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The map label for this gene is mepA [H]

Identifier: 150009623

GI number: 150009623

Start: 3636512

End: 3637888

Strand: Reverse

Name: mepA [H]

Synonym: BDI_3037

Alternate gene names: 150009623

Gene position: 3637888-3636512 (Counterclockwise)

Preceding gene: 150009655

Following gene: 150009621

Centisome position: 75.61

GC content: 46.41

Gene sequence:

>1377_bases
ATGAAGAACAAATACGAGGCTAGGCTAGGTACGGAACGTATGCTACCGCTGGTTTTCAAAATGGCGCTTCCGGCGGTAGC
GGCCCAATTTGTCAACTTACTTTATAATATCGTAGATCGTATCTATATCGGGCATATTCCCGAGATCGGGACAAACGCTT
TGGCCGGTATAGGGGTGACCACCTCCATCGTGATCTTGATCTCTTCTTTCTCCGCTATCGTGGGAGCGGGCGGTTCGCCG
TTAGCGGCTATCGCTCTCGGACAGGGCGACCGGGAACGGGCCGGAAAAATAGTGGGAAATGGATTTATATTGCTTATCCT
ATTTACTATATTCACTTCCCTCATCACCTATACCTTCATGGAACCGATCCTGCTTTTTACAGGAGCATCAAAATATACGT
TAGGATATGCGGTGGATTATCTCTCTATTTATTTACTGGGCACTCTGTTTGTCGAGATATCCACGGGCTTGAATGCCTTT
ATCAACGCACAAGGGCGTCCGTCTATCGCTATGTGCTCGGTTTTGATCGGGGCTTTATTGAATATCGTATTAGACCCTAT
CTTCATTTTCTGGTTGGATATGGGGATAAAAGGCGCCGCGCTCGCCACGATCCTATCGCAAGCTTGCAGTGCCATATGGG
TATTGAGCTTCCTTTTCTCAAGACGTGCCTCCTTGCCTTTGGAAAGGCGGTATATGAGACTGGACCGAAGAATCGTACTC
TCGATGTTCGCTTTGGGAATTTCTCCGTTCATCATGGCCAGCACGGAGAGTCTGGTGGGTTTCGTCTTGAATAGTAGTTT
GAAAAATTTTGGTGATATCTATGTGAGCGCATTGGCTATCTTACAGAGTGCCATGCAATTCGCTAGCGTACCGCTGACCG
GTTTCGCCCAAGGATTCGTACCGATCATAAGCTATAATTACGGACATGGAGACAAACAGCGGGTGAAAGATTGTTTTCGT
ATCGTGCTAATCGTTATGTTCTCTTTCAACTTGATACTCATGCTATTCATGATCTTATTCCCCTCAACCGTAGCCTCCGC
TTTTACAAGCGACGAGGAGTTGATCGATACCGTCCGTTGGGTCATGCCCATCTTCTTGGCGGGTATGACGATTTTTGGCT
TACAACGAGCTTGCCAGAATATGTTCGTAGCGTTGGGACAAGCTAAGATATCTATCTTTATCGCCTTGCTTCGTAAAGTA
ATCCTCTTGATTCCTTTGGCATTACTATTACCTCATTTTATGGGGGTAACCGGCGTATATGCGGCAGAAGCCGTATCGGA
TGCCACGGCCGCTATTTGCTGTACGTTGTTATTTATATGGAAATTCCCGAAGATACTAGGGAAAATCAAGCAAAAAGAAG
AAAGGGCTGCCCAATAA

Upstream 100 bases:

>100_bases
TTAAGTGTTTTTTATTAACTGGTAATTTATGGAACTCGATTATATAATCCAACAAGTTCTTGTAACTTTGCAGCCATTTT
AATTGAAATCCCGATTGAAT

Downstream 100 bases:

>100_bases
CGGACAGCCCCCTGATAAAGCCTTAAAGATTAAAATGAATCGTATTAATATCCCGGATTCTGTGTCAAGTTAGGATTCAG
GCTAAGGATATCGGCATAGA

Product: cation efflux pump

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MKNKYEARLGTERMLPLVFKMALPAVAAQFVNLLYNIVDRIYIGHIPEIGTNALAGIGVTTSIVILISSFSAIVGAGGSP
LAAIALGQGDRERAGKIVGNGFILLILFTIFTSLITYTFMEPILLFTGASKYTLGYAVDYLSIYLLGTLFVEISTGLNAF
INAQGRPSIAMCSVLIGALLNIVLDPIFIFWLDMGIKGAALATILSQACSAIWVLSFLFSRRASLPLERRYMRLDRRIVL
SMFALGISPFIMASTESLVGFVLNSSLKNFGDIYVSALAILQSAMQFASVPLTGFAQGFVPIISYNYGHGDKQRVKDCFR
IVLIVMFSFNLILMLFMILFPSTVASAFTSDEELIDTVRWVMPIFLAGMTIFGLQRACQNMFVALGQAKISIFIALLRKV
ILLIPLALLLPHFMGVTGVYAAEAVSDATAAICCTLLFIWKFPKILGKIKQKEERAAQ

Sequences:

>Translated_458_residues
MKNKYEARLGTERMLPLVFKMALPAVAAQFVNLLYNIVDRIYIGHIPEIGTNALAGIGVTTSIVILISSFSAIVGAGGSP
LAAIALGQGDRERAGKIVGNGFILLILFTIFTSLITYTFMEPILLFTGASKYTLGYAVDYLSIYLLGTLFVEISTGLNAF
INAQGRPSIAMCSVLIGALLNIVLDPIFIFWLDMGIKGAALATILSQACSAIWVLSFLFSRRASLPLERRYMRLDRRIVL
SMFALGISPFIMASTESLVGFVLNSSLKNFGDIYVSALAILQSAMQFASVPLTGFAQGFVPIISYNYGHGDKQRVKDCFR
IVLIVMFSFNLILMLFMILFPSTVASAFTSDEELIDTVRWVMPIFLAGMTIFGLQRACQNMFVALGQAKISIFIALLRKV
ILLIPLALLLPHFMGVTGVYAAEAVSDATAAICCTLLFIWKFPKILGKIKQKEERAAQ
>Mature_458_residues
MKNKYEARLGTERMLPLVFKMALPAVAAQFVNLLYNIVDRIYIGHIPEIGTNALAGIGVTTSIVILISSFSAIVGAGGSP
LAAIALGQGDRERAGKIVGNGFILLILFTIFTSLITYTFMEPILLFTGASKYTLGYAVDYLSIYLLGTLFVEISTGLNAF
INAQGRPSIAMCSVLIGALLNIVLDPIFIFWLDMGIKGAALATILSQACSAIWVLSFLFSRRASLPLERRYMRLDRRIVL
SMFALGISPFIMASTESLVGFVLNSSLKNFGDIYVSALAILQSAMQFASVPLTGFAQGFVPIISYNYGHGDKQRVKDCFR
IVLIVMFSFNLILMLFMILFPSTVASAFTSDEELIDTVRWVMPIFLAGMTIFGLQRACQNMFVALGQAKISIFIALLRKV
ILLIPLALLLPHFMGVTGVYAAEAVSDATAAICCTLLFIWKFPKILGKIKQKEERAAQ

Specific function: Multidrug resistance efflux protein [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MepA subfamily [H]

Homologues:

Organism=Escherichia coli, GI226510954, Length=442, Percent_Identity=23.5294117647059, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528
- InterPro:   IPR015522 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 50054; Mature: 50054

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKYEARLGTERMLPLVFKMALPAVAAQFVNLLYNIVDRIYIGHIPEIGTNALAGIGVT
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHH
TSIVILISSFSAIVGAGGSPLAAIALGQGDRERAGKIVGNGFILLILFTIFTSLITYTFM
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCHHHCCHHHHHHHHHHHHHHHHHHHH
EPILLFTGASKYTLGYAVDYLSIYLLGTLFVEISTGLNAFINAQGRPSIAMCSVLIGALL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHH
NIVLDPIFIFWLDMGIKGAALATILSQACSAIWVLSFLFSRRASLPLERRYMRLDRRIVL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
SMFALGISPFIMASTESLVGFVLNSSLKNFGDIYVSALAILQSAMQFASVPLTGFAQGFV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCH
PIISYNYGHGDKQRVKDCFRIVLIVMFSFNLILMLFMILFPSTVASAFTSDEELIDTVRW
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VMPIFLAGMTIFGLQRACQNMFVALGQAKISIFIALLRKVILLIPLALLLPHFMGVTGVY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAEAVSDATAAICCTLLFIWKFPKILGKIKQKEERAAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKNKYEARLGTERMLPLVFKMALPAVAAQFVNLLYNIVDRIYIGHIPEIGTNALAGIGVT
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHH
TSIVILISSFSAIVGAGGSPLAAIALGQGDRERAGKIVGNGFILLILFTIFTSLITYTFM
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCHHHCCHHHHHHHHHHHHHHHHHHHH
EPILLFTGASKYTLGYAVDYLSIYLLGTLFVEISTGLNAFINAQGRPSIAMCSVLIGALL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHH
NIVLDPIFIFWLDMGIKGAALATILSQACSAIWVLSFLFSRRASLPLERRYMRLDRRIVL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
SMFALGISPFIMASTESLVGFVLNSSLKNFGDIYVSALAILQSAMQFASVPLTGFAQGFV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCH
PIISYNYGHGDKQRVKDCFRIVLIVMFSFNLILMLFMILFPSTVASAFTSDEELIDTVRW
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VMPIFLAGMTIFGLQRACQNMFVALGQAKISIFIALLRKVILLIPLALLLPHFMGVTGVY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAEAVSDATAAICCTLLFIWKFPKILGKIKQKEERAAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA