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The map label for this gene is ispE

Identifier: 150007364

GI number: 150007364

Start: 847890

End: 848705

Strand: Reverse

Name: ispE

Synonym: BDI_0715

Alternate gene names: 150007364

Gene position: 848705-847890 (Counterclockwise)

Preceding gene: 150007370

Following gene: 150007363

Centisome position: 17.64

GC content: 52.21

Gene sequence:

>816_bases
ATGATCTGTTTCCCGAATGCCAAGATAAACCTAGGTCTGAACGTGGTAAGCAAACGTCCGGACGGTTATCATAATATAGA
GACCATCTTCTATCCGATCCCCGTGAGGGATGCCCTCGAGATCGTCGCCTCGGATCGGACCTGTTTCACGCAAACCGGAA
TCCCGGTAGACGCTCCCCAAGAGAAGAACCTCGTCATAAAAGCACTGAACGCCCTAAAGACGAGATACGAGATTCCCCCG
CTAGAGATCCATTTGTTGAAAGCGATACCTTTTGGCGCCGGGCTTGGAGGAGGTTCCGCCGATGCCGCTTTTATGTTGAA
GCTGGTCAATGATTTCTGCGGTTTGGATATTCACCCGGATGAGTTGGAGGCGATAGCGTCCACCATCGGAGCCGATTGCC
CGTTCTTTATCCGTAACACGCCGGTATTCGCGACCGGCACGGGAAACCAATTCGAGCCCGTGGACTTGTCCTTGAAGGAT
TATTATCTATGCTTGGTAAAACCGGATGTAGCCGTCTCCACCCCCGAAGCCTATTCGATGGTAACACCCGCCGCGCCCGA
GACCTCCTTGAAAGAGATCATCCGGCTACCGGTATCCGAATGGAAAGAGCGGATGGTGAACGATTTCGAGCGAAGCGTAT
TCCCGAGACATCCCGTGATCGAGCGGATCAAGGATACCTTATACGAGGGAGGCGCCTTATATGCCGCCATGTCCGGCTCC
GGATCGTCCGTGTTCGGTTTGTTCGAAAAACCAACCCATTTCAAGGAACAATCCCTGTTCAGCGATTGTTTTCTATGGGA
AGGACAATTATCATGA

Upstream 100 bases:

>100_bases
GCCCCTTATTAAAGCCATATATAGTTCACCCTATAAAACAGAACGTTTATAAGGATATCTTCTTATCTTCGCGTATCACA
TAAAACAAACATTAAATACT

Downstream 100 bases:

>100_bases
ATCAAATAGAGACTTTTTTTGATACGATGGCGGAACGCTGGGACGCTGTCTGCGTACATGATCCGGGAAAGATCCGGACG
ATATTGGATCGTACCAACCT

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MICFPNAKINLGLNVVSKRPDGYHNIETIFYPIPVRDALEIVASDRTCFTQTGIPVDAPQEKNLVIKALNALKTRYEIPP
LEIHLLKAIPFGAGLGGGSADAAFMLKLVNDFCGLDIHPDELEAIASTIGADCPFFIRNTPVFATGTGNQFEPVDLSLKD
YYLCLVKPDVAVSTPEAYSMVTPAAPETSLKEIIRLPVSEWKERMVNDFERSVFPRHPVIERIKDTLYEGGALYAAMSGS
GSSVFGLFEKPTHFKEQSLFSDCFLWEGQLS

Sequences:

>Translated_271_residues
MICFPNAKINLGLNVVSKRPDGYHNIETIFYPIPVRDALEIVASDRTCFTQTGIPVDAPQEKNLVIKALNALKTRYEIPP
LEIHLLKAIPFGAGLGGGSADAAFMLKLVNDFCGLDIHPDELEAIASTIGADCPFFIRNTPVFATGTGNQFEPVDLSLKD
YYLCLVKPDVAVSTPEAYSMVTPAAPETSLKEIIRLPVSEWKERMVNDFERSVFPRHPVIERIKDTLYEGGALYAAMSGS
GSSVFGLFEKPTHFKEQSLFSDCFLWEGQLS
>Mature_271_residues
MICFPNAKINLGLNVVSKRPDGYHNIETIFYPIPVRDALEIVASDRTCFTQTGIPVDAPQEKNLVIKALNALKTRYEIPP
LEIHLLKAIPFGAGLGGGSADAAFMLKLVNDFCGLDIHPDELEAIASTIGADCPFFIRNTPVFATGTGNQFEPVDLSLKD
YYLCLVKPDVAVSTPEAYSMVTPAAPETSLKEIIRLPVSEWKERMVNDFERSVFPRHPVIERIKDTLYEGGALYAAMSGS
GSSVFGLFEKPTHFKEQSLFSDCFLWEGQLS

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=262, Percent_Identity=32.0610687022901, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_PARD8 (A6L9X1)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001302107.1
- ProteinModelPortal:   A6L9X1
- SMR:   A6L9X1
- STRING:   A6L9X1
- GeneID:   5305869
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_0715
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   PAVMVNP
- ProtClustDB:   PRK14609
- BioCyc:   PDIS435591:BDI_0715-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 29879; Mature: 29879

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 132-132

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MICFPNAKINLGLNVVSKRPDGYHNIETIFYPIPVRDALEIVASDRTCFTQTGIPVDAPQ
CEECCCCEEEECEEEEECCCCCCCCCEEEEEECCHHHHHHHHHCCCEEEEECCCCCCCCC
EKNLVIKALNALKTRYEIPPLEIHLLKAIPFGAGLGGGSADAAFMLKLVNDFCGLDIHPD
CCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHH
ELEAIASTIGADCPFFIRNTPVFATGTGNQFEPVDLSLKDYYLCLVKPDVAVSTPEAYSM
HHHHHHHHHCCCCCEEEECCCEEEECCCCCCCCEEEECCCEEEEEECCCCEECCCCHHEE
VTPAAPETSLKEIIRLPVSEWKERMVNDFERSVFPRHPVIERIKDTLYEGGALYAAMSGS
ECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEECCC
GSSVFGLFEKPTHFKEQSLFSDCFLWEGQLS
CCEEEEECCCCCCHHHHHHHHHHEEECCCCC
>Mature Secondary Structure
MICFPNAKINLGLNVVSKRPDGYHNIETIFYPIPVRDALEIVASDRTCFTQTGIPVDAPQ
CEECCCCEEEECEEEEECCCCCCCCCEEEEEECCHHHHHHHHHCCCEEEEECCCCCCCCC
EKNLVIKALNALKTRYEIPPLEIHLLKAIPFGAGLGGGSADAAFMLKLVNDFCGLDIHPD
CCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHH
ELEAIASTIGADCPFFIRNTPVFATGTGNQFEPVDLSLKDYYLCLVKPDVAVSTPEAYSM
HHHHHHHHHCCCCCEEEECCCEEEECCCCCCCCEEEECCCEEEEEECCCCEECCCCHHEE
VTPAAPETSLKEIIRLPVSEWKERMVNDFERSVFPRHPVIERIKDTLYEGGALYAAMSGS
ECCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEECCC
GSSVFGLFEKPTHFKEQSLFSDCFLWEGQLS
CCEEEEECCCCCCHHHHHHHHHHEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA