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The map label for this gene is lacA [C]

Identifier: 150008064

GI number: 150008064

Start: 1659085

End: 1659648

Strand: Direct

Name: lacA [C]

Synonym: BDI_1427

Alternate gene names: 150008064

Gene position: 1659085-1659648 (Clockwise)

Preceding gene: 150008063

Following gene: 150008066

Centisome position: 34.48

GC content: 49.65

Gene sequence:

>564_bases
ATGACACTAAACGAATTCTTGAAGTATGTAGAGACAAGAAAGCCTCTTAAAGGAGACGAGATCCATCAGTTTATGAACAA
GATGAGCGATGAGGCCAGACGCATGACATTCGAGTTAAACGGCTCATATCACGATCCCGAGGAAATCCGTGAGCTGTTAT
CCCGCTTGTTTAATAAGCCGGTCGATCCGTCTTTTCGTGTATTCCCCCCGTTCTATACGGATTTTGGGAAGAATATCACG
GTAGGCAAGAACGTTTTCATCAACGCTTGCTGTCATTTTCAAGATCATGGCGGGGTGACACTGGACGACGGTTGCCAGAT
CGGGCATAACGTGGTCTTTGCCACCCTCAACCACGGCTTCGCCCCCGAAGACCGGAGCACTACCTACCCTGCCCCGATCG
TTCTGAAAAAGAATGTCTGGGTAGGCTCGAACGCAACGATCCTATCCGGCGTAACCATTGGCGAGAACGCTATAGTCGGA
GCCGGCTCGGTAGTTACTAAAGATGTACCGGACAATGCCATCGTGGGAGGTGTTCCCGCCAAGGTCATAAAATACATCCA
ATAA

Upstream 100 bases:

>100_bases
AATCCATCTACAAAAGTTTCTCCGGATTTCATCGATCTTTGCCGTTGCAATCGAAAAGGGATAAACAAACCAAGAAGTAG
AACTTATTAAAATAGCAATT

Downstream 100 bases:

>100_bases
CCCGTAACACCGATTACGACATCTGGGATTTCCGATATTCATTCGGGGTACACCCCACGGTCTTCTTGAATATGCGGCTG
AAATGCTGGGAGTATTGGAA

Product: acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MTLNEFLKYVETRKPLKGDEIHQFMNKMSDEARRMTFELNGSYHDPEEIRELLSRLFNKPVDPSFRVFPPFYTDFGKNIT
VGKNVFINACCHFQDHGGVTLDDGCQIGHNVVFATLNHGFAPEDRSTTYPAPIVLKKNVWVGSNATILSGVTIGENAIVG
AGSVVTKDVPDNAIVGGVPAKVIKYIQ

Sequences:

>Translated_187_residues
MTLNEFLKYVETRKPLKGDEIHQFMNKMSDEARRMTFELNGSYHDPEEIRELLSRLFNKPVDPSFRVFPPFYTDFGKNIT
VGKNVFINACCHFQDHGGVTLDDGCQIGHNVVFATLNHGFAPEDRSTTYPAPIVLKKNVWVGSNATILSGVTIGENAIVG
AGSVVTKDVPDNAIVGGVPAKVIKYIQ
>Mature_186_residues
TLNEFLKYVETRKPLKGDEIHQFMNKMSDEARRMTFELNGSYHDPEEIRELLSRLFNKPVDPSFRVFPPFYTDFGKNITV
GKNVFINACCHFQDHGGVTLDDGCQIGHNVVFATLNHGFAPEDRSTTYPAPIVLKKNVWVGSNATILSGVTIGENAIVGA
GSVVTKDVPDNAIVGGVPAKVIKYIQ

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=159, Percent_Identity=37.1069182389937, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1786664, Length=159, Percent_Identity=36.4779874213836, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1788345, Length=122, Percent_Identity=33.6065573770492, Blast_Score=68, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322243, Length=140, Percent_Identity=37.8571428571429, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 20716; Mature: 20585

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLNEFLKYVETRKPLKGDEIHQFMNKMSDEARRMTFELNGSYHDPEEIRELLSRLFNKP
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHCCC
VDPSFRVFPPFYTDFGKNITVGKNVFINACCHFQDHGGVTLDDGCQIGHNVVFATLNHGF
CCCCCEECCCHHHHCCCCEEECCCEEEEEEEEEECCCCEEECCCCCCCCEEEEEEECCCC
APEDRSTTYPAPIVLKKNVWVGSNATILSGVTIGENAIVGAGSVVTKDVPDNAIVGGVPA
CCCCCCCCCCCCEEEECCEEECCCCEEEECEEECCCCEECCCCEEECCCCCCCEECCCHH
KVIKYIQ
HHHHHCC
>Mature Secondary Structure 
TLNEFLKYVETRKPLKGDEIHQFMNKMSDEARRMTFELNGSYHDPEEIRELLSRLFNKP
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHCCC
VDPSFRVFPPFYTDFGKNITVGKNVFINACCHFQDHGGVTLDDGCQIGHNVVFATLNHGF
CCCCCEECCCHHHHCCCCEEECCCEEEEEEEEEECCCCEEECCCCCCCCEEEEEEECCCC
APEDRSTTYPAPIVLKKNVWVGSNATILSGVTIGENAIVGAGSVVTKDVPDNAIVGGVPA
CCCCCCCCCCCCEEEECCEEECCCCEEEECEEECCCCEECCCCEEECCCCCCCEECCCHH
KVIKYIQ
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA