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The map label for this gene is pheS

Identifier: 150007485

GI number: 150007485

Start: 984122

End: 985141

Strand: Reverse

Name: pheS

Synonym: BDI_0836

Alternate gene names: 150007485

Gene position: 985141-984122 (Counterclockwise)

Preceding gene: 150007487

Following gene: 150007484

Centisome position: 20.48

GC content: 45.1

Gene sequence:

>1020_bases
ATGCTCGATAAAATTAAGGCTCTGCTTCAAGAAGTAGAGAATATGAAAGCGGCAAATGCCGAGGAACTAGAAGCTTTACG
TATAAAGTATCTCAGCAAAAAAGGCGAGATATCGGCGTTGATGAATGATTTCCGTAATGTAGCGGCTGATCAAAAACGTG
AGGTTGGCAAATACTTGAACGAACTAAAGGAAACAACCCAGAGCAAGATCAACGAATTAAAAGAAAGCTTTGAGAATGTA
CAGACCGCAAACGATGATATCGATTTGACTCGTACGGCTTACCCGATCGAACTAGGCACTCGTCATCCTCTATCACTTGT
AAAGAAAGAAATTTGCGATATATTCGGACGGTTAGGATTCAGCATCGCTGAGGGACCGGAAATCGAGGACGACTGGCATG
TCTTCTCTTCCTTGAATTTCGCCGAGGACCATCCTGCCCGCGATATGCAAGATACGTTCTTTATCCAGCATAACCCGGAT
GTATTGCTTCGCACACATACTTCTTCCGTACAGACCCGAGTAATGGAAAAGCAAGAACCGCCTATTCGTATCATCTGTCC
GGGACGTGTATATCGTAATGAGGCTATCTCCTATCGTGCTCACTGTTTCTTCCATCAAGTAGAAGCGTTATATGTAGATA
AGAACGTCTCATTCGCCGACTTGAAACAAGCCTTGTTGTTCTTCGCCAAGGAAATGTTCAGTCCGGATACAAAAATTCGC
TTGCGTCCTTCTTACTTCCCGTTCACGGAGCCATCCGCCGAGATGGACATCTCCTGCAACATTTGTGGAGGTAAGGGTTG
CCCCTTCTGTAAAGGAACCGGTTGGGTAGAGATCCTCGGTTGCGGTATGGTAGACCCGAACGTGCTTGAGAACTGCGGTA
TCGATAGCAAGGTTTATTCAGGCTACGCATTGGGAATGGGTATCGAGCGTATTACCAATTTGAAATACCAAGTAAAAGAC
TTACGCATGTTCTCGGAAAACGATGTTCGTTTCTTGAGACAGTTTGAGTCGGCCAATTAA

Upstream 100 bases:

>100_bases
AAGTGTCTGCGCTTACATTTAACTAAAAAACCTTACGAATCCCTTGCGGTTTCAAAAGAAAGGCGTACTTTTGCATGACA
AATACAAAGTGTGATTAGAA

Downstream 100 bases:

>100_bases
ATTGAAAACCTAAATCATTTACCATCTCCCCGGGCCTTAACCTAGCCCGGGGGAAACACACTAGGTTGATTATGATAAAG
GTTTTTCTTTTAATAATAGG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MLDKIKALLQEVENMKAANAEELEALRIKYLSKKGEISALMNDFRNVAADQKREVGKYLNELKETTQSKINELKESFENV
QTANDDIDLTRTAYPIELGTRHPLSLVKKEICDIFGRLGFSIAEGPEIEDDWHVFSSLNFAEDHPARDMQDTFFIQHNPD
VLLRTHTSSVQTRVMEKQEPPIRIICPGRVYRNEAISYRAHCFFHQVEALYVDKNVSFADLKQALLFFAKEMFSPDTKIR
LRPSYFPFTEPSAEMDISCNICGGKGCPFCKGTGWVEILGCGMVDPNVLENCGIDSKVYSGYALGMGIERITNLKYQVKD
LRMFSENDVRFLRQFESAN

Sequences:

>Translated_339_residues
MLDKIKALLQEVENMKAANAEELEALRIKYLSKKGEISALMNDFRNVAADQKREVGKYLNELKETTQSKINELKESFENV
QTANDDIDLTRTAYPIELGTRHPLSLVKKEICDIFGRLGFSIAEGPEIEDDWHVFSSLNFAEDHPARDMQDTFFIQHNPD
VLLRTHTSSVQTRVMEKQEPPIRIICPGRVYRNEAISYRAHCFFHQVEALYVDKNVSFADLKQALLFFAKEMFSPDTKIR
LRPSYFPFTEPSAEMDISCNICGGKGCPFCKGTGWVEILGCGMVDPNVLENCGIDSKVYSGYALGMGIERITNLKYQVKD
LRMFSENDVRFLRQFESAN
>Mature_339_residues
MLDKIKALLQEVENMKAANAEELEALRIKYLSKKGEISALMNDFRNVAADQKREVGKYLNELKETTQSKINELKESFENV
QTANDDIDLTRTAYPIELGTRHPLSLVKKEICDIFGRLGFSIAEGPEIEDDWHVFSSLNFAEDHPARDMQDTFFIQHNPD
VLLRTHTSSVQTRVMEKQEPPIRIICPGRVYRNEAISYRAHCFFHQVEALYVDKNVSFADLKQALLFFAKEMFSPDTKIR
LRPSYFPFTEPSAEMDISCNICGGKGCPFCKGTGWVEILGCGMVDPNVLENCGIDSKVYSGYALGMGIERITNLKYQVKD
LRMFSENDVRFLRQFESAN

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=295, Percent_Identity=29.4915254237288, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI4758340, Length=285, Percent_Identity=28.0701754385965, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1788007, Length=325, Percent_Identity=46.7692307692308, Blast_Score=296, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI32566635, Length=287, Percent_Identity=29.616724738676, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI32563657, Length=268, Percent_Identity=31.3432835820896, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17508957, Length=268, Percent_Identity=31.3432835820896, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321087, Length=269, Percent_Identity=30.1115241635688, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6325304, Length=283, Percent_Identity=25.0883392226148, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17137424, Length=297, Percent_Identity=26.2626262626263, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI18858079, Length=275, Percent_Identity=28, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_PARD8 (A6LA92)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001302228.1
- ProteinModelPortal:   A6LA92
- SMR:   A6LA92
- STRING:   A6LA92
- GeneID:   5305990
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_0836
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- BioCyc:   PDIS435591:BDI_0836-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 38726; Mature: 38726

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDKIKALLQEVENMKAANAEELEALRIKYLSKKGEISALMNDFRNVAADQKREVGKYLN
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ELKETTQSKINELKESFENVQTANDDIDLTRTAYPIELGTRHPLSLVKKEICDIFGRLGF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCC
SIAEGPEIEDDWHVFSSLNFAEDHPARDMQDTFFIQHNPDVLLRTHTSSVQTRVMEKQEP
CCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEEECCHHHHHHHHHCCCC
PIRIICPGRVYRNEAISYRAHCFFHQVEALYVDKNVSFADLKQALLFFAKEMFSPDTKIR
CEEEEECCHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEE
LRPSYFPFTEPSAEMDISCNICGGKGCPFCKGTGWVEILGCGMVDPNVLENCGIDSKVYS
ECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHCCCCCHHHC
GYALGMGIERITNLKYQVKDLRMFSENDVRFLRQFESAN
CCHHHCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure
MLDKIKALLQEVENMKAANAEELEALRIKYLSKKGEISALMNDFRNVAADQKREVGKYLN
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ELKETTQSKINELKESFENVQTANDDIDLTRTAYPIELGTRHPLSLVKKEICDIFGRLGF
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCC
SIAEGPEIEDDWHVFSSLNFAEDHPARDMQDTFFIQHNPDVLLRTHTSSVQTRVMEKQEP
CCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCEEEEEECCCEEEEECCHHHHHHHHHCCCC
PIRIICPGRVYRNEAISYRAHCFFHQVEALYVDKNVSFADLKQALLFFAKEMFSPDTKIR
CEEEEECCHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEE
LRPSYFPFTEPSAEMDISCNICGGKGCPFCKGTGWVEILGCGMVDPNVLENCGIDSKVYS
ECCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCHHHHHHCCCCCHHHC
GYALGMGIERITNLKYQVKDLRMFSENDVRFLRQFESAN
CCHHHCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA