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The map label for this gene is gadB [H]

Identifier: 150006376

GI number: 150006376

Start: 4800528

End: 4801976

Strand: Direct

Name: gadB [H]

Synonym: BVU_3895

Alternate gene names: 150006376

Gene position: 4800528-4801976 (Clockwise)

Preceding gene: 150006360

Following gene: 150006377

Centisome position: 92.98

GC content: 47.76

Gene sequence:

>1449_bases
ATGAAAGAATGTAATTGTGATTTGAGAGACGGTGACGCAAAGACAGCCGTGTTTGGTTCAAACGAAATGCTACAGCCTGC
CCCGGTAGATACCATCCCTATGGAACCTACCACCCCACAGATTGCTTATCAAATGGTAAAAGACGAAACATTCGCCCAAA
CCCAGCCACGCCTTAACCTGGCCACTTTCGTTACCACCTACATGGACGACTATGCCACCAAACTGATGAACGAAGCCATC
AGCATCAACTACATTGATGAAACAGAATATCCTCGTATCGCAGTGATGAATGCCAAATGTATCAATATCATGGCTAATTT
GTGGAATTCGCCCGAACAGGCAAAATGGAAAACCGGAGCACTGGCTATCGGCTCCAGCGAGGCTTGTATGCTGGGAGGAG
TAGCCGCCTGGTTACGCTGGAAAGACAAACGTAAAGCTCAGGGGAAACCGACTGACAAGCCTAACTTTGTCATTTCAAGC
GGTTTTCAGGTTGTTTGGGAAAAGTTCGCCCAACTTTGGCAAATTGAAATGCGCCAAGTACCTCTCACCTTGGACAAAAC
CACTCTGGACCCCGAAGAAGCACTGAAGATGTGTGACGAGAACACCATCTGCATCGTCCCCATCCAAGGTGTGACATGGA
CAGGTTTGAACGATGACGTGGAAGCGTTGGACAAAGCACTGGATGCCTACAACGCCAAAACCGGCTATGATATTCCTATC
CATGTAGATGCTGCAACCGGCGGTTTCATCCTGCCGTTCCTCAATCCCGACACAAAATGGGACTTCCGCTTGAAATGGGT
TCTCTCCATCAGCACTTCCGGCCACAAGTTCGGACTGGTTTATCCGGGACTGGGATGGGTAGTATGGAAAGACAAGAAAT
ACCTTCCGGATGCCATGTCGTTCAGTGTCAATTATCTGGGTGCGAACATCACACAGGTTGGTTTGAACTTCAGCCGTCCC
GCCGCACAGATTCTGGGACAGTACTACCAATTTATCCGTCTGGGATTCCAAGGATACAAAGCCATCCAATACAATTCCAT
GGAAATCACCAAGTACATCCACAGCGAAATAGCCAAGATGGCTCCGTTCGTAAATTACAGCGATGACGTAGTAAATCCTT
TGTTCATCTGGTACATGAAACCCGAATATGCAAAGAATGCCAAATGGACTCTGTACGATTTGCAGGCTAAATTGCAGCAA
AGCGGCTGGATGGTTCCTGCCTACACCTTGCCGGAAAATATCCAGAATTACGTAGTTATGCGTGTTGTGGTACGTCAGGG
ATTCAGCCGTGACATGGCGGATATGTTGTTGAACGACATCAAGAATGCCATCTGCGAGTTCGAGAAACTGGAATATCCCA
CTCCTACCCGCATCGCCCAAGACAAGAATATCGCTGTAAAAGGTACTGTGTTCACTCACAATGCCCATAGCAACGCCGCT
AAAAAATAA

Upstream 100 bases:

>100_bases
ATAAAATCAAGAAACTTTTCTCCAAACCTCCTGTTGAGGGAGAAAAACGCGGGAAACTCCCATGTTTTCCATTGTATTAC
TAATCTAAACTTTAAGTAAA

Downstream 100 bases:

>100_bases
GCATAGATAACAATAATGCAACCATCCGCCGTTAAGGTAGATGGTTACTTCAATCAAATTAATTTATGGAAAGAACTATA
ACTATCCAGCAAATAAAGGA

Product: glutamate decarboxylase

Products: NA

Alternate protein names: GAD-beta [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MKECNCDLRDGDAKTAVFGSNEMLQPAPVDTIPMEPTTPQIAYQMVKDETFAQTQPRLNLATFVTTYMDDYATKLMNEAI
SINYIDETEYPRIAVMNAKCINIMANLWNSPEQAKWKTGALAIGSSEACMLGGVAAWLRWKDKRKAQGKPTDKPNFVISS
GFQVVWEKFAQLWQIEMRQVPLTLDKTTLDPEEALKMCDENTICIVPIQGVTWTGLNDDVEALDKALDAYNAKTGYDIPI
HVDAATGGFILPFLNPDTKWDFRLKWVLSISTSGHKFGLVYPGLGWVVWKDKKYLPDAMSFSVNYLGANITQVGLNFSRP
AAQILGQYYQFIRLGFQGYKAIQYNSMEITKYIHSEIAKMAPFVNYSDDVVNPLFIWYMKPEYAKNAKWTLYDLQAKLQQ
SGWMVPAYTLPENIQNYVVMRVVVRQGFSRDMADMLLNDIKNAICEFEKLEYPTPTRIAQDKNIAVKGTVFTHNAHSNAA
KK

Sequences:

>Translated_482_residues
MKECNCDLRDGDAKTAVFGSNEMLQPAPVDTIPMEPTTPQIAYQMVKDETFAQTQPRLNLATFVTTYMDDYATKLMNEAI
SINYIDETEYPRIAVMNAKCINIMANLWNSPEQAKWKTGALAIGSSEACMLGGVAAWLRWKDKRKAQGKPTDKPNFVISS
GFQVVWEKFAQLWQIEMRQVPLTLDKTTLDPEEALKMCDENTICIVPIQGVTWTGLNDDVEALDKALDAYNAKTGYDIPI
HVDAATGGFILPFLNPDTKWDFRLKWVLSISTSGHKFGLVYPGLGWVVWKDKKYLPDAMSFSVNYLGANITQVGLNFSRP
AAQILGQYYQFIRLGFQGYKAIQYNSMEITKYIHSEIAKMAPFVNYSDDVVNPLFIWYMKPEYAKNAKWTLYDLQAKLQQ
SGWMVPAYTLPENIQNYVVMRVVVRQGFSRDMADMLLNDIKNAICEFEKLEYPTPTRIAQDKNIAVKGTVFTHNAHSNAA
KK
>Mature_482_residues
MKECNCDLRDGDAKTAVFGSNEMLQPAPVDTIPMEPTTPQIAYQMVKDETFAQTQPRLNLATFVTTYMDDYATKLMNEAI
SINYIDETEYPRIAVMNAKCINIMANLWNSPEQAKWKTGALAIGSSEACMLGGVAAWLRWKDKRKAQGKPTDKPNFVISS
GFQVVWEKFAQLWQIEMRQVPLTLDKTTLDPEEALKMCDENTICIVPIQGVTWTGLNDDVEALDKALDAYNAKTGYDIPI
HVDAATGGFILPFLNPDTKWDFRLKWVLSISTSGHKFGLVYPGLGWVVWKDKKYLPDAMSFSVNYLGANITQVGLNFSRP
AAQILGQYYQFIRLGFQGYKAIQYNSMEITKYIHSEIAKMAPFVNYSDDVVNPLFIWYMKPEYAKNAKWTLYDLQAKLQQ
SGWMVPAYTLPENIQNYVVMRVVVRQGFSRDMADMLLNDIKNAICEFEKLEYPTPTRIAQDKNIAVKGTVFTHNAHSNAA
KK

Specific function: Converts internalized glutamate to GABA and increases the internal pH. Involved in glutamate-dependent acid resistance in gastric fluid [H]

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=369, Percent_Identity=23.0352303523035, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1787769, Length=447, Percent_Identity=42.2818791946309, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI1789934, Length=447, Percent_Identity=42.2818791946309, Blast_Score=381, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17557272, Length=369, Percent_Identity=22.7642276422764, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17543922, Length=278, Percent_Identity=24.8201438848921, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25148342, Length=367, Percent_Identity=20.708446866485, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6323905, Length=492, Percent_Identity=33.5365853658537, Blast_Score=269, Evalue=6e-73,
Organism=Saccharomyces cerevisiae, GI6320500, Length=402, Percent_Identity=20.3980099502488, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI21355963, Length=388, Percent_Identity=23.9690721649485, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24654344, Length=388, Percent_Identity=23.9690721649485, Blast_Score=99, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010107
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.1.15 [H]

Molecular weight: Translated: 54642; Mature: 54642

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKECNCDLRDGDAKTAVFGSNEMLQPAPVDTIPMEPTTPQIAYQMVKDETFAQTQPRLNL
CCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHCCCCCHH
ATFVTTYMDDYATKLMNEAISINYIDETEYPRIAVMNAKCINIMANLWNSPEQAKWKTGA
HHHHHHHHHHHHHHHHHHCEEEEEECCCCCCEEEEECHHHHHHHHHHCCCCCCCCCCCCE
LAIGSSEACMLGGVAAWLRWKDKRKAQGKPTDKPNFVISSGFQVVWEKFAQLWQIEMRQV
EEECCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHC
PLTLDKTTLDPEEALKMCDENTICIVPIQGVTWTGLNDDVEALDKALDAYNAKTGYDIPI
CEEECCCCCCHHHHHHHCCCCCEEEEEECCEEECCCCCHHHHHHHHHHHCCCCCCCCEEE
HVDAATGGFILPFLNPDTKWDFRLKWVLSISTSGHKFGLVYPGLGWVVWKDKKYLPDAMS
EEECCCCCEEEEECCCCCCEEEEEEEEEEEECCCCEEEEEECCCEEEEECCCCCCCCHHH
FSVNYLGANITQVGLNFSRPAAQILGQYYQFIRLGFQGYKAIQYNSMEITKYIHSEIAKM
EEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHH
APFVNYSDDVVNPLFIWYMKPEYAKNAKWTLYDLQAKLQQSGWMVPAYTLPENIQNYVVM
CCCCCCCCCCCCEEEEEEECHHHCCCCCEEEEHHHHHHHHCCCEEEEEECCHHHHHHHHH
RVVVRQGFSRDMADMLLNDIKNAICEFEKLEYPTPTRIAQDKNIAVKGTVFTHNAHSNAA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCCCC
KK
CC
>Mature Secondary Structure
MKECNCDLRDGDAKTAVFGSNEMLQPAPVDTIPMEPTTPQIAYQMVKDETFAQTQPRLNL
CCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHCCCCCHH
ATFVTTYMDDYATKLMNEAISINYIDETEYPRIAVMNAKCINIMANLWNSPEQAKWKTGA
HHHHHHHHHHHHHHHHHHCEEEEEECCCCCCEEEEECHHHHHHHHHHCCCCCCCCCCCCE
LAIGSSEACMLGGVAAWLRWKDKRKAQGKPTDKPNFVISSGFQVVWEKFAQLWQIEMRQV
EEECCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHC
PLTLDKTTLDPEEALKMCDENTICIVPIQGVTWTGLNDDVEALDKALDAYNAKTGYDIPI
CEEECCCCCCHHHHHHHCCCCCEEEEEECCEEECCCCCHHHHHHHHHHHCCCCCCCCEEE
HVDAATGGFILPFLNPDTKWDFRLKWVLSISTSGHKFGLVYPGLGWVVWKDKKYLPDAMS
EEECCCCCEEEEECCCCCCEEEEEEEEEEEECCCCEEEEEECCCEEEEECCCCCCCCHHH
FSVNYLGANITQVGLNFSRPAAQILGQYYQFIRLGFQGYKAIQYNSMEITKYIHSEIAKM
EEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHH
APFVNYSDDVVNPLFIWYMKPEYAKNAKWTLYDLQAKLQQSGWMVPAYTLPENIQNYVVM
CCCCCCCCCCCCEEEEEEECHHHCCCCCEEEEHHHHHHHHCCCEEEEEECCHHHHHHHHH
RVVVRQGFSRDMADMLLNDIKNAICEFEKLEYPTPTRIAQDKNIAVKGTVFTHNAHSNAA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEECCCCCCC
KK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]