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The map label for this gene is oprM [H]

Identifier: 150007618

GI number: 150007618

Start: 1126906

End: 1128270

Strand: Reverse

Name: oprM [H]

Synonym: BDI_0971

Alternate gene names: 150007618

Gene position: 1128270-1126906 (Counterclockwise)

Preceding gene: 150007619

Following gene: 150007617

Centisome position: 23.45

GC content: 53.41

Gene sequence:

>1365_bases
ATGAAGAATAACATCATACTCTTTGCCGTAGCGACGCTAGCGTTGAATAGCTGCGGCATTTACAAGAAATACGAGCCTGT
CGCCACCCTCCCGGAGAATCTATATCGGGAGGAGATAGCCCCGGCAGATACCTTCTCCATCGGGAATATCAGTTGGCGGG
ACCTATTCACCGATCCGGCCTTGCAATCCTTGATCGAACGGGGATTAGCCAATAATACAGATCTGCGGATTGCCCATCTA
AAGGTGCAAGAAGCCGAGGCGGCGTTAATGACCTCCCGCCTCTCCTACCTCCCGTCGCTCTCCCTAGATCCGCAGGGCAC
GACAAGTAGTTTCAATAAGGCAAAAGCGTCGTGGACATACAACGTCCCGGTATCGGCCAGTTGGGAGATCGATATCTTCG
GCCGGCTGACAAACGCCAAACGCCAAGCGAAAGCCGCCTTGTCGCAAAGCCAAGCGTATAGCCAAGCCGTACAAACACAA
CTGATCGCCAACATCGCCAATTTATATTATACCCTATTGATGCTGGATCGGCAATACGAGATCACGACGGAAACCGCCGT
GAGATGGCAAGAGAGCCTTCGTGCCACCCAAGCGTTAATGGCAGCCGGCATGACTACCGAGGCGGCCGTATCGCAAACCG
AGGCTACTTGCTACTCCATCGAGACCTCTGTTAAGGATCTGGAACAGACCATCCGGGAGACCGAGAATACGCTTGCCGTC
TTATTAGGAGAAACACCTCAAGATATCGAGCGAGGAAGATTGGAAGAGCAGTCCTTGACCCCGGATCTGGCGATCGGCAT
CCCGGTGCAGCTGCTATCGAACCGTCCGGATGTACGTAGCGCCGAGTACGCATTGGCGCAAGCGTTCTACGGGACAGCCT
CCGCACGCTCCAATTTTTATCCCTCGCTACGTCTTAGCGGCAGCGCAGGGTGGACAAACAGCGCAGGCAGTTACATCGTA
AATCCGGGTAAACTATTACTATCGGCGGTCGGCTCCCTCACGCAACCGCTATTTAACAAAGGAGCGAATATCGCCCAATT
GAAAATAGCGAAAGCGCAGCAAGAGGAGGCCAAGCTCACTTTCCAACAAACCGTGCTCAACGCCGGAAAAGAGGTGAACG
ACGCACTTACCCAAGCCCAGACCGCAAAGGGAAAGATCGACTTGCGTACACACCAAATCGAATCGCTTGAAGATGCCGTG
CGTAGCACCCAATTGCTGATGACGCATGGAAACACTACTTATTTGGAAGTGCTCACGGCACAGCAAACCCTGCTCAGCGC
CCAGCTCTCCCAAGTAACGGATCGTTTTGACGAGCTTCAAGCCACCGTAAACCTCTACCAAGCCTTAGGAGGAGGCAGAC
ATTAA

Upstream 100 bases:

>100_bases
GATTGGCAGGTGCAAGAAGAGGTAAAAGAGGCCGCCGAAGAAAGAAAAGAATATTTAGATAGCAAGAAATAATCTATCCA
GTTGTATATATATAATGTAT

Downstream 100 bases:

>100_bases
AGAGAGTCCTTTCAGACTTGTATATAATTATGATTGTAACCTCCTTTTGTGCGTGACGCATAGAAGGAGGTAAAAAAATA
CCCGGTTTATGGTATATGCC

Product: multidrug efflux pump channel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MKNNIILFAVATLALNSCGIYKKYEPVATLPENLYREEIAPADTFSIGNISWRDLFTDPALQSLIERGLANNTDLRIAHL
KVQEAEAALMTSRLSYLPSLSLDPQGTTSSFNKAKASWTYNVPVSASWEIDIFGRLTNAKRQAKAALSQSQAYSQAVQTQ
LIANIANLYYTLLMLDRQYEITTETAVRWQESLRATQALMAAGMTTEAAVSQTEATCYSIETSVKDLEQTIRETENTLAV
LLGETPQDIERGRLEEQSLTPDLAIGIPVQLLSNRPDVRSAEYALAQAFYGTASARSNFYPSLRLSGSAGWTNSAGSYIV
NPGKLLLSAVGSLTQPLFNKGANIAQLKIAKAQQEEAKLTFQQTVLNAGKEVNDALTQAQTAKGKIDLRTHQIESLEDAV
RSTQLLMTHGNTTYLEVLTAQQTLLSAQLSQVTDRFDELQATVNLYQALGGGRH

Sequences:

>Translated_454_residues
MKNNIILFAVATLALNSCGIYKKYEPVATLPENLYREEIAPADTFSIGNISWRDLFTDPALQSLIERGLANNTDLRIAHL
KVQEAEAALMTSRLSYLPSLSLDPQGTTSSFNKAKASWTYNVPVSASWEIDIFGRLTNAKRQAKAALSQSQAYSQAVQTQ
LIANIANLYYTLLMLDRQYEITTETAVRWQESLRATQALMAAGMTTEAAVSQTEATCYSIETSVKDLEQTIRETENTLAV
LLGETPQDIERGRLEEQSLTPDLAIGIPVQLLSNRPDVRSAEYALAQAFYGTASARSNFYPSLRLSGSAGWTNSAGSYIV
NPGKLLLSAVGSLTQPLFNKGANIAQLKIAKAQQEEAKLTFQQTVLNAGKEVNDALTQAQTAKGKIDLRTHQIESLEDAV
RSTQLLMTHGNTTYLEVLTAQQTLLSAQLSQVTDRFDELQATVNLYQALGGGRH
>Mature_454_residues
MKNNIILFAVATLALNSCGIYKKYEPVATLPENLYREEIAPADTFSIGNISWRDLFTDPALQSLIERGLANNTDLRIAHL
KVQEAEAALMTSRLSYLPSLSLDPQGTTSSFNKAKASWTYNVPVSASWEIDIFGRLTNAKRQAKAALSQSQAYSQAVQTQ
LIANIANLYYTLLMLDRQYEITTETAVRWQESLRATQALMAAGMTTEAAVSQTEATCYSIETSVKDLEQTIRETENTLAV
LLGETPQDIERGRLEEQSLTPDLAIGIPVQLLSNRPDVRSAEYALAQAFYGTASARSNFYPSLRLSGSAGWTNSAGSYIV
NPGKLLLSAVGSLTQPLFNKGANIAQLKIAKAQQEEAKLTFQQTVLNAGKEVNDALTQAQTAKGKIDLRTHQIESLEDAV
RSTQLLMTHGNTTYLEVLTAQQTLLSAQLSQVTDRFDELQATVNLYQALGGGRH

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=458, Percent_Identity=29.6943231441048, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI2367348, Length=469, Percent_Identity=21.7484008528785, Blast_Score=89, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 49725; Mature: 49725

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNNIILFAVATLALNSCGIYKKYEPVATLPENLYREEIAPADTFSIGNISWRDLFTDPA
CCCCEEEEEHHHHHHHCCCCCCCCCCHHHCHHHHHHHHCCCCCCEECCCEEHHHHHCCHH
LQSLIERGLANNTDLRIAHLKVQEAEAALMTSRLSYLPSLSLDPQGTTSSFNKAKASWTY
HHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEE
NVPVSASWEIDIFGRLTNAKRQAKAALSQSQAYSQAVQTQLIANIANLYYTLLMLDRQYE
ECCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
ITTETAVRWQESLRATQALMAAGMTTEAAVSQTEATCYSIETSVKDLEQTIRETENTLAV
EHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LLGETPQDIERGRLEEQSLTPDLAIGIPVQLLSNRPDVRSAEYALAQAFYGTASARSNFY
EECCCHHHHHHCCCHHHHCCCCEEECCCHHHHCCCCCCCHHHHHHHHHHHCCHHCCCCCC
PSLRLSGSAGWTNSAGSYIVNPGKLLLSAVGSLTQPLFNKGANIAQLKIAKAQQEEAKLT
CCEEECCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHH
FQQTVLNAGKEVNDALTQAQTAKGKIDLRTHQIESLEDAVRSTQLLMTHGNTTYLEVLTA
HHHHHHHCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHEEEEECCCCEEEEEHHH
QQTLLSAQLSQVTDRFDELQATVNLYQALGGGRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKNNIILFAVATLALNSCGIYKKYEPVATLPENLYREEIAPADTFSIGNISWRDLFTDPA
CCCCEEEEEHHHHHHHCCCCCCCCCCHHHCHHHHHHHHCCCCCCEECCCEEHHHHHCCHH
LQSLIERGLANNTDLRIAHLKVQEAEAALMTSRLSYLPSLSLDPQGTTSSFNKAKASWTY
HHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEE
NVPVSASWEIDIFGRLTNAKRQAKAALSQSQAYSQAVQTQLIANIANLYYTLLMLDRQYE
ECCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
ITTETAVRWQESLRATQALMAAGMTTEAAVSQTEATCYSIETSVKDLEQTIRETENTLAV
EHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LLGETPQDIERGRLEEQSLTPDLAIGIPVQLLSNRPDVRSAEYALAQAFYGTASARSNFY
EECCCHHHHHHCCCHHHHCCCCEEECCCHHHHCCCCCCCHHHHHHHHHHHCCHHCCCCCC
PSLRLSGSAGWTNSAGSYIVNPGKLLLSAVGSLTQPLFNKGANIAQLKIAKAQQEEAKLT
CCEEECCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHH
FQQTVLNAGKEVNDALTQAQTAKGKIDLRTHQIESLEDAVRSTQLLMTHGNTTYLEVLTA
HHHHHHHCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHEEEEECCCCEEEEEHHH
QQTLLSAQLSQVTDRFDELQATVNLYQALGGGRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]