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The map label for this gene is mrcA [H]

Identifier: 150008436

GI number: 150008436

Start: 2167897

End: 2170242

Strand: Reverse

Name: mrcA [H]

Synonym: BDI_1818

Alternate gene names: 150008436

Gene position: 2170242-2167897 (Counterclockwise)

Preceding gene: 150008437

Following gene: 150008435

Centisome position: 45.11

GC content: 47.91

Gene sequence:

>2346_bases
ATGGCATCAAAGATTACAAAAGGTATCCTTATTTCTTGTTGGATAGTATACCTCGCTATATTAGGGGCGGTGGTAATGGT
GTTTATGGCGATAGCGAATGGTTCAATTGGTTATATGCCTCCGGTGGAACAATTGGAGAACCCGATTGATAAATATGCTT
CCCAAGTTATCTCCTCGGACGGAAAGGCGTTGGGAGCCTATGCGCATAGCAAAGACAATCGTATTTACGTGAATTATGAG
GATCTTTCGCCGGATTTGGTGAAAGCCTTGATCGCTACCGAGGATATCCGGTTCGCCGAACATAGCGGTATCGATGCGCA
GGGTTTGTTTCGTGCTATCGTGAAGCGGGGTATCTTGATGCAAAAAAGCGGTGGTGGCGGTAGTACCATTACCCAGCAAT
TAGCGAAGCAATTATTCTCGCCGAGTGCCGATAATATGATGGAACGTCTTTTCCAGAAACCGATCGAATGGGTGATCGCG
GTTCAATTGGAACGTTATTACACAAAGGAAGAGATTATAAATATGTATCTGAATAAGTTTGACTTCTTGTACAATGCCGT
GGGAATCCAATCCGCGTCTCGTGTGTATTTTGGAAAGACCCCTAAAACCTTGAAGATCGAGGAAGCCGCCACGTTGGTAG
GCATGTGCAAGAACCCTTCTTATTTCAATCCGGTTCGCCATAACAAGCGTACCATCGGCCGTCGTAATACGGTTTTGGAG
CAGATGGAAAAGGCCGGGTACATAACCAAGGCGGAATGTGATTCGTTGAAGGCTTTACCCTTGGTCGTACATTTTACCCG
TATGGACCATAAAGACGGTTTGGCTCCTTACTTCCGTGAATACCTTCGTCTGACAATGACCGCGAAGAAACCGGAGCGTA
AGGATTACGCCTCTTGGCAATCTCAGAAATTCAGTGAGGACTCATTATCTTGGGCGACGAATCCATTATATGGATGGTGT
AACAAAAATAAAAAAGCCGACGGGGAATACTATAATCTTTATACGGATGGCTTGAAAATCTATACGAGTATCGATTCCCG
TATGCAGAAATATGCGGAAGACGCTGTCCGTGAACATATGAGTAAAGATCTTCAACCGGCTTTCTTCCGGGAAAAGAAGG
GACGTAGCTATGCTCCGTTCTCAAGAGACGTAAGTGTCGGTCAAGTAGACACGATGTTGATGCGTGCGATGCACCAGACA
GATCGTTACCGGGCGATGAAAAAGAGTGGAATGGCTGAGGCCGATATGCGCAAGGAGTTTGAGAAGCCTGTCGATATGCG
TGTGTTTAGTTGGGATGGTCCGATCGATACGATCATGTCTCCCTTGGATTCGATTCGTTATCATAAGAGTTTCTTGCGTA
CGGCGTTTATGTCGATGGACCCTCGCACCGGGCAGGTCAAGGCATATGTAGGTGGTATTGACTATAATGACTTCCAATAT
GATATGGTGAATGGCGGTCGCCGTCAGATCGGTTCTACCATGAAACCGTTCCTTTATTCGTTGGCGATGATCGAGGGGAT
CAGCCCTTGCGACCAGATGCTACATGTGCAGCAGCAGTTGATGGACGAGAACGGTCGTTTGTGGGTTCCCCGTAACGCCA
GCGCGAAGCGAATCGGCGAGATGGTTACGATCAAGTGGGGATTACAGAACTCGGATAACTGGGTAACCGCTTATCTGATG
AGCCAGCTCTCACCTTATACGTTTGTTCGTTTGCTTCATTCTTTCGGTTTGAAGAATCATATCGATCCGGTTATCTCGGT
CTGTCTGGGTACTCCGGATGTATCGGTGGGTGAGATGGTCGGAGCCTATACGGTATTTGCCAACAAGGGCATCCGGGTAG
AACCTTTATTCGTGACCCGTATAGAAGATTCATATGGAAATACAATCGCGAACTTTACCCCACAGATGAGTGAGGTGCTG
ACGGAAGATGCTTCCTATAAGATGCTACATATGTTGAAAAGCGTAATCGACGGTGGTACCGGAGGACGTGTACGTTTCCG
CTACGGTATCAAGGCGGAGATGGGTGGTAAGACCGGAACGACCCAGAATAACTCCGACGGTTGGTTCATGGGATTTACGC
CTAGCTTGGTTTCCGGCTGCTGGGTAGGCGGCGAGGATCGTTCCATCCATTTCGATCGCTTGGCGGAAGGGCAGGGAGCG
AGTATGGCGTTACCGATTTACGCCCTTTATATGCAAAAGGTTTATAAAGATAAAACGTTAGGCTACTCGGAAGACGAGAC
TTTCGAGATCCCCGAGAAATACGCGAACCCATGTAGCGAAGGTGGGGAGGAAGATAGCCCCACGACCCCACCGGATACGG
GAGGCATCGATAAGATGTTTGAGTGA

Upstream 100 bases:

>100_bases
CTCAACTTGCATAAGATCGTATTTTCCTCTATTTTTGTGATCTTAAATAGGTCGTTGTGGATAAGATGAGTCTCTTATCC
ATACCGTGAAATATAAAAAG

Downstream 100 bases:

>100_bases
TAATAATCAGACTATTACAGTTTTTTTTAATTCCTCGTAAGGAAAACTTTTGTTTCCCTATAGCAAAACAAAAGTTTCTT
CAGCAGGAAACTTTTGTTTC

Product: bifunctional family GT51 beta-glycosyltransferase/PBP transpeptidase

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 781; Mature: 780

Protein sequence:

>781_residues
MASKITKGILISCWIVYLAILGAVVMVFMAIANGSIGYMPPVEQLENPIDKYASQVISSDGKALGAYAHSKDNRIYVNYE
DLSPDLVKALIATEDIRFAEHSGIDAQGLFRAIVKRGILMQKSGGGGSTITQQLAKQLFSPSADNMMERLFQKPIEWVIA
VQLERYYTKEEIINMYLNKFDFLYNAVGIQSASRVYFGKTPKTLKIEEAATLVGMCKNPSYFNPVRHNKRTIGRRNTVLE
QMEKAGYITKAECDSLKALPLVVHFTRMDHKDGLAPYFREYLRLTMTAKKPERKDYASWQSQKFSEDSLSWATNPLYGWC
NKNKKADGEYYNLYTDGLKIYTSIDSRMQKYAEDAVREHMSKDLQPAFFREKKGRSYAPFSRDVSVGQVDTMLMRAMHQT
DRYRAMKKSGMAEADMRKEFEKPVDMRVFSWDGPIDTIMSPLDSIRYHKSFLRTAFMSMDPRTGQVKAYVGGIDYNDFQY
DMVNGGRRQIGSTMKPFLYSLAMIEGISPCDQMLHVQQQLMDENGRLWVPRNASAKRIGEMVTIKWGLQNSDNWVTAYLM
SQLSPYTFVRLLHSFGLKNHIDPVISVCLGTPDVSVGEMVGAYTVFANKGIRVEPLFVTRIEDSYGNTIANFTPQMSEVL
TEDASYKMLHMLKSVIDGGTGGRVRFRYGIKAEMGGKTGTTQNNSDGWFMGFTPSLVSGCWVGGEDRSIHFDRLAEGQGA
SMALPIYALYMQKVYKDKTLGYSEDETFEIPEKYANPCSEGGEEDSPTTPPDTGGIDKMFE

Sequences:

>Translated_781_residues
MASKITKGILISCWIVYLAILGAVVMVFMAIANGSIGYMPPVEQLENPIDKYASQVISSDGKALGAYAHSKDNRIYVNYE
DLSPDLVKALIATEDIRFAEHSGIDAQGLFRAIVKRGILMQKSGGGGSTITQQLAKQLFSPSADNMMERLFQKPIEWVIA
VQLERYYTKEEIINMYLNKFDFLYNAVGIQSASRVYFGKTPKTLKIEEAATLVGMCKNPSYFNPVRHNKRTIGRRNTVLE
QMEKAGYITKAECDSLKALPLVVHFTRMDHKDGLAPYFREYLRLTMTAKKPERKDYASWQSQKFSEDSLSWATNPLYGWC
NKNKKADGEYYNLYTDGLKIYTSIDSRMQKYAEDAVREHMSKDLQPAFFREKKGRSYAPFSRDVSVGQVDTMLMRAMHQT
DRYRAMKKSGMAEADMRKEFEKPVDMRVFSWDGPIDTIMSPLDSIRYHKSFLRTAFMSMDPRTGQVKAYVGGIDYNDFQY
DMVNGGRRQIGSTMKPFLYSLAMIEGISPCDQMLHVQQQLMDENGRLWVPRNASAKRIGEMVTIKWGLQNSDNWVTAYLM
SQLSPYTFVRLLHSFGLKNHIDPVISVCLGTPDVSVGEMVGAYTVFANKGIRVEPLFVTRIEDSYGNTIANFTPQMSEVL
TEDASYKMLHMLKSVIDGGTGGRVRFRYGIKAEMGGKTGTTQNNSDGWFMGFTPSLVSGCWVGGEDRSIHFDRLAEGQGA
SMALPIYALYMQKVYKDKTLGYSEDETFEIPEKYANPCSEGGEEDSPTTPPDTGGIDKMFE
>Mature_780_residues
ASKITKGILISCWIVYLAILGAVVMVFMAIANGSIGYMPPVEQLENPIDKYASQVISSDGKALGAYAHSKDNRIYVNYED
LSPDLVKALIATEDIRFAEHSGIDAQGLFRAIVKRGILMQKSGGGGSTITQQLAKQLFSPSADNMMERLFQKPIEWVIAV
QLERYYTKEEIINMYLNKFDFLYNAVGIQSASRVYFGKTPKTLKIEEAATLVGMCKNPSYFNPVRHNKRTIGRRNTVLEQ
MEKAGYITKAECDSLKALPLVVHFTRMDHKDGLAPYFREYLRLTMTAKKPERKDYASWQSQKFSEDSLSWATNPLYGWCN
KNKKADGEYYNLYTDGLKIYTSIDSRMQKYAEDAVREHMSKDLQPAFFREKKGRSYAPFSRDVSVGQVDTMLMRAMHQTD
RYRAMKKSGMAEADMRKEFEKPVDMRVFSWDGPIDTIMSPLDSIRYHKSFLRTAFMSMDPRTGQVKAYVGGIDYNDFQYD
MVNGGRRQIGSTMKPFLYSLAMIEGISPCDQMLHVQQQLMDENGRLWVPRNASAKRIGEMVTIKWGLQNSDNWVTAYLMS
QLSPYTFVRLLHSFGLKNHIDPVISVCLGTPDVSVGEMVGAYTVFANKGIRVEPLFVTRIEDSYGNTIANFTPQMSEVLT
EDASYKMLHMLKSVIDGGTGGRVRFRYGIKAEMGGKTGTTQNNSDGWFMGFTPSLVSGCWVGGEDRSIHFDRLAEGQGAS
MALPIYALYMQKVYKDKTLGYSEDETFEIPEKYANPCSEGGEEDSPTTPPDTGGIDKMFE

Specific function: Cell Wall Formation. Synthesis Of Cross-Linked Peptidoglycan From The Lipid Intermediates. The Enzyme Has A Penicillin-Insensitive Transglycosylase N-Terminal Domain (Formation Of Linear Glycan Strands) And A Penicillin-Sensitive Transpeptidase C-Terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=644, Percent_Identity=27.4844720496894, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI1788867, Length=267, Percent_Identity=31.8352059925094, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786343, Length=290, Percent_Identity=30, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1789601, Length=179, Percent_Identity=30.1675977653631, Blast_Score=74, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 88205; Mature: 88074

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASKITKGILISCWIVYLAILGAVVMVFMAIANGSIGYMPPVEQLENPIDKYASQVISSD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCC
GKALGAYAHSKDNRIYVNYEDLSPDLVKALIATEDIRFAEHSGIDAQGLFRAIVKRGILM
CCEEEHHEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEE
QKSGGGGSTITQQLAKQLFSPSADNMMERLFQKPIEWVIAVQLERYYTKEEIINMYLNKF
EECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFLYNAVGIQSASRVYFGKTPKTLKIEEAATLVGMCKNPSYFNPVRHNKRTIGRRNTVLE
HHHHHHHHHCCCCCEEECCCCCEEEHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHH
QMEKAGYITKAECDSLKALPLVVHFTRMDHKDGLAPYFREYLRLTMTAKKPERKDYASWQ
HHHHCCCEEECCCCCCCCCHHEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
SQKFSEDSLSWATNPLYGWCNKNKKADGEYYNLYTDGLKIYTSIDSRMQKYAEDAVREHM
HHCCCCCHHHHCCCCCEECCCCCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHHH
SKDLQPAFFREKKGRSYAPFSRDVSVGQVDTMLMRAMHQTDRYRAMKKSGMAEADMRKEF
HCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
EKPVDMRVFSWDGPIDTIMSPLDSIRYHKSFLRTAFMSMDPRTGQVKAYVGGIDYNDFQY
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEE
DMVNGGRRQIGSTMKPFLYSLAMIEGISPCDQMLHVQQQLMDENGRLWVPRNASAKRIGE
EHHCCCHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCCCHHHHCC
MVTIKWGLQNSDNWVTAYLMSQLSPYTFVRLLHSFGLKNHIDPVISVCLGTPDVSVGEMV
EEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
GAYTVFANKGIRVEPLFVTRIEDSYGNTIANFTPQMSEVLTEDASYKMLHMLKSVIDGGT
HHHHEEECCCCEEEEEEEEEECHHHCCCHHHCCHHHHHHHHCCCHHHHHHHHHHHHCCCC
GGRVRFRYGIKAEMGGKTGTTQNNSDGWFMGFTPSLVSGCWVGGEDRSIHFDRLAEGQGA
CCEEEEEECCEEECCCCCCCCCCCCCCEEEECCHHHHHCCEECCCCCCEEHHHHCCCCCC
SMALPIYALYMQKVYKDKTLGYSEDETFEIPEKYANPCSEGGEEDSPTTPPDTGGIDKMF
CCHHHHHHHHHHHHHHHCCCCCCCCCCEECCHHHCCCHHCCCCCCCCCCCCCCCCCHHHC
E
C
>Mature Secondary Structure 
ASKITKGILISCWIVYLAILGAVVMVFMAIANGSIGYMPPVEQLENPIDKYASQVISSD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCC
GKALGAYAHSKDNRIYVNYEDLSPDLVKALIATEDIRFAEHSGIDAQGLFRAIVKRGILM
CCEEEHHEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEE
QKSGGGGSTITQQLAKQLFSPSADNMMERLFQKPIEWVIAVQLERYYTKEEIINMYLNKF
EECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFLYNAVGIQSASRVYFGKTPKTLKIEEAATLVGMCKNPSYFNPVRHNKRTIGRRNTVLE
HHHHHHHHHCCCCCEEECCCCCEEEHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHHHH
QMEKAGYITKAECDSLKALPLVVHFTRMDHKDGLAPYFREYLRLTMTAKKPERKDYASWQ
HHHHCCCEEECCCCCCCCCHHEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
SQKFSEDSLSWATNPLYGWCNKNKKADGEYYNLYTDGLKIYTSIDSRMQKYAEDAVREHM
HHCCCCCHHHHCCCCCEECCCCCCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHHHHHH
SKDLQPAFFREKKGRSYAPFSRDVSVGQVDTMLMRAMHQTDRYRAMKKSGMAEADMRKEF
HCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
EKPVDMRVFSWDGPIDTIMSPLDSIRYHKSFLRTAFMSMDPRTGQVKAYVGGIDYNDFQY
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEE
DMVNGGRRQIGSTMKPFLYSLAMIEGISPCDQMLHVQQQLMDENGRLWVPRNASAKRIGE
EHHCCCHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEECCCCCHHHHCC
MVTIKWGLQNSDNWVTAYLMSQLSPYTFVRLLHSFGLKNHIDPVISVCLGTPDVSVGEMV
EEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
GAYTVFANKGIRVEPLFVTRIEDSYGNTIANFTPQMSEVLTEDASYKMLHMLKSVIDGGT
HHHHEEECCCCEEEEEEEEEECHHHCCCHHHCCHHHHHHHHCCCHHHHHHHHHHHHCCCC
GGRVRFRYGIKAEMGGKTGTTQNNSDGWFMGFTPSLVSGCWVGGEDRSIHFDRLAEGQGA
CCEEEEEECCEEECCCCCCCCCCCCCCEEEECCHHHHHCCEECCCCCCEEHHHHCCCCCC
SMALPIYALYMQKVYKDKTLGYSEDETFEIPEKYANPCSEGGEEDSPTTPPDTGGIDKMF
CCHHHHHHHHHHHHHHHCCCCCCCCCCEECCHHHCCCHHCCCCCCCCCCCCCCCCCHHHC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]