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The map label for this gene is rimO

Identifier: 150007589

GI number: 150007589

Start: 1092560

End: 1093858

Strand: Direct

Name: rimO

Synonym: BDI_0942

Alternate gene names: 150007589

Gene position: 1092560-1093858 (Clockwise)

Preceding gene: 150007588

Following gene: 150007590

Centisome position: 22.71

GC content: 52.12

Gene sequence:

>1299_bases
ATGAGAAAAAATAAAGTAGATATTATTACGCTGGGGTGTTCGAAAAACCTAGTGGACTCGGAGCAACTTATGCGCCAATT
CGTTGCCAATGGCTATACGGTAGAGCATGATCCCCATAAGATCAATGGCGAGATCGTGGTGGTGAATACCTGCGGTTTCA
TCGGCGACGCCCAAGAGGAATCGATCAATATGATCCTCGAGCTGGGCGAGCAAAAGCAGAAAGGACGGATCGGGAAGCTC
TTCGTGATGGGGTGCTTGTCGGAGCGTTTCTTGAAAGATCTGGAGAAGGAACTTCCCGAGGTAGATCGTTTCTACGGGAA
ATTCAATTGGAAGGAGTTGATCTCCGATCTGGGCAAGTCGTACCATCAAGAGCTGGCTACGGATCGTGTGCTCACCACCC
CGAGGCACTACGCTTACGTGAAGATCGGGGAGGGATGCAACCGTACTTGTTCTTACTGTTCGATCCCGATCATAACTGGG
GCTTATCAATCCCGTCCGATGGACGAGATCGTAGACGAGGTTCGTGGCTTGGTCGCGCAAGGCGTGAAAGAATTCCAGAT
GATCGCCCAAGATCTTACGTTTTATGGCCTCGATCGCTATAAGCGAATGGCCTTGCCGGAACTGGTGGAGCGGGTATCCG
ACATCCCGGGCGTGGAGTGGATTCGTTTGCATTACGGGTATCCGTCTCATTTCCCCTATGACCTGTTGCCGGTGATGCGT
GAGCGTGATAACGTTTGCAAGTATATGGACATCGCCCTCCAGCATATCAGCGACCCGATGTTGAAGATGATGCGCCGCAA
TATCACGAAGGCCGAGACCTATGAGCTGTTGGAGCGTATGCGCCGGGAAGTGCCGGGCATCCATCTACGCACCACCTTGA
TGGTGGGTCATCCCGGCGAGACGGAACAGGATTTCGAGGAGTTGATCCGCTTCGTGAAAGATATCCGTTTCGAGCGCATG
GGCGCTTTCGCCTATAGCCACGAGGAGGGTACCTACGCCTATCAGCATTATAAAGACGAGATTCCACAGGAGGTGAAGCA
AGACCGGCTGGATTACTTGATGCGTGTGCAAGAAGGCATCTCCGCCGACGTGAACGCCTCGAAAGTAGGGCAGACCTTTC
GGGTGATCGTAGACCGGGAGGAGGAGGATTTCTACGTGGGACGTACGCAATACGACTCGCCCGAGGTAGATCCGGAAATA
TTGATCTCCAAGGATACTCCCTTGAGTCCGGGTAGCTTTTACCAAGTAAAGGTGATTGACGCACAAGCGTTTGATCTGTA
TGGTAAAGTGTTGAATTGA

Upstream 100 bases:

>100_bases
ACCATTTCCGTATCCCCGTGAAATATATCGGACTCGGCGAGGGGATGGAGGATTTGCAAGTATTTCGCAAACGTGAGTTT
GTCAATTCGTTATTTGGAGA

Downstream 100 bases:

>100_bases
TGTAAAAACTGTCAATATATTTGTCGGTTAACGAAAAAATAACTAATATAGCAGCACGAACTGTTATGGAATTAGATATG
AAAAACAAGGAACTTATAAC

Product: putative Fe-S oxidoreductase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MRKNKVDIITLGCSKNLVDSEQLMRQFVANGYTVEHDPHKINGEIVVVNTCGFIGDAQEESINMILELGEQKQKGRIGKL
FVMGCLSERFLKDLEKELPEVDRFYGKFNWKELISDLGKSYHQELATDRVLTTPRHYAYVKIGEGCNRTCSYCSIPIITG
AYQSRPMDEIVDEVRGLVAQGVKEFQMIAQDLTFYGLDRYKRMALPELVERVSDIPGVEWIRLHYGYPSHFPYDLLPVMR
ERDNVCKYMDIALQHISDPMLKMMRRNITKAETYELLERMRREVPGIHLRTTLMVGHPGETEQDFEELIRFVKDIRFERM
GAFAYSHEEGTYAYQHYKDEIPQEVKQDRLDYLMRVQEGISADVNASKVGQTFRVIVDREEEDFYVGRTQYDSPEVDPEI
LISKDTPLSPGSFYQVKVIDAQAFDLYGKVLN

Sequences:

