGTAGGGCGTCGTCC
GGTTACGAAAGGTTTTGGCTTGGAGACACTGGCTCCGGAGACTTTTCGTAATGGGGTGAAGGTGAATGAGTACATGCAGA
CTTCCCTGCCAAATGTATATGCTTGTGGTGATATTACCGCTTTTTCCTTACTGGCGCATACGGCAGTTAGCGAGGCGGAG
GTGGCCGTGGATCATCTCTTGGGCAAATCGCGCCCGATGAGTTATAAGGCTATTCCGGGTGTCGTTTATACGAATCCGGA
GATCGCCGGTGTCGGGAAGACAGAGGAGGAGTTGCAGGCAGAAGGTATTTCGTATACCGTGAAAAAAATACCGATGGCAT
TCTCTGGGCGTTTTGTCGCGGAGAATGAGATGGGGAATGGTGTTTGTAAGTTGATCTTGTCGGAGGATGAAACGTTGATC
GGTGCCCATATGTTGGGTAATCCGGCCTCGGAGTTGATCGTGATCGCCGGAATCGCTATTGAGAAGGGGATGAAATCGGA
TGAGTTAAAGTCGTTTGTGTTCCCGCATCCTACGGTAGGAGAGATTATTAAGGAAGCGCTTTATTGA

Upstream 100 bases:

>100_bases
GGGAAACCTTGGATGCTCATATCTCCGACCAACCGGGCTGATTTTATCAAACAGTTGAGAAAGATAAATCCTAATATACA
ACTTAAATAGATCTAATAGT

Downstream 100 bases:

>100_bases
AATAATTATGTACCTTTGTGCCGATAATAGCGGCACATGGGTATATTTGAAGGATATGTAGGGATTCGTTTGTGGGACGG
ACAATTGGTGGACGATGTGG

Product: alpha keto acid dehydrogenase complex, E3 component, lipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MKYDVAIIGGGPAGYTAAERAAQGGLSTILFEKNALGGVCLNEGCVPTKTLLYSAKTYDNIKHASKYAVKAENPSFDLPK
IIARKNKVVKKLTAGIRMKMTEHGVVMVTAEACIQGRAADGTITIAAGEELYEAANLLICTGSETVIPPIPGLAETEYWT
SREALQSKELPASLVIIGGGVIGMEFASFFNSLGVEVHVVEMLDKILGPMDRELSEMLQAEYAKRGIKFYLSHKVTGVHG
TEVSVEKDGETFTLHGDKVLLSVGRRPVTKGFGLETLAPETFRNGVKVNEYMQTSLPNVYACGDITAFSLLAHTAVSEAE
VAVDHLLGKSRPMSYKAIPGVVYTNPEIAGVGKTEEELQAEGISYTVKKIPMAFSGRFVAENEMGNGVCKLILSEDETLI
GAHMLGNPASELIVIAGIAIEKGMKSDELKSFVFPHPTVGEIIKEALY

Sequences:

>Translated_448_residues
MKYDVAIIGGGPAGYTAAERAAQGGLSTILFEKNALGGVCLNEGCVPTKTLLYSAKTYDNIKHASKYAVKAENPSFDLPK
IIARKNKVVKKLTAGIRMKMTEHGVVMVTAEACIQGRAADGTITIAAGEELYEAANLLICTGSETVIPPIPGLAETEYWT
SREALQSKELPASLVIIGGGVIGMEFASFFNSLGVEVHVVEMLDKILGPMDRELSEMLQAEYAKRGIKFYLSHKVTGVHG
TEVSVEKDGETFTLHGDKVLLSVGRRPVTKGFGLETLAPETFRNGVKVNEYMQTSLPNVYACGDITAFSLLAHTAVSEAE
VAVDHLLGKSRPMSYKAIPGVVYTNPEIAGVGKTEEELQAEGISYTVKKIPMAFSGRFVAENEMGNGVCKLILSEDETLI
GAHMLGNPASELIVIAGIAIEKGMKSDELKSFVFPHPTVGEIIKEALY
>Mature_448_residues
MKYDVAIIGGGPAGYTAAERAAQGGLSTILFEKNALGGVCLNEGCVPTKTLLYSAKTYDNIKHASKYAVKAENPSFDLPK
IIARKNKVVKKLTAGIRMKMTEHGVVMVTAEACIQGRAADGTITIAAGEELYEAANLLICTGSETVIPPIPGLAETEYWT
SREALQSKELPASLVIIGGGVIGMEFASFFNSLGVEVHVVEMLDKILGPMDRELSEMLQAEYAKRGIKFYLSHKVTGVHG
TEVSVEKDGETFTLHGDKVLLSVGRRPVTKGFGLETLAPETFRNGVKVNEYMQTSLPNVYACGDITAFSLLAHTAVSEAE
VAVDHLLGKSRPMSYKAIPGVVYTNPEIAGVGKTEEELQAEGISYTVKKIPMAFSGRFVAENEMGNGVCKLILSEDETLI
GAHMLGNPASELIVIAGIAIEKGMKSDELKSFVFPHPTVGEIIKEALY

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=459, Percent_Identity=37.6906318082789, Blast_Score=274, Evalue=1e-73,
Organism=Homo sapiens, GI50301238, Length=458, Percent_Identity=28.1659388646288, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI33519430, Length=480, Percent_Identity=26.25, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI33519428, Length=480, Percent_Identity=26.25, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI33519426, Length=480, Percent_Identity=26.25, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI148277065, Length=480, Percent_Identity=26.25, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI148277071, Length=480, Percent_Identity=26.25, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI22035672, Length=433, Percent_Identity=25.8660508083141, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI291045266, Length=434, Percent_Identity=25.8064516129032, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI291045268, Length=428, Percent_Identity=24.5327102803738, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1786307, Length=454, Percent_Identity=33.7004405286344, Blast_Score=244, Evalue=6e-66,
Organism=Escherichia coli, GI87081717, Length=461, Percent_Identity=30.1518438177874, Blast_Score=162, Evalue=5e-41,
Organism=Escherichia coli, GI87082354, Length=461, Percent_Identity=26.6811279826464, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1789915, Length=435, Percent_Identity=25.5172413793103, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1789065, Length=221, Percent_Identity=27.6018099547511, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1789765, Length=351, Percent_Identity=28.2051282051282, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=462, Percent_Identity=37.2294372294372, Blast_Score=284, Evalue=8e-77,
Organism=Caenorhabditis elegans, GI71983419, Length=442, Percent_Identity=29.4117647058824, Blast_Score=137, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71983429, Length=442, Percent_Identity=29.4117647058824, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17557007, Length=474, Percent_Identity=25.7383966244726, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=439, Percent_Identity=24.373576309795, Blast_Score=96, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6321091, Length=464, Percent_Identity=35.1293103448276, Blast_Score=229, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6325240, Length=466, Percent_Identity=28.3261802575107, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6325166, Length=464, Percent_Identity=26.7241379310345, Blast_Score=167, Evalue=5e-42,
Organism=Drosophila melanogaster, GI21358499, Length=463, Percent_Identity=37.5809935205184, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI17737741, Length=470, Percent_Identity=24.8936170212766, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24640549, Length=476, Percent_Identity=25.4201680672269, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24640553, Length=476, Percent_Identity=25.4201680672269, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24640551, Length=476, Percent_Identity=25.4201680672269, Blast_Score=112, Evalue=7e-25,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 48023; Mature: 48023

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYDVAIIGGGPAGYTAAERAAQGGLSTILFEKNALGGVCLNEGCVPTKTLLYSAKTYDN
CCEEEEEECCCCCCCHHHHHHHHCCCEEEEEECCCCCCEEECCCCCCCHHHEEEHHHHHH
IKHASKYAVKAENPSFDLPKIIARKNKVVKKLTAGIRMKMTEHGVVMVTAEACIQGRAAD
HHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEHHHHHCCCCCC
GTITIAAGEELYEAANLLICTGSETVIPPIPGLAETEYWTSREALQSKELPASLVIIGGG
CEEEEECCHHHHHCCCEEEECCCCEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECC
VIGMEFASFFNSLGVEVHVVEMLDKILGPMDRELSEMLQAEYAKRGIKFYLSHKVTGVHG
HHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEEEEEEEECCC
TEVSVEKDGETFTLHGDKVLLSVGRRPVTKGFGLETLAPETFRNGVKVNEYMQTSLPNVY
CEEEEECCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCHHHHCCCEEHHHHHHCCCCEE
ACGDITAFSLLAHTAVSEAEVAVDHLLGKSRPMSYKAIPGVVYTNPEIAGVGKTEEELQA
ECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCEEECCCCEEECCCCHHHHHH
EGISYTVKKIPMAFSGRFVAENEMGNGVCKLILSEDETLIGAHMLGNPASELIVIAGIAI
CCCEEEHHHCCHHHCCCEEECCCCCCCEEEEEECCCCEEEEHHHCCCCCCCEEEEEEEHH
EKGMKSDELKSFVFPHPTVGEIIKEALY
HCCCCHHHHHHHCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKYDVAIIGGGPAGYTAAERAAQGGLSTILFEKNALGGVCLNEGCVPTKTLLYSAKTYDN
CCEEEEEECCCCCCCHHHHHHHHCCCEEEEEECCCCCCEEECCCCCCCHHHEEEHHHHHH
IKHASKYAVKAENPSFDLPKIIARKNKVVKKLTAGIRMKMTEHGVVMVTAEACIQGRAAD
HHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEHHHHHCCCCCC
GTITIAAGEELYEAANLLICTGSETVIPPIPGLAETEYWTSREALQSKELPASLVIIGGG
CEEEEECCHHHHHCCCEEEECCCCEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECC
VIGMEFASFFNSLGVEVHVVEMLDKILGPMDRELSEMLQAEYAKRGIKFYLSHKVTGVHG
HHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEEEEEEEECCC
TEVSVEKDGETFTLHGDKVLLSVGRRPVTKGFGLETLAPETFRNGVKVNEYMQTSLPNVY
CEEEEECCCCEEEEECCEEEEECCCCCCCCCCCCCCCCCHHHHCCCEEHHHHHHCCCCEE
ACGDITAFSLLAHTAVSEAEVAVDHLLGKSRPMSYKAIPGVVYTNPEIAGVGKTEEELQA
ECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCEEECCCCEEECCCCHHHHHH
EGISYTVKKIPMAFSGRFVAENEMGNGVCKLILSEDETLIGAHMLGNPASELIVIAGIAI
CCCEEEHHHCCHHHCCCEEECCCCCCCEEEEEECCCCEEEEHHHCCCCCCCEEEEEEEHH
EKGMKSDELKSFVFPHPTVGEIIKEALY
HCCCCHHHHHHHCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2832161; 2404760; 1880807 [H]