Click here to switch to the map view.

The map label for this gene is amtB [C]

Identifier: 150006483

GI number: 150006483

Start: 4929676

End: 4931034

Strand: Reverse

Name: amtB [C]

Synonym: BVU_4002

Alternate gene names: 150006483

Gene position: 4931034-4929676 (Counterclockwise)

Preceding gene: 150006484

Following gene: 150006482

Centisome position: 95.5

GC content: 55.48

Gene sequence:

>1359_bases
ATGGATACTATACTCTTATTTATGCTCCCCGCCGGCCTTTGGGCGCAAGATGCAGGCGTGGCAGCCACCACCGCGGCGCC
CGATGCAACCGCCGGGGCCCTGGGCGAACTTGCTACCGGACTCAACACTGTATGGATGCTACTTGCTGCCATGCTGGTAT
TCTTCATGCAACCGGGATTCGCCTTGGTAGAAGCCGGGTTCATCCGTACCAAGAACACGGCCAACGTGCTGATGAAAAAT
CTGGTAGACTTCATGTTCGGCTCCATCCTGTTCTGGTTCATCGGCTTCGGGCTGATGTTCGGCATAGGCGGTTTTGTGGG
CGCCCCCCATTTTTTCAACTTGGAGGCGATGGACAAAATCATCGACAACGGATTGCCCATCGAAGGCTTCCTGATTTTCC
AGACTGTATTCTGCGCCACGGCGGCCACCATCGTGTCCGGAGCCATGGCGGAACGTACTAAGTTCTCCATGTATCTGGTC
TATACTGTCTTTATCAGCGTACTCATTTATCCGGTTTCCGGCCACTGGACCTGGGGCGGAGGATGGCTGATGAACGGAGA
CGAAGGCTCCTTTATGATGAGGACGTTCGGCGCTACTTTCCATGACTTTGCCGGCTCTACGGTGGTTCACTCCGTAGGCG
GATGGATTGCCCTGGTGGGCGCCGCCATTCTGGGACCGCGTATTGGCAAATACGGGAAAGACGGCAAATCAAGGGCCATC
CCCGGTCATAGTTTGACGCTTGCCTGCCTGGGCGTGTTTATCCTTTGGTTCGGCTGGTTCGGGTTCAACCCCGGTTCGCA
GTTGGCAGCGGCAACAAGTGGAGACCAGACAGCCATTTCGCATGTGTTCCTCACCACCAACCTGGCAGCTTGTGCAGGAG
GTTTCTTCGCACTGGTGGCGAGTTGGATGAAGTATGGCAAACCTTCCTTGTCCCTCACACTGAACGGTGTGCTGGCAGGA
CTGGTGGGCATCACGGCGGGATGTGACCTGGTATCGCCTTTCGGGTCTGTACTTATCGGTGCCATTTGCGGCGTGGTAAT
GATATTTGCAGTCGACTTTATCGACCATGTGCTAAAGATTGACGACCCCGTAGGCGCATCGTCCGTACACGGCGTTTGCG
GTTGCCTGGGAACGATTCTTACAGGACTCTTCGCTACAGAAAAGGGACTGTTCTATGGCGGCGGCAGCAGCTTCCTGCTG
GCACAGCTCTTCGGCGCGGCCGTGGTAGGAGCGTGGGCGGCAGGCATGGGCTTCATTGTCTTTAAAGTGCTGGACAAGAT
ACATGGCTTGCGTGTTCCGAAACGCATTGAAGAGGAAGGACTCGATATCTACGAGCATGGCGAGTCAGCATACAATTAA

Upstream 100 bases:

>100_bases
GTATAATGCAGAACAGGAAAAATAAGCGCAAGCTACTTCCCTTTACCGGCAAAAGTAAAGTCGGGAATAAAACAATACTA
CAACGTAAACGAGAAAAATT

Downstream 100 bases:

>100_bases
TCACAAAAGTTTTTTCCATTCACTCTACTAATCAACAACTAAACAAATTTAGGTATTATGTCAAAATTAAGATTCAGAGT
AGTCGAAACGGCTTTCAAGA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MDTILLFMLPAGLWAQDAGVAATTAAPDATAGALGELATGLNTVWMLLAAMLVFFMQPGFALVEAGFIRTKNTANVLMKN
LVDFMFGSILFWFIGFGLMFGIGGFVGAPHFFNLEAMDKIIDNGLPIEGFLIFQTVFCATAATIVSGAMAERTKFSMYLV
YTVFISVLIYPVSGHWTWGGGWLMNGDEGSFMMRTFGATFHDFAGSTVVHSVGGWIALVGAAILGPRIGKYGKDGKSRAI
PGHSLTLACLGVFILWFGWFGFNPGSQLAAATSGDQTAISHVFLTTNLAACAGGFFALVASWMKYGKPSLSLTLNGVLAG
LVGITAGCDLVSPFGSVLIGAICGVVMIFAVDFIDHVLKIDDPVGASSVHGVCGCLGTILTGLFATEKGLFYGGGSSFLL
AQLFGAAVVGAWAAGMGFIVFKVLDKIHGLRVPKRIEEEGLDIYEHGESAYN

Sequences:

>Translated_452_residues
MDTILLFMLPAGLWAQDAGVAATTAAPDATAGALGELATGLNTVWMLLAAMLVFFMQPGFALVEAGFIRTKNTANVLMKN
LVDFMFGSILFWFIGFGLMFGIGGFVGAPHFFNLEAMDKIIDNGLPIEGFLIFQTVFCATAATIVSGAMAERTKFSMYLV
YTVFISVLIYPVSGHWTWGGGWLMNGDEGSFMMRTFGATFHDFAGSTVVHSVGGWIALVGAAILGPRIGKYGKDGKSRAI
PGHSLTLACLGVFILWFGWFGFNPGSQLAAATSGDQTAISHVFLTTNLAACAGGFFALVASWMKYGKPSLSLTLNGVLAG
LVGITAGCDLVSPFGSVLIGAICGVVMIFAVDFIDHVLKIDDPVGASSVHGVCGCLGTILTGLFATEKGLFYGGGSSFLL
AQLFGAAVVGAWAAGMGFIVFKVLDKIHGLRVPKRIEEEGLDIYEHGESAYN
>Mature_452_residues
MDTILLFMLPAGLWAQDAGVAATTAAPDATAGALGELATGLNTVWMLLAAMLVFFMQPGFALVEAGFIRTKNTANVLMKN
LVDFMFGSILFWFIGFGLMFGIGGFVGAPHFFNLEAMDKIIDNGLPIEGFLIFQTVFCATAATIVSGAMAERTKFSMYLV
YTVFISVLIYPVSGHWTWGGGWLMNGDEGSFMMRTFGATFHDFAGSTVVHSVGGWIALVGAAILGPRIGKYGKDGKSRAI
PGHSLTLACLGVFILWFGWFGFNPGSQLAAATSGDQTAISHVFLTTNLAACAGGFFALVASWMKYGKPSLSLTLNGVLAG
LVGITAGCDLVSPFGSVLIGAICGVVMIFAVDFIDHVLKIDDPVGASSVHGVCGCLGTILTGLFATEKGLFYGGGSSFLL
AQLFGAAVVGAWAAGMGFIVFKVLDKIHGLRVPKRIEEEGLDIYEHGESAYN

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Homo sapiens, GI156627565, Length=181, Percent_Identity=30.3867403314917, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI7706683, Length=174, Percent_Identity=27.5862068965517, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1786656, Length=418, Percent_Identity=35.6459330143541, Blast_Score=208, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI17550180, Length=457, Percent_Identity=35.8862144420131, Blast_Score=228, Evalue=5e-60,
Organism=Caenorhabditis elegans, GI17550182, Length=443, Percent_Identity=35.665914221219, Blast_Score=224, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI133931100, Length=438, Percent_Identity=34.2465753424658, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI32564042, Length=432, Percent_Identity=32.8703703703704, Blast_Score=202, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6324187, Length=448, Percent_Identity=29.6875, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6325396, Length=427, Percent_Identity=29.2740046838407, Blast_Score=129, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6321559, Length=438, Percent_Identity=27.3972602739726, Blast_Score=126, Evalue=6e-30,
Organism=Drosophila melanogaster, GI161078314, Length=422, Percent_Identity=35.781990521327, Blast_Score=214, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 47591; Mature: 47591

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTILLFMLPAGLWAQDAGVAATTAAPDATAGALGELATGLNTVWMLLAAMLVFFMQPGF
CCEEEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ALVEAGFIRTKNTANVLMKNLVDFMFGSILFWFIGFGLMFGIGGFVGAPHFFNLEAMDKI
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHH
IDNGLPIEGFLIFQTVFCATAATIVSGAMAERTKFSMYLVYTVFISVLIYPVSGHWTWGG
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCC
GWLMNGDEGSFMMRTFGATFHDFAGSTVVHSVGGWIALVGAAILGPRIGKYGKDGKSRAI
CEEEECCCCCHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PGHSLTLACLGVFILWFGWFGFNPGSQLAAATSGDQTAISHVFLTTNLAACAGGFFALVA
CCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SWMKYGKPSLSLTLNGVLAGLVGITAGCDLVSPFGSVLIGAICGVVMIFAVDFIDHVLKI
HHHHHCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DDPVGASSVHGVCGCLGTILTGLFATEKGLFYGGGSSFLLAQLFGAAVVGAWAAGMGFIV
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
FKVLDKIHGLRVPKRIEEEGLDIYEHGESAYN
HHHHHHHHCCCCCHHHHHCCCCHHHCCCCCCC
>Mature Secondary Structure
MDTILLFMLPAGLWAQDAGVAATTAAPDATAGALGELATGLNTVWMLLAAMLVFFMQPGF
CCEEEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ALVEAGFIRTKNTANVLMKNLVDFMFGSILFWFIGFGLMFGIGGFVGAPHFFNLEAMDKI
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHHHH
IDNGLPIEGFLIFQTVFCATAATIVSGAMAERTKFSMYLVYTVFISVLIYPVSGHWTWGG
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCC
GWLMNGDEGSFMMRTFGATFHDFAGSTVVHSVGGWIALVGAAILGPRIGKYGKDGKSRAI
CEEEECCCCCHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
PGHSLTLACLGVFILWFGWFGFNPGSQLAAATSGDQTAISHVFLTTNLAACAGGFFALVA
CCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SWMKYGKPSLSLTLNGVLAGLVGITAGCDLVSPFGSVLIGAICGVVMIFAVDFIDHVLKI
HHHHHCCCCEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DDPVGASSVHGVCGCLGTILTGLFATEKGLFYGGGSSFLLAQLFGAAVVGAWAAGMGFIV
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
FKVLDKIHGLRVPKRIEEEGLDIYEHGESAYN
HHHHHHHHCCCCCHHHHHCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]