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The map label for this gene is tgt [H]

Identifier: 150007423

GI number: 150007423

Start: 905737

End: 906867

Strand: Reverse

Name: tgt [H]

Synonym: BDI_0774

Alternate gene names: 150007423

Gene position: 906867-905737 (Counterclockwise)

Preceding gene: 150007424

Following gene: 150007422

Centisome position: 18.85

GC content: 50.84

Gene sequence:

>1131_bases
ATGACATTTGAATTACAGTATAACGATACGAAATCGAACGCTAGGGCGGGCTTGATTACCACCGATCACGGGGTAATCGA
GACGCCTATTTTTATGCCCGTGGGTACGCAAGGTACGGTAAAGGGTGTACATATGACCGAGCTAAAGGAAGATACCAAGG
CACAAATCATTTTGGGGAATACGTACCATCTGTATCTTCGTCCCGGTATCCAGATCTTGGAGCAGGCCGGAGGCTTGCAT
AAGTTTAATACATGGGATCGCCCGATATTGACCGATAGCGGGGGGTTTCAGGTATTCTCTCTTTCCGACAATCGTAAGCT
GCATGAAGAGGGAGCCGAGTTCCGCTCGCATCTCGACGGCTCCAAGCATATGTTCACGCCGGAGAAGGTGATGGATATCG
AACGCTCTATCGGTGCGGATATCATCATGGCCTTCGATGAGTGTTGCCCGGGAGATGCCGACTACAATTACGCCAAGCAA
TCACTGGGCCTGACCGAGCGTTGGCTGGATCGTTGTTTCGCCCGCTTCAACAGCACGGAGCCGAAATACGGCTACCGGCA
AAGTCTCTTCCCGATCGTACAGGGATGCGTCTATACGGACTTGCGGCAGAGGGCGGCGGAGAACGTGGCCCGAAAAGGCG
CCGATGGAAACGCTATCGGGGGACTGGCCGTTGGCGAGCCTACCGATAAGATGTATGAGATGATCGAGGTCGTAAACGAT
ATCTTACCGAAAGATAAACCTCGTTACTTGATGGGCGTAGGTACCCCGATCAATTTACTGGAAGGGATCGAACGAGGCAT
CGATATGTTCGACTGTATCATGCCAACCCGCAACGGGCGCAACGGCCAACTTTTCACGCGTTTCGGTACTATCAATATGC
GTAACAAGAAATGGGAGAACGATTTCTCGCCGGTCGATCCGGACGGACATTCCTACGTGGATACCTTATATAGCAAGGCT
TATCTGCACCACTTGATCAAGGCGAAAGAAATGCTTGGCTTACAGATCGCTTCTATCCATAACCTCGCCTTCTACCTATG
GTTGGTGAAAGAAGCGAGGGCACATATCATCGCTGGCGATTTTACTACATGGAAAAGTGGTATGGTCCGTCAATTAGCGA
ACCGGTTATAA

Upstream 100 bases:

>100_bases
GTTCGTGGAGAAGCCTAAAGAGGAAGAGGTGAAAAAGGTAGAAATAGATGACTTCGGATTCTAAGAGAAAAATGCTACCT
TTGTCACAAATTTTTGATAC

Downstream 100 bases:

>100_bases
AAAACGAGACAGATGAAATTTAAACTAAAACGGATCGATTGGTATATCATCAAGCAGTTTCTGGGCACGTACGTGTTCGC
TATCGCCTTGATTATCTCTA

Product: tRNA-guanine transglycosylase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MTFELQYNDTKSNARAGLITTDHGVIETPIFMPVGTQGTVKGVHMTELKEDTKAQIILGNTYHLYLRPGIQILEQAGGLH
KFNTWDRPILTDSGGFQVFSLSDNRKLHEEGAEFRSHLDGSKHMFTPEKVMDIERSIGADIIMAFDECCPGDADYNYAKQ
SLGLTERWLDRCFARFNSTEPKYGYRQSLFPIVQGCVYTDLRQRAAENVARKGADGNAIGGLAVGEPTDKMYEMIEVVND
ILPKDKPRYLMGVGTPINLLEGIERGIDMFDCIMPTRNGRNGQLFTRFGTINMRNKKWENDFSPVDPDGHSYVDTLYSKA
YLHHLIKAKEMLGLQIASIHNLAFYLWLVKEARAHIIAGDFTTWKSGMVRQLANRL

Sequences:

>Translated_376_residues
MTFELQYNDTKSNARAGLITTDHGVIETPIFMPVGTQGTVKGVHMTELKEDTKAQIILGNTYHLYLRPGIQILEQAGGLH
KFNTWDRPILTDSGGFQVFSLSDNRKLHEEGAEFRSHLDGSKHMFTPEKVMDIERSIGADIIMAFDECCPGDADYNYAKQ
SLGLTERWLDRCFARFNSTEPKYGYRQSLFPIVQGCVYTDLRQRAAENVARKGADGNAIGGLAVGEPTDKMYEMIEVVND
ILPKDKPRYLMGVGTPINLLEGIERGIDMFDCIMPTRNGRNGQLFTRFGTINMRNKKWENDFSPVDPDGHSYVDTLYSKA
YLHHLIKAKEMLGLQIASIHNLAFYLWLVKEARAHIIAGDFTTWKSGMVRQLANRL
>Mature_375_residues
TFELQYNDTKSNARAGLITTDHGVIETPIFMPVGTQGTVKGVHMTELKEDTKAQIILGNTYHLYLRPGIQILEQAGGLHK
FNTWDRPILTDSGGFQVFSLSDNRKLHEEGAEFRSHLDGSKHMFTPEKVMDIERSIGADIIMAFDECCPGDADYNYAKQS
LGLTERWLDRCFARFNSTEPKYGYRQSLFPIVQGCVYTDLRQRAAENVARKGADGNAIGGLAVGEPTDKMYEMIEVVNDI
LPKDKPRYLMGVGTPINLLEGIERGIDMFDCIMPTRNGRNGQLFTRFGTINMRNKKWENDFSPVDPDGHSYVDTLYSKAY
LHHLIKAKEMLGLQIASIHNLAFYLWLVKEARAHIIAGDFTTWKSGMVRQLANRL

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=352, Percent_Identity=40.0568181818182, Blast_Score=283, Evalue=2e-76,
Organism=Escherichia coli, GI1786607, Length=364, Percent_Identity=50.5494505494505, Blast_Score=363, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17544680, Length=344, Percent_Identity=38.6627906976744, Blast_Score=249, Evalue=1e-66,
Organism=Caenorhabditis elegans, GI25146502, Length=143, Percent_Identity=28.6713286713287, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19920510, Length=364, Percent_Identity=42.5824175824176, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI21355311, Length=275, Percent_Identity=25.4545454545455, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42496; Mature: 42365

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFELQYNDTKSNARAGLITTDHGVIETPIFMPVGTQGTVKGVHMTELKEDTKAQIILGN
CEEEEEECCCCCCCCCCEEECCCCCEECCEEEECCCCCCCCCCCHHHHCCCCCEEEEECC
TYHLYLRPGIQILEQAGGLHKFNTWDRPILTDSGGFQVFSLSDNRKLHEEGAEFRSHLDG
EEEEEECCHHHHHHHCCCCCCCCCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHCCC
SKHMFTPEKVMDIERSIGADIIMAFDECCPGDADYNYAKQSLGLTERWLDRCFARFNSTE
CCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCC
PKYGYRQSLFPIVQGCVYTDLRQRAAENVARKGADGNAIGGLAVGEPTDKMYEMIEVVND
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ILPKDKPRYLMGVGTPINLLEGIERGIDMFDCIMPTRNGRNGQLFTRFGTINMRNKKWEN
HCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCCC
DFSPVDPDGHSYVDTLYSKAYLHHLIKAKEMLGLQIASIHNLAFYLWLVKEARAHIIAGD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECC
FTTWKSGMVRQLANRL
CHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TFELQYNDTKSNARAGLITTDHGVIETPIFMPVGTQGTVKGVHMTELKEDTKAQIILGN
EEEEEECCCCCCCCCCEEECCCCCEECCEEEECCCCCCCCCCCHHHHCCCCCEEEEECC
TYHLYLRPGIQILEQAGGLHKFNTWDRPILTDSGGFQVFSLSDNRKLHEEGAEFRSHLDG
EEEEEECCHHHHHHHCCCCCCCCCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHCCC
SKHMFTPEKVMDIERSIGADIIMAFDECCPGDADYNYAKQSLGLTERWLDRCFARFNSTE
CCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCC
PKYGYRQSLFPIVQGCVYTDLRQRAAENVARKGADGNAIGGLAVGEPTDKMYEMIEVVND
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
ILPKDKPRYLMGVGTPINLLEGIERGIDMFDCIMPTRNGRNGQLFTRFGTINMRNKKWEN
HCCCCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCCC
DFSPVDPDGHSYVDTLYSKAYLHHLIKAKEMLGLQIASIHNLAFYLWLVKEARAHIIAGD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECC
FTTWKSGMVRQLANRL
CHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]