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The map label for this gene is ddh [H]

Identifier: 150007702

GI number: 150007702

Start: 1226546

End: 1227544

Strand: Direct

Name: ddh [H]

Synonym: BDI_1057

Alternate gene names: 150007702

Gene position: 1226546-1227544 (Clockwise)

Preceding gene: 150007700

Following gene: 150007703

Centisome position: 25.49

GC content: 46.05

Gene sequence:

>999_bases
ATGGCGTATAAGATTGCGTTTTTTGGTGCTAAACCCTACGACATCGCTTCTTTCGATAAGATCAATGAGCGGTATGAGTT
CGATATTCGTTACTATAAAGGACATTTGAATATCAATAATGTCGTCTTAACCCAAGGGGTAGACGCTGTATGTATTTTCG
TGAACGACACGGCTGATGCCGCCGTAATAGACGCTTTGGTTGCCAATGGTGTGAAACTGTTGGCGCTTCGTTGCGCCGGT
TTTAATAATGTGGATTTGAATGCCGCCAGGGGGAAATTACCGGTCGTACGCGTACCGGCCTACTCACCTTATGCGGTCGC
TGAATATACGTTGGCCTTGATGTTATCGTTAAACCGTAAGATACACCGGGCATACTGGCGTACGAGAGATGGCAACTTCT
CCTTGAATGGCTTGATGGGATTTGATATGCATGGCAAGACCGCCGGTATTATCGGTACCGGAAAGATCGCCAAGATATTG
ATTCATATCTTAAAAGGATTGGGGATGCGTGTATTGGCAAATGACCTGTATCCGGATGAGAAATTCGCCAAGGAGGAAGG
GATCACCTATGTCTCTTTAGACGAGTTATACAAAGAGTCCGATATCATTTCCTTGCATTGCCCGTTGACTGACCAGACTC
GTTATCTGATCAACGACGATTCGATCGCCAAGATGAAAGACGGCGTAATGATTATCAATACGGGACGCGGGTTACTGATC
CATACCAACGCCTTGATCGAGGGATTGAAGACCAAGAAAGTATCCGCCGCTGGATTGGATGTCTACGAGGAGGAAGGAGA
CTATTTCTACGAGGATAAATCGGATAAGATTATCGATGATGATGTCTTGGCCCGTCTACTCTCGTTCAACAATGTGATCG
TTACTTCCCATCAGGCGTTTTTCACGAAAGAGGCTCTGCATAATATCGCCGATACTACCTTGCGGAACATCAAGGATTTC
GAGGAAGGCCGTCCCTTAGTGAACGAAGTGACCAAGTAA

Upstream 100 bases:

>100_bases
TGTTACCCAGCGGACAAAAGTACTATAATTAAACGAATGGATTTCTTTTTCGATAAACAATCAAGGGCTTAATTTAGTTA
TATTAAAAAACTAAAGTTAT

Downstream 100 bases:

>100_bases
ATTCTAAAAAGGAACTATTATTTATCGCTGATTTATTATATTTTTGTGGCCATTGCTTGGTGGGAACCCAGCAATGGCTT
TTTTAATTCAGAGGTATATA

Product: putative dehydrogenase

Products: pyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MAYKIAFFGAKPYDIASFDKINERYEFDIRYYKGHLNINNVVLTQGVDAVCIFVNDTADAAVIDALVANGVKLLALRCAG
FNNVDLNAARGKLPVVRVPAYSPYAVAEYTLALMLSLNRKIHRAYWRTRDGNFSLNGLMGFDMHGKTAGIIGTGKIAKIL
IHILKGLGMRVLANDLYPDEKFAKEEGITYVSLDELYKESDIISLHCPLTDQTRYLINDDSIAKMKDGVMIINTGRGLLI
HTNALIEGLKTKKVSAAGLDVYEEEGDYFYEDKSDKIIDDDVLARLLSFNNVIVTSHQAFFTKEALHNIADTTLRNIKDF
EEGRPLVNEVTK

Sequences:

>Translated_332_residues
MAYKIAFFGAKPYDIASFDKINERYEFDIRYYKGHLNINNVVLTQGVDAVCIFVNDTADAAVIDALVANGVKLLALRCAG
FNNVDLNAARGKLPVVRVPAYSPYAVAEYTLALMLSLNRKIHRAYWRTRDGNFSLNGLMGFDMHGKTAGIIGTGKIAKIL
IHILKGLGMRVLANDLYPDEKFAKEEGITYVSLDELYKESDIISLHCPLTDQTRYLINDDSIAKMKDGVMIINTGRGLLI
HTNALIEGLKTKKVSAAGLDVYEEEGDYFYEDKSDKIIDDDVLARLLSFNNVIVTSHQAFFTKEALHNIADTTLRNIKDF
EEGRPLVNEVTK
>Mature_331_residues
AYKIAFFGAKPYDIASFDKINERYEFDIRYYKGHLNINNVVLTQGVDAVCIFVNDTADAAVIDALVANGVKLLALRCAGF
NNVDLNAARGKLPVVRVPAYSPYAVAEYTLALMLSLNRKIHRAYWRTRDGNFSLNGLMGFDMHGKTAGIIGTGKIAKILI
HILKGLGMRVLANDLYPDEKFAKEEGITYVSLDELYKESDIISLHCPLTDQTRYLINDDSIAKMKDGVMIINTGRGLLIH
TNALIEGLKTKKVSAAGLDVYEEEGDYFYEDKSDKIIDDDVLARLLSFNNVIVTSHQAFFTKEALHNIADTTLRNIKDFE
EGRPLVNEVTK

Specific function: Fermentative Lactate Dehydrogenase. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=275, Percent_Identity=29.0909090909091, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI61743967, Length=256, Percent_Identity=30.46875, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI4557497, Length=256, Percent_Identity=30.46875, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI145580578, Length=256, Percent_Identity=28.515625, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI4557499, Length=256, Percent_Identity=28.515625, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI145580575, Length=268, Percent_Identity=28.7313432835821, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI6912396, Length=293, Percent_Identity=26.6211604095563, Blast_Score=95, Evalue=9e-20,
Organism=Escherichia coli, GI1787645, Length=328, Percent_Identity=52.1341463414634, Blast_Score=349, Evalue=2e-97,
Organism=Escherichia coli, GI87082289, Length=250, Percent_Identity=31.6, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1789279, Length=243, Percent_Identity=31.6872427983539, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17532191, Length=277, Percent_Identity=31.0469314079422, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI25147481, Length=258, Percent_Identity=29.8449612403101, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6324055, Length=253, Percent_Identity=32.4110671936759, Blast_Score=119, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6320925, Length=237, Percent_Identity=30.8016877637131, Blast_Score=112, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6322116, Length=237, Percent_Identity=30.8016877637131, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324964, Length=219, Percent_Identity=26.027397260274, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19921140, Length=260, Percent_Identity=30.7692307692308, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24646446, Length=265, Percent_Identity=31.6981132075472, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24646448, Length=265, Percent_Identity=31.6981132075472, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24646452, Length=265, Percent_Identity=31.6981132075472, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI24646450, Length=265, Percent_Identity=31.6981132075472, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI62472511, Length=265, Percent_Identity=31.6981132075472, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI28574286, Length=296, Percent_Identity=26.6891891891892, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI28574284, Length=291, Percent_Identity=28.1786941580756, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585514, Length=291, Percent_Identity=28.1786941580756, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28574282, Length=291, Percent_Identity=28.1786941580756, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI45551003, Length=291, Percent_Identity=28.1786941580756, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI45552429, Length=291, Percent_Identity=28.1786941580756, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28571528, Length=299, Percent_Identity=27.7591973244147, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24585516, Length=293, Percent_Identity=24.5733788395904, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.28

Molecular weight: Translated: 37071; Mature: 36940

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYKIAFFGAKPYDIASFDKINERYEFDIRYYKGHLNINNVVLTQGVDAVCIFVNDTADA
CEEEEEEECCCCCCCCCHHHCCCEEEEEEEEEEEEECCCEEEEECCCCEEEEEEECCCCH
AVIDALVANGVKLLALRCAGFNNVDLNAARGKLPVVRVPAYSPYAVAEYTLALMLSLNRK
HHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHH
IHRAYWRTRDGNFSLNGLMGFDMHGKTAGIIGTGKIAKILIHILKGLGMRVLANDLYPDE
HHHHHHCCCCCCEEECCEEEECCCCCEEEEEECCHHHHHHHHHHHCCCCEEHHHHCCCCH
KFAKEEGITYVSLDELYKESDIISLHCPLTDQTRYLINDDSIAKMKDGVMIINTGRGLLI
HHHHHHCCEEEEHHHHHCCCCEEEEECCCCCCCEEEECCCCHHHHHCCEEEEECCCEEEE
HTNALIEGLKTKKVSAAGLDVYEEEGDYFYEDKSDKIIDDDVLARLLSFNNVIVTSHQAF
ECHHHHCCHHHCEEECCCCEEEECCCCEEEECCCCCEECHHHHHHHHHCCCEEEECCHHH
FTKEALHNIADTTLRNIKDFEEGRPLVNEVTK
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCC
>Mature Secondary Structure 
AYKIAFFGAKPYDIASFDKINERYEFDIRYYKGHLNINNVVLTQGVDAVCIFVNDTADA
EEEEEEECCCCCCCCCHHHCCCEEEEEEEEEEEEECCCEEEEECCCCEEEEEEECCCCH
AVIDALVANGVKLLALRCAGFNNVDLNAARGKLPVVRVPAYSPYAVAEYTLALMLSLNRK
HHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHH
IHRAYWRTRDGNFSLNGLMGFDMHGKTAGIIGTGKIAKILIHILKGLGMRVLANDLYPDE
HHHHHHCCCCCCEEECCEEEECCCCCEEEEEECCHHHHHHHHHHHCCCCEEHHHHCCCCH
KFAKEEGITYVSLDELYKESDIISLHCPLTDQTRYLINDDSIAKMKDGVMIINTGRGLLI
HHHHHHCCEEEEHHHHHCCCCEEEEECCCCCCCEEEECCCCHHHHHCCEEEEECCCEEEE
HTNALIEGLKTKKVSAAGLDVYEEEGDYFYEDKSDKIIDDDVLARLLSFNNVIVTSHQAF
ECHHHHCCHHHCEEECCCCEEEECCCCEEEECCCCCEECHHHHHHHHHCCCEEEECCHHH
FTKEALHNIADTTLRNIKDFEEGRPLVNEVTK
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; NAD+

Specific reaction: (R)-lactate + NAD+ = pyruvate + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Pyruvate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]