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The map label for this gene is ddl

Identifier: 150009273

GI number: 150009273

Start: 3176891

End: 3177883

Strand: Reverse

Name: ddl

Synonym: BDI_2675

Alternate gene names: 150009273

Gene position: 3177883-3176891 (Counterclockwise)

Preceding gene: 150009274

Following gene: 150009272

Centisome position: 66.05

GC content: 45.22

Gene sequence:

>993_bases
ATGAAAAAGAACATCGCTATCGTAGCAGGGGGAGATTCCTCCGAGATCGTTATTTCATTAAAAAGTGCGGACGGAATCTA
CTCTTTTATTGATAAAGACAAATATAATTTATATATTGCCATCGTTAAGAGGGATGAGTGGGCGGTTATACTTCCTAGCG
GGGAACATACACCAATTGATAAGAATGATTTTAGCTTCAAGGAGAATGGCGTGACGAGGCATTTTGACTTCGCTTATATA
ACGATACATGGTACTCCGGGCGAGGACGGACGTTTGCAAGGATATTTCGATATGATCGGTATGCCTTACTCGTCTTGCGG
GATGCTGGTGAGCGCTTTGACATTCAATAAATACGTATGTAATCATTACTTGAAAGACTTTGGTGTAAAAATTGCCGCGT
CCATTCATTTATTCAAGGGAGAGACTATTACGGACGAAGAAGTCGTGACTCGTTTGGGTTTGCCTATTTTCGTGAAACCA
AACGATGGGGGTAGTAGTTTTGGCGTGACGAAGGTGAAAGAGGTCTCCGCTATCCAGCCCGCTATCGCTAAGGCGTTTGG
GGAAGGACGTGAGGTGATTCTCGAGCGCTTTATCGATGGAACGGAAGTGACATGCGGTTGCTATAAGGTGGAGGGTAAAG
AGGTTGTCTTCCCTTTGACCGAGGTGGTTACGAATAACGAATTCTTCGATTTCGACGCGAAGTATAACGGACAGGTGGAC
GAGATCACTCCGGCCCGTATCTCCACCGAGTTGACGGAGTTGATACAGTGCGAGACATCAAGGATCTACGATATATTGGG
CGCCAAAGGACTGATTCGTGTGGATTATATTATTCCGGCAGACGGGGAGCCTGTCTTACTGGAAATAAACACGACACCGG
GAATGACGGCTACCAGCTTTATCCCACAGCAAGTACGTGCGGCGGGATTGGATATAAAGGATGTCATGACAGACATTATT
GAAGACGAACTCAAAAACAGAAAAACAAAATGA

Upstream 100 bases:

>100_bases
CTACGGGAGAGGAGATGTTTTTCGATTCTGAGATTCCTTCCGATATGCAGCAGCTGATCGACAGATGGCGTGTATACGCG
AACACTAAAGAATTGTAAAA

Downstream 100 bases:

>100_bases
ATAGATATGGATACAGAAACAGTTTCCCCTAATTTCGACGACATCCGTCCGCTGAACAACAGTGAGGTGAAGGACGCGAT
TGAAAAATTGGTCGCCAATG

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKKNIAIVAGGDSSEIVISLKSADGIYSFIDKDKYNLYIAIVKRDEWAVILPSGEHTPIDKNDFSFKENGVTRHFDFAYI
TIHGTPGEDGRLQGYFDMIGMPYSSCGMLVSALTFNKYVCNHYLKDFGVKIAASIHLFKGETITDEEVVTRLGLPIFVKP
NDGGSSFGVTKVKEVSAIQPAIAKAFGEGREVILERFIDGTEVTCGCYKVEGKEVVFPLTEVVTNNEFFDFDAKYNGQVD
EITPARISTELTELIQCETSRIYDILGAKGLIRVDYIIPADGEPVLLEINTTPGMTATSFIPQQVRAAGLDIKDVMTDII
EDELKNRKTK

Sequences:

>Translated_330_residues
MKKNIAIVAGGDSSEIVISLKSADGIYSFIDKDKYNLYIAIVKRDEWAVILPSGEHTPIDKNDFSFKENGVTRHFDFAYI
TIHGTPGEDGRLQGYFDMIGMPYSSCGMLVSALTFNKYVCNHYLKDFGVKIAASIHLFKGETITDEEVVTRLGLPIFVKP
NDGGSSFGVTKVKEVSAIQPAIAKAFGEGREVILERFIDGTEVTCGCYKVEGKEVVFPLTEVVTNNEFFDFDAKYNGQVD
EITPARISTELTELIQCETSRIYDILGAKGLIRVDYIIPADGEPVLLEINTTPGMTATSFIPQQVRAAGLDIKDVMTDII
EDELKNRKTK
>Mature_330_residues
MKKNIAIVAGGDSSEIVISLKSADGIYSFIDKDKYNLYIAIVKRDEWAVILPSGEHTPIDKNDFSFKENGVTRHFDFAYI
TIHGTPGEDGRLQGYFDMIGMPYSSCGMLVSALTFNKYVCNHYLKDFGVKIAASIHLFKGETITDEEVVTRLGLPIFVKP
NDGGSSFGVTKVKEVSAIQPAIAKAFGEGREVILERFIDGTEVTCGCYKVEGKEVVFPLTEVVTNNEFFDFDAKYNGQVD
EITPARISTELTELIQCETSRIYDILGAKGLIRVDYIIPADGEPVLLEINTTPGMTATSFIPQQVRAAGLDIKDVMTDII
EDELKNRKTK

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=327, Percent_Identity=29.3577981651376, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1786579, Length=362, Percent_Identity=32.5966850828729, Blast_Score=160, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_PARD8 (A6LFD0)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001304016.1
- ProteinModelPortal:   A6LFD0
- SMR:   A6LFD0
- STRING:   A6LFD0
- GeneID:   5307824
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_2675
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   GNEMIAH
- ProtClustDB:   PRK01966
- BioCyc:   PDIS435591:BDI_2675-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 36498; Mature: 36498

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNIAIVAGGDSSEIVISLKSADGIYSFIDKDKYNLYIAIVKRDEWAVILPSGEHTPID
CCCCEEEEECCCCCEEEEEEECCCCEEHHHCCCCCEEEEEEEECCCEEEEEECCCCCCCC
KNDFSFKENGVTRHFDFAYITIHGTPGEDGRLQGYFDMIGMPYSSCGMLVSALTFNKYVC
CCCCCCCCCCCEEEEEEEEEEEECCCCCCCCEEEEEHCCCCCHHHHHHHHHHHHHHHHHH
NHYLKDFGVKIAASIHLFKGETITDEEVVTRLGLPIFVKPNDGGSSFGVTKVKEVSAIQP
HHHHHHCCEEEEEEEEEEECCCCCHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHH
AIAKAFGEGREVILERFIDGTEVTCGCYKVEGKEVVFPLTEVVTNNEFFDFDAKYNGQVD
HHHHHHCCHHHHHHHHHCCCCCEEEEEEEECCCEEEEEHHHHHCCCCEEEECCCCCCCCC
EITPARISTELTELIQCETSRIYDILGAKGLIRVDYIIPADGEPVLLEINTTPGMTATSF
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEEECCCCCCHHHH
IPQQVRAAGLDIKDVMTDIIEDELKNRKTK
CHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKNIAIVAGGDSSEIVISLKSADGIYSFIDKDKYNLYIAIVKRDEWAVILPSGEHTPID
CCCCEEEEECCCCCEEEEEEECCCCEEHHHCCCCCEEEEEEEECCCEEEEEECCCCCCCC
KNDFSFKENGVTRHFDFAYITIHGTPGEDGRLQGYFDMIGMPYSSCGMLVSALTFNKYVC
CCCCCCCCCCCEEEEEEEEEEEECCCCCCCCEEEEEHCCCCCHHHHHHHHHHHHHHHHHH
NHYLKDFGVKIAASIHLFKGETITDEEVVTRLGLPIFVKPNDGGSSFGVTKVKEVSAIQP
HHHHHHCCEEEEEEEEEEECCCCCHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHH
AIAKAFGEGREVILERFIDGTEVTCGCYKVEGKEVVFPLTEVVTNNEFFDFDAKYNGQVD
HHHHHHCCHHHHHHHHHCCCCCEEEEEEEECCCEEEEEHHHHHCCCCEEEECCCCCCCCC
EITPARISTELTELIQCETSRIYDILGAKGLIRVDYIIPADGEPVLLEINTTPGMTATSF
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEEECCCCCCHHHH
IPQQVRAAGLDIKDVMTDIIEDELKNRKTK
CHHHHHHCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA