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The map label for this gene is mdeA [H]

Identifier: 150009150

GI number: 150009150

Start: 3024030

End: 3025319

Strand: Direct

Name: mdeA [H]

Synonym: BDI_2552

Alternate gene names: 150009150

Gene position: 3024030-3025319 (Clockwise)

Preceding gene: 150009145

Following gene: 150009159

Centisome position: 62.85

GC content: 47.36

Gene sequence:

>1290_bases
ATGGCAACAAAGAAGTTACATTTCGAGACATTACAACTTCATGTAGGACAAGAACAACCGGATCCCGCAACCGACGCACG
TGCAGTACCCATTTATCAAACAACTTCCTATGTATTCCATAACGCACAACATGCGGCAGACCGTTTCGGCCTACGTGACG
CAGGGAATATTTACGGACGTTTGACAAACTCCACGCAAGGCGTTTTCGAGGCACGTGTAGCTGCCTTAGAAGGAGGTGTG
GCAGGTTTGGCCGTGGCTTCAGGAGCAGCGGCTGTCACCTATGCGTTACAGAACATAGTGAGGGCAGGAGATCATATTAT
CGCGGCCGATAATTTGTATGGCGGTTCCTTCAACCTGATCACCCATACACTGGCCTCCCAAGGCATTACTAATACGATTA
TCAATGTAAATGACCTAAACGCATTAGAAGCAGCCATCCGGGAAAACACGAAAGCCATTTATGTAGAGACATTCGGGAAT
CCTAACTCTGACGTAACGAATCTGGATGCGGTAGCGGAAGTCGCTCACCGATACCATGTTCCATTAATCGTAGACAATAC
GTTCGGAACCCCCTACTTGATTCGTCCGATCGAACATGGAGCTGACATTGTCATCCATTCCGCCACAAAGTTCATAGGAG
GACACGGCAGTAGCCTAGGAGGTGTAATTGTAGACGGAGGTACTTTCGACTGGAAGGCGAATGCAGATAAGTTCCCTACA
TTAGCTAAACCAGACCCAAGTTATCATGGAGCCATATTTGCGGACGTAGCAGGTTCCGCCGCCTTTGTCACCCGTATCCG
TGCGGTCATTCTTCGCGATACCGGGGCAACGATCTCTCCTTTCAATGCTTTTATATTGTTGCAAGGATTAGAGACCTTAT
CCCTACGCGTGGAGCGCCATGTAGCAAACGCATTGAAAGTCGTGGATTATTTATCAAAACATCCGAAAGTAGAAAAAGTG
AATCATCCATCACTTCCTACCCATCCGGACCATGAACTTTACAATAAATATTTCCCGAATGGTGCCGGCAGTATCTTCAC
CTTCGAGATCAAAGGCGGGCAGGAAGCCGCTTGGAAATTCATCGACTCACTGGAGATATTTTCCCTACTAGCGAATGTCG
CCGATGTAAAGAGCCTTGTCATTCATCCATACACGACCACTCATTCCCAGATGAGCCCGGAAGAACTTGCCCAACAGCAC
ATAACACCATCCACCGTACGCTTGAGCATCGGAACCGAATATATAGATGATATTATAGATGATCTGGATCAAGCTTTCGC
TAAAATCTGA

Upstream 100 bases:

>100_bases
AAAAATAGTATTATTTTCTATATTCTCAACGAATCTTGTCCAAAAAGCACACACATCATACATTTGCCAACAGAATAAGA
ACTAAATAAACAAGATGACT

Downstream 100 bases:

>100_bases
TAGCGCACTTCCAAAAGGAAAAGGACAGAAGCAAAGAAGGCGATCCCTGTTCGACACAAGGATCGTCTTTCTAATCAACA
TCACATCGAGCCAAGGTTAC

Product: O-acetylhomoserine (thiol)-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MATKKLHFETLQLHVGQEQPDPATDARAVPIYQTTSYVFHNAQHAADRFGLRDAGNIYGRLTNSTQGVFEARVAALEGGV
AGLAVASGAAAVTYALQNIVRAGDHIIAADNLYGGSFNLITHTLASQGITNTIINVNDLNALEAAIRENTKAIYVETFGN
PNSDVTNLDAVAEVAHRYHVPLIVDNTFGTPYLIRPIEHGADIVIHSATKFIGGHGSSLGGVIVDGGTFDWKANADKFPT
LAKPDPSYHGAIFADVAGSAAFVTRIRAVILRDTGATISPFNAFILLQGLETLSLRVERHVANALKVVDYLSKHPKVEKV
NHPSLPTHPDHELYNKYFPNGAGSIFTFEIKGGQEAAWKFIDSLEIFSLLANVADVKSLVIHPYTTTHSQMSPEELAQQH
ITPSTVRLSIGTEYIDDIIDDLDQAFAKI

Sequences:

>Translated_429_residues
MATKKLHFETLQLHVGQEQPDPATDARAVPIYQTTSYVFHNAQHAADRFGLRDAGNIYGRLTNSTQGVFEARVAALEGGV
AGLAVASGAAAVTYALQNIVRAGDHIIAADNLYGGSFNLITHTLASQGITNTIINVNDLNALEAAIRENTKAIYVETFGN
PNSDVTNLDAVAEVAHRYHVPLIVDNTFGTPYLIRPIEHGADIVIHSATKFIGGHGSSLGGVIVDGGTFDWKANADKFPT
LAKPDPSYHGAIFADVAGSAAFVTRIRAVILRDTGATISPFNAFILLQGLETLSLRVERHVANALKVVDYLSKHPKVEKV
NHPSLPTHPDHELYNKYFPNGAGSIFTFEIKGGQEAAWKFIDSLEIFSLLANVADVKSLVIHPYTTTHSQMSPEELAQQH
ITPSTVRLSIGTEYIDDIIDDLDQAFAKI
>Mature_428_residues
ATKKLHFETLQLHVGQEQPDPATDARAVPIYQTTSYVFHNAQHAADRFGLRDAGNIYGRLTNSTQGVFEARVAALEGGVA
GLAVASGAAAVTYALQNIVRAGDHIIAADNLYGGSFNLITHTLASQGITNTIINVNDLNALEAAIRENTKAIYVETFGNP
NSDVTNLDAVAEVAHRYHVPLIVDNTFGTPYLIRPIEHGADIVIHSATKFIGGHGSSLGGVIVDGGTFDWKANADKFPTL
AKPDPSYHGAIFADVAGSAAFVTRIRAVILRDTGATISPFNAFILLQGLETLSLRVERHVANALKVVDYLSKHPKVEKVN
HPSLPTHPDHELYNKYFPNGAGSIFTFEIKGGQEAAWKFIDSLEIFSLLANVADVKSLVIHPYTTTHSQMSPEELAQQHI
TPSTVRLSIGTEYIDDIIDDLDQAFAKI

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=406, Percent_Identity=32.0197044334975, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI299473759, Length=407, Percent_Identity=28.7469287469287, Blast_Score=159, Evalue=7e-39,
Organism=Homo sapiens, GI25453487, Length=405, Percent_Identity=28.3950617283951, Blast_Score=149, Evalue=4e-36,
Organism=Escherichia coli, GI1790375, Length=400, Percent_Identity=30.75, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1789383, Length=437, Percent_Identity=26.5446224256293, Blast_Score=149, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71980708, Length=431, Percent_Identity=32.2505800464037, Blast_Score=221, Evalue=8e-58,
Organism=Caenorhabditis elegans, GI17538055, Length=411, Percent_Identity=31.8734793187348, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17560056, Length=410, Percent_Identity=31.219512195122, Blast_Score=194, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6323333, Length=435, Percent_Identity=45.0574712643678, Blast_Score=351, Evalue=1e-97,
Organism=Saccharomyces cerevisiae, GI6319307, Length=377, Percent_Identity=33.1564986737401, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6321170, Length=362, Percent_Identity=25.414364640884, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6321254, Length=423, Percent_Identity=25.0591016548463, Blast_Score=112, Evalue=8e-26,
Organism=Drosophila melanogaster, GI19922554, Length=421, Percent_Identity=34.916864608076, Blast_Score=218, Evalue=4e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46353; Mature: 46221

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKKLHFETLQLHVGQEQPDPATDARAVPIYQTTSYVFHNAQHAADRFGLRDAGNIYGR
CCCCEEEEEEEEEEECCCCCCCCCCCEEEEEEECHHHHHHCHHHHHHHCCCCCCCCEEEE
LTNSTQGVFEARVAALEGGVAGLAVASGAAAVTYALQNIVRAGDHIIAADNLYGGSFNLI
ECCCCCHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHH
THTLASQGITNTIINVNDLNALEAAIRENTKAIYVETFGNPNSDVTNLDAVAEVAHRYHV
HHHHHHCCCCCEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCC
PLIVDNTFGTPYLIRPIEHGADIVIHSATKFIGGHGSSLGGVIVDGGTFDWKANADKFPT
CEEEECCCCCCEEEEECCCCCCEEEEEHHHHHCCCCCCCCCEEEECCEEEECCCCCCCCC
LAKPDPSYHGAIFADVAGSAAFVTRIRAVILRDTGATISPFNAFILLQGLETLSLRVERH
CCCCCCCCCCEEEEECCCCHHHHHHHHHHEEECCCCCCCCHHHHHEECCHHHHHHHHHHH
VANALKVVDYLSKHPKVEKVNHPSLPTHPDHELYNKYFPNGAGSIFTFEIKGGQEAAWKF
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
IDSLEIFSLLANVADVKSLVIHPYTTTHSQMSPEELAQQHITPSTVRLSIGTEYIDDIID
HHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHCCCCCEEEEEECHHHHHHHHH
DLDQAFAKI
HHHHHHHCC
>Mature Secondary Structure 
ATKKLHFETLQLHVGQEQPDPATDARAVPIYQTTSYVFHNAQHAADRFGLRDAGNIYGR
CCCEEEEEEEEEEECCCCCCCCCCCEEEEEEECHHHHHHCHHHHHHHCCCCCCCCEEEE
LTNSTQGVFEARVAALEGGVAGLAVASGAAAVTYALQNIVRAGDHIIAADNLYGGSFNLI
ECCCCCHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHH
THTLASQGITNTIINVNDLNALEAAIRENTKAIYVETFGNPNSDVTNLDAVAEVAHRYHV
HHHHHHCCCCCEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCC
PLIVDNTFGTPYLIRPIEHGADIVIHSATKFIGGHGSSLGGVIVDGGTFDWKANADKFPT
CEEEECCCCCCEEEEECCCCCCEEEEEHHHHHCCCCCCCCCEEEECCEEEECCCCCCCCC
LAKPDPSYHGAIFADVAGSAAFVTRIRAVILRDTGATISPFNAFILLQGLETLSLRVERH
CCCCCCCCCCEEEEECCCCHHHHHHHHHHEEECCCCCCCCHHHHHEECCHHHHHHHHHHH
VANALKVVDYLSKHPKVEKVNHPSLPTHPDHELYNKYFPNGAGSIFTFEIKGGQEAAWKF
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCHHHHHHH
IDSLEIFSLLANVADVKSLVIHPYTTTHSQMSPEELAQQHITPSTVRLSIGTEYIDDIID
HHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHCCCCCEEEEEECHHHHHHHHH
DLDQAFAKI
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]