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The map label for this gene is miaA

Identifier: 150008721

GI number: 150008721

Start: 2493432

End: 2494331

Strand: Direct

Name: miaA

Synonym: BDI_2111

Alternate gene names: 150008721

Gene position: 2493432-2494331 (Clockwise)

Preceding gene: 150008720

Following gene: 150008724

Centisome position: 51.82

GC content: 48.22

Gene sequence:

>900_bases
ATGAATTCCCTCGTTATATTACTTGGGCCGACAGGAGTCGGAAAGACCGAGTTAAGCCTCCAAGTGGCGGAACGTTTCGG
CTCTCCGATTATCTCTTCCGACTCCCGTCAGCTCTATAAAGACCTTCCGATCGGCACGGCTGCCCCTACACCGGAACAAA
TGGCACGTGTCAAGCATTATATGGTAGGCACTCTCTCGCTTACCGATTATTACAGCGCCAGCAACTTCGAGGAAGATGTG
GTCTCCCTCCTATCCGAATTACATAAAACGATCCCGACCGTCGTAATGACCGGAGGCTCTATGATGTACATCGACGCCGT
ATGTAAAGGTATTGACGATATCCCGACGGTTACACCGGAGATCCGGGATGCCCTATACATGCAATTTGAAACCGAGGGAC
TAGCCCCTATCCTCGCTGAATTAAAAGAAGCAGACCCCGTTCATTACGAGGAAGTGGACCGGAATAATTACAAACGAGTG
ATCCATGCCGTAGAGATTTGCCGGATGACAGGCAAGCCTTACTCCTCCTTCCGCACGAACATTAAGAAAGAGCGTCCATT
CCGGATCATCAAAGTAGGGCTTAATAGAGATCGTGACGAATTATGCGACCGCATCAACCAACGTGTAGATCAAATGATGA
GCGAAGGATTATTGGAAGAGGCTCGCCGGGCTTATCCCTTCCGTCATTTGAATTCCTTGAACACGGTAGGTTATAAAGAA
TTATTCAATTACTTCAGCGGAGAATGGACATTAGACCTCGCCGTGGAGAAAATCAAACGAAACTCCCGGGTCTACGCCCG
GAAGCAAATGACTTGGTTTAAACGGGACCCAGAAATAACATGGTTCCATCCGGATGAGACCGAAGCTATATTTACACATC
TGAGCCAACAGATCATATAG

Upstream 100 bases:

>100_bases
ACGGGGATTCCGAATACGATATGGACGAATTAGACAAAAGCGGCTTCGACGGTTTAGGCGAAGGCCCGATGGATAATCCT
TACGACGACGAGCGCTTTTG

Downstream 100 bases:

>100_bases
CGTTTTAACAATGCCCATTATAAGGTGCTTACAATGGGCATTATAAACATGCTTGAATAGGCATTGTAAGCATCTTACAA
TAGGCATCAAGGTTTTCCGC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase 2; DMAPP:tRNA dimethylallyltransferase 2; DMATase 2; Isopentenyl-diphosphate:tRNA isopentenyltransferase 2; IPP transferase 2; IPPT 2; IPTase 2

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MNSLVILLGPTGVGKTELSLQVAERFGSPIISSDSRQLYKDLPIGTAAPTPEQMARVKHYMVGTLSLTDYYSASNFEEDV
VSLLSELHKTIPTVVMTGGSMMYIDAVCKGIDDIPTVTPEIRDALYMQFETEGLAPILAELKEADPVHYEEVDRNNYKRV
IHAVEICRMTGKPYSSFRTNIKKERPFRIIKVGLNRDRDELCDRINQRVDQMMSEGLLEEARRAYPFRHLNSLNTVGYKE
LFNYFSGEWTLDLAVEKIKRNSRVYARKQMTWFKRDPEITWFHPDETEAIFTHLSQQII

Sequences:

>Translated_299_residues
MNSLVILLGPTGVGKTELSLQVAERFGSPIISSDSRQLYKDLPIGTAAPTPEQMARVKHYMVGTLSLTDYYSASNFEEDV
VSLLSELHKTIPTVVMTGGSMMYIDAVCKGIDDIPTVTPEIRDALYMQFETEGLAPILAELKEADPVHYEEVDRNNYKRV
IHAVEICRMTGKPYSSFRTNIKKERPFRIIKVGLNRDRDELCDRINQRVDQMMSEGLLEEARRAYPFRHLNSLNTVGYKE
LFNYFSGEWTLDLAVEKIKRNSRVYARKQMTWFKRDPEITWFHPDETEAIFTHLSQQII
>Mature_299_residues
MNSLVILLGPTGVGKTELSLQVAERFGSPIISSDSRQLYKDLPIGTAAPTPEQMARVKHYMVGTLSLTDYYSASNFEEDV
VSLLSELHKTIPTVVMTGGSMMYIDAVCKGIDDIPTVTPEIRDALYMQFETEGLAPILAELKEADPVHYEEVDRNNYKRV
IHAVEICRMTGKPYSSFRTNIKKERPFRIIKVGLNRDRDELCDRINQRVDQMMSEGLLEEARRAYPFRHLNSLNTVGYKE
LFNYFSGEWTLDLAVEKIKRNSRVYARKQMTWFKRDPEITWFHPDETEAIFTHLSQQII

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=307, Percent_Identity=25.7328990228013, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1790613, Length=289, Percent_Identity=26.9896193771626, Blast_Score=143, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI25144712, Length=304, Percent_Identity=24.6710526315789, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6324848, Length=288, Percent_Identity=26.3888888888889, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24649830, Length=268, Percent_Identity=29.8507462686567, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA2_PARD8 (A6LDS8)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001303464.1
- ProteinModelPortal:   A6LDS8
- SMR:   A6LDS8
- STRING:   A6LDS8
- GeneID:   5307260
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_2111
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   KEVRHAI
- ProtClustDB:   PRK00091
- BioCyc:   PDIS435591:BDI_2111-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34381; Mature: 34381

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLVILLGPTGVGKTELSLQVAERFGSPIISSDSRQLYKDLPIGTAAPTPEQMARVKHY
CCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHH
MVGTLSLTDYYSASNFEEDVVSLLSELHKTIPTVVMTGGSMMYIDAVCKGIDDIPTVTPE
HHHEEEHHHHHCCCCHHHHHHHHHHHHHHHCCCEEECCCCEEEHHHHHCCCCCCCCCCHH
IRDALYMQFETEGLAPILAELKEADPVHYEEVDRNNYKRVIHAVEICRMTGKPYSSFRTN
HHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHC
IKKERPFRIIKVGLNRDRDELCDRINQRVDQMMSEGLLEEARRAYPFRHLNSLNTVGYKE
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHH
LFNYFSGEWTLDLAVEKIKRNSRVYARKQMTWFKRDPEITWFHPDETEAIFTHLSQQII
HHHHHCCCEEHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNSLVILLGPTGVGKTELSLQVAERFGSPIISSDSRQLYKDLPIGTAAPTPEQMARVKHY
CCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHH
MVGTLSLTDYYSASNFEEDVVSLLSELHKTIPTVVMTGGSMMYIDAVCKGIDDIPTVTPE
HHHEEEHHHHHCCCCHHHHHHHHHHHHHHHCCCEEECCCCEEEHHHHHCCCCCCCCCCHH
IRDALYMQFETEGLAPILAELKEADPVHYEEVDRNNYKRVIHAVEICRMTGKPYSSFRTN
HHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHC
IKKERPFRIIKVGLNRDRDELCDRINQRVDQMMSEGLLEEARRAYPFRHLNSLNTVGYKE
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHH
LFNYFSGEWTLDLAVEKIKRNSRVYARKQMTWFKRDPEITWFHPDETEAIFTHLSQQII
HHHHHCCCEEHHHHHHHHHHCCHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA