Click here to switch to the map view.

The map label for this gene is ksgA

Identifier: 150009182

GI number: 150009182

Start: 3062230

End: 3063030

Strand: Direct

Name: ksgA

Synonym: BDI_2584

Alternate gene names: 150009182

Gene position: 3062230-3063030 (Clockwise)

Preceding gene: 150009179

Following gene: 150009183

Centisome position: 63.65

GC content: 47.32

Gene sequence:

>801_bases
ATGAGATTAGTTAAGCCAAAAAAAGCATTAGGGCAACATTTCCTAAAGGATCTGCAAATAGCACAGCGCATTGCGGACAC
ACTCGATACTTTCAAATCCTTGCCGGTCTTGGAAATCGGACCGGGAATGGGAGTCCTTACCCAGTTCTTGCTGGAGGCGG
GACATGATTTGACCGTTGTCGAGTTGGACATGGAATCCGTAGATTACCTGAACCAGAATTTCCCCGATCTGAAAGGACGT
ATCATCGCCGAGGATTTCCTGAAATTGGATCTCTCGAAGCTATTTCCGGGGCAGTTTTGTGTGATCGGTAATTATCCCTA
TAATATCTCCAGCCAGATATTCTTCAAAGTCCTCGATTATAAGGACCAGATTCCCTGCTGCTCGGGGATGTTACAAAAAG
AGGTAGCGGAACGCCTAGCGGCAGGTCCCGGTAGCAAGACGTACGGAATCTTGAGCGTATTGCTTCAAGCTTGGTACGAC
GTGGAATACCTCTTTACTGTCAGTGAGAAAGTATTCGATCCGCCTCCAAAGGTGAAAAGCGCCGTATTGAAAATGACTCG
TAACGACCGCAAGGAGCTGGGATGCGACGAGAAGTTATTCAAGACCGTAGTAAAGACCTCATTCAACCAACGAAGAAAAA
CGCTTCGTAATTCCATGAAACCCCTATTGGGTAAAGATTGCCCGGATTACGCCTTGCCGATATTCGACAAGCGCCCGGAA
CAGTTATCCATGGAGCAATTCGTGGAACTGACCTTGATCAGCCAAAAGCATCTGGAGAAGCAGGCTGGTGATGTCCTATA
A

Upstream 100 bases:

>100_bases
TCCATTAGGTATAGGGAACTTTAAATTATTTATTGTACTTTTGTGCATCCGCAAAGATAAGCATAATATTTAAAAACAAA
CACATTACACCGAAAAGTGG

Downstream 100 bases:

>100_bases
TAATATAAGAGTCCAATGATCGAACTGACGAAAGATTACATCGAGAACCTGAAAAGCATCATCGAGGCCAAAGATGACGC
GAAGGCACAGGAGGTACTTC

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MRLVKPKKALGQHFLKDLQIAQRIADTLDTFKSLPVLEIGPGMGVLTQFLLEAGHDLTVVELDMESVDYLNQNFPDLKGR
IIAEDFLKLDLSKLFPGQFCVIGNYPYNISSQIFFKVLDYKDQIPCCSGMLQKEVAERLAAGPGSKTYGILSVLLQAWYD
VEYLFTVSEKVFDPPPKVKSAVLKMTRNDRKELGCDEKLFKTVVKTSFNQRRKTLRNSMKPLLGKDCPDYALPIFDKRPE
QLSMEQFVELTLISQKHLEKQAGDVL

Sequences:

>Translated_266_residues
MRLVKPKKALGQHFLKDLQIAQRIADTLDTFKSLPVLEIGPGMGVLTQFLLEAGHDLTVVELDMESVDYLNQNFPDLKGR
IIAEDFLKLDLSKLFPGQFCVIGNYPYNISSQIFFKVLDYKDQIPCCSGMLQKEVAERLAAGPGSKTYGILSVLLQAWYD
VEYLFTVSEKVFDPPPKVKSAVLKMTRNDRKELGCDEKLFKTVVKTSFNQRRKTLRNSMKPLLGKDCPDYALPIFDKRPE
QLSMEQFVELTLISQKHLEKQAGDVL
>Mature_266_residues
MRLVKPKKALGQHFLKDLQIAQRIADTLDTFKSLPVLEIGPGMGVLTQFLLEAGHDLTVVELDMESVDYLNQNFPDLKGR
IIAEDFLKLDLSKLFPGQFCVIGNYPYNISSQIFFKVLDYKDQIPCCSGMLQKEVAERLAAGPGSKTYGILSVLLQAWYD
VEYLFTVSEKVFDPPPKVKSAVLKMTRNDRKELGCDEKLFKTVVKTSFNQRRKTLRNSMKPLLGKDCPDYALPIFDKRPE
QLSMEQFVELTLISQKHLEKQAGDVL

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=268, Percent_Identity=32.089552238806, Blast_Score=101, Evalue=7e-22,
Organism=Homo sapiens, GI156415992, Length=283, Percent_Identity=30.7420494699647, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1786236, Length=256, Percent_Identity=35.9375, Blast_Score=133, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI25146882, Length=266, Percent_Identity=29.6992481203008, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI25141369, Length=257, Percent_Identity=26.0700389105058, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324989, Length=216, Percent_Identity=32.8703703703704, Blast_Score=102, Evalue=6e-23,
Organism=Drosophila melanogaster, GI21358017, Length=218, Percent_Identity=32.5688073394495, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI21357273, Length=278, Percent_Identity=28.4172661870504, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_PARD8 (A6LF39)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001303925.1
- ProteinModelPortal:   A6LF39
- SMR:   A6LF39
- STRING:   A6LF39
- GeneID:   5307733
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_2584
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- ProtClustDB:   PRK00274
- BioCyc:   PDIS435591:BDI_2584-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30227; Mature: 30227

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 13-13 BINDING 15-15 BINDING 40-40 BINDING 61-61 BINDING 85-85 BINDING 104-104

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure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HHCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA