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The map label for this gene is tpiA

Identifier: 150009293

GI number: 150009293

Start: 3201936

End: 3202691

Strand: Reverse

Name: tpiA

Synonym: BDI_2695

Alternate gene names: 150009293

Gene position: 3202691-3201936 (Counterclockwise)

Preceding gene: 150009294

Following gene: 150009292

Centisome position: 66.56

GC content: 48.81

Gene sequence:

>756_bases
ATGAGAAAGAATATTGTTGCAGGAAACTGGAAAATGAACACTACTCTTGCTGAAGGTCTTGCTTTGGCTAAAGGTTTGGA
CGAGGCTTTGAAGGGTAAGACTCCGAATTGCGATGTGATCATCGGTACTCCGTTCACTCACTTGGCAAGCGTTGCTGCCG
CTATCGATACAAACAAGATCGGTGTTGCCGCTGAGAACTGCGCTGACAAGGAAAAAGGTGCTTATACAGGTGAGGTTTCT
GCCGCTATGGTAGCTTCTACTGGTGCGAAATATGTGATCTTAGGCCACTCTGAGAGACGTGCTTATTATCATGAGACTCC
GGAAATCTTGAAGACGAAGGTTGAGTTGGCTTTGGCTAACGGTTTGACTCCGATCTTCTGCATCGGTGAGGTTTTGGAAG
AGAGAGAAGCCGGTAAGCACTTCGAGGTAGTAGACGCTCAGATCGCTGGTTCTTTGTTCGACCTTTCCGCTGAGGATTTC
GGTAAGATCATCTTGGCTTACGAGCCGGTATGGGCTATCGGCACGGGTAAGACTGCTTCCGCTGAGCAAGCTGAGGAGAT
CCACGCTCACATCCGCGCTACTTTGGCCGCTAAATATGGCAATGAGGTAGCTGATAACTGCACGATCCTTTACGGTGGTA
GCTGTAACGCCGGTAACGCTAAGGAATTGTTCGCTAAACCGAACGTAGACGGTGGTTTGATCGGTGGCGCTTCTTTGGCC
GTTGACAAATTCATGCCGATTATCGAGGCTTTTTAA

Upstream 100 bases:

>100_bases
GCTGGTTTGGGGATACGATGCCTTGCGCAATCGCCGTCGCCGGAAAGGAAAAGAATCTGAATAGAGATTTATTTGTATTA
CATATTTAAATTATTAAGTT

Downstream 100 bases:

>100_bases
TTAATTCATAATAAGAAGGTATCTTTCCCCTCAATGAATGGGGAAAGATACTCTTTTTAATATTCTATCTATGAAAAGAA
TTCCTTGTATCTTATTGCTA

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MRKNIVAGNWKMNTTLAEGLALAKGLDEALKGKTPNCDVIIGTPFTHLASVAAAIDTNKIGVAAENCADKEKGAYTGEVS
AAMVASTGAKYVILGHSERRAYYHETPEILKTKVELALANGLTPIFCIGEVLEEREAGKHFEVVDAQIAGSLFDLSAEDF
GKIILAYEPVWAIGTGKTASAEQAEEIHAHIRATLAAKYGNEVADNCTILYGGSCNAGNAKELFAKPNVDGGLIGGASLA
VDKFMPIIEAF

Sequences:

>Translated_251_residues
MRKNIVAGNWKMNTTLAEGLALAKGLDEALKGKTPNCDVIIGTPFTHLASVAAAIDTNKIGVAAENCADKEKGAYTGEVS
AAMVASTGAKYVILGHSERRAYYHETPEILKTKVELALANGLTPIFCIGEVLEEREAGKHFEVVDAQIAGSLFDLSAEDF
GKIILAYEPVWAIGTGKTASAEQAEEIHAHIRATLAAKYGNEVADNCTILYGGSCNAGNAKELFAKPNVDGGLIGGASLA
VDKFMPIIEAF
>Mature_251_residues
MRKNIVAGNWKMNTTLAEGLALAKGLDEALKGKTPNCDVIIGTPFTHLASVAAAIDTNKIGVAAENCADKEKGAYTGEVS
AAMVASTGAKYVILGHSERRAYYHETPEILKTKVELALANGLTPIFCIGEVLEEREAGKHFEVVDAQIAGSLFDLSAEDF
GKIILAYEPVWAIGTGKTASAEQAEEIHAHIRATLAAKYGNEVADNCTILYGGSCNAGNAKELFAKPNVDGGLIGGASLA
VDKFMPIIEAF

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI226529917, Length=250, Percent_Identity=44.4, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI4507645, Length=250, Percent_Identity=44.4, Blast_Score=189, Evalue=2e-48,
Organism=Escherichia coli, GI1790353, Length=250, Percent_Identity=43.6, Blast_Score=195, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17536593, Length=249, Percent_Identity=45.7831325301205, Blast_Score=193, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6320255, Length=250, Percent_Identity=42.4, Blast_Score=187, Evalue=1e-48,
Organism=Drosophila melanogaster, GI28572008, Length=249, Percent_Identity=47.3895582329317, Blast_Score=205, Evalue=3e-53,
Organism=Drosophila melanogaster, GI28572006, Length=249, Percent_Identity=47.3895582329317, Blast_Score=205, Evalue=3e-53,
Organism=Drosophila melanogaster, GI28572004, Length=249, Percent_Identity=47.3895582329317, Blast_Score=204, Evalue=4e-53,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_PARD8 (A6LFF0)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001304036.1
- ProteinModelPortal:   A6LFF0
- SMR:   A6LFF0
- STRING:   A6LFF0
- GeneID:   5307844
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_2695
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   PDIS435591:BDI_2695-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26428; Mature: 26428

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00171 TIM_1; PS51440 TIM_2; PS00107 PROTEIN_KINASE_ATP

Important sites: ACT_SITE 96-96 ACT_SITE 168-168 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKNIVAGNWKMNTTLAEGLALAKGLDEALKGKTPNCDVIIGTPFTHLASVAAAIDTNKI
CCCCEEECCCEECHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHCCCCC
GVAAENCADKEKGAYTGEVSAAMVASTGAKYVILGHSERRAYYHETPEILKTKVELALAN
CEEHHHCCCCCCCCCCCCHHHHHEECCCCCEEEEECCCCCCHHCCCHHHHHHHHHHHHHC
GLTPIFCIGEVLEEREAGKHFEVVDAQIAGSLFDLSAEDFGKIILAYEPVWAIGTGKTAS
CCCHHHHHHHHHHHHHCCCCEEEEHHHHHCHHHCCCHHHHCCEEEEECCEEEECCCCCCC
AEQAEEIHAHIRATLAAKYGNEVADNCTILYGGSCNAGNAKELFAKPNVDGGLIGGASLA
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHEECCCCCCCEECCHHHH
VDKFMPIIEAF
HHHHHHHHHCC
>Mature Secondary Structure
MRKNIVAGNWKMNTTLAEGLALAKGLDEALKGKTPNCDVIIGTPFTHLASVAAAIDTNKI
CCCCEEECCCEECHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHCCCCC
GVAAENCADKEKGAYTGEVSAAMVASTGAKYVILGHSERRAYYHETPEILKTKVELALAN
CEEHHHCCCCCCCCCCCCHHHHHEECCCCCEEEEECCCCCCHHCCCHHHHHHHHHHHHHC
GLTPIFCIGEVLEEREAGKHFEVVDAQIAGSLFDLSAEDFGKIILAYEPVWAIGTGKTAS
CCCHHHHHHHHHHHHHCCCCEEEEHHHHHCHHHCCCHHHHCCEEEEECCEEEECCCCCCC
AEQAEEIHAHIRATLAAKYGNEVADNCTILYGGSCNAGNAKELFAKPNVDGGLIGGASLA
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHEECCCCCCCEECCHHHH
VDKFMPIIEAF
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA