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The map label for this gene is trmE

Identifier: 150008732

GI number: 150008732

Start: 2503465

End: 2504850

Strand: Direct

Name: trmE

Synonym: BDI_2122

Alternate gene names: 150008732

Gene position: 2503465-2504850 (Clockwise)

Preceding gene: 150008731

Following gene: 150008733

Centisome position: 52.03

GC content: 48.85

Gene sequence:

>1386_bases
ATGAGTACAATTTGCGCTATATCTACAGCTCCGGGAGTAGGAGGAATCGCAGTGATTCGTGTCTCCGGACCGGATACTTT
CAAGATTTGTGATCGGATATTCCGGCCGAAGAAGGCAGGTAAAAGTTTATCTACCCAAAAGGCTTATACCTTAACGTATG
GTTCCATCGTCGGTAACAATGACGAAACCATAGATGAGGTGATCGCCGCTGTTTTCCGTGCCCCCCATTCTTTCACGGGG
GAAGATACGGTGGAAATCACCTGCCACGGCTCGACGTATATCCAACAACAAATCCTGCAATCCCTTATCTCCAGCGGATG
CCGTATAGCGCAACCGGGTGAATATACGCAACGTGCTTTCATGAATGGCAAGATGGACCTTAGCCAAGCGGAGGCCGTTG
CGGATTTAATCGCCTCCACTTCGGCCGGCCAACATCGTTTGGCTCTAAGCCAGATGCGAGGTGGTTTCAGCAGGGAACTC
GCCGAACTTCGAAACCAACTGCTCCATTTCACCTCTCTCATGGAATTGGAACTGGATTTCAGTGACCATGAGGAATTAGA
GTTCGCTGATCGTAGCGAGCTTCGCACGCTTGCGGATCATATCGAACAGGTCATCTCCAAACTCGCCCAATCTTTCAGCG
TAGGTAACGCTATCAAAAACGGTATTCCTGTCGCCATCATCGGTGAGACGAACGCCGGCAAATCTACTTTACTAAACGCC
TTGTTGAACGAGGATAAAGCGATCGTCAGCGACATCCACGGAACGACCCGTGACGTTATCGAGGATACGATCAATATCAA
CGGCCAACTCTTCCGTTTCATCGACACCGCCGGTATCCGTGAAACAAGCGATGCGATCGAAGCTCTCGGTATTGAACGTA
GTTTCAAGGCTCTAGACCAAGCGCAAATCGTCATCCTCATGTACGACTTAACCCGTGACCTCAAGGATTTCGAGGCTTTC
TATCAAGAAATAGCTCCGAGATTAACAAATAAGTCTGTTATCTTAGCGATGAACAAATGCGATGTATTACCCACATCCTC
TCTCCCAACCTTCTCTTTCCCCACCGAAGGCTGGCATCAAATCGCTATTTCCGCCAAGAGCAAGCTGCATATCGCAGAGC
TACAACAACTCCTCACGGAAGTCTCTTCCATTCCCACCCTCCACCAAAGCGACATCATCGTAACGAATGTCCGCCACTTC
GAGGCACTTACGCATGCGCTGGAAGCTATACATAGGGTACAAGAAGGTTTGAACTCCAGCCTTTCCGGGGATTTCATCTC
GCAAGATTTACGAGAGTGTATATTCCATTTATCGGATATCGTAGGAGAGGTTACGACAGATCAGGTGCTGGGGAATATAT
TTCGGCATTTTTGCATCGGCAAGTAA

Upstream 100 bases:

>100_bases
TGATTCATCCGGATACGAACTGGCAAGCCGGCCTACGTTATGCGGAAGAAATCGGACTGGGTTCTCAAGCGTATGAATTA
ATAACGGTTTAATAGACGGA

Downstream 100 bases:

>100_bases
ATACCTGATTATTAAATAGTTAGATTGATTATAATCGATTAATATGGCGCTCTTTACGGGCGCCTTTTTTGTTGCTCAAG
TATCGTTGATAATCGTTGCT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MSTICAISTAPGVGGIAVIRVSGPDTFKICDRIFRPKKAGKSLSTQKAYTLTYGSIVGNNDETIDEVIAAVFRAPHSFTG
EDTVEITCHGSTYIQQQILQSLISSGCRIAQPGEYTQRAFMNGKMDLSQAEAVADLIASTSAGQHRLALSQMRGGFSREL
AELRNQLLHFTSLMELELDFSDHEELEFADRSELRTLADHIEQVISKLAQSFSVGNAIKNGIPVAIIGETNAGKSTLLNA
LLNEDKAIVSDIHGTTRDVIEDTININGQLFRFIDTAGIRETSDAIEALGIERSFKALDQAQIVILMYDLTRDLKDFEAF
YQEIAPRLTNKSVILAMNKCDVLPTSSLPTFSFPTEGWHQIAISAKSKLHIAELQQLLTEVSSIPTLHQSDIIVTNVRHF
EALTHALEAIHRVQEGLNSSLSGDFISQDLRECIFHLSDIVGEVTTDQVLGNIFRHFCIGK

Sequences:

>Translated_461_residues
MSTICAISTAPGVGGIAVIRVSGPDTFKICDRIFRPKKAGKSLSTQKAYTLTYGSIVGNNDETIDEVIAAVFRAPHSFTG
EDTVEITCHGSTYIQQQILQSLISSGCRIAQPGEYTQRAFMNGKMDLSQAEAVADLIASTSAGQHRLALSQMRGGFSREL
AELRNQLLHFTSLMELELDFSDHEELEFADRSELRTLADHIEQVISKLAQSFSVGNAIKNGIPVAIIGETNAGKSTLLNA
LLNEDKAIVSDIHGTTRDVIEDTININGQLFRFIDTAGIRETSDAIEALGIERSFKALDQAQIVILMYDLTRDLKDFEAF
YQEIAPRLTNKSVILAMNKCDVLPTSSLPTFSFPTEGWHQIAISAKSKLHIAELQQLLTEVSSIPTLHQSDIIVTNVRHF
EALTHALEAIHRVQEGLNSSLSGDFISQDLRECIFHLSDIVGEVTTDQVLGNIFRHFCIGK
>Mature_460_residues
STICAISTAPGVGGIAVIRVSGPDTFKICDRIFRPKKAGKSLSTQKAYTLTYGSIVGNNDETIDEVIAAVFRAPHSFTGE
DTVEITCHGSTYIQQQILQSLISSGCRIAQPGEYTQRAFMNGKMDLSQAEAVADLIASTSAGQHRLALSQMRGGFSRELA
ELRNQLLHFTSLMELELDFSDHEELEFADRSELRTLADHIEQVISKLAQSFSVGNAIKNGIPVAIIGETNAGKSTLLNAL
LNEDKAIVSDIHGTTRDVIEDTININGQLFRFIDTAGIRETSDAIEALGIERSFKALDQAQIVILMYDLTRDLKDFEAFY
QEIAPRLTNKSVILAMNKCDVLPTSSLPTFSFPTEGWHQIAISAKSKLHIAELQQLLTEVSSIPTLHQSDIIVTNVRHFE
ALTHALEAIHRVQEGLNSSLSGDFISQDLRECIFHLSDIVGEVTTDQVLGNIFRHFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=471, Percent_Identity=29.723991507431, Blast_Score=178, Evalue=9e-45,
Organism=Homo sapiens, GI193082993, Length=462, Percent_Identity=29.004329004329, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI193082988, Length=507, Percent_Identity=28.4023668639053, Blast_Score=166, Evalue=5e-41,
Organism=Escherichia coli, GI2367268, Length=462, Percent_Identity=35.4978354978355, Blast_Score=261, Evalue=8e-71,
Organism=Escherichia coli, GI87082120, Length=129, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17507259, Length=470, Percent_Identity=30.8510638297872, Blast_Score=190, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6323665, Length=500, Percent_Identity=30.8, Blast_Score=183, Evalue=6e-47,
Organism=Drosophila melanogaster, GI45550813, Length=496, Percent_Identity=30.8467741935484, Blast_Score=191, Evalue=8e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_PARD8 (A6LDT9)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001303475.1
- STRING:   A6LDT9
- GeneID:   5307271
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_2122
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   PDIS435591:BDI_2122-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 50637; Mature: 50506

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: BINDING 20-20 BINDING 85-85 BINDING 124-124 BINDING 461-461

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTICAISTAPGVGGIAVIRVSGPDTFKICDRIFRPKKAGKSLSTQKAYTLTYGSIVGNN
CCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHCCHHCCCCCCCCCEEEEEECCEECCC
DETIDEVIAAVFRAPHSFTGEDTVEITCHGSTYIQQQILQSLISSGCRIAQPGEYTQRAF
CHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
MNGKMDLSQAEAVADLIASTSAGQHRLALSQMRGGFSRELAELRNQLLHFTSLMELELDF
HCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECC
SDHEELEFADRSELRTLADHIEQVISKLAQSFSVGNAIKNGIPVAIIGETNAGKSTLLNA
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHH
LLNEDKAIVSDIHGTTRDVIEDTININGQLFRFIDTAGIRETSDAIEALGIERSFKALDQ
HHCCCHHHHHHHCCCHHHHHHHHHCCCCCEEEEHHHCCCCCHHHHHHHHCCHHHHHHHCC
AQIVILMYDLTRDLKDFEAFYQEIAPRLTNKSVILAMNKCDVLPTSSLPTFSFPTEGWHQ
CEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHE
IAISAKSKLHIAELQQLLTEVSSIPTLHQSDIIVTNVRHFEALTHALEAIHRVQEGLNSS
EEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCC
LSGDFISQDLRECIFHLSDIVGEVTTDQVLGNIFRHFCIGK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STICAISTAPGVGGIAVIRVSGPDTFKICDRIFRPKKAGKSLSTQKAYTLTYGSIVGNN
CCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHCCHHCCCCCCCCCEEEEEECCEECCC
DETIDEVIAAVFRAPHSFTGEDTVEITCHGSTYIQQQILQSLISSGCRIAQPGEYTQRAF
CHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
MNGKMDLSQAEAVADLIASTSAGQHRLALSQMRGGFSRELAELRNQLLHFTSLMELELDF
HCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECC
SDHEELEFADRSELRTLADHIEQVISKLAQSFSVGNAIKNGIPVAIIGETNAGKSTLLNA
CCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHH
LLNEDKAIVSDIHGTTRDVIEDTININGQLFRFIDTAGIRETSDAIEALGIERSFKALDQ
HHCCCHHHHHHHCCCHHHHHHHHHCCCCCEEEEHHHCCCCCHHHHHHHHCCHHHHHHHCC
AQIVILMYDLTRDLKDFEAFYQEIAPRLTNKSVILAMNKCDVLPTSSLPTFSFPTEGWHQ
CEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHE
IAISAKSKLHIAELQQLLTEVSSIPTLHQSDIIVTNVRHFEALTHALEAIHRVQEGLNSS
EEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCC
LSGDFISQDLRECIFHLSDIVGEVTTDQVLGNIFRHFCIGK
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA