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The map label for this gene is exoA [H]

Identifier: 150007753

GI number: 150007753

Start: 1285929

End: 1286783

Strand: Direct

Name: exoA [H]

Synonym: BDI_1111

Alternate gene names: 150007753

Gene position: 1285929-1286783 (Clockwise)

Preceding gene: 150007751

Following gene: 150007759

Centisome position: 26.73

GC content: 41.4

Gene sequence:

>855_bases
ATGTACCGATTATGGAATGAAAACAATGTAAAATCGTCAAAAGTTGTATCTTTGAGACAGAATAAAAGTCATTCACCTCT
AAACTTAGAAAAATTGAAAATAATAACGTACAATACAAATGGATTAAGGGCTGCCGTAGGTAAAGGCTTGCCCGAATGGT
TGGCTCAAGAACAGCCGGACGTGTTATGTATCCAAGAGACAAAGTTGCAACCAGACCAGTTCCCGTCAGAAACATTCGAG
ACTTTAGGATATAAGTCTTATTTGTTTAGTGCCCAAAAGAAGGGATATAGTGGCGTGGCCATTCTTACGAAACAGGAACC
GGATCATATCGAGTATGGAATGGGGATCGAGAAGTATGATAATGAGGGACGTTTCCTCCGTGCTGATTTCGGGGATGTAT
CGATCGTAAGTGTCTATCATCCTTCCGGGACTAGTGGGGATGAACGGCAGGATTTTAAGATGATATGGCTGGAAGATTTC
CAGAAATATGTCGTTGAGTTACAGAAATCCCGTCCGAACTTAATTCTCTGTGGCGATTACAACATTTGCCACGAGCCGAT
AGATATTCATGATCCGATCAGGAACGCAAAGAACAGTGGTTTCCTTCCGGAAGAACGGGAATGGATGACTCGCTTCTTAT
CCGCAGGTTTTACGGATACATTTCGCTTCTTGAACCCGGATAAGCAAGAATATACATGGTGGAGTTATCGCTTCAATTCC
AGATCTAAAAATAAAGGATGGCGTATTGATTATTGTATGGTTAGCGACAATATGAAGCCGAAAGTTAAAGCCGCTTATAT
ATTAAACGATGCCGTACATTCTGATCATTGTCCGGCCGTATTAGAGATCGAATAA

Upstream 100 bases:

>100_bases
GGCGGTCGAGGCCGTTATCTGAGTATTCTCTAAAATATCGTTCAAATGTTCCAGCATAGTCTGTCATTCATTCTAAGTTA
TACAAATATAGAAAATTTCT

Downstream 100 bases:

>100_bases
TTTTTCATAATAAAAAGAAAGGATGTGTTCTTTTTGCGTAAGCGAACACATCCTTTTTTATTATTGAAAAAATGGAAATA
AAGTCTATAAATGAACTTAG

Product: exodeoxyribonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MYRLWNENNVKSSKVVSLRQNKSHSPLNLEKLKIITYNTNGLRAAVGKGLPEWLAQEQPDVLCIQETKLQPDQFPSETFE
TLGYKSYLFSAQKKGYSGVAILTKQEPDHIEYGMGIEKYDNEGRFLRADFGDVSIVSVYHPSGTSGDERQDFKMIWLEDF
QKYVVELQKSRPNLILCGDYNICHEPIDIHDPIRNAKNSGFLPEEREWMTRFLSAGFTDTFRFLNPDKQEYTWWSYRFNS
RSKNKGWRIDYCMVSDNMKPKVKAAYILNDAVHSDHCPAVLEIE

Sequences:

>Translated_284_residues
MYRLWNENNVKSSKVVSLRQNKSHSPLNLEKLKIITYNTNGLRAAVGKGLPEWLAQEQPDVLCIQETKLQPDQFPSETFE
TLGYKSYLFSAQKKGYSGVAILTKQEPDHIEYGMGIEKYDNEGRFLRADFGDVSIVSVYHPSGTSGDERQDFKMIWLEDF
QKYVVELQKSRPNLILCGDYNICHEPIDIHDPIRNAKNSGFLPEEREWMTRFLSAGFTDTFRFLNPDKQEYTWWSYRFNS
RSKNKGWRIDYCMVSDNMKPKVKAAYILNDAVHSDHCPAVLEIE
>Mature_284_residues
MYRLWNENNVKSSKVVSLRQNKSHSPLNLEKLKIITYNTNGLRAAVGKGLPEWLAQEQPDVLCIQETKLQPDQFPSETFE
TLGYKSYLFSAQKKGYSGVAILTKQEPDHIEYGMGIEKYDNEGRFLRADFGDVSIVSVYHPSGTSGDERQDFKMIWLEDF
QKYVVELQKSRPNLILCGDYNICHEPIDIHDPIRNAKNSGFLPEEREWMTRFLSAGFTDTFRFLNPDKQEYTWWSYRFNS
RSKNKGWRIDYCMVSDNMKPKVKAAYILNDAVHSDHCPAVLEIE

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=254, Percent_Identity=41.3385826771654, Blast_Score=207, Evalue=9e-54,
Organism=Homo sapiens, GI18375503, Length=254, Percent_Identity=41.3385826771654, Blast_Score=207, Evalue=9e-54,
Organism=Homo sapiens, GI18375501, Length=254, Percent_Identity=41.3385826771654, Blast_Score=207, Evalue=9e-54,
Organism=Homo sapiens, GI18375507, Length=321, Percent_Identity=28.9719626168224, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1788046, Length=238, Percent_Identity=36.5546218487395, Blast_Score=135, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71989536, Length=261, Percent_Identity=34.8659003831418, Blast_Score=164, Evalue=4e-41,
Organism=Drosophila melanogaster, GI221330655, Length=251, Percent_Identity=42.2310756972112, Blast_Score=210, Evalue=7e-55,
Organism=Drosophila melanogaster, GI17136678, Length=251, Percent_Identity=42.2310756972112, Blast_Score=210, Evalue=9e-55,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 33016; Mature: 33016

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRLWNENNVKSSKVVSLRQNKSHSPLNLEKLKIITYNTNGLRAAVGKGLPEWLAQEQPD
CCEECCCCCCCCCEEEEECCCCCCCCCCEEEEEEEEECCCCHHHHHHCCCHHHHHCCCCC
VLCIQETKLQPDQFPSETFETLGYKSYLFSAQKKGYSGVAILTKQEPDHIEYGMGIEKYD
EEEEECCCCCCCCCCHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHCCCCEEEC
NEGRFLRADFGDVSIVSVYHPSGTSGDERQDFKMIWLEDFQKYVVELQKSRPNLILCGDY
CCCCEEEECCCCEEEEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCEEEECCC
NICHEPIDIHDPIRNAKNSGFLPEEREWMTRFLSAGFTDTFRFLNPDKQEYTWWSYRFNS
CCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEEECC
RSKNKGWRIDYCMVSDNMKPKVKAAYILNDAVHSDHCPAVLEIE
CCCCCCEEEEEEEECCCCCCCEEEEEEEECCCCCCCCCEEEEEC
>Mature Secondary Structure
MYRLWNENNVKSSKVVSLRQNKSHSPLNLEKLKIITYNTNGLRAAVGKGLPEWLAQEQPD
CCEECCCCCCCCCEEEEECCCCCCCCCCEEEEEEEEECCCCHHHHHHCCCHHHHHCCCCC
VLCIQETKLQPDQFPSETFETLGYKSYLFSAQKKGYSGVAILTKQEPDHIEYGMGIEKYD
EEEEECCCCCCCCCCHHHHHHCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHCCCCEEEC
NEGRFLRADFGDVSIVSVYHPSGTSGDERQDFKMIWLEDFQKYVVELQKSRPNLILCGDY
CCCCEEEECCCCEEEEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHCCCCEEEECCC
NICHEPIDIHDPIRNAKNSGFLPEEREWMTRFLSAGFTDTFRFLNPDKQEYTWWSYRFNS
CCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEEEECC
RSKNKGWRIDYCMVSDNMKPKVKAAYILNDAVHSDHCPAVLEIE
CCCCCCEEEEEEEECCCCCCCEEEEEEEECCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]