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The map label for this gene is wcaJ [H]

Identifier: 150007993

GI number: 150007993

Start: 1574679

End: 1575323

Strand: Reverse

Name: wcaJ [H]

Synonym: BDI_1355

Alternate gene names: 150007993

Gene position: 1575323-1574679 (Counterclockwise)

Preceding gene: 150007994

Following gene: 150007992

Centisome position: 32.74

GC content: 51.94

Gene sequence:

>645_bases
ATGCTTATAAAAGAGAGTGTTGATAAATCTACTGATTTTATCCCGGATGGGATGACCGCCTTCGAGCGTAACGTGAAGCG
TATAGGGGATTGTCTTTTGGCCTTTATCGCCTTGGTCGTATTCTCCCCCTTGTTCTTGATCTGCTACATCGCCGTGAGGC
GCGAGGATGGCGGCCCTGCCATTTTCAGGCAGGAGCGGATTGGCCGTTTCGGCCATCCGTTTCATATCTATAAGTTCCGC
AGCATGCGTTTGGACGCAGAGAAGTTCGGCCCCGCCCTTTACAAGGGAGGCGCTGATCCCCGTATGACGCATGTGGGCAA
GTTCCTGCGCGAGCATCATTTGGACGAGCTTCCCCAATTATGGAATGTATTCATCGGTGATATGGCCTTCATCGGTCCCC
GTCCGGAACGGAAGTTCTACATCGACCAGATCATGGAACGTGATCCCCGCTATCGTTTCTTGTACCAGATCCGTCCAGGG
GTGACCTCTTACGCCACGCTCTACAACGGCTATACGGATACGCTGGAGAAGATGCTCCGCCGCTTACGCTATGACTTGTT
CTATCTGGAGCACCGCTCGTGGTGGTTCGATTTCAAGATATTGGTGAAGACGTTCTTGAACATTGCGTTCGGGAAGAAAT
TCTAG

Upstream 100 bases:

>100_bases
ACGAGGAACTTTTCCGCCGTCTGGCACGTGAGGCCTTGGAGCGGTATATCGATTACAAGAAAGAGGAGGAGAAAAATAGA
GGAAAGGAATAAATTAAGCC

Downstream 100 bases:

>100_bases
ATAAGGATAACCGAGGAGAGCAATTCTGCCCTCCTTTAAATCAAGAATACCATGAAGAAAGTATTGAAAGGCTGGCTTAT
CGACAATTCGGTGACCACTG

Product: putative capsular polysaccharide biosynthesis glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MLIKESVDKSTDFIPDGMTAFERNVKRIGDCLLAFIALVVFSPLFLICYIAVRREDGGPAIFRQERIGRFGHPFHIYKFR
SMRLDAEKFGPALYKGGADPRMTHVGKFLREHHLDELPQLWNVFIGDMAFIGPRPERKFYIDQIMERDPRYRFLYQIRPG
VTSYATLYNGYTDTLEKMLRRLRYDLFYLEHRSWWFDFKILVKTFLNIAFGKKF

Sequences:

>Translated_214_residues
MLIKESVDKSTDFIPDGMTAFERNVKRIGDCLLAFIALVVFSPLFLICYIAVRREDGGPAIFRQERIGRFGHPFHIYKFR
SMRLDAEKFGPALYKGGADPRMTHVGKFLREHHLDELPQLWNVFIGDMAFIGPRPERKFYIDQIMERDPRYRFLYQIRPG
VTSYATLYNGYTDTLEKMLRRLRYDLFYLEHRSWWFDFKILVKTFLNIAFGKKF
>Mature_214_residues
MLIKESVDKSTDFIPDGMTAFERNVKRIGDCLLAFIALVVFSPLFLICYIAVRREDGGPAIFRQERIGRFGHPFHIYKFR
SMRLDAEKFGPALYKGGADPRMTHVGKFLREHHLDELPQLWNVFIGDMAFIGPRPERKFYIDQIMERDPRYRFLYQIRPG
VTSYATLYNGYTDTLEKMLRRLRYDLFYLEHRSWWFDFKILVKTFLNIAFGKKF

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=189, Percent_Identity=39.6825396825397, Blast_Score=142, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 25473; Mature: 25473

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIKESVDKSTDFIPDGMTAFERNVKRIGDCLLAFIALVVFSPLFLICYIAVRREDGGPA
CCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
IFRQERIGRFGHPFHIYKFRSMRLDAEKFGPALYKGGADPRMTHVGKFLREHHLDELPQL
HHHHHHHCCCCCCEEEEEHHHHCCCHHHHCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
WNVFIGDMAFIGPRPERKFYIDQIMERDPRYRFLYQIRPGVTSYATLYNGYTDTLEKMLR
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHCCHHHHHHHHHH
RLRYDLFYLEHRSWWFDFKILVKTFLNIAFGKKF
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLIKESVDKSTDFIPDGMTAFERNVKRIGDCLLAFIALVVFSPLFLICYIAVRREDGGPA
CCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
IFRQERIGRFGHPFHIYKFRSMRLDAEKFGPALYKGGADPRMTHVGKFLREHHLDELPQL
HHHHHHHCCCCCCEEEEEHHHHCCCHHHHCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
WNVFIGDMAFIGPRPERKFYIDQIMERDPRYRFLYQIRPGVTSYATLYNGYTDTLEKMLR
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHCCHHHHHHHHHH
RLRYDLFYLEHRSWWFDFKILVKTFLNIAFGKKF
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]