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The map label for this gene is truA

Identifier: 150008550

GI number: 150008550

Start: 2293988

End: 2294746

Strand: Reverse

Name: truA

Synonym: BDI_1936

Alternate gene names: 150008550

Gene position: 2294746-2293988 (Counterclockwise)

Preceding gene: 150008555

Following gene: 150008549

Centisome position: 47.69

GC content: 49.54

Gene sequence:

>759_bases
GTGAGACGATATTTTATATATCTGGGATATAACGGAAAGAATTTTCGGGGCTGGCAGATCCAGCCGAATGGTATGACGGT
GCAGCAATCTATAGAGGAGGCCTTGGCCATCTTGATGCGTACGCCCGTGCCTATTGTGGGGGCCGGCCGTACCGATGCCG
GCGTACATGCCCACTTGATGATCGCACATTTTGATTGGGAGGAACCGATCGGGGATTTGGTATTCCTCGCGGAAAAATTG
AATAGGCTCCTGCCCAAAGATATCGCCGTTTATAAGATCGTGCCGGTCGTACCGGAGGCGCATGCCCGCTTCGACGCTAC
CTCGCGAACCTATAAATATTATGTTACGACAAAGAAAGACCCGTTTAATCACGAGCTGGTTTATAAGCTTCCCGGTCGTT
TGGATTTCGAGGCGATGAACGAGGCGTGTAAGGTCTTATTCGATTATATCGATTTTACGAGTTTCAGTAAGTTGCATACG
GATGTAAAGACGAATAATTGCCATATCCAACATGCGGGTTGGACGCAAGAAGGGGATATTTGGGTGTTTACGATCCGTGC
AGACCGTTTCCTTCGTAATATGGTGCGTGCGATCGTTGGTACTTTATTGGAAGTCGGAAGAGGACGTTTAACGATAGACG
GCTTCCGAAACGTGATTGAGGCAAAGGATCGTTGCAAGGCCGGAACCTCCGTGCCGGGACACGCCCTGTTCTTGGTAGAC
GTCACGTACCCGGAAGAACTCTTTCCAGTGGAGGATTAA

Upstream 100 bases:

>100_bases
GATGCAAAATTAAACTTTTTAGCCCATTCGGGCAACGGCTTGGCAGGATTTCTGTACCTTTGTGCCATATTTTAGAAAAG
AGAAAGTAAAGAGCATAAGC

Downstream 100 bases:

>100_bases
ACCATGTGGTTAGCTCTAGCCTTCCTCTCCGCCTTCTTGTTAGGTTGTTATGAGGTCAATAAGAAAATGTCGTTAAATGG
GAATGCCGTTATTCCCGTTT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MRRYFIYLGYNGKNFRGWQIQPNGMTVQQSIEEALAILMRTPVPIVGAGRTDAGVHAHLMIAHFDWEEPIGDLVFLAEKL
NRLLPKDIAVYKIVPVVPEAHARFDATSRTYKYYVTTKKDPFNHELVYKLPGRLDFEAMNEACKVLFDYIDFTSFSKLHT
DVKTNNCHIQHAGWTQEGDIWVFTIRADRFLRNMVRAIVGTLLEVGRGRLTIDGFRNVIEAKDRCKAGTSVPGHALFLVD
VTYPEELFPVED

Sequences:

>Translated_252_residues
MRRYFIYLGYNGKNFRGWQIQPNGMTVQQSIEEALAILMRTPVPIVGAGRTDAGVHAHLMIAHFDWEEPIGDLVFLAEKL
NRLLPKDIAVYKIVPVVPEAHARFDATSRTYKYYVTTKKDPFNHELVYKLPGRLDFEAMNEACKVLFDYIDFTSFSKLHT
DVKTNNCHIQHAGWTQEGDIWVFTIRADRFLRNMVRAIVGTLLEVGRGRLTIDGFRNVIEAKDRCKAGTSVPGHALFLVD
VTYPEELFPVED
>Mature_252_residues
MRRYFIYLGYNGKNFRGWQIQPNGMTVQQSIEEALAILMRTPVPIVGAGRTDAGVHAHLMIAHFDWEEPIGDLVFLAEKL
NRLLPKDIAVYKIVPVVPEAHARFDATSRTYKYYVTTKKDPFNHELVYKLPGRLDFEAMNEACKVLFDYIDFTSFSKLHT
DVKTNNCHIQHAGWTQEGDIWVFTIRADRFLRNMVRAIVGTLLEVGRGRLTIDGFRNVIEAKDRCKAGTSVPGHALFLVD
VTYPEELFPVED

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=275, Percent_Identity=32, Blast_Score=88, Evalue=6e-18,
Organism=Escherichia coli, GI1788657, Length=252, Percent_Identity=34.9206349206349, Blast_Score=134, Evalue=6e-33,
Organism=Drosophila melanogaster, GI19922728, Length=236, Percent_Identity=29.2372881355932, Blast_Score=74, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_PARD8 (A6LDA7)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001303293.1
- ProteinModelPortal:   A6LDA7
- SMR:   A6LDA7
- STRING:   A6LDA7
- GeneID:   5307086
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_1936
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BioCyc:   PDIS435591:BDI_1936-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 28850; Mature: 28850

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRYFIYLGYNGKNFRGWQIQPNGMTVQQSIEEALAILMRTPVPIVGAGRTDAGVHAHLM
CCEEEEEEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEE
IAHFDWEEPIGDLVFLAEKLNRLLPKDIAVYKIVPVVPEAHARFDATSRTYKYYVTTKKD
EEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHCCCCCCCEEEEEEEECCC
PFNHELVYKLPGRLDFEAMNEACKVLFDYIDFTSFSKLHTDVKTNNCHIQHAGWTQEGDI
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCE
WVFTIRADRFLRNMVRAIVGTLLEVGRGRLTIDGFRNVIEAKDRCKAGTSVPGHALFLVD
EEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
VTYPEELFPVED
ECCCHHHCCCCC
>Mature Secondary Structure
MRRYFIYLGYNGKNFRGWQIQPNGMTVQQSIEEALAILMRTPVPIVGAGRTDAGVHAHLM
CCEEEEEEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEE
IAHFDWEEPIGDLVFLAEKLNRLLPKDIAVYKIVPVVPEAHARFDATSRTYKYYVTTKKD
EEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHCCCCCCCEEEEEEEECCC
PFNHELVYKLPGRLDFEAMNEACKVLFDYIDFTSFSKLHTDVKTNNCHIQHAGWTQEGDI
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCE
WVFTIRADRFLRNMVRAIVGTLLEVGRGRLTIDGFRNVIEAKDRCKAGTSVPGHALFLVD
EEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
VTYPEELFPVED
ECCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA