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The map label for this gene is nrfA [H]

Identifier: 150007328

GI number: 150007328

Start: 803750

End: 805210

Strand: Reverse

Name: nrfA [H]

Synonym: BDI_0675

Alternate gene names: 150007328

Gene position: 805210-803750 (Counterclockwise)

Preceding gene: 150007329

Following gene: 150007326

Centisome position: 16.74

GC content: 47.98

Gene sequence:

>1461_bases
TTGAAAGCACACTATAAAACTTCTATAGCCATAAGTCTAATCGTCATTATCGTATTAGTGATCTTACGTACCACTGTTAC
CGGACAAGATAGCATCAAATTCATCGACGATACCGATGATCAGCCAATCGATCAACAAAACAGCGACTCTATAGACCTTC
GTTATTCCAATGAGTACCAAAGTTGGAAACAAACCTCGGACACGACTTTCCGCAGTTTGTTTAATGGGAACCAACAAGCT
GATATCTTGGCCGAACATCCCGGAATGATGGTATTATGGGCAGGATACGCTTTCTCCAAAGATTATCTAAGTCCCAGAGG
GCATATGTATTCCATAGAAGATCTATACAAAAGCCTGCGGACCGGCGCTCCCATGACCCCGACGAGTGGTCCCCAGCCTG
CCGCATGCTGGGCATGTAAAAGTCCGGACATACCCCGCTTATTAACGACCACCGATGCCAAGACCTTATATAAGAAGAAA
TGGGCGGAGCTAGGCAACGAGATCGTAAATCCAATCGGATGCGCAGACTGCCATGAACCGCAAAGCATGGGATTGCGCGT
GACCCGCTCATTCTTGAAAAGTGCCTATAAGCGCAACGGATTACGTATAGAGGACGCAACCGAGCAAGAGATGCGATCTC
TAGTATGCGCCCAATGCCACGCCGAATATTATTTCCAAGGAGAAGATCGTATACTAACCTTACCTTGGGATCAAGGTTAT
ACGGTAGAAAATATAGAAGCCTATTACGATAGCATAAACTTCACCGATTTCACCCATAAATTAAGCCGGGCACATTTGAT
AAAGGCGCAACATCCGGATTTCGAGTTATTCCAGATGGGCATTCACGGGCAAAGAGGCGTTTCCTGCGGAGAATGCCATA
TGCCTGCTGTCGGGGAAGATGAGAACCGGTACAACAACCATCATATCAAAAGCCCGTTGGCGATGATAGACCGTACTTGC
CAAGCCTGCCACCGGGAAAGTGAGGAGACCTTACGCAAGGATGTTTATGAACGGCAAAACAAAGTCTACGCCATACGCAT
GCGTGTAGAGGAAGAATTGACAAAAGCCCATATAGAAGCGAAATTCGCTTGGGACAAAGGCGCTACGGAATCTCAAATGA
AAAACGTATTAAAACTGCTCCGGCAGGCACAATGGCGTTGGGACTTCGCCGTGGCCTCACACGGGGCGGCTTTCCACGCG
CCCCAAGAAGTCACACGTATCTTGGGCAGCGGACTCGATAAGGCTACCCAAGCCCGCATCCAGATCATGAAGGTCTTAGC
GCAGCTAGGCTATACCCAAGATGTTCCGATGCCGGATATCTCCACAAAAGCGAAAGCCCAGCAATATATCGGCCTCGATA
TGGAAGCCGAGCAACAGGCCAAACAAAAGTTTTTAGAAACCGTCATCCCCCAATGGCAGGAAGAAGCGAGGGCCAATAAA
CGATTCATCGAAGGTAATTAA

Upstream 100 bases:

>100_bases
TGAAATAAGGATTTTTTCAATTTCTACCGGTCGTATCATTATTATTCATATATTCGCAGTTAATACAAAGAATATTTTAT
TTAAGATAAAAGGTAAACAC

Downstream 100 bases:

>100_bases
GTTCTTTCCTCCAGCTCCAATCCCGGGGTATAAGGTAGCGAGATCGTAAACATACTACCTACGCCAACCTCGGATTCCAG
CGATATGCTTCCATGAAACG

Product: cytochrome c552

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MKAHYKTSIAISLIVIIVLVILRTTVTGQDSIKFIDDTDDQPIDQQNSDSIDLRYSNEYQSWKQTSDTTFRSLFNGNQQA
DILAEHPGMMVLWAGYAFSKDYLSPRGHMYSIEDLYKSLRTGAPMTPTSGPQPAACWACKSPDIPRLLTTTDAKTLYKKK
WAELGNEIVNPIGCADCHEPQSMGLRVTRSFLKSAYKRNGLRIEDATEQEMRSLVCAQCHAEYYFQGEDRILTLPWDQGY
TVENIEAYYDSINFTDFTHKLSRAHLIKAQHPDFELFQMGIHGQRGVSCGECHMPAVGEDENRYNNHHIKSPLAMIDRTC
QACHRESEETLRKDVYERQNKVYAIRMRVEEELTKAHIEAKFAWDKGATESQMKNVLKLLRQAQWRWDFAVASHGAAFHA
PQEVTRILGSGLDKATQARIQIMKVLAQLGYTQDVPMPDISTKAKAQQYIGLDMEAEQQAKQKFLETVIPQWQEEARANK
RFIEGN

Sequences:

