Click here to switch to the map view.

The map label for this gene is miaB

Identifier: 150009019

GI number: 150009019

Start: 2839279

End: 2840652

Strand: Reverse

Name: miaB

Synonym: BDI_2415

Alternate gene names: 150009019

Gene position: 2840652-2839279 (Counterclockwise)

Preceding gene: 150009027

Following gene: 150009015

Centisome position: 59.04

GC content: 45.85

Gene sequence:

>1374_bases
ATGGTAAATCAAGAAAACGGTGTGGACTTAAAATCCGCTACCCAAAGCGATGAACGAAAGTTGTTTATCGAGACCTACGG
CTGTCAGATGAATGTGGCGGACAGCGAAGTTGTAGCTTCTATCATGAAGATGGATGGCTATAGTATGACGGAGAATATCG
AGGAGGCGGATGCCATCTTCGTGAATACTTGCTCCGTACGCGACAACGCCGAACAGAAAATCTATGGGCGGCTACAGTAT
TTCCAGTCTTTAAAACGGAAAAAGAAGTCCTTAATTGTCGGTGTATTGGGATGTATGGCCGAACGAGTGAAGGAAGATCT
GATCCATGTGCATCATGCCGACTTGGTGGTAGGACCGGATTCTTATCTGGACTTGCCGAACTTGGTGGGAGCGGTAGAGC
ACGGAGAGAAAGCGATCAACGTGGAGCTTTCTACCCAAGAGACCTATAAGGATGTTATTCCTTTGAAGTTACCGGGCGTA
CATATCTCCGGTTTCGTGTCTATTATGCGCGGTTGTAATAATTTTTGTACGTATTGCATCGTACCTTATACAAGAGGACG
CGAGCGTAGCCGGGATATCGAGAGTATCTTGAATGAGATACGGGATATGCACGAGAAAGGCTTTAAGGAAGTTACGTTGT
TGGGACAAAATGTAAACTCCTATTCTTTCGAGAAAGAAGGTGAGACCATAACCTTCCCGATCTTGTTGGATCGTGTGGCG
AAAGAGGTTCCCGATATGCGAATTCGTTTTACGACCTCCCATCCGAAGGACATGAGCGATGATACCTTGCGTGTGATTGC
CGCGAACGACAATATCTGTAAGTTTATCCATCTGCCTGCCCAATCCGGCAGCAGTCGCATCTTGAAGGTCATGAATCGTA
AATATACCCGTGAATGGTATCTGGACCGTATAGCGGCGATCCGTCGTATCGTACCGGATTGCGCTATCTCGACTGATTTA
TTCTGCGGTTTCCATTCCGAGACCGAGGAGGATTATCAAGAGACTTTATCCTTGATGCGTGAAGTGGGATATGATAGTGC
TTTCTTATTTAAATATTCCGAACGTCCGGGTACATATGCCGCTAGCCATTTGGAGGATAATGTACCGGAAGAAGAGAAGG
TACGTCGTTTGCAAGGTATGATCGATTTGCAAAATAAGTTGTCCGAGGAAAGTAACTTGCGGGATATAGGCAAGACTTTC
GAGGTTCTGATAGAAGGATTCTCGAAACGTTCCCGTGAGCAATTGTTCGGACGAACCAGCCAAAACAAGGTGGTTATCTT
TGACAAGAAGAACTATCATGTAGGTCAATTCATAAAAGTCAAGATCCATAAGGCTTCCTCGGCAACCCTGTTCGGGGAAC
CTGTGGAGGAATAA

Upstream 100 bases:

>100_bases
GTTTTTCGAATGCAAATATAACGGTGGGATTCGAACTATGCAAGCGTTTGCGTAATTATTTTTAAGAATCTTGCTTATTT
AAATTATATTAACATATAGT

Downstream 100 bases:

>100_bases
GAAAAAGAAAACGCCAATACGTTCCATTTCGAGCGATATTGGCGTTTCTCTTATTATCTTCTATCTTTGGAATATCTCCG
GGTATTTGTTGATGAAATAA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MVNQENGVDLKSATQSDERKLFIETYGCQMNVADSEVVASIMKMDGYSMTENIEEADAIFVNTCSVRDNAEQKIYGRLQY
FQSLKRKKKSLIVGVLGCMAERVKEDLIHVHHADLVVGPDSYLDLPNLVGAVEHGEKAINVELSTQETYKDVIPLKLPGV
HISGFVSIMRGCNNFCTYCIVPYTRGRERSRDIESILNEIRDMHEKGFKEVTLLGQNVNSYSFEKEGETITFPILLDRVA
KEVPDMRIRFTTSHPKDMSDDTLRVIAANDNICKFIHLPAQSGSSRILKVMNRKYTREWYLDRIAAIRRIVPDCAISTDL
FCGFHSETEEDYQETLSLMREVGYDSAFLFKYSERPGTYAASHLEDNVPEEEKVRRLQGMIDLQNKLSEESNLRDIGKTF
EVLIEGFSKRSREQLFGRTSQNKVVIFDKKNYHVGQFIKVKIHKASSATLFGEPVEE

Sequences:

>Translated_457_residues
MVNQENGVDLKSATQSDERKLFIETYGCQMNVADSEVVASIMKMDGYSMTENIEEADAIFVNTCSVRDNAEQKIYGRLQY
FQSLKRKKKSLIVGVLGCMAERVKEDLIHVHHADLVVGPDSYLDLPNLVGAVEHGEKAINVELSTQETYKDVIPLKLPGV
HISGFVSIMRGCNNFCTYCIVPYTRGRERSRDIESILNEIRDMHEKGFKEVTLLGQNVNSYSFEKEGETITFPILLDRVA
KEVPDMRIRFTTSHPKDMSDDTLRVIAANDNICKFIHLPAQSGSSRILKVMNRKYTREWYLDRIAAIRRIVPDCAISTDL
FCGFHSETEEDYQETLSLMREVGYDSAFLFKYSERPGTYAASHLEDNVPEEEKVRRLQGMIDLQNKLSEESNLRDIGKTF
EVLIEGFSKRSREQLFGRTSQNKVVIFDKKNYHVGQFIKVKIHKASSATLFGEPVEE
>Mature_457_residues
MVNQENGVDLKSATQSDERKLFIETYGCQMNVADSEVVASIMKMDGYSMTENIEEADAIFVNTCSVRDNAEQKIYGRLQY
FQSLKRKKKSLIVGVLGCMAERVKEDLIHVHHADLVVGPDSYLDLPNLVGAVEHGEKAINVELSTQETYKDVIPLKLPGV
HISGFVSIMRGCNNFCTYCIVPYTRGRERSRDIESILNEIRDMHEKGFKEVTLLGQNVNSYSFEKEGETITFPILLDRVA
KEVPDMRIRFTTSHPKDMSDDTLRVIAANDNICKFIHLPAQSGSSRILKVMNRKYTREWYLDRIAAIRRIVPDCAISTDL
FCGFHSETEEDYQETLSLMREVGYDSAFLFKYSERPGTYAASHLEDNVPEEEKVRRLQGMIDLQNKLSEESNLRDIGKTF
EVLIEGFSKRSREQLFGRTSQNKVVIFDKKNYHVGQFIKVKIHKASSATLFGEPVEE

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872782, Length=474, Percent_Identity=46.4135021097046, Blast_Score=427, Evalue=1e-119,
Organism=Homo sapiens, GI28872784, Length=474, Percent_Identity=46.8354430379747, Blast_Score=427, Evalue=1e-119,
Organism=Homo sapiens, GI93277076, Length=465, Percent_Identity=26.2365591397849, Blast_Score=148, Evalue=1e-35,
Organism=Escherichia coli, GI1786882, Length=441, Percent_Identity=40.8163265306122, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1787057, Length=456, Percent_Identity=27.8508771929825, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17553146, Length=467, Percent_Identity=43.254817987152, Blast_Score=373, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI71996771, Length=297, Percent_Identity=26.9360269360269, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21356207, Length=478, Percent_Identity=45.1882845188285, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI19922432, Length=451, Percent_Identity=27.9379157427938, Blast_Score=151, Evalue=8e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_PARD8 (A6LEM6)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001303762.1
- ProteinModelPortal:   A6LEM6
- SMR:   A6LEM6
- STRING:   A6LEM6
- GeneID:   5307564
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_2415
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- ProtClustDB:   PRK14329
- BioCyc:   PDIS435591:BDI_2415-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 52150; Mature: 52150

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNQENGVDLKSATQSDERKLFIETYGCQMNVADSEVVASIMKMDGYSMTENIEEADAIF
CCCCCCCCCCHHCCCCCCCEEEEEEECCEEECCHHHHHHHHHHHCCCCHHCCHHHCCEEE
VNTCSVRDNAEQKIYGRLQYFQSLKRKKKSLIVGVLGCMAERVKEDLIHVHHADLVVGPD
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
SYLDLPNLVGAVEHGEKAINVELSTQETYKDVIPLKLPGVHISGFVSIMRGCNNFCTYCI
HHCCCHHHHHHHHCCCEEEEEEECCHHHHHCCCCEECCCCHHHHHHHHHHHHHHCEEEEE
VPYTRGRERSRDIESILNEIRDMHEKGFKEVTLLGQNVNSYSFEKEGETITFPILLDRVA
EECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEHHHHHHHHH
KEVPDMRIRFTTSHPKDMSDDTLRVIAANDNICKFIHLPAQSGSSRILKVMNRKYTREWY
HHCCCCEEEEECCCCCCCCCCCEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
LDRIAAIRRIVPDCAISTDLFCGFHSETEEDYQETLSLMREVGYDSAFLFKYSERPGTYA
HHHHHHHHHHCCCHHCCHHHEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEE
ASHLEDNVPEEEKVRRLQGMIDLQNKLSEESNLRDIGKTFEVLIEGFSKRSREQLFGRTS
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QNKVVIFDKKNYHVGQFIKVKIHKASSATLFGEPVEE
CCEEEEEECCCCCCCCEEEEEEEECCCCEECCCCCCC
>Mature Secondary Structure
MVNQENGVDLKSATQSDERKLFIETYGCQMNVADSEVVASIMKMDGYSMTENIEEADAIF
CCCCCCCCCCHHCCCCCCCEEEEEEECCEEECCHHHHHHHHHHHCCCCHHCCHHHCCEEE
VNTCSVRDNAEQKIYGRLQYFQSLKRKKKSLIVGVLGCMAERVKEDLIHVHHADLVVGPD
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
SYLDLPNLVGAVEHGEKAINVELSTQETYKDVIPLKLPGVHISGFVSIMRGCNNFCTYCI
HHCCCHHHHHHHHCCCEEEEEEECCHHHHHCCCCEECCCCHHHHHHHHHHHHHHCEEEEE
VPYTRGRERSRDIESILNEIRDMHEKGFKEVTLLGQNVNSYSFEKEGETITFPILLDRVA
EECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEHHHHHHHHH
KEVPDMRIRFTTSHPKDMSDDTLRVIAANDNICKFIHLPAQSGSSRILKVMNRKYTREWY
HHCCCCEEEEECCCCCCCCCCCEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
LDRIAAIRRIVPDCAISTDLFCGFHSETEEDYQETLSLMREVGYDSAFLFKYSERPGTYA
HHHHHHHHHHCCCHHCCHHHEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEE
ASHLEDNVPEEEKVRRLQGMIDLQNKLSEESNLRDIGKTFEVLIEGFSKRSREQLFGRTS
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
QNKVVIFDKKNYHVGQFIKVKIHKASSATLFGEPVEE
CCEEEEEECCCCCCCCEEEEEEEECCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA