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The map label for this gene is lacZ [H]

Identifier: 150006614

GI number: 150006614

Start: 5084522

End: 5087026

Strand: Reverse

Name: lacZ [H]

Synonym: BVU_4134

Alternate gene names: 150006614

Gene position: 5087026-5084522 (Counterclockwise)

Preceding gene: 150006615

Following gene: 150006613

Centisome position: 98.52

GC content: 44.99

Gene sequence:

>2505_bases
ATGAATACGAAACGATTTTTGTGTGCTGCGTTAGGAGCTGTTTGTTATTTTGCTTTTTTGCAAGCGCAGGTACGTACAGA
GCAGACTTTCGAAAAAGGATGGAAATTTACGCGTGAAGATAATGCGGAATTTGCCAATCCCGGTTATAATGACAGTAAAT
GGCAGAACGTCACCGTTCCTCATGATTGGGCGATTTATGGTCCGTTCAGTATCAATAACGACAAGCAGGAAATGGCTATT
ACTCAAGATGGACAGACCGAGGCGATGGAACATGCCGGACGTACCGGAGGTCTTCCGTTTGTAGGAACCGGATGGTATCG
CCTGAACTTTGATGCTCCCGGTTTTGAGAAAGGCAAGAAAGCGACTTTGATATTTGATGGAGCAATGAGCCATGCCCGTG
TTTATGTGAACGGGCAGGAGGCCGGTTATTGGCCCTACGGTTATAATTCTTTTTATGTGGATGCGACCCCTTATCTGAAA
CCGGGTGAGAGGAACGAACTGGCAGTCCGTTTGGAAAACGAGCCTGAATCATCACGTTGGTATCCGGGGGCCGGATTGTA
CCGCAATGTTCATCTGGTAATCAATGAAGATACGCATATTCCTGCTTGGGGGACGTATGTTACCACTCCTGTGGTGACAG
ACAAATATGCAAAAGTGTCTTTGAAAACTTCTTTGGTTAGCCCTGAAGGAGCTAATAAGGATAATTACCGTATTGTGACG
CAGATCAAGGATAAAAACGGCAAAGTGGTTGCTACCGGGGAAAACAAGTTGAGTGTTTTTGATAATGCTTTGTTTGAACA
GGAGTTTGCAGTGGCTAATCCTGAGTTGTGGAGTCCTGATACTCCGGTTCTTTATACAGCTGAATCCAAAGTATATGAAG
GGAATACCTTGAAAGACGAATATACCACCCGCTTTGGTATCCGTACTTTGGAGATTGTTCCCGGCAAGGGCTTTTTCCTG
AATGGAAAGCTGACCAAATTCAAAGGGGTATGTAACCATCATGACTTGGGTCCGTTAGGCGGTGCTGTAAATGATGCCGC
TATACGTCGTCAGATTCGTATTCTGAAAGATATGGGTTGTAATGCCATCCGTACTTCTCATAATATGCCGGCACCCGAAT
TGGTGGAGGCTTGTGATGAAATGGGTATGATGCTGATGGTGGAGTCTTTCGACGAGTGGAAATCGGCAAAGATGGCCAAC
GGTTATCATAAAATATTTGATGAATGGGTGGAGAAGGATCTGACTAATCTGATCCGTCATTACCGTAACAATCCGAGTAT
TGTTATGTGGTGCATCGGTAACGAAGTACCCGACCAGTGGAATGGCAATAACGGACCAAAGCTTTCGCGTATGTTACAGG
ATATCTGTCATCGTGAAGATCCTACCCGTCCTGTAACACAAGGTATGGATGCGCCTGATGCTGTAGTAAATAATAATATG
GCTGCTGTGATGGATGTGGCCGGTTTCAATTACCGCCCTCATAAATATCCAGAAAACTATAAGAAATTGCCTCAGCAGAT
TATTCTAGGTAGTGAAACAGCTTCTACAGTAAGTTCGCGTGGCGTCTATAAATTCCCTGTTGTCCGTCAGGCTATGAAGA
AATATGACGATCATCAGTCTTCTTCTTACGATGTGGAACATTGTGGTTGGTCCAATTTGCCGGAGGATGACTGGATTTGG
CATGAGGATAACGCATGGGGTATCGGAGAATTTGTATGGACGGGCTTCGATTATTTGGGCGAACCGACTCCTTATTATAC
TGATTGGCCCAGCCATTCTTCTTTGTTCGGTATCATCGACCTGGCCGGATTGCCCAAAGACCGTTACTATCTGTACCGCA
GTCATTGGAACAAAGATGAAGAAACGCTGCATATCCTTCCTCATTGGACATGGCCGGGACGTGAAGGAGAAGTGACTCCT
ATCTTTGTTTATACAAATTATCCGTCGGCAGAAGTGTTTATCAATGGCAAGAGTCAGGGTAAGCGTACCAAAGATTTGAC
TGTAACGGCTGAAAATAGTGCGGATTCTGCTTCCATAGCAGATTTCAAACGCCAGAAACGCTACCGTTTGATGTGGATGG
ATACAAAGTATGAGCCGGGAACCGTAAAAGTGGTTGCCTATAATGATAAGGGGGAAGCTGTGGCCGAAAAGGAAATCCAT
ACGGCAGGCAAGCCCGACCATATAGAACTGGTTACAGATCGCAATGAAATAAAGGCTGATGGCAAGGATTTGTCATTTGT
CACAGTAAGAGTGGTGGACAAAGAGGGTAATCTTTGTCCCGATGCGCAGCATCTCATCAAGTATTCTGTAAAAGGTGCGG
GAACCTATCGTGCCGGTGCAAACGGAGATCCCACTTCATTGGAACTTTTCCATGTTCCGCAGATGAAAGTGTTTAACGGA
ATGATGACTGCTGTTGTGCAAAGTACTGATAAACCGGGAGAAATCATATTGACCGCAACAGGTAAAGGACTTAAATCAGG
TAGGCTAGTTTTAATGAGTAAGTAA

Upstream 100 bases:

>100_bases
GCAATAAGAAGATTAATAAATATTTGATTTCGCATGATGATTTGATCAAGGCAGGTGAAATTACTTTTATAGTAACAGAA
AATCCTAATAAGAAATAACT

Downstream 100 bases:

>100_bases
AAAGATTTGTTTCAATTTCCACTATCTGTGCCCCGCTTGATTTTATTAAGCGGGGCTTATTTATTAAAAAAAGTTGGAAA
ATAATTGGGGAAATCTGTTT

Product: beta-galactosidase

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 834; Mature: 834

Protein sequence:

>834_residues
MNTKRFLCAALGAVCYFAFLQAQVRTEQTFEKGWKFTREDNAEFANPGYNDSKWQNVTVPHDWAIYGPFSINNDKQEMAI
TQDGQTEAMEHAGRTGGLPFVGTGWYRLNFDAPGFEKGKKATLIFDGAMSHARVYVNGQEAGYWPYGYNSFYVDATPYLK
PGERNELAVRLENEPESSRWYPGAGLYRNVHLVINEDTHIPAWGTYVTTPVVTDKYAKVSLKTSLVSPEGANKDNYRIVT
QIKDKNGKVVATGENKLSVFDNALFEQEFAVANPELWSPDTPVLYTAESKVYEGNTLKDEYTTRFGIRTLEIVPGKGFFL
NGKLTKFKGVCNHHDLGPLGGAVNDAAIRRQIRILKDMGCNAIRTSHNMPAPELVEACDEMGMMLMVESFDEWKSAKMAN
GYHKIFDEWVEKDLTNLIRHYRNNPSIVMWCIGNEVPDQWNGNNGPKLSRMLQDICHREDPTRPVTQGMDAPDAVVNNNM
AAVMDVAGFNYRPHKYPENYKKLPQQIILGSETASTVSSRGVYKFPVVRQAMKKYDDHQSSSYDVEHCGWSNLPEDDWIW
HEDNAWGIGEFVWTGFDYLGEPTPYYTDWPSHSSLFGIIDLAGLPKDRYYLYRSHWNKDEETLHILPHWTWPGREGEVTP
IFVYTNYPSAEVFINGKSQGKRTKDLTVTAENSADSASIADFKRQKRYRLMWMDTKYEPGTVKVVAYNDKGEAVAEKEIH
TAGKPDHIELVTDRNEIKADGKDLSFVTVRVVDKEGNLCPDAQHLIKYSVKGAGTYRAGANGDPTSLELFHVPQMKVFNG
MMTAVVQSTDKPGEIILTATGKGLKSGRLVLMSK

Sequences:

>Translated_834_residues
MNTKRFLCAALGAVCYFAFLQAQVRTEQTFEKGWKFTREDNAEFANPGYNDSKWQNVTVPHDWAIYGPFSINNDKQEMAI
TQDGQTEAMEHAGRTGGLPFVGTGWYRLNFDAPGFEKGKKATLIFDGAMSHARVYVNGQEAGYWPYGYNSFYVDATPYLK
PGERNELAVRLENEPESSRWYPGAGLYRNVHLVINEDTHIPAWGTYVTTPVVTDKYAKVSLKTSLVSPEGANKDNYRIVT
QIKDKNGKVVATGENKLSVFDNALFEQEFAVANPELWSPDTPVLYTAESKVYEGNTLKDEYTTRFGIRTLEIVPGKGFFL
NGKLTKFKGVCNHHDLGPLGGAVNDAAIRRQIRILKDMGCNAIRTSHNMPAPELVEACDEMGMMLMVESFDEWKSAKMAN
GYHKIFDEWVEKDLTNLIRHYRNNPSIVMWCIGNEVPDQWNGNNGPKLSRMLQDICHREDPTRPVTQGMDAPDAVVNNNM
AAVMDVAGFNYRPHKYPENYKKLPQQIILGSETASTVSSRGVYKFPVVRQAMKKYDDHQSSSYDVEHCGWSNLPEDDWIW
HEDNAWGIGEFVWTGFDYLGEPTPYYTDWPSHSSLFGIIDLAGLPKDRYYLYRSHWNKDEETLHILPHWTWPGREGEVTP
IFVYTNYPSAEVFINGKSQGKRTKDLTVTAENSADSASIADFKRQKRYRLMWMDTKYEPGTVKVVAYNDKGEAVAEKEIH
TAGKPDHIELVTDRNEIKADGKDLSFVTVRVVDKEGNLCPDAQHLIKYSVKGAGTYRAGANGDPTSLELFHVPQMKVFNG
MMTAVVQSTDKPGEIILTATGKGLKSGRLVLMSK
>Mature_834_residues
MNTKRFLCAALGAVCYFAFLQAQVRTEQTFEKGWKFTREDNAEFANPGYNDSKWQNVTVPHDWAIYGPFSINNDKQEMAI
TQDGQTEAMEHAGRTGGLPFVGTGWYRLNFDAPGFEKGKKATLIFDGAMSHARVYVNGQEAGYWPYGYNSFYVDATPYLK
PGERNELAVRLENEPESSRWYPGAGLYRNVHLVINEDTHIPAWGTYVTTPVVTDKYAKVSLKTSLVSPEGANKDNYRIVT
QIKDKNGKVVATGENKLSVFDNALFEQEFAVANPELWSPDTPVLYTAESKVYEGNTLKDEYTTRFGIRTLEIVPGKGFFL
NGKLTKFKGVCNHHDLGPLGGAVNDAAIRRQIRILKDMGCNAIRTSHNMPAPELVEACDEMGMMLMVESFDEWKSAKMAN
GYHKIFDEWVEKDLTNLIRHYRNNPSIVMWCIGNEVPDQWNGNNGPKLSRMLQDICHREDPTRPVTQGMDAPDAVVNNNM
AAVMDVAGFNYRPHKYPENYKKLPQQIILGSETASTVSSRGVYKFPVVRQAMKKYDDHQSSSYDVEHCGWSNLPEDDWIW
HEDNAWGIGEFVWTGFDYLGEPTPYYTDWPSHSSLFGIIDLAGLPKDRYYLYRSHWNKDEETLHILPHWTWPGREGEVTP
IFVYTNYPSAEVFINGKSQGKRTKDLTVTAENSADSASIADFKRQKRYRLMWMDTKYEPGTVKVVAYNDKGEAVAEKEIH
TAGKPDHIELVTDRNEIKADGKDLSFVTVRVVDKEGNLCPDAQHLIKYSVKGAGTYRAGANGDPTSLELFHVPQMKVFNG
MMTAVVQSTDKPGEIILTATGKGLKSGRLVLMSK

Specific function: Unknown

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=554, Percent_Identity=24.3682310469314, Blast_Score=94, Evalue=4e-19,
Organism=Escherichia coli, GI1787903, Length=412, Percent_Identity=26.9417475728155, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI48994920, Length=404, Percent_Identity=25, Blast_Score=109, Evalue=6e-25,
Organism=Escherichia coli, GI1786539, Length=401, Percent_Identity=27.431421446384, Blast_Score=90, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17510825, Length=430, Percent_Identity=25.3488372093023, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24651745, Length=523, Percent_Identity=25.4302103250478, Blast_Score=117, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24655438, Length=527, Percent_Identity=25.6166982922201, Blast_Score=114, Evalue=4e-25,
Organism=Drosophila melanogaster, GI19922582, Length=527, Percent_Identity=25.6166982922201, Blast_Score=113, Evalue=5e-25,
Organism=Drosophila melanogaster, GI62471735, Length=527, Percent_Identity=25.6166982922201, Blast_Score=113, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 94147; Mature: 94147

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTKRFLCAALGAVCYFAFLQAQVRTEQTFEKGWKFTREDNAEFANPGYNDSKWQNVTVP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEECCC
HDWAIYGPFSINNDKQEMAITQDGQTEAMEHAGRTGGLPFVGTGWYRLNFDAPGFEKGKK
CCEEEEECEECCCCHHHEEEECCCCHHHHHHCCCCCCCCEEECCEEEEECCCCCCCCCCE
ATLIFDGAMSHARVYVNGQEAGYWPYGYNSFYVDATPYLKPGERNELAVRLENEPESSRW
EEEEEECCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCCCCC
YPGAGLYRNVHLVINEDTHIPAWGTYVTTPVVTDKYAKVSLKTSLVSPEGANKDNYRIVT
CCCCCCEEEEEEEEECCCCCCCCCCEEECCEECCCEEEEEEEEEEECCCCCCCCCEEEEE
QIKDKNGKVVATGENKLSVFDNALFEQEFAVANPELWSPDTPVLYTAESKVYEGNTLKDE
EEECCCCEEEEECCCCEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCEEECCCCCCHH
YTTRFGIRTLEIVPGKGFFLNGKLTKFKGVCNHHDLGPLGGAVNDAAIRRQIRILKDMGC
HHHEECCEEEEEECCCCEEECCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
NAIRTSHNMPAPELVEACDEMGMMLMVESFDEWKSAKMANGYHKIFDEWVEKDLTNLIRH
CEEECCCCCCCHHHHHHHHHCCEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
YRNNPSIVMWCIGNEVPDQWNGNNGPKLSRMLQDICHREDPTRPVTQGMDAPDAVVNNNM
HCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHCCCCCCHHHHCCCC
AAVMDVAGFNYRPHKYPENYKKLPQQIILGSETASTVSSRGVYKFPVVRQAMKKYDDHQS
EEEEEECCCCCCCCCCCHHHHHCCHHHEECCCHHHHHHHCCCEECHHHHHHHHHHCCCCC
SSYDVEHCGWSNLPEDDWIWHEDNAWGIGEFVWTGFDYLGEPTPYYTDWPSHSSLFGIID
CCCCHHHCCCCCCCCCCCEEECCCCCCCHHHHHHCHHHCCCCCCCCCCCCCCCCEEEEEE
LAGLPKDRYYLYRSHWNKDEETLHILPHWTWPGREGEVTPIFVYTNYPSAEVFINGKSQG
ECCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCCEEEEECCCCCC
KRTKDLTVTAENSADSASIADFKRQKRYRLMWMDTKYEPGTVKVVAYNDKGEAVAEKEIH
CCCCEEEEEECCCCCCCHHHHHHHCCCEEEEEEECCCCCCEEEEEEECCCCCCHHHHHHH
TAGKPDHIELVTDRNEIKADGKDLSFVTVRVVDKEGNLCPDAQHLIKYSVKGAGTYRAGA
CCCCCCEEEEEECCHHHCCCCCCEEEEEEEEEECCCCCCCCHHHHHEEEECCCCEECCCC
NGDPTSLELFHVPQMKVFNGMMTAVVQSTDKPGEIILTATGKGLKSGRLVLMSK
CCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEEC
>Mature Secondary Structure
MNTKRFLCAALGAVCYFAFLQAQVRTEQTFEKGWKFTREDNAEFANPGYNDSKWQNVTVP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEECCC
HDWAIYGPFSINNDKQEMAITQDGQTEAMEHAGRTGGLPFVGTGWYRLNFDAPGFEKGKK
CCEEEEECEECCCCHHHEEEECCCCHHHHHHCCCCCCCCEEECCEEEEECCCCCCCCCCE
ATLIFDGAMSHARVYVNGQEAGYWPYGYNSFYVDATPYLKPGERNELAVRLENEPESSRW
EEEEEECCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCCCCC
YPGAGLYRNVHLVINEDTHIPAWGTYVTTPVVTDKYAKVSLKTSLVSPEGANKDNYRIVT
CCCCCCEEEEEEEEECCCCCCCCCCEEECCEECCCEEEEEEEEEEECCCCCCCCCEEEEE
QIKDKNGKVVATGENKLSVFDNALFEQEFAVANPELWSPDTPVLYTAESKVYEGNTLKDE
EEECCCCEEEEECCCCEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCEEECCCCCCHH
YTTRFGIRTLEIVPGKGFFLNGKLTKFKGVCNHHDLGPLGGAVNDAAIRRQIRILKDMGC
HHHEECCEEEEEECCCCEEECCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
NAIRTSHNMPAPELVEACDEMGMMLMVESFDEWKSAKMANGYHKIFDEWVEKDLTNLIRH
CEEECCCCCCCHHHHHHHHHCCEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
YRNNPSIVMWCIGNEVPDQWNGNNGPKLSRMLQDICHREDPTRPVTQGMDAPDAVVNNNM
HCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHCCCCCCHHHHCCCC
AAVMDVAGFNYRPHKYPENYKKLPQQIILGSETASTVSSRGVYKFPVVRQAMKKYDDHQS
EEEEEECCCCCCCCCCCHHHHHCCHHHEECCCHHHHHHHCCCEECHHHHHHHHHHCCCCC
SSYDVEHCGWSNLPEDDWIWHEDNAWGIGEFVWTGFDYLGEPTPYYTDWPSHSSLFGIID
CCCCHHHCCCCCCCCCCCEEECCCCCCCHHHHHHCHHHCCCCCCCCCCCCCCCCEEEEEE
LAGLPKDRYYLYRSHWNKDEETLHILPHWTWPGREGEVTPIFVYTNYPSAEVFINGKSQG
ECCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCEEEEEEEECCCCEEEEECCCCCC
KRTKDLTVTAENSADSASIADFKRQKRYRLMWMDTKYEPGTVKVVAYNDKGEAVAEKEIH
CCCCEEEEEECCCCCCCHHHHHHHCCCEEEEEEECCCCCCEEEEEEECCCCCCHHHHHHH
TAGKPDHIELVTDRNEIKADGKDLSFVTVRVVDKEGNLCPDAQHLIKYSVKGAGTYRAGA
CCCCCCEEEEEECCHHHCCCCCCEEEEEEEEEECCCCCCCCHHHHHEEEECCCCEECCCC
NGDPTSLELFHVPQMKVFNGMMTAVVQSTDKPGEIILTATGKGLKSGRLVLMSK
CCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA