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The map label for this gene is ymfI [H]

Identifier: 150006998

GI number: 150006998

Start: 381575

End: 382366

Strand: Reverse

Name: ymfI [H]

Synonym: BDI_0334

Alternate gene names: 150006998

Gene position: 382366-381575 (Counterclockwise)

Preceding gene: 150006999

Following gene: 150006997

Centisome position: 7.95

GC content: 47.73

Gene sequence:

>792_bases
ATGGAAAAGAAATGGGCTTTAGTCACGGGGGCCAGTTCCGGAATAGGTCTCGCCTATGCCGAGGAACTAGCCTCACGAGG
CTATCAGTTAGTGATCGTCAGCAATGAGGAACAGGCGATCCGGGAGAAAGGTGAGTTTCTCTCCGAGAAATATGGTATAG
AAGTTTTGCCGCTTTATCGTGATTTGGCGATCCCCGGGGCGGCCAAGGAGCTATATGATACTTGCCAAGAGAAAAACATC
CCAATAGAGGTATTGGTCAATAACGCCGGTATGTTTTTTTTCTGCGAGACCTTACAAGCTAAGGCCAACATCGTCGAGAA
AATGCTTTACCTCCACGTGACCACTCCTACCCAGCTTTGTTATTATTTCGGGCAAGAGATGAAGAAACGGCGTCATGGAT
ATATCCTCAATATGTCATCCCTATCTTGTTGGCTACCTTATCCGGGAATTACGCTATACGCATCCACCAAGCGATACTTG
AAAAGCTTCTCCCGAGGCTTGCGTTCAGAGTTACTGGATTATGGAGTAAGCGTAACCGTACTTTGTCCCGGCGCCGTCGC
CACGAATTTATATAACCTAAGCGACAACTTAAAGACCTTAGCGATCCGCCTAGGTATCATGATGCGTCCGGAAAAACTGG
CTAAAAAAGGGATTAATGCCTTGTTTCACCACCGGGCTTCCCTATTACCGGGTCTCTTCAACAAAATATGCACACCCCTC
GTAATGCTCCTCCCCCACGGCCTTGTCCGTTGGATCATGCGAACCACGAAATTAGTGAAGCTAGATCGATAA

Upstream 100 bases:

>100_bases
GTATCGCCCGTATGTATAGCGAGGATATGGTCTGTCATAAGAACGTGAAAGGTCTTCCGGAATGTATCGTACGTGAAATC
AAAAGGCTTGAATATAAATA

Downstream 100 bases:

>100_bases
TCCGACAAATAACATGCGAAATATTTGTCACATCAAACAAAAATCGTATCTTTGCACCGCTTTTTAGCCCCGTACCGTGT
GATGGGAAATTAGGAAGCAA

Product: putative short-chain dehydrogenase

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MEKKWALVTGASSGIGLAYAEELASRGYQLVIVSNEEQAIREKGEFLSEKYGIEVLPLYRDLAIPGAAKELYDTCQEKNI
PIEVLVNNAGMFFFCETLQAKANIVEKMLYLHVTTPTQLCYYFGQEMKKRRHGYILNMSSLSCWLPYPGITLYASTKRYL
KSFSRGLRSELLDYGVSVTVLCPGAVATNLYNLSDNLKTLAIRLGIMMRPEKLAKKGINALFHHRASLLPGLFNKICTPL
VMLLPHGLVRWIMRTTKLVKLDR

Sequences:

>Translated_263_residues
MEKKWALVTGASSGIGLAYAEELASRGYQLVIVSNEEQAIREKGEFLSEKYGIEVLPLYRDLAIPGAAKELYDTCQEKNI
PIEVLVNNAGMFFFCETLQAKANIVEKMLYLHVTTPTQLCYYFGQEMKKRRHGYILNMSSLSCWLPYPGITLYASTKRYL
KSFSRGLRSELLDYGVSVTVLCPGAVATNLYNLSDNLKTLAIRLGIMMRPEKLAKKGINALFHHRASLLPGLFNKICTPL
VMLLPHGLVRWIMRTTKLVKLDR
>Mature_263_residues
MEKKWALVTGASSGIGLAYAEELASRGYQLVIVSNEEQAIREKGEFLSEKYGIEVLPLYRDLAIPGAAKELYDTCQEKNI
PIEVLVNNAGMFFFCETLQAKANIVEKMLYLHVTTPTQLCYYFGQEMKKRRHGYILNMSSLSCWLPYPGITLYASTKRYL
KSFSRGLRSELLDYGVSVTVLCPGAVATNLYNLSDNLKTLAIRLGIMMRPEKLAKKGINALFHHRASLLPGLFNKICTPL
VMLLPHGLVRWIMRTTKLVKLDR

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: COG0300

COG function: function code R; Short-chain dehydrogenases of various substrate specificities

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI7705855, Length=193, Percent_Identity=32.6424870466321, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI226371731, Length=207, Percent_Identity=32.8502415458937, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI4557649, Length=191, Percent_Identity=28.2722513089005, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI32483357, Length=194, Percent_Identity=27.319587628866, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI20149619, Length=193, Percent_Identity=27.4611398963731, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI7706318, Length=191, Percent_Identity=32.9842931937173, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI31542939, Length=191, Percent_Identity=29.3193717277487, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4503817, Length=223, Percent_Identity=28.6995515695067, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1787335, Length=198, Percent_Identity=28.7878787878788, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI87082160, Length=192, Percent_Identity=29.6875, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1787820, Length=184, Percent_Identity=28.2608695652174, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI2367175, Length=193, Percent_Identity=28.4974093264249, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1788459, Length=196, Percent_Identity=29.5918367346939, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082100, Length=189, Percent_Identity=28.042328042328, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25152911, Length=195, Percent_Identity=33.8461538461538, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17559552, Length=191, Percent_Identity=28.2722513089005, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71994604, Length=196, Percent_Identity=30.1020408163265, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17561402, Length=190, Percent_Identity=30.5263157894737, Blast_Score=81, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI212646214, Length=191, Percent_Identity=28.2722513089005, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17562908, Length=196, Percent_Identity=30.1020408163265, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17557668, Length=191, Percent_Identity=28.7958115183246, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71994600, Length=192, Percent_Identity=26.5625, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17562906, Length=196, Percent_Identity=29.5918367346939, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17560176, Length=190, Percent_Identity=27.8947368421053, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17531453, Length=197, Percent_Identity=28.9340101522843, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI193203115, Length=241, Percent_Identity=26.9709543568465, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI193203117, Length=241, Percent_Identity=26.9709543568465, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17508895, Length=197, Percent_Identity=26.3959390862944, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319635, Length=200, Percent_Identity=26.5, Blast_Score=72, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6322067, Length=196, Percent_Identity=27.0408163265306, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24640442, Length=263, Percent_Identity=28.5171102661597, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161076933, Length=197, Percent_Identity=32.48730964467, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24584748, Length=197, Percent_Identity=32.48730964467, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24584744, Length=198, Percent_Identity=29.7979797979798, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28574402, Length=198, Percent_Identity=29.7979797979798, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24584752, Length=222, Percent_Identity=28.3783783783784, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28571526, Length=193, Percent_Identity=31.0880829015544, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355631, Length=168, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21357041, Length=189, Percent_Identity=29.1005291005291, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24649181, Length=187, Percent_Identity=27.807486631016, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI116007236, Length=206, Percent_Identity=25.7281553398058, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI21358495, Length=276, Percent_Identity=24.6376811594203, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 29705; Mature: 29705

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKWALVTGASSGIGLAYAEELASRGYQLVIVSNEEQAIREKGEFLSEKYGIEVLPLYR
CCCCEEEEECCCCCCCHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHCCCEEEEHHH
DLAIPGAAKELYDTCQEKNIPIEVLVNNAGMFFFCETLQAKANIVEKMLYLHVTTPTQLC
HHCCCCCHHHHHHHHHHCCCCEEEEEECCCEEEEHHHHHHHHHHHHHHHHHEECCHHHHH
YYFGQEMKKRRHGYILNMSSLSCWLPYPGITLYASTKRYLKSFSRGLRSELLDYGVSVTV
HHHHHHHHHHHCCEEEEECCCEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCEEEE
LCPGAVATNLYNLSDNLKTLAIRLGIMMRPEKLAKKGINALFHHRASLLPGLFNKICTPL
EECCHHHHHHHHCCCHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMLLPHGLVRWIMRTTKLVKLDR
HHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEKKWALVTGASSGIGLAYAEELASRGYQLVIVSNEEQAIREKGEFLSEKYGIEVLPLYR
CCCCEEEEECCCCCCCHHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHCCCEEEEHHH
DLAIPGAAKELYDTCQEKNIPIEVLVNNAGMFFFCETLQAKANIVEKMLYLHVTTPTQLC
HHCCCCCHHHHHHHHHHCCCCEEEEEECCCEEEEHHHHHHHHHHHHHHHHHEECCHHHHH
YYFGQEMKKRRHGYILNMSSLSCWLPYPGITLYASTKRYLKSFSRGLRSELLDYGVSVTV
HHHHHHHHHHHCCEEEEECCCEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCEEEE
LCPGAVATNLYNLSDNLKTLAIRLGIMMRPEKLAKKGINALFHHRASLLPGLFNKICTPL
EECCHHHHHHHHCCCHHHHHHHHHHHEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VMLLPHGLVRWIMRTTKLVKLDR
HHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]