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The map label for this gene is tvaII [H]

Identifier: 150009339

GI number: 150009339

Start: 3270167

End: 3272008

Strand: Reverse

Name: tvaII [H]

Synonym: BDI_2746

Alternate gene names: 150009339

Gene position: 3272008-3270167 (Counterclockwise)

Preceding gene: 150009340

Following gene: 150009338

Centisome position: 68.01

GC content: 37.95

Gene sequence:

>1842_bases
ATGAAAAGACTATTATTGTTTACCATTTGCTTGTTGTGCGCCAGTTCTGTATATGCTATTGATATTTCACGGTTAGAACC
TGCTTGCTGGTGGGTAGGGATGAAAAATCCGGAATTACAGATTATGGTATATGGCAAGAATATCGCAAGCTCTCAAGTTT
ATATTGATTATCCGGGGGTAAGAGTCAAGGAAATTGTTGGAGTCGAGAACCCTAATTACTTGTTCCTTTATTTGGATATC
TCCAGAGATGCCTCTCCCGGTACAATGAATTTGATATTTCAAAAAGGGAAAGAGAAAGAAATCCGGGCCTTTGAGTTAAA
GGAAAGAAATAAAAAGGTTGGAGCTGCTGGATTTTCACCTGCGGATGTGATGTACTTGATTACTCCAGATCGTTTTGCAA
ATGCGGATCCATCTAATGACAATCTAGATGATGTGAAAATAGATCGTTCGAGAGGGGGAGCTCGCCATGGAGGTGATTTA
CGTGGTATTATTAATAAATTGGATTATATCCGTGATTTAGGCTTTACGACAATTTGGTTAAATCCTGTATTGGAAAATAG
GATGCCGGGAGGTTCTTATCACGGATACGCTATTACTGATTTTTATCAGGTAGATCCTCGTTTTGGAACGAATGAAGAAT
ATTGTGAATTGATAGATAAGGCCCACGAAAAAGGAATGAAAGTCGTGATGGATATGATTTTTAATCATTGCGGAAGCTCT
CATTGGTGGTTAAAAGATTTTCCTTCCTCGGATTGGCTGAATAATCAAGACAACTTTGTTCAAACAAATCATTTTAAATG
GACTCTTATGGATGTTCACGCTCCTCAGTCGGAGAGAGAGATCTTGGTAAATGGTTGGTTTGGTAGAGGTATGCCAGATT
TAAATCAGAAAAATAGGCACTTAGCCCGATATTTGATTCAAAATAGCATTTGGTGGATTGAATACTCACGGATTGATGGT
ATCCGTCAAGATACACATCCATATGCAGATTATGATTTTATGGCAACTTGGTGTAAGGAGGTGGAAAATGAATATCCGGA
TTTCAATATTGTGGGAGAAGCTTGGTATCCAAGAGGGACTGCTTCGGCTTGGTGGCAAAGAGATTCTAAAATGAATGAAT
CTAATAGTAACTTAAAGACAGTGATGGATTTTGATTTGACCTTTACTTGCCAAAAAGCGTTTGGAGATGCATGTTCTAGC
CGTGAAGGATTCGAAGCAGGGTTATTTAAAATTTATGAAGTTATCGCACAGGATTTTTTATTCCCGGATCCTAATAATGT
ACTAGTCTTTTTGGATAATCATGATTTAGGACGCTTTATGCAGAAAGGAGAATCTGATTTAAGAAGGTATAAACAGGCTA
TTGCTTTTTTATTAACAACTCGAGGGATTCCTCAGATTTATTATGGGACAGAGATTTTAATGTCTGGGACAAAAGCAGAA
GGAGATGGTATTATCCGCACTGATTTTCCCGGAGGTTGGGCAGGAGATCCAAAAGATGCTTTCACTCGTGAGGGACGTAC
GGATTTGCAGAATCAAGCTTGGGATTATATGAGAAAATTATTGAATTGGAGACAGCGATGTGATGCTGTTAAAGAAGGTA
AGTTGATTCATTATACTCCTGATAAATCGGGTTGTTACGTATATGCTAGGGTAAAGGATAATAAAACGGTACTTGTTTTA
ATGAATGGAACAGATCAAAACCAGACATTAGATATGAATCGTTTTCATGAAGTCGTAAAAGATTATAGATCTGGGAAAGA
TATTATAACAGATACGATCGTTGCGATAGACACGGCGGTTCATATTCCGGCTCGTGGTGTTTATATTTTAGAACTTGTCT
AA

Upstream 100 bases:

>100_bases
CTCAAACAAGGAAATTTGTGGACAATCCTAAAGCAGATGCATACTTGAACAAAACATATAGAAGCCCTTGGCAATTTCCG
AATATAAAATGAATGAAATT

Downstream 100 bases:

>100_bases
CTTTAAATTTAATATGTCATGAGATGTAAAAGTATTAGTGAATTTATTATGTGCTTTATGCTTTCTTTGTTTTCATTGAC
AGCGTTTGCGCAAATCAAGA

Product: glycoside hydrolase family alpha-glucosidase

Products: NA

Alternate protein names: Alpha-amylase II; TVA II [H]

Number of amino acids: Translated: 613; Mature: 613

Protein sequence:

>613_residues
MKRLLLFTICLLCASSVYAIDISRLEPACWWVGMKNPELQIMVYGKNIASSQVYIDYPGVRVKEIVGVENPNYLFLYLDI
SRDASPGTMNLIFQKGKEKEIRAFELKERNKKVGAAGFSPADVMYLITPDRFANADPSNDNLDDVKIDRSRGGARHGGDL
RGIINKLDYIRDLGFTTIWLNPVLENRMPGGSYHGYAITDFYQVDPRFGTNEEYCELIDKAHEKGMKVVMDMIFNHCGSS
HWWLKDFPSSDWLNNQDNFVQTNHFKWTLMDVHAPQSEREILVNGWFGRGMPDLNQKNRHLARYLIQNSIWWIEYSRIDG
IRQDTHPYADYDFMATWCKEVENEYPDFNIVGEAWYPRGTASAWWQRDSKMNESNSNLKTVMDFDLTFTCQKAFGDACSS
REGFEAGLFKIYEVIAQDFLFPDPNNVLVFLDNHDLGRFMQKGESDLRRYKQAIAFLLTTRGIPQIYYGTEILMSGTKAE
GDGIIRTDFPGGWAGDPKDAFTREGRTDLQNQAWDYMRKLLNWRQRCDAVKEGKLIHYTPDKSGCYVYARVKDNKTVLVL
MNGTDQNQTLDMNRFHEVVKDYRSGKDIITDTIVAIDTAVHIPARGVYILELV

Sequences:

>Translated_613_residues
MKRLLLFTICLLCASSVYAIDISRLEPACWWVGMKNPELQIMVYGKNIASSQVYIDYPGVRVKEIVGVENPNYLFLYLDI
SRDASPGTMNLIFQKGKEKEIRAFELKERNKKVGAAGFSPADVMYLITPDRFANADPSNDNLDDVKIDRSRGGARHGGDL
RGIINKLDYIRDLGFTTIWLNPVLENRMPGGSYHGYAITDFYQVDPRFGTNEEYCELIDKAHEKGMKVVMDMIFNHCGSS
HWWLKDFPSSDWLNNQDNFVQTNHFKWTLMDVHAPQSEREILVNGWFGRGMPDLNQKNRHLARYLIQNSIWWIEYSRIDG
IRQDTHPYADYDFMATWCKEVENEYPDFNIVGEAWYPRGTASAWWQRDSKMNESNSNLKTVMDFDLTFTCQKAFGDACSS
REGFEAGLFKIYEVIAQDFLFPDPNNVLVFLDNHDLGRFMQKGESDLRRYKQAIAFLLTTRGIPQIYYGTEILMSGTKAE
GDGIIRTDFPGGWAGDPKDAFTREGRTDLQNQAWDYMRKLLNWRQRCDAVKEGKLIHYTPDKSGCYVYARVKDNKTVLVL
MNGTDQNQTLDMNRFHEVVKDYRSGKDIITDTIVAIDTAVHIPARGVYILELV
>Mature_613_residues
MKRLLLFTICLLCASSVYAIDISRLEPACWWVGMKNPELQIMVYGKNIASSQVYIDYPGVRVKEIVGVENPNYLFLYLDI
SRDASPGTMNLIFQKGKEKEIRAFELKERNKKVGAAGFSPADVMYLITPDRFANADPSNDNLDDVKIDRSRGGARHGGDL
RGIINKLDYIRDLGFTTIWLNPVLENRMPGGSYHGYAITDFYQVDPRFGTNEEYCELIDKAHEKGMKVVMDMIFNHCGSS
HWWLKDFPSSDWLNNQDNFVQTNHFKWTLMDVHAPQSEREILVNGWFGRGMPDLNQKNRHLARYLIQNSIWWIEYSRIDG
IRQDTHPYADYDFMATWCKEVENEYPDFNIVGEAWYPRGTASAWWQRDSKMNESNSNLKTVMDFDLTFTCQKAFGDACSS
REGFEAGLFKIYEVIAQDFLFPDPNNVLVFLDNHDLGRFMQKGESDLRRYKQAIAFLLTTRGIPQIYYGTEILMSGTKAE
GDGIIRTDFPGGWAGDPKDAFTREGRTDLQNQAWDYMRKLLNWRQRCDAVKEGKLIHYTPDKSGCYVYARVKDNKTVLVL
MNGTDQNQTLDMNRFHEVVKDYRSGKDIITDTIVAIDTAVHIPARGVYILELV

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=88, Percent_Identity=38.6363636363636, Blast_Score=74, Evalue=6e-13,
Organism=Escherichia coli, GI1786604, Length=518, Percent_Identity=24.7104247104247, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789995, Length=128, Percent_Identity=35.15625, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1790687, Length=89, Percent_Identity=35.9550561797753, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI32565753, Length=88, Percent_Identity=37.5, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322245, Length=371, Percent_Identity=25.0673854447439, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6321731, Length=190, Percent_Identity=31.5789473684211, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6319776, Length=190, Percent_Identity=31.5789473684211, Blast_Score=91, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6321726, Length=189, Percent_Identity=29.6296296296296, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324416, Length=189, Percent_Identity=29.1005291005291, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6322241, Length=189, Percent_Identity=29.1005291005291, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6322021, Length=189, Percent_Identity=29.1005291005291, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI221330053, Length=225, Percent_Identity=26.6666666666667, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI45549022, Length=94, Percent_Identity=40.4255319148936, Blast_Score=86, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24583749, Length=183, Percent_Identity=30.6010928961749, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24583747, Length=183, Percent_Identity=30.6010928961749, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24586599, Length=89, Percent_Identity=42.6966292134831, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24583745, Length=193, Percent_Identity=28.4974093264249, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24586587, Length=88, Percent_Identity=38.6363636363636, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24586597, Length=87, Percent_Identity=37.9310344827586, Blast_Score=77, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24586591, Length=89, Percent_Identity=39.3258426966292, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24586589, Length=215, Percent_Identity=25.1162790697674, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 70634; Mature: 70634

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLLLFTICLLCASSVYAIDISRLEPACWWVGMKNPELQIMVYGKNIASSQVYIDYPGV
CCHHHHHHHHHHHCCCEEEEEHHHCCCEEEEEECCCCCEEEEEEECCCCCCEEEEECCCC
RVKEIVGVENPNYLFLYLDISRDASPGTMNLIFQKGKEKEIRAFELKERNKKVGAAGFSP
EEEHEECCCCCCEEEEEEEECCCCCCCEEEEEEECCCCCCHHEEHHHHHCCCCCCCCCCC
ADVMYLITPDRFANADPSNDNLDDVKIDRSRGGARHGGDLRGIINKLDYIRDLGFTTIWL
CCEEEEECCHHHCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEE
NPVLENRMPGGSYHGYAITDFYQVDPRFGTNEEYCELIDKAHEKGMKVVMDMIFNHCGSS
CHHHHCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HWWLKDFPSSDWLNNQDNFVQTNHFKWTLMDVHAPQSEREILVNGWFGRGMPDLNQKNRH
CCEEECCCCCCCCCCCCCEEEECCEEEEEEEEECCCCCCEEEEEECCCCCCCCCCHHHHH
LARYLIQNSIWWIEYSRIDGIRQDTHPYADYDFMATWCKEVENEYPDFNIVGEAWYPRGT
HHHHHHHCCEEEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
ASAWWQRDSKMNESNSNLKTVMDFDLTFTCQKAFGDACSSREGFEAGLFKIYEVIAQDFL
CHHHHHCCCCCCCCCCCEEEEEEECEEEEEHHHHCCHHCCCCCHHHHHHHHHHHHHHHHC
FPDPNNVLVFLDNHDLGRFMQKGESDLRRYKQAIAFLLTTRGIPQIYYGTEILMSGTKAE
CCCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEECCCCC
GDGIIRTDFPGGWAGDPKDAFTREGRTDLQNQAWDYMRKLLNWRQRCDAVKEGKLIHYTP
CCCEEEECCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEECC
DKSGCYVYARVKDNKTVLVLMNGTDQNQTLDMNRFHEVVKDYRSGKDIITDTIVAIDTAV
CCCCEEEEEEEECCCEEEEEECCCCCCCEECHHHHHHHHHHHHCCCHHHHHHHHHHEEEE
HIPARGVYILELV
ECCCCCEEEEEEC
>Mature Secondary Structure
MKRLLLFTICLLCASSVYAIDISRLEPACWWVGMKNPELQIMVYGKNIASSQVYIDYPGV
CCHHHHHHHHHHHCCCEEEEEHHHCCCEEEEEECCCCCEEEEEEECCCCCCEEEEECCCC
RVKEIVGVENPNYLFLYLDISRDASPGTMNLIFQKGKEKEIRAFELKERNKKVGAAGFSP
EEEHEECCCCCCEEEEEEEECCCCCCCEEEEEEECCCCCCHHEEHHHHHCCCCCCCCCCC
ADVMYLITPDRFANADPSNDNLDDVKIDRSRGGARHGGDLRGIINKLDYIRDLGFTTIWL
CCEEEEECCHHHCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEE
NPVLENRMPGGSYHGYAITDFYQVDPRFGTNEEYCELIDKAHEKGMKVVMDMIFNHCGSS
CHHHHCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HWWLKDFPSSDWLNNQDNFVQTNHFKWTLMDVHAPQSEREILVNGWFGRGMPDLNQKNRH
CCEEECCCCCCCCCCCCCEEEECCEEEEEEEEECCCCCCEEEEEECCCCCCCCCCHHHHH
LARYLIQNSIWWIEYSRIDGIRQDTHPYADYDFMATWCKEVENEYPDFNIVGEAWYPRGT
HHHHHHHCCEEEEEEHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
ASAWWQRDSKMNESNSNLKTVMDFDLTFTCQKAFGDACSSREGFEAGLFKIYEVIAQDFL
CHHHHHCCCCCCCCCCCEEEEEEECEEEEEHHHHCCHHCCCCCHHHHHHHHHHHHHHHHC
FPDPNNVLVFLDNHDLGRFMQKGESDLRRYKQAIAFLLTTRGIPQIYYGTEILMSGTKAE
CCCCCCEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCEEEEECCCCC
GDGIIRTDFPGGWAGDPKDAFTREGRTDLQNQAWDYMRKLLNWRQRCDAVKEGKLIHYTP
CCCEEEECCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEECC
DKSGCYVYARVKDNKTVLVLMNGTDQNQTLDMNRFHEVVKDYRSGKDIITDTIVAIDTAV
CCCCEEEEEEEECCCEEEEEECCCCCCCEECHHHHHHHHHHHHCCCHHHHHHHHHHEEEE
HIPARGVYILELV
ECCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7763540; 10222200; 12051850 [H]