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The map label for this gene is nfrA1 [H]

Identifier: 150008729

GI number: 150008729

Start: 2500308

End: 2501054

Strand: Reverse

Name: nfrA1 [H]

Synonym: BDI_2119

Alternate gene names: 150008729

Gene position: 2501054-2500308 (Counterclockwise)

Preceding gene: 150008730

Following gene: 150008723

Centisome position: 51.98

GC content: 46.72

Gene sequence:

>747_bases
ATGTTAGAATGCATGAAGAATAGAAGAAGTATCCGTAAATATACGGATCAGGATATCCCGGAGTCTTTGCTGAATGAATT
ATTGGAAGTGGCTACTCGTGCCTCGAATACAGGAAATATGCAATTGTATAGTGTCGTGGTTACCCGCGACCCTGCGAACA
AGGAGAAACTGGCTCCGGCTCATTTTAACCAGCCGATGATTACGGGGGCGCCTGTCGTACTTACTTTTTGTGCGGACGCG
AACCGTTTTGTTAAATGGGCGGAGTGCAGGAAAGCGGAGGCCGGATTCGACAATTTGCAAACGTTTATCGCTTCGACGAT
AGATGCGATGTTGTTCGCCCAGAGTTTCTGTAATGCCGCTGAGGAAAAAGGCTTAGGCATTTGCTATCTGGGTACGACGG
CTTATAATGCGGACCAGATCATTGAGGCGCTTTCTCTTCCCCGTCTGGTTGTCCCGATCGTTACGGTTACGGTGGGTTAT
CCGGCAGAGCCTATACCCGCTCAAGTAGAACGTTTACCATTGGCAGCTGTGGTACAAAACGAGACTTATACGGACTTTAC
TCCAGCTTCCATCGATGCCTTGTATGGCGAGAAAGAGGCATTAGAGGTGAATAAACAGTTTGTGAGGGAGAATAATAAGG
AAACACTGGCGCAGGTCTTTACGGACGTACGCTATACGAAGAAGAATAGTGAATATTTCTCCGAGGTGCTTTTGAAGGTA
TTGAAACAACAAGGATTCATGAAGTAA

Upstream 100 bases:

>100_bases
CGCTTATGCGGCTATCTACGGATTACCGAAAAGCGTGTTTAACCGTATGCCGGCATGGATATTAGGATTTAGTTATTGAG
AATTTAAAAATAGGATTGCT

Downstream 100 bases:

>100_bases
GTCCCAGTACTTCTACCTTATAGTTGATAAAGAGTCCACTCTCGAGTACGCCTGTAATCGACTTGAGGGTGGATTCTGTA
TTTTCAGGGATGTAGTCCAT

Product: putative oxidoreductase

Products: NA

Alternate protein names: NADPH-dependent FMN reductase; NADPH-dependent nitroreductase; NADPH-dependent oxidoreductase [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MLECMKNRRSIRKYTDQDIPESLLNELLEVATRASNTGNMQLYSVVVTRDPANKEKLAPAHFNQPMITGAPVVLTFCADA
NRFVKWAECRKAEAGFDNLQTFIASTIDAMLFAQSFCNAAEEKGLGICYLGTTAYNADQIIEALSLPRLVVPIVTVTVGY
PAEPIPAQVERLPLAAVVQNETYTDFTPASIDALYGEKEALEVNKQFVRENNKETLAQVFTDVRYTKKNSEYFSEVLLKV
LKQQGFMK

Sequences:

>Translated_248_residues
MLECMKNRRSIRKYTDQDIPESLLNELLEVATRASNTGNMQLYSVVVTRDPANKEKLAPAHFNQPMITGAPVVLTFCADA
NRFVKWAECRKAEAGFDNLQTFIASTIDAMLFAQSFCNAAEEKGLGICYLGTTAYNADQIIEALSLPRLVVPIVTVTVGY
PAEPIPAQVERLPLAAVVQNETYTDFTPASIDALYGEKEALEVNKQFVRENNKETLAQVFTDVRYTKKNSEYFSEVLLKV
LKQQGFMK
>Mature_248_residues
MLECMKNRRSIRKYTDQDIPESLLNELLEVATRASNTGNMQLYSVVVTRDPANKEKLAPAHFNQPMITGAPVVLTFCADA
NRFVKWAECRKAEAGFDNLQTFIASTIDAMLFAQSFCNAAEEKGLGICYLGTTAYNADQIIEALSLPRLVVPIVTVTVGY
PAEPIPAQVERLPLAAVVQNETYTDFTPASIDALYGEKEALEVNKQFVRENNKETLAQVFTDVRYTKKNSEYFSEVLLKV
LKQQGFMK

Specific function: Reduces FMNH(2) to FMN, with NADPH as reductant. It also reduces nitroaromatic compounds, quinones and azo dyes [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=245, Percent_Identity=28.1632653061224, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 27720; Mature: 27720

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLECMKNRRSIRKYTDQDIPESLLNELLEVATRASNTGNMQLYSVVVTRDPANKEKLAPA
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCC
HFNQPMITGAPVVLTFCADANRFVKWAECRKAEAGFDNLQTFIASTIDAMLFAQSFCNAA
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
EEKGLGICYLGTTAYNADQIIEALSLPRLVVPIVTVTVGYPAEPIPAQVERLPLAAVVQN
HHCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHCCC
ETYTDFTPASIDALYGEKEALEVNKQFVRENNKETLAQVFTDVRYTKKNSEYFSEVLLKV
CCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LKQQGFMK
HHHCCCCC
>Mature Secondary Structure
MLECMKNRRSIRKYTDQDIPESLLNELLEVATRASNTGNMQLYSVVVTRDPANKEKLAPA
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCC
HFNQPMITGAPVVLTFCADANRFVKWAECRKAEAGFDNLQTFIASTIDAMLFAQSFCNAA
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
EEKGLGICYLGTTAYNADQIIEALSLPRLVVPIVTVTVGYPAEPIPAQVERLPLAAVVQN
HHCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHCCC
ETYTDFTPASIDALYGEKEALEVNKQFVRENNKETLAQVFTDVRYTKKNSEYFSEVLLKV
CCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LKQQGFMK
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 9836433 [H]