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The map label for this gene is purH [H]

Identifier: 150008499

GI number: 150008499

Start: 2241192

End: 2242715

Strand: Reverse

Name: purH [H]

Synonym: BDI_1885

Alternate gene names: 150008499

Gene position: 2242715-2241192 (Counterclockwise)

Preceding gene: 150008500

Following gene: 150008498

Centisome position: 46.61

GC content: 48.56

Gene sequence:

>1524_bases
ATGGCTGCAACGAAACGAATGAAACGTGCCTTGGTCTCTGTGTTTCACAAAGACGGGCTGGACGAAATCCTAAAGAAATT
GCATAGCGAGGGTGTGAGCTTTGTATCAACAGGTGGTACTCAATCATTTATCGAGTCATTGGGTTTGCCTTGTGATGCGG
TAGAGGACTTGACTTCTTATCCGTCTATTTTGGGCGGTCGTGTGAAGACGCTTCATCCGAAGGTGTTCGGTGGTATCTTG
GCTCGCCGCGAGAACGAGAATGACCAGAAACAATTAGCCGAATATGAGATCCCGGAGATCGACTTGGTTATCGTTGACCT
ATATCCTTTCGAGGAGACTGTCGCTTCCGGCGCTGAGGAACAAGCGATTATTGAGAAAATAGATATAGGCGGTATCTCTT
TGATACGTGCCGCCGCGAAGAACTTCAAGGATGTGGTAATCGTAGCTTCCAAGGCTCAATACCAGCCGTTGATGGATCTT
TTGAACGAGAAGGGCGCTGAGACAAGCATCGAGGATCGTAGATGGTTCGCCAAAGAGGCGTTCGCCGTTTCCTCGGGTTA
CGACTCCGCTATCTTCAATTATTTTGATGGCCGCGAAGGTTCCCATTTCCGTGCAACGGTAGATAGTCCGATGCATTTGC
GTTATGGCGAGAATCCTCATCAAGCCGCTAAGTTCTATGGTAAGTTTAACGATATGTTCGATCAAATCCATGGAAAAGAG
ATTTCTTACAACAACTTATTGGATATCGACGCTGCCGTGAACTTGATCGATGAGTTCGACGAGCTGACGTTCGCTATCTT
AAAACATAATAACGCTTGCGGTATCGCTTCCCGCGGTAATGTGGTTGAGGCTTGGAAAGAGGCTTTGGCCGGTGACCCGG
TTTCCGCTTTCGGTGGCATCTTGATCACGAATACTACGATTAACAAGGAAGTGGCCGAGGAAATCAACAAGATTTTCTTC
GAGGTGATTATCGCTCCGGCTTACGACGCTGACGCGCTGGAGGTACTTCAACAGAAGAAGAACCGTATCATCTTGATCCG
TAAGGATTGCAAGACTTGCCCGATGCAGTTCCGTTCTTTATTGAATGGTGTCTTGATGCAGGAGAAGGACTTGAGCATCC
AAGGTAAGGCTGATTTGGAGCCTATGACGGATAAGCAGCCGACAGCCTCGGAGGTTGAGGACCTGTTGTTCGCCAACAAA
ATCGTGAAGAACAGCAAGTCCAACGCGATCACCTTGGTGAAGAACAAGCAGCTTTGCGCCAGCGGTATCGGGCAGACCTC
TCGTGTGGACTCTTTGAGACAAGCCATAGAGAAGGCTAAATCATTCGGCTTCGACTTGAATGGCGCCGTGATGGCTTCCG
ACGCTTTCTTCCCGTTCCCCGATTGCGTTGAGATCGCAGACCAAGCAGGTATCACGGCCGTAATCCAGCCGGGAGGCTCT
ATCAATGATAAGTTGTCTGTCGAGTACTGTAACCAACATGGTTTGGCTATGGTGAAGACAGGTGTTCGTCATTTTAAACA
TTAA

Upstream 100 bases:

