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The map label for this gene is ispD2 [H]

Identifier: 150007989

GI number: 150007989

Start: 1570204

End: 1570893

Strand: Reverse

Name: ispD2 [H]

Synonym: BDI_1351

Alternate gene names: 150007989

Gene position: 1570893-1570204 (Counterclockwise)

Preceding gene: 150007990

Following gene: 150007988

Centisome position: 32.65

GC content: 33.48

Gene sequence:

>690_bases
ATGACTAGAAATGTTGCGATAATATTATCCGGTGGATCTGGATCTCGTATGGAGTGTGATATACCTAAACAATATTTGAA
GATACATGGAAAAATGATAATATCTTATAGTTTAGAATGTTTTGTGTGTAATAAGCACATAGATGGCCTTATTATCGCTA
TAGATCCGAAATGGAAACGAATTCTGGAAGATGAAACTAATAGTTTTGATATCCCTATTCATTTTGCTATTTCAGGAGAA
AGTCGGCAATATACGATATTCAATGCGTTAAAGGTGGCAGAAATGTTTTATGCCCCATCTGATATTGTTATTATTCATGA
TGCAGCACGCCCACTTGTAACAGATGTACTTATTGATGAATGTATCAATGTATGCCAAAAATATGATGGGGCTATGCCTG
TTTTACCAATGAAGGATACGATATATGAAAGTTATGATGGACTGAGCATATCTTCTGTCCTTAATAGAGATATATTATTT
GCAGGACAAGCTCCTGAAGCTTTTAAATTAGGGAAATATATAAATGCTTGTAAGTCTATGAATAGGGAAGAATTGTTACT
GGTGAGAGGAAGTGCAGAAATAGCTTATAAGGCAGGAATGAAAGTAAAGTTAATAAAAGGTGATGAACGAAATTTTAAAG
TAACGGTACCGGAGGATTTAGTTCGTTTAAAATTATTGTTGGAAAAATGA

Upstream 100 bases:

>100_bases
AGTGGATGGTCGGTGGAAAATGATTATTAGATTATTTTTTAATAATGTTGCGTTTTATTTTATTAGATAGTGTTGTTCTT
GTATTTATTATAAAGTGATT

Downstream 100 bases:

>100_bases
AAGCAAATGTGTTATTTGGTATAGGTGATTTAAAGTATACGAATATACCACTGCCTAGATTAAAATCAGATGAAGTATTA
GTTAAAGTTAAGGCGGCTGG

Product: nucleotidyltransferase family protein

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase 2; MEP cytidylyltransferase 2; MCT 2 [H]

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTRNVAIILSGGSGSRMECDIPKQYLKIHGKMIISYSLECFVCNKHIDGLIIAIDPKWKRILEDETNSFDIPIHFAISGE
SRQYTIFNALKVAEMFYAPSDIVIIHDAARPLVTDVLIDECINVCQKYDGAMPVLPMKDTIYESYDGLSISSVLNRDILF
AGQAPEAFKLGKYINACKSMNREELLLVRGSAEIAYKAGMKVKLIKGDERNFKVTVPEDLVRLKLLLEK

Sequences:

>Translated_229_residues
MTRNVAIILSGGSGSRMECDIPKQYLKIHGKMIISYSLECFVCNKHIDGLIIAIDPKWKRILEDETNSFDIPIHFAISGE
SRQYTIFNALKVAEMFYAPSDIVIIHDAARPLVTDVLIDECINVCQKYDGAMPVLPMKDTIYESYDGLSISSVLNRDILF
AGQAPEAFKLGKYINACKSMNREELLLVRGSAEIAYKAGMKVKLIKGDERNFKVTVPEDLVRLKLLLEK
>Mature_228_residues
TRNVAIILSGGSGSRMECDIPKQYLKIHGKMIISYSLECFVCNKHIDGLIIAIDPKWKRILEDETNSFDIPIHFAISGES
RQYTIFNALKVAEMFYAPSDIVIIHDAARPLVTDVLIDECINVCQKYDGAMPVLPMKDTIYESYDGLSISSVLNRDILFA
GQAPEAFKLGKYINACKSMNREELLLVRGSAEIAYKAGMKVKLIKGDERNFKVTVPEDLVRLKLLLEK

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=223, Percent_Identity=26.9058295964126, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1789104, Length=230, Percent_Identity=24.7826086956522, Blast_Score=78, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 25752; Mature: 25621

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRNVAIILSGGSGSRMECDIPKQYLKIHGKMIISYSLECFVCNKHIDGLIIAIDPKWKR
CCCEEEEEEECCCCCEEEECCCHHHHHHHCEEEEEEEEEEEEECCCCCEEEEEECCHHHH
ILEDETNSFDIPIHFAISGESRQYTIFNALKVAEMFYAPSDIVIIHDAARPLVTDVLIDE
HHCCCCCCEEEEEEEEECCCCCEEEHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHH
CINVCQKYDGAMPVLPMKDTIYESYDGLSISSVLNRDILFAGQAPEAFKLGKYINACKSM
HHHHHHHCCCCCCCCCCHHHHHHCCCCCCHHHHHCCCEEEECCCCCHHHHHHHHHHHHCC
NREELLLVRGSAEIAYKAGMKVKLIKGDERNFKVTVPEDLVRLKLLLEK
CCCCEEEEECCCEEEEECCCEEEEEECCCCCEEEECHHHHHHHHHHHCC
>Mature Secondary Structure 
TRNVAIILSGGSGSRMECDIPKQYLKIHGKMIISYSLECFVCNKHIDGLIIAIDPKWKR
CCEEEEEEECCCCCEEEECCCHHHHHHHCEEEEEEEEEEEEECCCCCEEEEEECCHHHH
ILEDETNSFDIPIHFAISGESRQYTIFNALKVAEMFYAPSDIVIIHDAARPLVTDVLIDE
HHCCCCCCEEEEEEEEECCCCCEEEHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHH
CINVCQKYDGAMPVLPMKDTIYESYDGLSISSVLNRDILFAGQAPEAFKLGKYINACKSM
HHHHHHHCCCCCCCCCCHHHHHHCCCCCCHHHHHCCCEEEECCCCCHHHHHHHHHHHHCC
NREELLLVRGSAEIAYKAGMKVKLIKGDERNFKVTVPEDLVRLKLLLEK
CCCCEEEEECCCEEEEECCCEEEEEECCCCCEEEECHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]