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The map label for this gene is spoT [H]

Identifier: 150009148

GI number: 150009148

Start: 3021008

End: 3023329

Strand: Reverse

Name: spoT [H]

Synonym: BDI_2550

Alternate gene names: 150009148

Gene position: 3023329-3021008 (Counterclockwise)

Preceding gene: 150009149

Following gene: 150009147

Centisome position: 62.84

GC content: 46.43

Gene sequence:

>2322_bases
GTGGCTATACAGTATAAAAATGGGAGGTGTAAAACCATGGACGATACAAAGGACACAAAAGATATAAAAGAAACGGGCGA
GGCTGCGTCCGTTCTCTCTGCGGACGAACAGATGATACAAGATGGGTTCAATGCGTTGTTGAACGATTATTTGAAGTCAA
ACCACCGCCGTAAGGTCGAACGAATCACGAAAGCTTTTAATTTCGCCAATCAAGCGCACGCCGGAGTGAAACGGCGTTCC
GGCGAACCTTATATAATGCATCCTATCGCCGTGGCTCGTATCGTATGCCGGGAGATGGGTTTGGGCTCTACCTCTATTTG
CTCTGCGTTATTGCATGATGTCGTGGAAGATACCGAGTATACGGTGGAGGATATCCGGGATATGTTCGGAGACAAGATCG
CCCAGATCGTGGATGGCTTGACGAAGATATCCGGTGGCATTTTTGGGGAACAGGCCTCGGCTCAAGCCGAGAACTTCCGT
AAGTTGCTGCTGACTATGAGTGACGATATACGGGTGATTCTAATCAAGATCGCAGACCGTCTTCATAATATGAGGACTTT
GGGTTCCATGCTTCCCGCCAAGCAATTTAAGATCGCGGGTGAGACTCTTTATCTGTATGCTCCTCTGGCGCATCGTCTGG
GTCTCTTCTCGATCAAGACGGAACTGGAGGATTTGAGTTTTAAATATGAGCATCCGCAAGAATATGACTTCATCAGCGCT
AAGTTGAAAGCGACAGAAGAGAGCCGTAATAAATTATTCGAGCGTTTCGCCGCCCCTGTGGACGAGAAGCTGAAATCGAT
GGGACTTCAGTATGAGATGCGTGCCCGTGTGAAATCGGTCTATTCCATTTGGAATAAGATGGAAAGCAAGGGTGTCGCTT
TCGAGGATATTTATGATATTTATGCGGTCCGCATTATTTTTGACCCGCTTCCCGGTGTGGACGAGAAAAACCAATGTTGG
GATATCTACTCGGCTATTACGGATATTTACCGGATTCGTCCCGACCGTATCCGTGATTGGGTGAGCCGTCCGAAAGCGAA
CGGTTATCAAGCGCTTCATCTGACCGTTATGGGACCGGATGGACAATGGGTAGAGATCCAGATCCGTAGCCGCCGTATGG
ATGATATCGCAGAGAAAGGCTTCGCTGCCCATTGGAAATACAAGGAATCCAACGTGGAGGAGGATACGGAATTGGATAAA
TGGATACAAACCATTACCGAGATCTTGGAGAGTCCGGACCCGAATGCGCTGGATTTCTTGGATACGATCAAACTGAACTT
ATTTACTTCCGAGATATTTGTTTTCACTCCGAAAGGAGATATAAAGACATTGCCCCAAGGGGCTACCGCCTTGGATTTCG
CTTATGCGCTTCACTCCGATATCGGTAATAAATGTATTGGCGCTAAGGTAAACCATCGTTTGGTGCCATTGAGTCATCCG
CTGTCCAGTGGAGATCAGGTGGAGGTCTTGACTTCCCGTTCTCAGGAGCCGCAACCGGAGTGGTTGAACTTCGTGACTAC
CGCTAAGGCTCGTGCGAAGATTGATGCCGTATTAAAGCGTGTCCGTAAAGAGGTCGCTAAGTATGGAGAGATAAAAGTTC
TTGATGCCTTCAAGCGTTCGGAGCTTGAGGCGAGTACATCCAATCTGGATAAATTAGGCATGTACTTTGGATTCTCGAAA
CGCGAGGAATTCTTTTATGCGGTAGAGAAGGGGGATGTGGTTTTGCCCGAGAATTTAAAGAAGCTGTTGAAGGAGAAGAC
GGATAATGTCTTGTTTAAGTATGTGAAACAGGCGTTGGGTGTCGCTTCCAAGAAGGTGAAACAACCCGAAGAGGAGGAAG
CCAAGAAAGAGAAGCCTAAATATGATAAGAAAAAGCCTTATTTATTGAAAGAGGAGGCTTTTGAGCGTAATTATGTAATT
GCCGAATGTTGTAAGCCGATTCCGGGAGATGACTCTTTAGGTTTTATCAATGATGATGGAAATGTAGTAGTTCATAAACG
TTCCTGCCCGATCGCCATGCGCTTGAAAAGTAGCTTTGGCGAACGTATCTTGAATACGGTTTGGAGCAGCCATATGAACG
CTTCTTTTGAGGCCACGCTAGAGGTGAAAGGTATAGACTCGATAGGCGTATTGAATACGATCACGAAAACGATTTCCGAG
GATTTCAACGTGAACATCATGCGCTTGCTGATCGAGGCGAAAGACGGCGTGTTCGAAGGTAAGATAAAGATGAAAGTTCA
TGACGTAGAAGATATCCAGAAAATGTGCGTGACTTTGTCGAAGATACAGAATATTAAATCTGTCGGACGTGTAGCGGATT
AA

Upstream 100 bases:

>100_bases
ATGGACGAGGTTAAACACGACTATGGGATCCATATTTAAGCAGTAGATATTCTATTTTTGTCTTTTGTTAGGGTTTCCTT
CCAAATATTTGTATATATTT

Downstream 100 bases:

>100_bases
GTGTAGGATAAGTAGGGAGCATTGTTTACTATAGTTCCCGTGGAACTATAGTAAACGTTTATCGTTTACGCACACACACT
CATATGCCAACCGATTACGA

Product: guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 773; Mature: 772

Protein sequence:

>773_residues
MAIQYKNGRCKTMDDTKDTKDIKETGEAASVLSADEQMIQDGFNALLNDYLKSNHRRKVERITKAFNFANQAHAGVKRRS
GEPYIMHPIAVARIVCREMGLGSTSICSALLHDVVEDTEYTVEDIRDMFGDKIAQIVDGLTKISGGIFGEQASAQAENFR
KLLLTMSDDIRVILIKIADRLHNMRTLGSMLPAKQFKIAGETLYLYAPLAHRLGLFSIKTELEDLSFKYEHPQEYDFISA
KLKATEESRNKLFERFAAPVDEKLKSMGLQYEMRARVKSVYSIWNKMESKGVAFEDIYDIYAVRIIFDPLPGVDEKNQCW
DIYSAITDIYRIRPDRIRDWVSRPKANGYQALHLTVMGPDGQWVEIQIRSRRMDDIAEKGFAAHWKYKESNVEEDTELDK
WIQTITEILESPDPNALDFLDTIKLNLFTSEIFVFTPKGDIKTLPQGATALDFAYALHSDIGNKCIGAKVNHRLVPLSHP
LSSGDQVEVLTSRSQEPQPEWLNFVTTAKARAKIDAVLKRVRKEVAKYGEIKVLDAFKRSELEASTSNLDKLGMYFGFSK
REEFFYAVEKGDVVLPENLKKLLKEKTDNVLFKYVKQALGVASKKVKQPEEEEAKKEKPKYDKKKPYLLKEEAFERNYVI
AECCKPIPGDDSLGFINDDGNVVVHKRSCPIAMRLKSSFGERILNTVWSSHMNASFEATLEVKGIDSIGVLNTITKTISE
DFNVNIMRLLIEAKDGVFEGKIKMKVHDVEDIQKMCVTLSKIQNIKSVGRVAD

Sequences:

>Translated_773_residues
MAIQYKNGRCKTMDDTKDTKDIKETGEAASVLSADEQMIQDGFNALLNDYLKSNHRRKVERITKAFNFANQAHAGVKRRS
GEPYIMHPIAVARIVCREMGLGSTSICSALLHDVVEDTEYTVEDIRDMFGDKIAQIVDGLTKISGGIFGEQASAQAENFR
KLLLTMSDDIRVILIKIADRLHNMRTLGSMLPAKQFKIAGETLYLYAPLAHRLGLFSIKTELEDLSFKYEHPQEYDFISA
KLKATEESRNKLFERFAAPVDEKLKSMGLQYEMRARVKSVYSIWNKMESKGVAFEDIYDIYAVRIIFDPLPGVDEKNQCW
DIYSAITDIYRIRPDRIRDWVSRPKANGYQALHLTVMGPDGQWVEIQIRSRRMDDIAEKGFAAHWKYKESNVEEDTELDK
WIQTITEILESPDPNALDFLDTIKLNLFTSEIFVFTPKGDIKTLPQGATALDFAYALHSDIGNKCIGAKVNHRLVPLSHP
LSSGDQVEVLTSRSQEPQPEWLNFVTTAKARAKIDAVLKRVRKEVAKYGEIKVLDAFKRSELEASTSNLDKLGMYFGFSK
REEFFYAVEKGDVVLPENLKKLLKEKTDNVLFKYVKQALGVASKKVKQPEEEEAKKEKPKYDKKKPYLLKEEAFERNYVI
AECCKPIPGDDSLGFINDDGNVVVHKRSCPIAMRLKSSFGERILNTVWSSHMNASFEATLEVKGIDSIGVLNTITKTISE
DFNVNIMRLLIEAKDGVFEGKIKMKVHDVEDIQKMCVTLSKIQNIKSVGRVAD
>Mature_772_residues
AIQYKNGRCKTMDDTKDTKDIKETGEAASVLSADEQMIQDGFNALLNDYLKSNHRRKVERITKAFNFANQAHAGVKRRSG
EPYIMHPIAVARIVCREMGLGSTSICSALLHDVVEDTEYTVEDIRDMFGDKIAQIVDGLTKISGGIFGEQASAQAENFRK
LLLTMSDDIRVILIKIADRLHNMRTLGSMLPAKQFKIAGETLYLYAPLAHRLGLFSIKTELEDLSFKYEHPQEYDFISAK
LKATEESRNKLFERFAAPVDEKLKSMGLQYEMRARVKSVYSIWNKMESKGVAFEDIYDIYAVRIIFDPLPGVDEKNQCWD
IYSAITDIYRIRPDRIRDWVSRPKANGYQALHLTVMGPDGQWVEIQIRSRRMDDIAEKGFAAHWKYKESNVEEDTELDKW
IQTITEILESPDPNALDFLDTIKLNLFTSEIFVFTPKGDIKTLPQGATALDFAYALHSDIGNKCIGAKVNHRLVPLSHPL
SSGDQVEVLTSRSQEPQPEWLNFVTTAKARAKIDAVLKRVRKEVAKYGEIKVLDAFKRSELEASTSNLDKLGMYFGFSKR
EEFFYAVEKGDVVLPENLKKLLKEKTDNVLFKYVKQALGVASKKVKQPEEEEAKKEKPKYDKKKPYLLKEEAFERNYVIA
ECCKPIPGDDSLGFINDDGNVVVHKRSCPIAMRLKSSFGERILNTVWSSHMNASFEATLEVKGIDSIGVLNTITKTISED
FNVNIMRLLIEAKDGVFEGKIKMKVHDVEDIQKMCVTLSKIQNIKSVGRVAD

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance [H]

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=713, Percent_Identity=36.3253856942497, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1789147, Length=694, Percent_Identity=30.5475504322767, Blast_Score=353, Evalue=3e-98,
Organism=Caenorhabditis elegans, GI25143118, Length=133, Percent_Identity=34.5864661654135, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 87807; Mature: 87676

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIQYKNGRCKTMDDTKDTKDIKETGEAASVLSADEQMIQDGFNALLNDYLKSNHRRKVE
CEEEECCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RITKAFNFANQAHAGVKRRSGEPYIMHPIAVARIVCREMGLGSTSICSALLHDVVEDTEY
HHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCH
TVEDIRDMFGDKIAQIVDGLTKISGGIFGEQASAQAENFRKLLLTMSDDIRVILIKIADR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LHNMRTLGSMLPAKQFKIAGETLYLYAPLAHRLGLFSIKTELEDLSFKYEHPQEYDFISA
HHHHHHHHHHCCCHHHEECCCEEEEEECHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHH
KLKATEESRNKLFERFAAPVDEKLKSMGLQYEMRARVKSVYSIWNKMESKGVAFEDIYDI
HHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
YAVRIIFDPLPGVDEKNQCWDIYSAITDIYRIRPDRIRDWVSRPKANGYQALHLTVMGPD
HHHHHEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEEEECCC
GQWVEIQIRSRRMDDIAEKGFAAHWKYKESNVEEDTELDKWIQTITEILESPDPNALDFL
CCEEEEEEECCCHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHH
DTIKLNLFTSEIFVFTPKGDIKTLPQGATALDFAYALHSDIGNKCIGAKVNHRLVPLSHP
HHHHHEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCEEEECCCC
LSSGDQVEVLTSRSQEPQPEWLNFVTTAKARAKIDAVLKRVRKEVAKYGEIKVLDAFKRS
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHH
ELEASTSNLDKLGMYFGFSKREEFFYAVEKGDVVLPENLKKLLKEKTDNVLFKYVKQALG
HHHHHHCCHHHHHHHHCCCCCCCEEEEECCCCEECCHHHHHHHHHHHHHHHHHHHHHHHH
VASKKVKQPEEEEAKKEKPKYDKKKPYLLKEEAFERNYVIAECCKPIPGDDSLGFINDDG
HHHHHHCCCCHHHHHHCCCCCCCCCCCEEHHHHHCCCCCHHHHCCCCCCCCCCEEEECCC
NVVVHKRSCPIAMRLKSSFGERILNTVWSSHMNASFEATLEVKGIDSIGVLNTITKTISE
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHC
DFNVNIMRLLIEAKDGVFEGKIKMKVHDVEDIQKMCVTLSKIQNIKSVGRVAD
CCCCHHHHHHHHHCCCEEECEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AIQYKNGRCKTMDDTKDTKDIKETGEAASVLSADEQMIQDGFNALLNDYLKSNHRRKVE
EEEECCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RITKAFNFANQAHAGVKRRSGEPYIMHPIAVARIVCREMGLGSTSICSALLHDVVEDTEY
HHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCH
TVEDIRDMFGDKIAQIVDGLTKISGGIFGEQASAQAENFRKLLLTMSDDIRVILIKIADR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LHNMRTLGSMLPAKQFKIAGETLYLYAPLAHRLGLFSIKTELEDLSFKYEHPQEYDFISA
HHHHHHHHHHCCCHHHEECCCEEEEEECHHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHH
KLKATEESRNKLFERFAAPVDEKLKSMGLQYEMRARVKSVYSIWNKMESKGVAFEDIYDI
HHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
YAVRIIFDPLPGVDEKNQCWDIYSAITDIYRIRPDRIRDWVSRPKANGYQALHLTVMGPD
HHHHHEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEEEECCC
GQWVEIQIRSRRMDDIAEKGFAAHWKYKESNVEEDTELDKWIQTITEILESPDPNALDFL
CCEEEEEEECCCHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHH
DTIKLNLFTSEIFVFTPKGDIKTLPQGATALDFAYALHSDIGNKCIGAKVNHRLVPLSHP
HHHHHEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCEEEECCCC
LSSGDQVEVLTSRSQEPQPEWLNFVTTAKARAKIDAVLKRVRKEVAKYGEIKVLDAFKRS
CCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHH
ELEASTSNLDKLGMYFGFSKREEFFYAVEKGDVVLPENLKKLLKEKTDNVLFKYVKQALG
HHHHHHCCHHHHHHHHCCCCCCCEEEEECCCCEECCHHHHHHHHHHHHHHHHHHHHHHHH
VASKKVKQPEEEEAKKEKPKYDKKKPYLLKEEAFERNYVIAECCKPIPGDDSLGFINDDG
HHHHHHCCCCHHHHHHCCCCCCCCCCCEEHHHHHCCCCCHHHHCCCCCCCCCCEEEECCC
NVVVHKRSCPIAMRLKSSFGERILNTVWSSHMNASFEATLEVKGIDSIGVLNTITKTISE
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHC
DFNVNIMRLLIEAKDGVFEGKIKMKVHDVEDIQKMCVTLSKIQNIKSVGRVAD
CCCCHHHHHHHHHCCCEEECEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]