>Translated_486_residues
MKAHYKTSIAISLIVIIVLVILRTTVTGQDSIKFIDDTDDQPIDQQNSDSIDLRYSNEYQSWKQTSDTTFRSLFNGNQQA
DILAEHPGMMVLWAGYAFSKDYLSPRGHMYSIEDLYKSLRTGAPMTPTSGPQPAACWACKSPDIPRLLTTTDAKTLYKKK
WAELGNEIVNPIGCADCHEPQSMGLRVTRSFLKSAYKRNGLRIEDATEQEMRSLVCAQCHAEYYFQGEDRILTLPWDQGY
TVENIEAYYDSINFTDFTHKLSRAHLIKAQHPDFELFQMGIHGQRGVSCGECHMPAVGEDENRYNNHHIKSPLAMIDRTC
QACHRESEETLRKDVYERQNKVYAIRMRVEEELTKAHIEAKFAWDKGATESQMKNVLKLLRQAQWRWDFAVASHGAAFHA
PQEVTRILGSGLDKATQARIQIMKVLAQLGYTQDVPMPDISTKAKAQQYIGLDMEAEQQAKQKFLETVIPQWQEEARANK
RFIEGN
>Mature_486_residues
MKAHYKTSIAISLIVIIVLVILRTTVTGQDSIKFIDDTDDQPIDQQNSDSIDLRYSNEYQSWKQTSDTTFRSLFNGNQQA
DILAEHPGMMVLWAGYAFSKDYLSPRGHMYSIEDLYKSLRTGAPMTPTSGPQPAACWACKSPDIPRLLTTTDAKTLYKKK
WAELGNEIVNPIGCADCHEPQSMGLRVTRSFLKSAYKRNGLRIEDATEQEMRSLVCAQCHAEYYFQGEDRILTLPWDQGY
TVENIEAYYDSINFTDFTHKLSRAHLIKAQHPDFELFQMGIHGQRGVSCGECHMPAVGEDENRYNNHHIKSPLAMIDRTC
QACHRESEETLRKDVYERQNKVYAIRMRVEEELTKAHIEAKFAWDKGATESQMKNVLKLLRQAQWRWDFAVASHGAAFHA
PQEVTRILGSGLDKATQARIQIMKVLAQLGYTQDVPMPDISTKAKAQQYIGLDMEAEQQAKQKFLETVIPQWQEEARANK
RFIEGN

Specific function: Plays a role in nitrite reduction [H]

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family [H]

Homologues:

Organism=Escherichia coli, GI1790506, Length=434, Percent_Identity=45.3917050691244, Blast_Score=417, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031 [H]

Pfam domain/function: PF02335 Cytochrom_C552 [H]

EC number: =1.7.2.2 [H]

Molecular weight: Translated: 55517; Mature: 55517

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAHYKTSIAISLIVIIVLVILRTTVTGQDSIKFIDDTDDQPIDQQNSDSIDLRYSNEYQ
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCEEEEECCHHH
SWKQTSDTTFRSLFNGNQQADILAEHPGMMVLWAGYAFSKDYLSPRGHMYSIEDLYKSLR
HHHHCCHHHHHHHHCCCCCCCHHCCCCCEEEEEECCHHHHHHCCCCCCEEEHHHHHHHHH
TGAPMTPTSGPQPAACWACKSPDIPRLLTTTDAKTLYKKKWAELGNEIVNPIGCADCHEP
CCCCCCCCCCCCCCEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCH
QSMGLRVTRSFLKSAYKRNGLRIEDATEQEMRSLVCAQCHAEYYFQGEDRILTLPWDQGY
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCEEEEECCCCCC
TVENIEAYYDSINFTDFTHKLSRAHLIKAQHPDFELFQMGIHGQRGVSCGECHMPAVGED
CHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCC
ENRYNNHHIKSPLAMIDRTCQACHRESEETLRKDVYERQNKVYAIRMRVEEELTKAHIEA
CCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHE
KFAWDKGATESQMKNVLKLLRQAQWRWDFAVASHGAAFHAPQEVTRILGSGLDKATQARI
EEECCCCCCHHHHHHHHHHHHHHHCCEEHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHH
QIMKVLAQLGYTQDVPMPDISTKAKAQQYIGLDMEAEQQAKQKFLETVIPQWQEEARANK
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
RFIEGN
CCCCCC
>Mature Secondary Structure
MKAHYKTSIAISLIVIIVLVILRTTVTGQDSIKFIDDTDDQPIDQQNSDSIDLRYSNEYQ
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCEEEEECCHHH
SWKQTSDTTFRSLFNGNQQADILAEHPGMMVLWAGYAFSKDYLSPRGHMYSIEDLYKSLR
HHHHCCHHHHHHHHCCCCCCCHHCCCCCEEEEEECCHHHHHHCCCCCCEEEHHHHHHHHH
TGAPMTPTSGPQPAACWACKSPDIPRLLTTTDAKTLYKKKWAELGNEIVNPIGCADCHEP
CCCCCCCCCCCCCCEEEECCCCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCH
QSMGLRVTRSFLKSAYKRNGLRIEDATEQEMRSLVCAQCHAEYYFQGEDRILTLPWDQGY
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCEEEEECCCCCC
TVENIEAYYDSINFTDFTHKLSRAHLIKAQHPDFELFQMGIHGQRGVSCGECHMPAVGED
CHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCC
ENRYNNHHIKSPLAMIDRTCQACHRESEETLRKDVYERQNKVYAIRMRVEEELTKAHIEA
CCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHE
KFAWDKGATESQMKNVLKLLRQAQWRWDFAVASHGAAFHAPQEVTRILGSGLDKATQARI
EEECCCCCCHHHHHHHHHHHHHHHCCEEHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHH
QIMKVLAQLGYTQDVPMPDISTKAKAQQYIGLDMEAEQQAKQKFLETVIPQWQEEARANK
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
RFIEGN
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA