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The map label for this gene is hypB [H]

Identifier: 150008423

GI number: 150008423

Start: 2153787

End: 2154539

Strand: Reverse

Name: hypB [H]

Synonym: BDI_1805

Alternate gene names: 150008423

Gene position: 2154539-2153787 (Counterclockwise)

Preceding gene: 150008424

Following gene: 150008422

Centisome position: 44.78

GC content: 47.81

Gene sequence:

>753_bases
ATGTGCGGAACTTGCGGATGCGGAGAGCATCATCACCATCACGATCATATGCATGAGCATTCTCATGGCCATGAACATCA
TCACCATCCTGATGAAGGGATCGTAATCAATCTGGAACAAGATATACTTCAACGTAACAACTTATTGGCGGAGCGAAACC
GGGGATATTTCGAGGCTAAGAACCTGTTCTGCCTGAACTTGATGAGTTCGCCCGGATCCGGTAAGACAACCTTGCTGGAA
GAGACGATCCGCCGTTTGAAAGCGAAGCGATCGATCTATGTGATAGAAGGCGATCAACAAACCTCAAACGATGCCGACCG
TATAGCGGCGTTGGAGATTCCCGTGTTTCAGGTAAATACCGGTACCGGCTGTCATTTGGAAGCCGACATGGTCAATCATG
CCGTTAAGCATCTGAATCCGAAGGAGGGAGCGATCCTTTTTATAGAGAATGTCGGCAATCTGGTCTGTCCGGCCATGTTC
GATCTCGGAGAGTCCAAGAAAGTCGTTATCGTGAGTACGACGGAGGGAGATGATAAACCGTTGAAATACCCACATATCTT
TCAGGAGGCAGATATCTGCGTAATCAACAAGATTGATCTGGCTCCTTATCTGAATACAAAAGTGGAAGTGCTTCGGGAAA
ACGCCTTGAAGGTAAATCACCGTCTCCAGATATTCGAGGTATCCGCCACGAAAGGAGACGGGATGGATGCATGGTGTGAC
TGGTTGCAGACAGAGAGTGCAAAGTGTAAATAA

Upstream 100 bases:

>100_bases
GCTCCTATTTAGTTAAAATCCTTAAAGGGAAGGAACTTCGGGTGAAGTCGATTGTTATTAAAAAGGAATGATATATTAAT
GAAATAAAAACTTAAATACT

Downstream 100 bases:

>100_bases
ATACCCCGGGTTTTAATGAATAAATGGAGAAAAAAACTATATTTGCTCCCATTCATTGAATCTAACTAATCAAAATGTTA
TGATTAACCGAGAATTGATT

Product: hydrogenase accessory protein HypB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MCGTCGCGEHHHHHDHMHEHSHGHEHHHHPDEGIVINLEQDILQRNNLLAERNRGYFEAKNLFCLNLMSSPGSGKTTLLE
ETIRRLKAKRSIYVIEGDQQTSNDADRIAALEIPVFQVNTGTGCHLEADMVNHAVKHLNPKEGAILFIENVGNLVCPAMF
DLGESKKVVIVSTTEGDDKPLKYPHIFQEADICVINKIDLAPYLNTKVEVLRENALKVNHRLQIFEVSATKGDGMDAWCD
WLQTESAKCK

Sequences:

>Translated_250_residues
MCGTCGCGEHHHHHDHMHEHSHGHEHHHHPDEGIVINLEQDILQRNNLLAERNRGYFEAKNLFCLNLMSSPGSGKTTLLE
ETIRRLKAKRSIYVIEGDQQTSNDADRIAALEIPVFQVNTGTGCHLEADMVNHAVKHLNPKEGAILFIENVGNLVCPAMF
DLGESKKVVIVSTTEGDDKPLKYPHIFQEADICVINKIDLAPYLNTKVEVLRENALKVNHRLQIFEVSATKGDGMDAWCD
WLQTESAKCK
>Mature_250_residues
MCGTCGCGEHHHHHDHMHEHSHGHEHHHHPDEGIVINLEQDILQRNNLLAERNRGYFEAKNLFCLNLMSSPGSGKTTLLE
ETIRRLKAKRSIYVIEGDQQTSNDADRIAALEIPVFQVNTGTGCHLEADMVNHAVKHLNPKEGAILFIENVGNLVCPAMF
DLGESKKVVIVSTTEGDDKPLKYPHIFQEADICVINKIDLAPYLNTKVEVLRENALKVNHRLQIFEVSATKGDGMDAWCD
WLQTESAKCK

Specific function: Required for the maturation of the three [NiFe]hydrogenases. Exhibits a low intrinsic GTPase activity. The GTP hydrolysis catalyzed by HypB is an integral process in the incorporation of nickel into hydrogenases [H]

COG id: COG0378

COG function: function code OK; Ni2+-binding GTPase involved in regulation of expression and maturation of urease and hydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypB/hupM family [H]

Homologues:

Organism=Escherichia coli, GI1789082, Length=238, Percent_Identity=52.5210084033613, Blast_Score=249, Evalue=1e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003495
- InterPro:   IPR004392
- InterPro:   IPR012202 [H]

Pfam domain/function: PF02492 cobW [H]

EC number: NA

Molecular weight: Translated: 28107; Mature: 28107

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGTCGCGEHHHHHDHMHEHSHGHEHHHHPDEGIVINLEQDILQRNNLLAERNRGYFEAK
CCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHCCHHHHCCCCEEEEC
NLFCLNLMSSPGSGKTTLLEETIRRLKAKRSIYVIEGDQQTSNDADRIAALEIPVFQVNT
CEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEEEEEEECC
GTGCHLEADMVNHAVKHLNPKEGAILFIENVGNLVCPAMFDLGESKKVVIVSTTEGDDKP
CCCCEEEHHHHHHHHHHCCCCCCEEEEEECCCCEECHHHHCCCCCCEEEEEEECCCCCCC
LKYPHIFQEADICVINKIDLAPYLNTKVEVLRENALKVNHRLQIFEVSATKGDGMDAWCD
CCCCCHHCCCCEEEEEEECCCCCCCHHHHHHHHHHEEECCEEEEEEEECCCCCCHHHHHH
WLQTESAKCK
HHHCCCCCCC
>Mature Secondary Structure
MCGTCGCGEHHHHHDHMHEHSHGHEHHHHPDEGIVINLEQDILQRNNLLAERNRGYFEAK
CCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCEEEEEHHHHHHHCCHHHHCCCCEEEEC
NLFCLNLMSSPGSGKTTLLEETIRRLKAKRSIYVIEGDQQTSNDADRIAALEIPVFQVNT
CEEEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEEEEEEEEECC
GTGCHLEADMVNHAVKHLNPKEGAILFIENVGNLVCPAMFDLGESKKVVIVSTTEGDDKP
CCCCEEEHHHHHHHHHHCCCCCCEEEEEECCCCEECHHHHCCCCCCEEEEEEECCCCCCC
LKYPHIFQEADICVINKIDLAPYLNTKVEVLRENALKVNHRLQIFEVSATKGDGMDAWCD
CCCCCHHCCCCEEEEEEECCCCCCCHHHHHHHHHHEEECCEEEEEEEECCCCCCHHHHHH
WLQTESAKCK
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]