>100_bases
TTGGTATGGTTGCCTTGTTTTTATCTGAATAATAAGGCTGGATTGGAAATAAATATCTACTTTTGTGCTCTAAAACATTA
AAGTAAACGAAAATAGAAAG

Downstream 100 bases:

>100_bases
TAATAAGACTAATGGGATTGTTTTCTTTCACACAAGAAGTTGCCATAGATTTGGGTACCGCCAATACCATTATCACGTGT
AATGATAAGATGGTGGTAGA

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MAATKRMKRALVSVFHKDGLDEILKKLHSEGVSFVSTGGTQSFIESLGLPCDAVEDLTSYPSILGGRVKTLHPKVFGGIL
ARRENENDQKQLAEYEIPEIDLVIVDLYPFEETVASGAEEQAIIEKIDIGGISLIRAAAKNFKDVVIVASKAQYQPLMDL
LNEKGAETSIEDRRWFAKEAFAVSSGYDSAIFNYFDGREGSHFRATVDSPMHLRYGENPHQAAKFYGKFNDMFDQIHGKE
ISYNNLLDIDAAVNLIDEFDELTFAILKHNNACGIASRGNVVEAWKEALAGDPVSAFGGILITNTTINKEVAEEINKIFF
EVIIAPAYDADALEVLQQKKNRIILIRKDCKTCPMQFRSLLNGVLMQEKDLSIQGKADLEPMTDKQPTASEVEDLLFANK
IVKNSKSNAITLVKNKQLCASGIGQTSRVDSLRQAIEKAKSFGFDLNGAVMASDAFFPFPDCVEIADQAGITAVIQPGGS
INDKLSVEYCNQHGLAMVKTGVRHFKH

Sequences:

>Translated_507_residues
MAATKRMKRALVSVFHKDGLDEILKKLHSEGVSFVSTGGTQSFIESLGLPCDAVEDLTSYPSILGGRVKTLHPKVFGGIL
ARRENENDQKQLAEYEIPEIDLVIVDLYPFEETVASGAEEQAIIEKIDIGGISLIRAAAKNFKDVVIVASKAQYQPLMDL
LNEKGAETSIEDRRWFAKEAFAVSSGYDSAIFNYFDGREGSHFRATVDSPMHLRYGENPHQAAKFYGKFNDMFDQIHGKE
ISYNNLLDIDAAVNLIDEFDELTFAILKHNNACGIASRGNVVEAWKEALAGDPVSAFGGILITNTTINKEVAEEINKIFF
EVIIAPAYDADALEVLQQKKNRIILIRKDCKTCPMQFRSLLNGVLMQEKDLSIQGKADLEPMTDKQPTASEVEDLLFANK
IVKNSKSNAITLVKNKQLCASGIGQTSRVDSLRQAIEKAKSFGFDLNGAVMASDAFFPFPDCVEIADQAGITAVIQPGGS
INDKLSVEYCNQHGLAMVKTGVRHFKH
>Mature_506_residues
AATKRMKRALVSVFHKDGLDEILKKLHSEGVSFVSTGGTQSFIESLGLPCDAVEDLTSYPSILGGRVKTLHPKVFGGILA
RRENENDQKQLAEYEIPEIDLVIVDLYPFEETVASGAEEQAIIEKIDIGGISLIRAAAKNFKDVVIVASKAQYQPLMDLL
NEKGAETSIEDRRWFAKEAFAVSSGYDSAIFNYFDGREGSHFRATVDSPMHLRYGENPHQAAKFYGKFNDMFDQIHGKEI
SYNNLLDIDAAVNLIDEFDELTFAILKHNNACGIASRGNVVEAWKEALAGDPVSAFGGILITNTTINKEVAEEINKIFFE
VIIAPAYDADALEVLQQKKNRIILIRKDCKTCPMQFRSLLNGVLMQEKDLSIQGKADLEPMTDKQPTASEVEDLLFANKI
VKNSKSNAITLVKNKQLCASGIGQTSRVDSLRQAIEKAKSFGFDLNGAVMASDAFFPFPDCVEIADQAGITAVIQPGGSI
NDKLSVEYCNQHGLAMVKTGVRHFKH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=475, Percent_Identity=31.7894736842105, Blast_Score=197, Evalue=3e-50,
Organism=Escherichia coli, GI1790439, Length=531, Percent_Identity=40.1129943502825, Blast_Score=371, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI71985564, Length=507, Percent_Identity=28.9940828402367, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI71985556, Length=132, Percent_Identity=35.6060606060606, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71985574, Length=350, Percent_Identity=23.1428571428571, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323056, Length=478, Percent_Identity=32.2175732217573, Blast_Score=209, Evalue=6e-55,
Organism=Saccharomyces cerevisiae, GI6323768, Length=475, Percent_Identity=30.7368421052632, Blast_Score=198, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24649832, Length=466, Percent_Identity=30.68669527897, Blast_Score=186, Evalue=2e-47,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 55852; Mature: 55721

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATKRMKRALVSVFHKDGLDEILKKLHSEGVSFVSTGGTQSFIESLGLPCDAVEDLTSY
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEECCCHHHHHHHHCCCHHHHHHHHHH
PSILGGRVKTLHPKVFGGILARRENENDQKQLAEYEIPEIDLVIVDLYPFEETVASGAEE
HHHHCCCHHCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEEEECCCHHHHHCCCHH
QAIIEKIDIGGISLIRAAAKNFKDVVIVASKAQYQPLMDLLNEKGAETSIEDRRWFAKEA
HHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHH
FAVSSGYDSAIFNYFDGREGSHFRATVDSPMHLRYGENPHQAAKFYGKFNDMFDQIHGKE
HHHCCCCCHHHHHHCCCCCCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCC
ISYNNLLDIDAAVNLIDEFDELTFAILKHNNACGIASRGNVVEAWKEALAGDPVSAFGGI
CCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHCCE
LITNTTINKEVAEEINKIFFEVIIAPAYDADALEVLQQKKNRIILIRKDCKTCPMQFRSL
EEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHH
LNGVLMQEKDLSIQGKADLEPMTDKQPTASEVEDLLFANKIVKNSKSNAITLVKNKQLCA
HHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHH
SGIGQTSRVDSLRQAIEKAKSFGFDLNGAVMASDAFFPFPDCVEIADQAGITAVIQPGGS
HCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHCCCEEEECCCCC
INDKLSVEYCNQHGLAMVKTGVRHFKH
CCCCEEHEECCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
AATKRMKRALVSVFHKDGLDEILKKLHSEGVSFVSTGGTQSFIESLGLPCDAVEDLTSY
CHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEECCCHHHHHHHHCCCHHHHHHHHHH
PSILGGRVKTLHPKVFGGILARRENENDQKQLAEYEIPEIDLVIVDLYPFEETVASGAEE
HHHHCCCHHCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEEEECCCHHHHHCCCHH
QAIIEKIDIGGISLIRAAAKNFKDVVIVASKAQYQPLMDLLNEKGAETSIEDRRWFAKEA
HHHHHHHCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCCCHHHHHHHHHH
FAVSSGYDSAIFNYFDGREGSHFRATVDSPMHLRYGENPHQAAKFYGKFNDMFDQIHGKE
HHHCCCCCHHHHHHCCCCCCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCC
ISYNNLLDIDAAVNLIDEFDELTFAILKHNNACGIASRGNVVEAWKEALAGDPVSAFGGI
CCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHCCE
LITNTTINKEVAEEINKIFFEVIIAPAYDADALEVLQQKKNRIILIRKDCKTCPMQFRSL
EEECCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHH
LNGVLMQEKDLSIQGKADLEPMTDKQPTASEVEDLLFANKIVKNSKSNAITLVKNKQLCA
HHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHH
SGIGQTSRVDSLRQAIEKAKSFGFDLNGAVMASDAFFPFPDCVEIADQAGITAVIQPGGS
HCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHCCCEEEECCCCC
INDKLSVEYCNQHGLAMVKTGVRHFKH
CCCCEEHEECCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]