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The map label for this gene is rpoA

Identifier: 150008958

GI number: 150008958

Start: 2776125

End: 2777117

Strand: Reverse

Name: rpoA

Synonym: BDI_2354

Alternate gene names: 150008958

Gene position: 2777117-2776125 (Counterclockwise)

Preceding gene: 150008959

Following gene: 150008957

Centisome position: 57.72

GC content: 40.79

Gene sequence:

>993_bases
ATGGCGATATTAGCATTTCAAAAACCCGATAAAGTATTAATGTTGGAAGCGGACAATTTCTTCGGAAAGTTTGAATTTCG
TCCGTTGGAACCCGGCTATGGTATTACAATAGGTAATGCTTTACGTCGTATTCTCCTGTCGTCACTTGAAGGTTTTGCTA
TTACATCCATTAAGATCGACGGGGTTGACCATGAATTTGGTACAATCCCGGGTGTTATCGAGGATGTGACAAACATTATC
TTGAATCTGAAGCAAGTTCGTTTCAAGCATATCGTAGACGACATCGAGAATGAAAAAGTGAGTATTACTGTTTCGGGTTC
GGAAGTGTTCAAAGCCGGTGATATTGGTAAGCAACTGACTGGATTCGAGATATTGAATCCTGATTTGGTTATTTGCCGCT
TGGATCCGACCGCAAGTTTCCAGATTGAATTGACTATTAATAAAGGTCGCGGTTATGTACCCGCAGATGAGAACAGAGAC
CCGAACGCAGATATACATGTGATCGCTATCGACTCTATCTTCACTCCGATACGTAACGTAAAATATTCAATCGAGAACTT
CCGTGTTGAACAGAAGACGGACTACGAGAAGTTGGTTCTTGAAATAGCCACAGATGGTTCTATTCATCCGAAGGAAGCGT
TGAAGGAAGCAGCGAAGATTCTGATTCATCATTTCATGTTGTTCTCAGATGAAAAGATTGCGATCGAGACAGCGGATACT
GAAGGCAATGAAGAGTTCGATGAAGAAGTACTTCATATGCGTCAGTTGCTTAAGAGCAAGCTGGCTGATATGGACCTTTC
CGTTCGTGCATTGAATTGCTTAAAGGCCGCAGATGTTGAAACACTAGGTGAGTTGGTTAAGTTCAATAAGAATGATTTGT
TGAAATTCCGTAACTTCGGTAAAAAGTCGCTCACTGAACTTGATGAACTGCTTGAGAGCTTGAACCTTTCATTCGGTATG
GATATCACAAAATATAAATTAGATAAAGAGTAA

Upstream 100 bases:

>100_bases
GAGCGGAAAATTACTGCATCTGCCGGAAAGAGCTGACATTCCTGAAAACATTAAAGAGCAGTTGATCGTAGAATTGTATT
CTAAATAATAATTGATTTCC

Downstream 100 bases:

>100_bases
GTAAACGATGAGACATAATAAGAAATTCAATCATTTAGGTCGTACAAATACTCACCGTGACGCAATGCTTTCGAATATGG
CTTGTTCTTTGATCAAGCAT

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MAILAFQKPDKVLMLEADNFFGKFEFRPLEPGYGITIGNALRRILLSSLEGFAITSIKIDGVDHEFGTIPGVIEDVTNII
LNLKQVRFKHIVDDIENEKVSITVSGSEVFKAGDIGKQLTGFEILNPDLVICRLDPTASFQIELTINKGRGYVPADENRD
PNADIHVIAIDSIFTPIRNVKYSIENFRVEQKTDYEKLVLEIATDGSIHPKEALKEAAKILIHHFMLFSDEKIAIETADT
EGNEEFDEEVLHMRQLLKSKLADMDLSVRALNCLKAADVETLGELVKFNKNDLLKFRNFGKKSLTELDELLESLNLSFGM
DITKYKLDKE

Sequences:

>Translated_330_residues
MAILAFQKPDKVLMLEADNFFGKFEFRPLEPGYGITIGNALRRILLSSLEGFAITSIKIDGVDHEFGTIPGVIEDVTNII
LNLKQVRFKHIVDDIENEKVSITVSGSEVFKAGDIGKQLTGFEILNPDLVICRLDPTASFQIELTINKGRGYVPADENRD
PNADIHVIAIDSIFTPIRNVKYSIENFRVEQKTDYEKLVLEIATDGSIHPKEALKEAAKILIHHFMLFSDEKIAIETADT
EGNEEFDEEVLHMRQLLKSKLADMDLSVRALNCLKAADVETLGELVKFNKNDLLKFRNFGKKSLTELDELLESLNLSFGM
DITKYKLDKE
>Mature_329_residues
AILAFQKPDKVLMLEADNFFGKFEFRPLEPGYGITIGNALRRILLSSLEGFAITSIKIDGVDHEFGTIPGVIEDVTNIIL
NLKQVRFKHIVDDIENEKVSITVSGSEVFKAGDIGKQLTGFEILNPDLVICRLDPTASFQIELTINKGRGYVPADENRDP
NADIHVIAIDSIFTPIRNVKYSIENFRVEQKTDYEKLVLEIATDGSIHPKEALKEAAKILIHHFMLFSDEKIAIETADTE
GNEEFDEEVLHMRQLLKSKLADMDLSVRALNCLKAADVETLGELVKFNKNDLLKFRNFGKKSLTELDELLESLNLSFGMD
ITKYKLDKE

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=328, Percent_Identity=38.4146341463415, Blast_Score=211, Evalue=4e-56,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_PARD8 (A6LEG5)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001303701.1
- ProteinModelPortal:   A6LEG5
- STRING:   A6LEG5
- GeneID:   5307503
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_2354
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   PDIS435591:BDI_2354-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 37190; Mature: 37059

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAILAFQKPDKVLMLEADNFFGKFEFRPLEPGYGITIGNALRRILLSSLEGFAITSIKID
CEEEEECCCCCEEEEECCCCEEEEEEEECCCCCCEEHHHHHHHHHHHHCCCEEEEEEEEE
GVDHEFGTIPGVIEDVTNIILNLKQVRFKHIVDDIENEKVSITVSGSEVFKAGDIGKQLT
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHEECCCCCCCCC
GFEILNPDLVICRLDPTASFQIELTINKGRGYVPADENRDPNADIHVIAIDSIFTPIRNV
CCEEECCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHH
KYSIENFRVEQKTDYEKLVLEIATDGSIHPKEALKEAAKILIHHFMLFSDEKIAIETADT
EEHHHHEEECCCCCHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
EGNEEFDEEVLHMRQLLKSKLADMDLSVRALNCLKAADVETLGELVKFNKNDLLKFRNFG
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHCC
KKSLTELDELLESLNLSFGMDITKYKLDKE
HHHHHHHHHHHHHCCCCCCCCEEEEECCCC
>Mature Secondary Structure 
AILAFQKPDKVLMLEADNFFGKFEFRPLEPGYGITIGNALRRILLSSLEGFAITSIKID
EEEEECCCCCEEEEECCCCEEEEEEEECCCCCCEEHHHHHHHHHHHHCCCEEEEEEEEE
GVDHEFGTIPGVIEDVTNIILNLKQVRFKHIVDDIENEKVSITVSGSEVFKAGDIGKQLT
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHEECCCCCCCCC
GFEILNPDLVICRLDPTASFQIELTINKGRGYVPADENRDPNADIHVIAIDSIFTPIRNV
CCEEECCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHH
KYSIENFRVEQKTDYEKLVLEIATDGSIHPKEALKEAAKILIHHFMLFSDEKIAIETADT
EEHHHHEEECCCCCHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCC
EGNEEFDEEVLHMRQLLKSKLADMDLSVRALNCLKAADVETLGELVKFNKNDLLKFRNFG
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHCC
KKSLTELDELLESLNLSFGMDITKYKLDKE
HHHHHHHHHHHHHCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA