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The map label for this gene is nplT [H]

Identifier: 150009336

GI number: 150009336

Start: 3263775

End: 3265613

Strand: Reverse

Name: nplT [H]

Synonym: BDI_2743

Alternate gene names: 150009336

Gene position: 3265613-3263775 (Counterclockwise)

Preceding gene: 150009337

Following gene: 150009335

Centisome position: 67.87

GC content: 36.76

Gene sequence:

>1839_bases
ATGATTAGAAAAATAATAATATTTACTTCATTTCTGTTATTTGTACTTGGGACAGTGGCTGCTCAAACGATAAATAAGGT
AGAGCCGTTGTTTTGGTGGGCAGGAATGAGAAATCCAGAATTACAGTTGATGGTATATGGGGATCATATTTCTGAATATC
ATCCTGTTATAAATGAACCTGGTGTTTATTTAAAAAGTTGTGTAACGGTAGATAGTCCCAATTATTTAATATTATATTTA
GATTTATCACAAGCTCATTCTGGAACATTTGATATAACTTTTGTGAATGGGGAAAAGAAGTTCGTATATGCTTACGAGCT
GAAAGAGCGCAAGGAGAGTACAGATGATATACAAGGATATGATTCTTCGGATGTGTTGTATTTAATTATGCCGGACCGTT
TTGCTAATGGTGACCAATCGAATGATCAGATTTCTATGTCAACGGAATATGTGATGGATCGTAGTAAGCCTGGGGCTCGA
CATGGAGGTGATTTGAAGGGGATCGAAGATCATTTGGATTATTTTGTGGACCTTGGGGTAACAGCTATATGGTTAAATCC
CGTGTTGGAGAATGATGGGAAAGGAGGTTCTTATCATGGCTATTTCTCAACGGATATGTTTCATGTGGATAGGCGTCTAG
GATCAAATGAAGATTACCTTCGATTGATAAATAAAGCGCATCAAAAGGGATTACGGGTTGTTATGGATATGATTTTTAAT
CATATTGGAGCAAATCATCCTTGGGTGCTAGATGTGCCATCTAAGGATTGGTTTAATGTAAGTCCTTCTAAAAGGGGGAA
TCATGCTAAGGCTATTTTCTATGACAATTATGCATCAACTTATGATTCTGAAGTAATGAAATATGCAGGATTTGGAATAG
ATTTGAATCAAGCAAATCCTCATGTTGGTAAATATCTTATTCAGAATTCTATATGGTGGATTGAGTATGCTCGGATTGAT
GCGATTAGGCAAGATACATATCCATATTCCGATTTTGATATGATGCGTGAGTGGAATATCCAAATCATGAATGAATATCC
TCAGTTTAATATAGTTGGTGAGATTTGGACTAATTATACGATTGGTACGGCTTGGTGGCAAAAAGGGTGTAAACTTAATT
TTGGAAAAGATACGGAACTGAAGTCTGTGATGGATTTTACTCTGATGAGTATTGCAAGTGATATCTTTAATGACAAGTCT
TCACCGGAAGGGCGATTAGATAAAATCTATGATCATTTGTGTTATGATTTCGTGTATCCAGATATTAAAAATGTTTTGCG
TTTTTTAGATAATCACGATACTGATAGGTTTTTACGTTCGGAGCCTAAAGACTTAGATGGTTATAAACAAGGGGTTGCCT
TTTTATTAACAATCCCTGGCACTCCTCAATTATATTATGGACATGAATTGTTAATGTCTGGGACGAAAAGTCGCCCGGGT
GGGGATGGGAATATACGTTTAGATGTTCCAGGAGGGTGGCCTGGAGATTCTCAGAATTGGTTTACGAGGGAAGGACGTTC
CGACCTACAGAATAAGGCTTGGGATTATTTACAGACTTTATTGAAGTGGAGGAAGGGCAATAAGATCATTTCCGAAGGTA
ATATGAAACATTATATGCCACAATTAGGAGTGTATGTATATGAGCGTTATCTAGAGGGTAAAAGTGTCATGGTCATTATT
AATGGAGCTAATCATGAAGTTGATCTTCCACTTGCCCGTTATAAGGAATCTTTAAAAGAGGGAATAGAAGGAAAGGATAT
AATAACGAAACGAACGATCTTATTTAAGGACAGCTTGAAGCTGGCATCTAAAGAGGTATTGGTCGTTGAGATGAATTAA

Upstream 100 bases:

>100_bases
AGAATGGTAATCCTGATGTTAATATGAATTAACTTGGCTGTTTAAAAAGGGTGAGTTTGGCTCACCCTTTATTTGCTTTT
ACATCTAAATGATTATAGCT

Downstream 100 bases:

>100_bases
GAGATTATGAAAATAAACAGACGACAATTCGTAAGAAACTTAGGATTGGGCACAGCCTCGTTGAGTATGGGAAGTTTGAT
ATCGACTTCTTCTTGTGCCC

Product: glycoside hydrolase family alpha-glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 612; Mature: 612

Protein sequence:

>612_residues
MIRKIIIFTSFLLFVLGTVAAQTINKVEPLFWWAGMRNPELQLMVYGDHISEYHPVINEPGVYLKSCVTVDSPNYLILYL
DLSQAHSGTFDITFVNGEKKFVYAYELKERKESTDDIQGYDSSDVLYLIMPDRFANGDQSNDQISMSTEYVMDRSKPGAR
HGGDLKGIEDHLDYFVDLGVTAIWLNPVLENDGKGGSYHGYFSTDMFHVDRRLGSNEDYLRLINKAHQKGLRVVMDMIFN
HIGANHPWVLDVPSKDWFNVSPSKRGNHAKAIFYDNYASTYDSEVMKYAGFGIDLNQANPHVGKYLIQNSIWWIEYARID
AIRQDTYPYSDFDMMREWNIQIMNEYPQFNIVGEIWTNYTIGTAWWQKGCKLNFGKDTELKSVMDFTLMSIASDIFNDKS
SPEGRLDKIYDHLCYDFVYPDIKNVLRFLDNHDTDRFLRSEPKDLDGYKQGVAFLLTIPGTPQLYYGHELLMSGTKSRPG
GDGNIRLDVPGGWPGDSQNWFTREGRSDLQNKAWDYLQTLLKWRKGNKIISEGNMKHYMPQLGVYVYERYLEGKSVMVII
NGANHEVDLPLARYKESLKEGIEGKDIITKRTILFKDSLKLASKEVLVVEMN

Sequences:

>Translated_612_residues
MIRKIIIFTSFLLFVLGTVAAQTINKVEPLFWWAGMRNPELQLMVYGDHISEYHPVINEPGVYLKSCVTVDSPNYLILYL
DLSQAHSGTFDITFVNGEKKFVYAYELKERKESTDDIQGYDSSDVLYLIMPDRFANGDQSNDQISMSTEYVMDRSKPGAR
HGGDLKGIEDHLDYFVDLGVTAIWLNPVLENDGKGGSYHGYFSTDMFHVDRRLGSNEDYLRLINKAHQKGLRVVMDMIFN
HIGANHPWVLDVPSKDWFNVSPSKRGNHAKAIFYDNYASTYDSEVMKYAGFGIDLNQANPHVGKYLIQNSIWWIEYARID
AIRQDTYPYSDFDMMREWNIQIMNEYPQFNIVGEIWTNYTIGTAWWQKGCKLNFGKDTELKSVMDFTLMSIASDIFNDKS
SPEGRLDKIYDHLCYDFVYPDIKNVLRFLDNHDTDRFLRSEPKDLDGYKQGVAFLLTIPGTPQLYYGHELLMSGTKSRPG
GDGNIRLDVPGGWPGDSQNWFTREGRSDLQNKAWDYLQTLLKWRKGNKIISEGNMKHYMPQLGVYVYERYLEGKSVMVII
NGANHEVDLPLARYKESLKEGIEGKDIITKRTILFKDSLKLASKEVLVVEMN
>Mature_612_residues
MIRKIIIFTSFLLFVLGTVAAQTINKVEPLFWWAGMRNPELQLMVYGDHISEYHPVINEPGVYLKSCVTVDSPNYLILYL
DLSQAHSGTFDITFVNGEKKFVYAYELKERKESTDDIQGYDSSDVLYLIMPDRFANGDQSNDQISMSTEYVMDRSKPGAR
HGGDLKGIEDHLDYFVDLGVTAIWLNPVLENDGKGGSYHGYFSTDMFHVDRRLGSNEDYLRLINKAHQKGLRVVMDMIFN
HIGANHPWVLDVPSKDWFNVSPSKRGNHAKAIFYDNYASTYDSEVMKYAGFGIDLNQANPHVGKYLIQNSIWWIEYARID
AIRQDTYPYSDFDMMREWNIQIMNEYPQFNIVGEIWTNYTIGTAWWQKGCKLNFGKDTELKSVMDFTLMSIASDIFNDKS
SPEGRLDKIYDHLCYDFVYPDIKNVLRFLDNHDTDRFLRSEPKDLDGYKQGVAFLLTIPGTPQLYYGHELLMSGTKSRPG
GDGNIRLDVPGGWPGDSQNWFTREGRSDLQNKAWDYLQTLLKWRKGNKIISEGNMKHYMPQLGVYVYERYLEGKSVMVII
NGANHEVDLPLARYKESLKEGIEGKDIITKRTILFKDSLKLASKEVLVVEMN

Specific function: Hydrolyzes pullulan efficiently but only a small amount of starch. Endohydrolysis of 1,4-alpha-glucosidic linkages in pullulan to form panose. Cleaves also (1-6)-alpha-glucosidic linkages to form maltotriose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1789995, Length=149, Percent_Identity=36.9127516778523, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1786604, Length=170, Percent_Identity=32.3529411764706, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1790687, Length=175, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321731, Length=184, Percent_Identity=29.8913043478261, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6319776, Length=184, Percent_Identity=29.8913043478261, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6322245, Length=373, Percent_Identity=24.6648793565684, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6321726, Length=183, Percent_Identity=27.8688524590164, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6324416, Length=183, Percent_Identity=27.8688524590164, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322241, Length=183, Percent_Identity=27.8688524590164, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322021, Length=183, Percent_Identity=27.8688524590164, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24583745, Length=546, Percent_Identity=22.7106227106227, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.135 [H]

Molecular weight: Translated: 70522; Mature: 70522

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRKIIIFTSFLLFVLGTVAAQTINKVEPLFWWAGMRNPELQLMVYGDHISEYHPVINEP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCCHHCCCCCCCC
GVYLKSCVTVDSPNYLILYLDLSQAHSGTFDITFVNGEKKFVYAYELKERKESTDDIQGY
CCEEEEEEEECCCCEEEEEEEECCCCCCEEEEEEECCCCEEEEEEEHHHHCCCCCCCCCC
DSSDVLYLIMPDRFANGDQSNDQISMSTEYVMDRSKPGARHGGDLKGIEDHLDYFVDLGV
CCCCEEEEEECCCCCCCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCE
TAIWLNPVLENDGKGGSYHGYFSTDMFHVDRRLGSNEDYLRLINKAHQKGLRVVMDMIFN
EEEEECHHHCCCCCCCCCCCEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HIGANHPWVLDVPSKDWFNVSPSKRGNHAKAIFYDNYASTYDSEVMKYAGFGIDLNQANP
HHCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEECCCCC
HVGKYLIQNSIWWIEYARIDAIRQDTYPYSDFDMMREWNIQIMNEYPQFNIVGEIWTNYT
HHHHHHHHCCEEEEEEHHHHHHHHCCCCCCCHHHHHHCCEEEEECCCCEEEEEHHHCCCE
IGTAWWQKGCKLNFGKDTELKSVMDFTLMSIASDIFNDKSSPEGRLDKIYDHLCYDFVYP
EEHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC
DIKNVLRFLDNHDTDRFLRSEPKDLDGYKQGVAFLLTIPGTPQLYYGHELLMSGTKSRPG
CHHHHHHHHCCCCHHHHHHCCCCCCCHHHCCEEEEEECCCCCCEEECCHHHHCCCCCCCC
GDGNIRLDVPGGWPGDSQNWFTREGRSDLQNKAWDYLQTLLKWRKGNKIISEGNMKHYMP
CCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHH
QLGVYVYERYLEGKSVMVIINGANHEVDLPLARYKESLKEGIEGKDIITKRTILFKDSLK
HHHHHHHHHHHCCCEEEEEEECCCCEECCCHHHHHHHHHHCCCCCCEEEEEEEEEECCHH
LASKEVLVVEMN
HCCCCEEEEEEC
>Mature Secondary Structure
MIRKIIIFTSFLLFVLGTVAAQTINKVEPLFWWAGMRNPELQLMVYGDHISEYHPVINEP
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEECCCCHHCCCCCCCC
GVYLKSCVTVDSPNYLILYLDLSQAHSGTFDITFVNGEKKFVYAYELKERKESTDDIQGY
CCEEEEEEEECCCCEEEEEEEECCCCCCEEEEEEECCCCEEEEEEEHHHHCCCCCCCCCC
DSSDVLYLIMPDRFANGDQSNDQISMSTEYVMDRSKPGARHGGDLKGIEDHLDYFVDLGV
CCCCEEEEEECCCCCCCCCCCCEEEEEHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCE
TAIWLNPVLENDGKGGSYHGYFSTDMFHVDRRLGSNEDYLRLINKAHQKGLRVVMDMIFN
EEEEECHHHCCCCCCCCCCCEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HIGANHPWVLDVPSKDWFNVSPSKRGNHAKAIFYDNYASTYDSEVMKYAGFGIDLNQANP
HHCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEECCCCC
HVGKYLIQNSIWWIEYARIDAIRQDTYPYSDFDMMREWNIQIMNEYPQFNIVGEIWTNYT
HHHHHHHHCCEEEEEEHHHHHHHHCCCCCCCHHHHHHCCEEEEECCCCEEEEEHHHCCCE
IGTAWWQKGCKLNFGKDTELKSVMDFTLMSIASDIFNDKSSPEGRLDKIYDHLCYDFVYP
EEHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC
DIKNVLRFLDNHDTDRFLRSEPKDLDGYKQGVAFLLTIPGTPQLYYGHELLMSGTKSRPG
CHHHHHHHHCCCCHHHHHHCCCCCCCHHHCCEEEEEECCCCCCEEECCHHHHCCCCCCCC
GDGNIRLDVPGGWPGDSQNWFTREGRSDLQNKAWDYLQTLLKWRKGNKIISEGNMKHYMP
CCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHH
QLGVYVYERYLEGKSVMVIINGANHEVDLPLARYKESLKEGIEGKDIITKRTILFKDSLK
HHHHHHHHHHHCCCEEEEEEECCCCEECCCHHHHHHHHHHCCCCCCEEEEEEEEEECCHH
LASKEVLVVEMN
HCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2482332; 12547200 [H]