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The map label for this gene is lacA [C]

Identifier: 150006655

GI number: 150006655

Start: 5146074

End: 5146649

Strand: Direct

Name: lacA [C]

Synonym: BVU_4176

Alternate gene names: 150006655

Gene position: 5146074-5146649 (Clockwise)

Preceding gene: 150006654

Following gene: 150006656

Centisome position: 99.67

GC content: 52.26

Gene sequence:

>576_bases
ATGACATTAGATGATTTTTTGAATCATATTGAAGCCGGCGGTGCACTCAATACACCGGAAATCTATCGGCTGATGGATGA
AATGAGCGATGAAGCCCGGCGCGTTACCTGTGAGATAAACAACTCGTACCATTCACAAGAAGAATTGCGTGCGTTCATGT
CGCGCCTGACCGGTAAACCTGTGGACGAAACATTCAAGATGTTTCCTCCGTTCTACACAGACTTCGGCAAGAATATTACC
ATCGGGCGTCGTGTCTTTATCAATGCCGGTTGCCACTTCCAGGATCATGGCGGTGTGACGTTGGGTGACGGCTGTCTTAT
CGGGCATAATGTGGTCTTTGCCACGCTCGATCACGGTACGGCTCCCGAAGACCGCGGGGCAATGTATCCGGCGCCTATCC
GGTTAGGCAAAAATGTATGGGTAGGCTCCAACTCCACCATCCTGCGGGGTGTGACCGTGGGTGACAACGCCATTATCGCC
GCCGGATCTGTGGTGACGAAAGACGTAGCGGCCAACACGGTTGTAGGAGGTGTACCGGCAAGGCATATCCGAGATATCGA
CAGGAACGAAAAGTGA

Upstream 100 bases:

>100_bases
TGTTTCGCTGTGGTGTATGGCACATGGAAGAAATCAAAGGAAACCCGAAATCACAAGAGGCATACAAAACGAGTAAACAA
GTATAGGAAAAAAGAATATT

Downstream 100 bases:

>100_bases
TAGCCTATGGAATCGGAATCACTAACCAACCATACTGCCATCCTTGTCAGATCCATCCGAACGGTACACCATACTGAACG
GGTTGTGCGGATTGGTTCTA

Product: acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 191; Mature: 190

Protein sequence:

>191_residues
MTLDDFLNHIEAGGALNTPEIYRLMDEMSDEARRVTCEINNSYHSQEELRAFMSRLTGKPVDETFKMFPPFYTDFGKNIT
IGRRVFINAGCHFQDHGGVTLGDGCLIGHNVVFATLDHGTAPEDRGAMYPAPIRLGKNVWVGSNSTILRGVTVGDNAIIA
AGSVVTKDVAANTVVGGVPARHIRDIDRNEK

Sequences:

>Translated_191_residues
MTLDDFLNHIEAGGALNTPEIYRLMDEMSDEARRVTCEINNSYHSQEELRAFMSRLTGKPVDETFKMFPPFYTDFGKNIT
IGRRVFINAGCHFQDHGGVTLGDGCLIGHNVVFATLDHGTAPEDRGAMYPAPIRLGKNVWVGSNSTILRGVTVGDNAIIA
AGSVVTKDVAANTVVGGVPARHIRDIDRNEK
>Mature_190_residues
TLDDFLNHIEAGGALNTPEIYRLMDEMSDEARRVTCEINNSYHSQEELRAFMSRLTGKPVDETFKMFPPFYTDFGKNITI
GRRVFINAGCHFQDHGGVTLGDGCLIGHNVVFATLDHGTAPEDRGAMYPAPIRLGKNVWVGSNSTILRGVTVGDNAIIAA
GSVVTKDVAANTVVGGVPARHIRDIDRNEK

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=169, Percent_Identity=39.6449704142012, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI1786664, Length=174, Percent_Identity=37.9310344827586, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI1788345, Length=118, Percent_Identity=29.6610169491525, Blast_Score=60, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322243, Length=162, Percent_Identity=34.5679012345679, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 20877; Mature: 20746

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDDFLNHIEAGGALNTPEIYRLMDEMSDEARRVTCEINNSYHSQEELRAFMSRLTGKP
CCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCC
VDETFKMFPPFYTDFGKNITIGRRVFINAGCHFQDHGGVTLGDGCLIGHNVVFATLDHGT
HHHHHHHCCCHHHHCCCCEEECEEEEEECCCEEECCCCEEECCCEEEECCEEEEEECCCC
APEDRGAMYPAPIRLGKNVWVGSNSTILRGVTVGDNAIIAAGSVVTKDVAANTVVGGVPA
CCCCCCCCCCCCEEECCEEEECCCCEEEEEEEECCCEEEEECCHHHHHHHHHHEECCCCH
RHIRDIDRNEK
HHHHHCCCCCC
>Mature Secondary Structure 
TLDDFLNHIEAGGALNTPEIYRLMDEMSDEARRVTCEINNSYHSQEELRAFMSRLTGKP
CHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCC
VDETFKMFPPFYTDFGKNITIGRRVFINAGCHFQDHGGVTLGDGCLIGHNVVFATLDHGT
HHHHHHHCCCHHHHCCCCEEECEEEEEECCCEEECCCCEEECCCEEEECCEEEEEECCCC
APEDRGAMYPAPIRLGKNVWVGSNSTILRGVTVGDNAIIAAGSVVTKDVAANTVVGGVPA
CCCCCCCCCCCCEEECCEEEECCCCEEEEEEEECCCEEEEECCHHHHHHHHHHEECCCCH
RHIRDIDRNEK
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA