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The map label for this gene is nanA [H]

Identifier: 150006597

GI number: 150006597

Start: 5064221

End: 5065135

Strand: Direct

Name: nanA [H]

Synonym: BVU_4117

Alternate gene names: 150006597

Gene position: 5064221-5065135 (Clockwise)

Preceding gene: 150006590

Following gene: 150006598

Centisome position: 98.08

GC content: 44.81

Gene sequence:

>915_bases
ATGGAAAAGATTCACGGATTAATTGACGCACCTTTTACTCCCTTTTATGAGAACGGAGAAGTAAATTATGAACCTATTGA
AGCCTATTGCCAACTATTGGTTAGAAACGGACTTCAAGGCGTATTTATCAATGGTTCTTCCGGAGAAGGCTATATGCTGA
CAGAAGACGAACGCATGAAACTGGCTGAACGCTGGATGGAAGTAGCACCCGAAGGATTCAAAGTCATTGTCCATGTAGGA
AGCACTTGTGTAAAATCAAGCAAACGTCTAGCAGAACACGCTCAGAAAATCGGAGCATGGGGTATCGGAGCAATGGCACC
TCCTTTCCCCAAAGTAGGACGCATTGAAGAATTGGTGAAATACTGCGAAGAAATAGCATGCGGCGCTCCTGCACTTCCTT
TCTATTTTTATCATATCCCAGCTTTCAACGGAGCATTCCTCTCAATGGTAGCATTCTTGGAAGCCGTAGACGGTCGCATT
CCTAATTTCGCCGGTATCAAATATACTTTCGAAAGCCTGTATGAATACAACCAATGCCGTTTATATAAAAATGGCAAATT
TGACATGTTGCACGGCCAGGATGAAACGATCCTTCCTTGTCTGGCTATGGGCGGTGCACAGGGCGGTATCGGTGGAACAA
CCAACTATAACGGCAAAGAACTCACAGGTATTATCGAAGCATGGAAAGCCGGTGATCTGGAACTGGCACGCGAACGCCAG
AATTTCGCACAGGAAGTTATCAATGTCATCTGTCACTATCGTGGAAACATCGTTGGCGGTAAACGCATCATGAAACTGAT
CGGGCTGGATTTAGGAAAGAACCGGACTCCGTTCCAGAACATGACAGATGAGGAAGAAGCTGCCATGAAAGCCGAACTGG
AAGCAATCAATTTCTTCGAAAGATGTAACAAATAA

Upstream 100 bases:

>100_bases
TAAATTTAGTTTTTACTTGTTGTTTTCATAAATCATTTATATATTTGCACGCAACATGATTAAGTAATATAAAATATTCA
TTTCAAATAAATTAAAGGCT

Downstream 100 bases:

>100_bases
ACCTGAACCACGATGTTTTCAATAGACTATATAAAAGAATGGTCGGAATCATACAAAACAGATCTGACCACCAATATCAT
GCCGTTTTGGCTGAAATACG

Product: N-acetylneuraminate lyase

Products: NA

Alternate protein names: N-acetylneuraminate pyruvate-lyase; N-acetylneuraminic acid aldolase; Sialate lyase; Sialic acid aldolase; Sialic acid lyase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MEKIHGLIDAPFTPFYENGEVNYEPIEAYCQLLVRNGLQGVFINGSSGEGYMLTEDERMKLAERWMEVAPEGFKVIVHVG
STCVKSSKRLAEHAQKIGAWGIGAMAPPFPKVGRIEELVKYCEEIACGAPALPFYFYHIPAFNGAFLSMVAFLEAVDGRI
PNFAGIKYTFESLYEYNQCRLYKNGKFDMLHGQDETILPCLAMGGAQGGIGGTTNYNGKELTGIIEAWKAGDLELARERQ
NFAQEVINVICHYRGNIVGGKRIMKLIGLDLGKNRTPFQNMTDEEEAAMKAELEAINFFERCNK

Sequences:

>Translated_304_residues
MEKIHGLIDAPFTPFYENGEVNYEPIEAYCQLLVRNGLQGVFINGSSGEGYMLTEDERMKLAERWMEVAPEGFKVIVHVG
STCVKSSKRLAEHAQKIGAWGIGAMAPPFPKVGRIEELVKYCEEIACGAPALPFYFYHIPAFNGAFLSMVAFLEAVDGRI
PNFAGIKYTFESLYEYNQCRLYKNGKFDMLHGQDETILPCLAMGGAQGGIGGTTNYNGKELTGIIEAWKAGDLELARERQ
NFAQEVINVICHYRGNIVGGKRIMKLIGLDLGKNRTPFQNMTDEEEAAMKAELEAINFFERCNK
>Mature_304_residues
MEKIHGLIDAPFTPFYENGEVNYEPIEAYCQLLVRNGLQGVFINGSSGEGYMLTEDERMKLAERWMEVAPEGFKVIVHVG
STCVKSSKRLAEHAQKIGAWGIGAMAPPFPKVGRIEELVKYCEEIACGAPALPFYFYHIPAFNGAFLSMVAFLEAVDGRI
PNFAGIKYTFESLYEYNQCRLYKNGKFDMLHGQDETILPCLAMGGAQGGIGGTTNYNGKELTGIIEAWKAGDLELARERQ
NFAQEVINVICHYRGNIVGGKRIMKLIGLDLGKNRTPFQNMTDEEEAAMKAELEAINFFERCNK

Specific function: Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate [H]

COG id: COG0329

COG function: function code EM; Dihydrodipicolinate synthase/N-acetylneuraminate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DHDPS family. NanA subfamily [H]

Homologues:

Organism=Homo sapiens, GI13540533, Length=306, Percent_Identity=36.6013071895425, Blast_Score=207, Evalue=9e-54,
Organism=Homo sapiens, GI31543060, Length=296, Percent_Identity=24.6621621621622, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1789620, Length=250, Percent_Identity=29.6, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI87082415, Length=241, Percent_Identity=29.4605809128631, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786463, Length=234, Percent_Identity=28.6324786324786, Blast_Score=92, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR002220
- InterPro:   IPR020625
- InterPro:   IPR005264 [H]

Pfam domain/function: PF00701 DHDPS [H]

EC number: =4.1.3.3 [H]

Molecular weight: Translated: 33909; Mature: 33909

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKIHGLIDAPFTPFYENGEVNYEPIEAYCQLLVRNGLQGVFINGSSGEGYMLTEDERMK
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEECCHHHHH
LAERWMEVAPEGFKVIVHVGSTCVKSSKRLAEHAQKIGAWGIGAMAPPFPKVGRIEELVK
HHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
YCEEIACGAPALPFYFYHIPAFNGAFLSMVAFLEAVDGRIPNFAGIKYTFESLYEYNQCR
HHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHEE
LYKNGKFDMLHGQDETILPCLAMGGAQGGIGGTTNYNGKELTGIIEAWKAGDLELARERQ
EEECCCEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHH
NFAQEVINVICHYRGNIVGGKRIMKLIGLDLGKNRTPFQNMTDEEEAAMKAELEAINFFE
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RCNK
HHCC
>Mature Secondary Structure
MEKIHGLIDAPFTPFYENGEVNYEPIEAYCQLLVRNGLQGVFINGSSGEGYMLTEDERMK
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEECCHHHHH
LAERWMEVAPEGFKVIVHVGSTCVKSSKRLAEHAQKIGAWGIGAMAPPFPKVGRIEELVK
HHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
YCEEIACGAPALPFYFYHIPAFNGAFLSMVAFLEAVDGRIPNFAGIKYTFESLYEYNQCR
HHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHEE
LYKNGKFDMLHGQDETILPCLAMGGAQGGIGGTTNYNGKELTGIIEAWKAGDLELARERQ
EEECCCEEEECCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHH
NFAQEVINVICHYRGNIVGGKRIMKLIGLDLGKNRTPFQNMTDEEEAAMKAELEAINFFE
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RCNK
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481431 [H]