>Translated_432_residues
MRKNKVDIITLGCSKNLVDSEQLMRQFVANGYTVEHDPHKINGEIVVVNTCGFIGDAQEESINMILELGEQKQKGRIGKL
FVMGCLSERFLKDLEKELPEVDRFYGKFNWKELISDLGKSYHQELATDRVLTTPRHYAYVKIGEGCNRTCSYCSIPIITG
AYQSRPMDEIVDEVRGLVAQGVKEFQMIAQDLTFYGLDRYKRMALPELVERVSDIPGVEWIRLHYGYPSHFPYDLLPVMR
ERDNVCKYMDIALQHISDPMLKMMRRNITKAETYELLERMRREVPGIHLRTTLMVGHPGETEQDFEELIRFVKDIRFERM
GAFAYSHEEGTYAYQHYKDEIPQEVKQDRLDYLMRVQEGISADVNASKVGQTFRVIVDREEEDFYVGRTQYDSPEVDPEI
LISKDTPLSPGSFYQVKVIDAQAFDLYGKVLN
>Mature_432_residues
MRKNKVDIITLGCSKNLVDSEQLMRQFVANGYTVEHDPHKINGEIVVVNTCGFIGDAQEESINMILELGEQKQKGRIGKL
FVMGCLSERFLKDLEKELPEVDRFYGKFNWKELISDLGKSYHQELATDRVLTTPRHYAYVKIGEGCNRTCSYCSIPIITG
AYQSRPMDEIVDEVRGLVAQGVKEFQMIAQDLTFYGLDRYKRMALPELVERVSDIPGVEWIRLHYGYPSHFPYDLLPVMR
ERDNVCKYMDIALQHISDPMLKMMRRNITKAETYELLERMRREVPGIHLRTTLMVGHPGETEQDFEELIRFVKDIRFERM
GAFAYSHEEGTYAYQHYKDEIPQEVKQDRLDYLMRVQEGISADVNASKVGQTFRVIVDREEEDFYVGRTQYDSPEVDPEI
LISKDTPLSPGSFYQVKVIDAQAFDLYGKVLN

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=484, Percent_Identity=26.6528925619835, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI28872782, Length=484, Percent_Identity=26.6528925619835, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI93277076, Length=416, Percent_Identity=26.2019230769231, Blast_Score=121, Evalue=2e-27,
Organism=Escherichia coli, GI1787057, Length=443, Percent_Identity=37.020316027088, Blast_Score=277, Evalue=9e-76,
Organism=Escherichia coli, GI1786882, Length=450, Percent_Identity=28, Blast_Score=156, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17553146, Length=467, Percent_Identity=23.982869379015, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI71996771, Length=313, Percent_Identity=26.1980830670927, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21356207, Length=478, Percent_Identity=25.7322175732218, Blast_Score=158, Evalue=6e-39,
Organism=Drosophila melanogaster, GI19922432, Length=459, Percent_Identity=25.4901960784314, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_PARD8 (A6LAJ6)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001302332.1
- STRING:   A6LAJ6
- GeneID:   5306096
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_0942
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   CLSK822448
- BioCyc:   PDIS435591:BDI_0942-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50113; Mature: 50113

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKNKVDIITLGCSKNLVDSEQLMRQFVANGYTVEHDPHKINGEIVVVNTCGFIGDAQEE
CCCCCEEEEEECCCCCCCCHHHHHHHHHHCCEEECCCCCEECCEEEEEECCCCCCCCCHH
SINMILELGEQKQKGRIGKLFVMGCLSERFLKDLEKELPEVDRFYGKFNWKELISDLGKS
HHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHH
YHQELATDRVLTTPRHYAYVKIGEGCNRTCSYCSIPIITGAYQSRPMDEIVDEVRGLVAQ
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHH
GVKEFQMIAQDLTFYGLDRYKRMALPELVERVSDIPGVEWIRLHYGYPSHFPYDLLPVMR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHH
ERDNVCKYMDIALQHISDPMLKMMRRNITKAETYELLERMRREVPGIHLRTTLMVGHPGE
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCC
TEQDFEELIRFVKDIRFERMGAFAYSHEEGTYAYQHYKDEIPQEVKQDRLDYLMRVQEGI
CHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SADVNASKVGQTFRVIVDREEEDFYVGRTQYDSPEVDPEILISKDTPLSPGSFYQVKVID
CCCCCHHHHCCEEEEEEECCCCCEEECCCCCCCCCCCCCEEEECCCCCCCCCEEEEEEEC
AQAFDLYGKVLN
CHHHHHHHHHCC
>Mature Secondary Structure
MRKNKVDIITLGCSKNLVDSEQLMRQFVANGYTVEHDPHKINGEIVVVNTCGFIGDAQEE
CCCCCEEEEEECCCCCCCCHHHHHHHHHHCCEEECCCCCEECCEEEEEECCCCCCCCCHH
SINMILELGEQKQKGRIGKLFVMGCLSERFLKDLEKELPEVDRFYGKFNWKELISDLGKS
HHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHH
YHQELATDRVLTTPRHYAYVKIGEGCNRTCSYCSIPIITGAYQSRPMDEIVDEVRGLVAQ
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHH
GVKEFQMIAQDLTFYGLDRYKRMALPELVERVSDIPGVEWIRLHYGYPSHFPYDLLPVMR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHH
ERDNVCKYMDIALQHISDPMLKMMRRNITKAETYELLERMRREVPGIHLRTTLMVGHPGE
HHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCC
TEQDFEELIRFVKDIRFERMGAFAYSHEEGTYAYQHYKDEIPQEVKQDRLDYLMRVQEGI
CHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SADVNASKVGQTFRVIVDREEEDFYVGRTQYDSPEVDPEILISKDTPLSPGSFYQVKVID
CCCCCHHHHCCEEEEEEECCCCCEEECCCCCCCCCCCCCEEEECCCCCCCCCEEEEEEEC
AQAFDLYGKVLN
CHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA