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The map label for this gene is wcaF [H]

Identifier: 150008815

GI number: 150008815

Start: 2594451

End: 2595008

Strand: Reverse

Name: wcaF [H]

Synonym: BDI_2205

Alternate gene names: 150008815

Gene position: 2595008-2594451 (Counterclockwise)

Preceding gene: 150008816

Following gene: 150008814

Centisome position: 53.93

GC content: 39.07

Gene sequence:

>558_bases
ATGGTACAAACAGATCTTTCCAAGTATGATAATTCATGGTTCCATATTGGTGCCAGACGATTGAAAGCTGTCACTTGGTA
TTTTATCAATGCTTTATTTTTAAATTGTAGTTGGAACCCATCATCGGGGCTAAAGGTAAGACTACTACGGATTTTTGGAG
CAGATATAGGTAAGGGAGTTGTAATAAAGCCCGGGGTCAATATTAAATATCCATGGAATTTATCAATCGGTGATTACTCT
TGGATTGGAGAAAATGTATGGATAGACAATTTAGTTCGAGTAACCATCGGTTCCAATGTCTGCATAAGCCAAGGGGCTAT
GTTGTTGTGTGGTAACCATAATTATAAAAGACAAACTTTCGATCTTATAGTAAAATCTATTGTTATTGAAGATGGTGCAT
GGGTCGGTGCCCAGTCCACTGTTTGTCCCGGAGTAACAATGCATTCACATAGTGTTCTTGCTGTCGGTTCCATAGCCTCT
AAAGACTTAATGTCATATTCGATATATCGCGGGAATCCTGCGGTCAAAGTCGCAGAACGGGTAATGATTGAATCGTAA

Upstream 100 bases:

>100_bases
ATTTGCAGATGAGAAGTTGTGTTATAGCTTTTATCAGTGCATATCTTAGCAATGACGAAACCCAAAAACGCTATCTGAAT
ATGTTTCAATTATAATAAGA

Downstream 100 bases:

>100_bases
ACAAACTCTTCACCTTCACCAAAAATTAATTCAATTTAATCTGATGAAAATATCAATAATCACTGCTACATGGAATAGCG
GTGCGACTTTGCGTTACACA

Product: putative colanic acid biosynthesis acetyltransferase WcaF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MVQTDLSKYDNSWFHIGARRLKAVTWYFINALFLNCSWNPSSGLKVRLLRIFGADIGKGVVIKPGVNIKYPWNLSIGDYS
WIGENVWIDNLVRVTIGSNVCISQGAMLLCGNHNYKRQTFDLIVKSIVIEDGAWVGAQSTVCPGVTMHSHSVLAVGSIAS
KDLMSYSIYRGNPAVKVAERVMIES

Sequences:

>Translated_185_residues
MVQTDLSKYDNSWFHIGARRLKAVTWYFINALFLNCSWNPSSGLKVRLLRIFGADIGKGVVIKPGVNIKYPWNLSIGDYS
WIGENVWIDNLVRVTIGSNVCISQGAMLLCGNHNYKRQTFDLIVKSIVIEDGAWVGAQSTVCPGVTMHSHSVLAVGSIAS
KDLMSYSIYRGNPAVKVAERVMIES
>Mature_185_residues
MVQTDLSKYDNSWFHIGARRLKAVTWYFINALFLNCSWNPSSGLKVRLLRIFGADIGKGVVIKPGVNIKYPWNLSIGDYS
WIGENVWIDNLVRVTIGSNVCISQGAMLLCGNHNYKRQTFDLIVKSIVIEDGAWVGAQSTVCPGVTMHSHSVLAVGSIAS
KDLMSYSIYRGNPAVKVAERVMIES

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1788367, Length=182, Percent_Identity=38.4615384615385, Blast_Score=124, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.- [C]

Molecular weight: Translated: 20512; Mature: 20512

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVQTDLSKYDNSWFHIGARRLKAVTWYFINALFLNCSWNPSSGLKVRLLRIFGADIGKGV
CCCCCCHHHCCCEEEECHHHHHHHHHEEEEEEEEEECCCCCCCCEEEEEEEECCCCCCCE
VIKPGVNIKYPWNLSIGDYSWIGENVWIDNLVRVTIGSNVCISQGAMLLCGNHNYKRQTF
EECCCCCEEECEECCCCCCEECCCCEEECCEEEEEECCCEEECCCCEEEECCCCCCHHHH
DLIVKSIVIEDGAWVGAQSTVCPGVTMHSHSVLAVGSIASKDLMSYSIYRGNPAVKVAER
HHHHHHHHEECCCEECCCCCCCCCEEECCCCEEEECCCCHHHHHEEEEEECCCHHHHHHH
VMIES
HCCCC
>Mature Secondary Structure
MVQTDLSKYDNSWFHIGARRLKAVTWYFINALFLNCSWNPSSGLKVRLLRIFGADIGKGV
CCCCCCHHHCCCEEEECHHHHHHHHHEEEEEEEEEECCCCCCCCEEEEEEEECCCCCCCE
VIKPGVNIKYPWNLSIGDYSWIGENVWIDNLVRVTIGSNVCISQGAMLLCGNHNYKRQTF
EECCCCCEEECEECCCCCCEECCCCEEECCEEEEEECCCEEECCCCEEEECCCCCCHHHH
DLIVKSIVIEDGAWVGAQSTVCPGVTMHSHSVLAVGSIASKDLMSYSIYRGNPAVKVAER
HHHHHHHHEECCCEECCCCCCCCCEEECCCCEEEECCCCHHHHHEEEEEECCCHHHHHHH
VMIES
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]