Click here to switch to the map view.

The map label for this gene is wcaJ [H]

Identifier: 150006429

GI number: 150006429

Start: 4862612

End: 4864069

Strand: Direct

Name: wcaJ [H]

Synonym: BVU_3948

Alternate gene names: 150006429

Gene position: 4862612-4864069 (Clockwise)

Preceding gene: 150006425

Following gene: 150006430

Centisome position: 94.18

GC content: 42.59

Gene sequence:

>1458_bases
TTGCGTTTTAATTTATATGTCCTATATTTGCATACGACTAACATTTTTTTTGTCATGAAACAACCGACAACTGAAAACTC
TGATATAATTAAACTAGTAGCGATATTAGGTGATTTTGCCTTGTTGAACCTTTCTATGATACTTGTGTTTTTTATTCTGA
AAGGAATAGATTCACAGGCCATAGCCCATATTTCATTGAAAACCTACCTGCTGACTTCCAACCTGTGCTATATTCCCTGC
ATATCCATCTTTGGGGTCATACTGCACAACCGCATAGTGCGTCCGGAACAGATTGTAGGCCGCCTGTTAGGAACAATCAG
CTTGCATGTAGTCATCTTCCTCACGGTGCTGGCCATTATTAAGGTAGATAACTTCTCACGCATCTATCTGCTTGCTTTCT
ATCTGTCTTTCATACCTTTAGCTATAGGATGGCGACTGACATTGCGGTTCTTTGTGAAAATGTTCCGCCGCAGTGGCAGG
AACCTGCACACCACTGTACTGATAGGCGACGGGGACAATATGGTGGAGCTGTACCATACGTTGAACGATTTGACTTACGG
ATACAGGGTTTTAGGCATTTTCTATGATGAGAAGGACTCTAATTACCCCGAAGGGATTCCCTTTAAAGGTCCTGTAAACC
AATTATTTGAATGGTTATCCCATAACACCGTGCACGAACTGTACTGTGGTCTGCCATCCAGCAGAAAGGATGATATTCTG
GCCATCATGAACTATTGCGAGAACAATCTGATCCGCTTCTATAGCGTACCTCATGTACGTAATTACATAAAGCGTCAGCT
GCAACTGGAATTATTGGGTGAAGTTCCTGTATTGTCCATCCGTACCGAGCCGTTGCAGAACCCGGTAAACAGACTGGCAA
AACGGTTATTTGATTTGACATTTTCCTCTCTGTTTTTACTTACAATTTTTCCATTTATGTACCTGATTGTAGGTCTTATT
ATTAAAATTACCTCACCTGGTCCCATATTCTTCAAACAGGAGAGAAACGGGGAAAACGGGAAAATCTTTAAATGCTATAA
GTTCCGTTCCATGAAAGTGAATGCATTGTCCGATTCATTGCAGGCTACCAAGAATGATCCCCGCAAGACCAAGTTCGGCA
ACTTCCTGCGTAAAAGCAATCTGGACGAACTGCCGCAATTCATTAATGTATTCAAAGGAGAGATGTCCATTGTAGGTCCC
AGACCCCATATGCTGAAACATACAGAGGAATATTCCCAACTGATTAACAAATACATGATGCGCCACCTTGTCAAACCCGG
AATTACCGGATGGGCGCAAGTCACCGGCTACCGGGGAGAAACCAAAGAACTTTCCCAAATGGAAGGCAGGGTAAGAAGAG
ACTTGTGGTATATTGAGAACTGGACTTTCCTGCTCGACATCCGCATCATGATAAAAACAGTAACCAACATGTTCCGGGGC
GAGAAAAACGCCTATTAA

Upstream 100 bases:

>100_bases
CCTCCGGCCATTATAACGAGGTGATTATTCTTGATGTTCATATGATTCATTTTTTACGTTGTATGACCGCAAAGATAAGT
ATTATTTTTTAAAAAAAGTA

Downstream 100 bases:

>100_bases
TCCAAATCGTAGGGACATATAAAAAATAAAAAGATATGAAAGGTATTGTACTAGCCGGAGGTTCGGGCACGCGCCTCTAT
CCTATTACCAAAGGAGTATC

Product: glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MRFNLYVLYLHTTNIFFVMKQPTTENSDIIKLVAILGDFALLNLSMILVFFILKGIDSQAIAHISLKTYLLTSNLCYIPC
ISIFGVILHNRIVRPEQIVGRLLGTISLHVVIFLTVLAIIKVDNFSRIYLLAFYLSFIPLAIGWRLTLRFFVKMFRRSGR
NLHTTVLIGDGDNMVELYHTLNDLTYGYRVLGIFYDEKDSNYPEGIPFKGPVNQLFEWLSHNTVHELYCGLPSSRKDDIL
AIMNYCENNLIRFYSVPHVRNYIKRQLQLELLGEVPVLSIRTEPLQNPVNRLAKRLFDLTFSSLFLLTIFPFMYLIVGLI
IKITSPGPIFFKQERNGENGKIFKCYKFRSMKVNALSDSLQATKNDPRKTKFGNFLRKSNLDELPQFINVFKGEMSIVGP
RPHMLKHTEEYSQLINKYMMRHLVKPGITGWAQVTGYRGETKELSQMEGRVRRDLWYIENWTFLLDIRIMIKTVTNMFRG
EKNAY

Sequences:

>Translated_485_residues
MRFNLYVLYLHTTNIFFVMKQPTTENSDIIKLVAILGDFALLNLSMILVFFILKGIDSQAIAHISLKTYLLTSNLCYIPC
ISIFGVILHNRIVRPEQIVGRLLGTISLHVVIFLTVLAIIKVDNFSRIYLLAFYLSFIPLAIGWRLTLRFFVKMFRRSGR
NLHTTVLIGDGDNMVELYHTLNDLTYGYRVLGIFYDEKDSNYPEGIPFKGPVNQLFEWLSHNTVHELYCGLPSSRKDDIL
AIMNYCENNLIRFYSVPHVRNYIKRQLQLELLGEVPVLSIRTEPLQNPVNRLAKRLFDLTFSSLFLLTIFPFMYLIVGLI
IKITSPGPIFFKQERNGENGKIFKCYKFRSMKVNALSDSLQATKNDPRKTKFGNFLRKSNLDELPQFINVFKGEMSIVGP
RPHMLKHTEEYSQLINKYMMRHLVKPGITGWAQVTGYRGETKELSQMEGRVRRDLWYIENWTFLLDIRIMIKTVTNMFRG
EKNAY
>Mature_485_residues
MRFNLYVLYLHTTNIFFVMKQPTTENSDIIKLVAILGDFALLNLSMILVFFILKGIDSQAIAHISLKTYLLTSNLCYIPC
ISIFGVILHNRIVRPEQIVGRLLGTISLHVVIFLTVLAIIKVDNFSRIYLLAFYLSFIPLAIGWRLTLRFFVKMFRRSGR
NLHTTVLIGDGDNMVELYHTLNDLTYGYRVLGIFYDEKDSNYPEGIPFKGPVNQLFEWLSHNTVHELYCGLPSSRKDDIL
AIMNYCENNLIRFYSVPHVRNYIKRQLQLELLGEVPVLSIRTEPLQNPVNRLAKRLFDLTFSSLFLLTIFPFMYLIVGLI
IKITSPGPIFFKQERNGENGKIFKCYKFRSMKVNALSDSLQATKNDPRKTKFGNFLRKSNLDELPQFINVFKGEMSIVGP
RPHMLKHTEEYSQLINKYMMRHLVKPGITGWAQVTGYRGETKELSQMEGRVRRDLWYIENWTFLLDIRIMIKTVTNMFRG
EKNAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=372, Percent_Identity=40.5913978494624, Blast_Score=252, Evalue=4e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 56452; Mature: 56452

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFNLYVLYLHTTNIFFVMKQPTTENSDIIKLVAILGDFALLNLSMILVFFILKGIDSQA
CEEEEEEEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
IAHISLKTYLLTSNLCYIPCISIFGVILHNRIVRPEQIVGRLLGTISLHVVIFLTVLAII
EEEHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KVDNFSRIYLLAFYLSFIPLAIGWRLTLRFFVKMFRRSGRNLHTTVLIGDGDNMVELYHT
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHH
LNDLTYGYRVLGIFYDEKDSNYPEGIPFKGPVNQLFEWLSHNTVHELYCGLPSSRKDDIL
HHHHHHCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHCCCCCCCCCHHH
AIMNYCENNLIRFYSVPHVRNYIKRQLQLELLGEVPVLSIRTEPLQNPVNRLAKRLFDLT
HHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHH
FSSLFLLTIFPFMYLIVGLIIKITSPGPIFFKQERNGENGKIFKCYKFRSMKVNALSDSL
HHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCCCEEEEEEECCEEEHHHHHHH
QATKNDPRKTKFGNFLRKSNLDELPQFINVFKGEMSIVGPRPHMLKHTEEYSQLINKYMM
HHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHH
RHLVKPGITGWAQVTGYRGETKELSQMEGRVRRDLWYIENWTFLLDIRIMIKTVTNMFRG
HHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHEEEECEEEEHHHHHHHHHHHHHHCC
EKNAY
CCCCC
>Mature Secondary Structure
MRFNLYVLYLHTTNIFFVMKQPTTENSDIIKLVAILGDFALLNLSMILVFFILKGIDSQA
CEEEEEEEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
IAHISLKTYLLTSNLCYIPCISIFGVILHNRIVRPEQIVGRLLGTISLHVVIFLTVLAII
EEEHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KVDNFSRIYLLAFYLSFIPLAIGWRLTLRFFVKMFRRSGRNLHTTVLIGDGDNMVELYHT
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHH
LNDLTYGYRVLGIFYDEKDSNYPEGIPFKGPVNQLFEWLSHNTVHELYCGLPSSRKDDIL
HHHHHHCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHCCCCCCCCCHHH
AIMNYCENNLIRFYSVPHVRNYIKRQLQLELLGEVPVLSIRTEPLQNPVNRLAKRLFDLT
HHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHH
FSSLFLLTIFPFMYLIVGLIIKITSPGPIFFKQERNGENGKIFKCYKFRSMKVNALSDSL
HHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCCCEEEEEEECCEEEHHHHHHH
QATKNDPRKTKFGNFLRKSNLDELPQFINVFKGEMSIVGPRPHMLKHTEEYSQLINKYMM
HHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHH
RHLVKPGITGWAQVTGYRGETKELSQMEGRVRRDLWYIENWTFLLDIRIMIKTVTNMFRG
HHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHEEEECEEEEHHHHHHHHHHHHHHCC
EKNAY
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]