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The map label for this gene is ilvE [H]

Identifier: 150008399

GI number: 150008399

Start: 2112775

End: 2113794

Strand: Reverse

Name: ilvE [H]

Synonym: BDI_1775

Alternate gene names: 150008399

Gene position: 2113794-2112775 (Counterclockwise)

Preceding gene: 150008400

Following gene: 150008398

Centisome position: 43.93

GC content: 49.9

Gene sequence:

>1020_bases
ATGGAAAATTTAAATTGGTCTGAGCTGGGATTTGGTTATATCAAGACAGACTACAATGTAAGATGCTACTATCGTGACGG
CAAATGGGGTGAGTTGGAAGTTTCTTCATCCGAGATTATCAATATTCACATGGCCGCTACTTGCTTGCATTACGGACAAG
AGTCTTTCGAGGGCTTGAAGGCCTTCCGCGGGAAAGACGGTAAGATCCGTGTATTCCGTATGGATGAGAACGCTAAACGT
ATGCAATCTTCCAGCCGGGGTATCAAGATGGCTGAGTTGCCTGTAGAGATGTTCGAGGAAGCTGTACGTAAGGTCGTGAA
ATTGAACGAGCGTTTCGTTCCTCCTTATGAGAGCGGTGCCGCTTTATATATTCGTCCGCTGATGATCGGCTTGGGCGCTC
AGGTGGGTGTGAAGCCGGCTCCCGAATATATGTTTATGATATTTGTCACTCCGGTAGGTCCGTATTTCAAGGGTGGATTC
CAGCCCTCGAAGGTATGCGTACTTCGTGAGTACGACCGTGCGGCTCCTAACGGGACAGGTACGATCAAGGTCGGTGGTAA
TTATGCCGCTAGCTTGGTAGCCGGCGAGATCGCTCATTCCAAGGGATACGCCGCCGTATTGTATCTGGATCCGAAAGAGA
AGAAATATCTGGATGAGTGCGGTCCGGCTAACTTCTTCGGAATCCGTGGCAATAGCTATATCACTCCGCAATCCCACTCG
ATCTTGCCTTCGATCACGAACAAGAGCTTGCAGCAATTGGCCGAGGATATGGGCTTGACGGTAGAGCGTCGTCCGGTTCC
TTTCGAGGAGATCGCTACATTCGACGAGGCTGCCGAGTGTGGTACCGCCGCGGTTATCGCCCCGATCTCGCAAATCGACG
ATTTGGACGAGAACAAATCTTTTGTTATCGCCAAAGATGGCAAACCGGGTCCTGTTTGCGAGAAACTATATCATAAGCTA
CGTGCCATCCAGTATGGCGACGAGCCGGATACTTACGGATGGATCTCGATCATTGAATAA

Upstream 100 bases:

>100_bases
AATCTCCCATAGCGACGGCTATGGGCGTTTTTGCTTTTGAGCGATATTATTAACAGTGTAATTTGTTAATTTTAAACTAA
TTACAGCAAATTGTATAGAT

Downstream 100 bases:

>100_bases
TATCTTTTGTTTCGTAAGGACTGGAGTGCGCTCCGTCTTTACGAGACAGAATACGTTGATAGGCAATTGTTAGTTTGAAC
TTTACTTTTTGCAATAAAAT

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MENLNWSELGFGYIKTDYNVRCYYRDGKWGELEVSSSEIINIHMAATCLHYGQESFEGLKAFRGKDGKIRVFRMDENAKR
MQSSSRGIKMAELPVEMFEEAVRKVVKLNERFVPPYESGAALYIRPLMIGLGAQVGVKPAPEYMFMIFVTPVGPYFKGGF
QPSKVCVLREYDRAAPNGTGTIKVGGNYAASLVAGEIAHSKGYAAVLYLDPKEKKYLDECGPANFFGIRGNSYITPQSHS
ILPSITNKSLQQLAEDMGLTVERRPVPFEEIATFDEAAECGTAAVIAPISQIDDLDENKSFVIAKDGKPGPVCEKLYHKL
RAIQYGDEPDTYGWISIIE

Sequences:

>Translated_339_residues
MENLNWSELGFGYIKTDYNVRCYYRDGKWGELEVSSSEIINIHMAATCLHYGQESFEGLKAFRGKDGKIRVFRMDENAKR
MQSSSRGIKMAELPVEMFEEAVRKVVKLNERFVPPYESGAALYIRPLMIGLGAQVGVKPAPEYMFMIFVTPVGPYFKGGF
QPSKVCVLREYDRAAPNGTGTIKVGGNYAASLVAGEIAHSKGYAAVLYLDPKEKKYLDECGPANFFGIRGNSYITPQSHS
ILPSITNKSLQQLAEDMGLTVERRPVPFEEIATFDEAAECGTAAVIAPISQIDDLDENKSFVIAKDGKPGPVCEKLYHKL
RAIQYGDEPDTYGWISIIE
>Mature_339_residues
MENLNWSELGFGYIKTDYNVRCYYRDGKWGELEVSSSEIINIHMAATCLHYGQESFEGLKAFRGKDGKIRVFRMDENAKR
MQSSSRGIKMAELPVEMFEEAVRKVVKLNERFVPPYESGAALYIRPLMIGLGAQVGVKPAPEYMFMIFVTPVGPYFKGGF
QPSKVCVLREYDRAAPNGTGTIKVGGNYAASLVAGEIAHSKGYAAVLYLDPKEKKYLDECGPANFFGIRGNSYITPQSHS
ILPSITNKSLQQLAEDMGLTVERRPVPFEEIATFDEAAECGTAAVIAPISQIDDLDENKSFVIAKDGKPGPVCEKLYHKL
RAIQYGDEPDTYGWISIIE

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI38176287, Length=341, Percent_Identity=32.258064516129, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI296010906, Length=341, Percent_Identity=32.258064516129, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI296010904, Length=341, Percent_Identity=32.258064516129, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI50658084, Length=332, Percent_Identity=28.3132530120482, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI258614015, Length=297, Percent_Identity=28.6195286195286, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI296010902, Length=337, Percent_Identity=27.5964391691395, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI296010900, Length=341, Percent_Identity=27.2727272727273, Blast_Score=109, Evalue=3e-24,
Organism=Escherichia coli, GI48994963, Length=316, Percent_Identity=28.4810126582279, Blast_Score=122, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17568601, Length=342, Percent_Identity=32.1637426900585, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17565728, Length=356, Percent_Identity=28.0898876404494, Blast_Score=115, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6322608, Length=340, Percent_Identity=30.8823529411765, Blast_Score=142, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6322002, Length=338, Percent_Identity=30.4733727810651, Blast_Score=136, Evalue=6e-33,
Organism=Drosophila melanogaster, GI24641779, Length=354, Percent_Identity=32.7683615819209, Blast_Score=158, Evalue=5e-39,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 37720; Mature: 37720

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLNWSELGFGYIKTDYNVRCYYRDGKWGELEVSSSEIINIHMAATCLHYGQESFEGLK
CCCCCCHHCCCEEEEECCEEEEEEECCCCCEEEECCCCEEEEHHHHHHHHHCHHHHHHHH
AFRGKDGKIRVFRMDENAKRMQSSSRGIKMAELPVEMFEEAVRKVVKLNERFVPPYESGA
HHCCCCCCEEEEEECCHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCC
ALYIRPLMIGLGAQVGVKPAPEYMFMIFVTPVGPYFKGGFQPSKVCVLREYDRAAPNGTG
EEEEEEEHHHCCCCCCCCCCCCEEEEEEEECCCHHHCCCCCCCEEEEEEECCCCCCCCCE
TIKVGGNYAASLVAGEIAHSKGYAAVLYLDPKEKKYLDECGPANFFGIRGNSYITPQSHS
EEEECCCEEEHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCEEEECCCCEECCCCCC
ILPSITNKSLQQLAEDMGLTVERRPVPFEEIATFDEAAECGTAAVIAPISQIDDLDENKS
CCCHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHCCCEEEEHHHHHHCCCCCCCC
FVIAKDGKPGPVCEKLYHKLRAIQYGDEPDTYGWISIIE
EEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MENLNWSELGFGYIKTDYNVRCYYRDGKWGELEVSSSEIINIHMAATCLHYGQESFEGLK
CCCCCCHHCCCEEEEECCEEEEEEECCCCCEEEECCCCEEEEHHHHHHHHHCHHHHHHHH
AFRGKDGKIRVFRMDENAKRMQSSSRGIKMAELPVEMFEEAVRKVVKLNERFVPPYESGA
HHCCCCCCEEEEEECCHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCC
ALYIRPLMIGLGAQVGVKPAPEYMFMIFVTPVGPYFKGGFQPSKVCVLREYDRAAPNGTG
EEEEEEEHHHCCCCCCCCCCCCEEEEEEEECCCHHHCCCCCCCEEEEEEECCCCCCCCCE
TIKVGGNYAASLVAGEIAHSKGYAAVLYLDPKEKKYLDECGPANFFGIRGNSYITPQSHS
EEEECCCEEEHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCEEEECCCCEECCCCCC
ILPSITNKSLQQLAEDMGLTVERRPVPFEEIATFDEAAECGTAAVIAPISQIDDLDENKS
CCCHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHCCCEEEEHHHHHHCCCCCCCC
FVIAKDGKPGPVCEKLYHKLRAIQYGDEPDTYGWISIIE
EEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]