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The map label for this gene is yeiE [H]

Identifier: 150006302

GI number: 150006302

Start: 4711946

End: 4712842

Strand: Reverse

Name: yeiE [H]

Synonym: BVU_3813

Alternate gene names: 150006302

Gene position: 4712842-4711946 (Counterclockwise)

Preceding gene: 150006303

Following gene: 150006301

Centisome position: 91.28

GC content: 42.03

Gene sequence:

>897_bases
ATGTCTGATTTCAGGTTAAGAGTATTCAGTAGTGTTGCGAAAAACCTCAGCTTTACAAAGGCTTCGCAAGAGCTGTTTAT
TAGTCAGCCGGCTATAACCAAGCATATTCAGGAATTGGAGACGATGTACCAGACACGCCTTTTTGAGCGAATGGGGAACA
AGATCTTGCTGACTGATGCAGGCAGGTTGTTGTTGGAGCATTGTGAGAAAATTTTGGAAGATTACGGACGCTTGGAGTAC
GAGATGAACTTATTACGTAATGAGCATACCGGTGAGTTGCGCTTGGGAGCCAGTACTACAATTGCCCAATATGTGCTTCC
CCCTTTGCTGGCCCGCTTCATCGAGAAGTTTCCGCAAGTGTCTTTATCACTTTTCAGCGGGAACTCCTCTGAGGTGGAAA
AGGCATTGCAGGAACATCGTATAGATTTGGCACTGGTAGAGGGAAATACCCGGCAACCTAATTTGAAATATACTCCTTTT
CTGCAAGATGAGCTGGTGGCTATCGTCCATACTCATAGTAAGTGGATGGATTATGATGAGATAACTTTGGATGAATTGAA
AGAAGTTCCTCTTGTACTTCGTGAAAGAGGTTCGGGAACATTGGATGTATTGATTACCGCTTTGCATAAACACAACATAA
AGTTATCGGACCTTACTATCCGTATGCACCTGGGAAGTACAGAGAGCATTAAATTATTTCTGGAAAATAGTGAGTGCATG
GGGATTTTGTCTATCCGGTCTATTAATAAAGAACTGTATTCAGGGAAGTTTAAAGTATTGGATATAAAAGACCTGGTGAT
GTTTCGTGAATTTGATTTTGTCCAATTGCAAGGGCAGGATACAGGGCTCCCGGCTCTGTTCATGCAATTTGCCAATCATT
ATAAGGAAAAGTTATAA

Upstream 100 bases:

>100_bases
TCCTCCAACCAGTGAAGTCGCTTATCAGTGGGAGATGTTTGGATTCGCTGTTTTACAGTCGTTAGGAATTAAAGCAGACG
AACTATAATCATTAAAAAAC

Downstream 100 bases:

>100_bases
CTTATAATTAAAAACGATTAGATTGTTTTTGAAACATATATTACCTTTGCATCCCGAAATGGAGAAATAATGTTAAAAAG
TAAAATATATGATTTCAGTA

Product: putative transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSDFRLRVFSSVAKNLSFTKASQELFISQPAITKHIQELETMYQTRLFERMGNKILLTDAGRLLLEHCEKILEDYGRLEY
EMNLLRNEHTGELRLGASTTIAQYVLPPLLARFIEKFPQVSLSLFSGNSSEVEKALQEHRIDLALVEGNTRQPNLKYTPF
LQDELVAIVHTHSKWMDYDEITLDELKEVPLVLRERGSGTLDVLITALHKHNIKLSDLTIRMHLGSTESIKLFLENSECM
GILSIRSINKELYSGKFKVLDIKDLVMFREFDFVQLQGQDTGLPALFMQFANHYKEKL

Sequences:

>Translated_298_residues
MSDFRLRVFSSVAKNLSFTKASQELFISQPAITKHIQELETMYQTRLFERMGNKILLTDAGRLLLEHCEKILEDYGRLEY
EMNLLRNEHTGELRLGASTTIAQYVLPPLLARFIEKFPQVSLSLFSGNSSEVEKALQEHRIDLALVEGNTRQPNLKYTPF
LQDELVAIVHTHSKWMDYDEITLDELKEVPLVLRERGSGTLDVLITALHKHNIKLSDLTIRMHLGSTESIKLFLENSECM
GILSIRSINKELYSGKFKVLDIKDLVMFREFDFVQLQGQDTGLPALFMQFANHYKEKL
>Mature_297_residues
SDFRLRVFSSVAKNLSFTKASQELFISQPAITKHIQELETMYQTRLFERMGNKILLTDAGRLLLEHCEKILEDYGRLEYE
MNLLRNEHTGELRLGASTTIAQYVLPPLLARFIEKFPQVSLSLFSGNSSEVEKALQEHRIDLALVEGNTRQPNLKYTPFL
QDELVAIVHTHSKWMDYDEITLDELKEVPLVLRERGSGTLDVLITALHKHNIKLSDLTIRMHLGSTESIKLFLENSECMG
ILSIRSINKELYSGKFKVLDIKDLVMFREFDFVQLQGQDTGLPALFMQFANHYKEKL

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=254, Percent_Identity=33.4645669291339, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI157672245, Length=185, Percent_Identity=29.1891891891892, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788748, Length=217, Percent_Identity=25.3456221198157, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1786401, Length=295, Percent_Identity=25.7627118644068, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789506, Length=215, Percent_Identity=26.5116279069767, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1788296, Length=196, Percent_Identity=27.5510204081633, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1789639, Length=123, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787601, Length=141, Percent_Identity=29.7872340425532, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787879, Length=197, Percent_Identity=28.9340101522843, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87082132, Length=147, Percent_Identity=34.6938775510204, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787530, Length=246, Percent_Identity=24.7967479674797, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI145693105, Length=161, Percent_Identity=24.223602484472, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1786448, Length=165, Percent_Identity=30.3030303030303, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI2367136, Length=150, Percent_Identity=30.6666666666667, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787128, Length=129, Percent_Identity=34.8837209302326, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787949, Length=269, Percent_Identity=24.5353159851301, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788887, Length=203, Percent_Identity=26.6009852216749, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789204, Length=142, Percent_Identity=29.5774647887324, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788626, Length=217, Percent_Identity=29.0322580645161, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790399, Length=192, Percent_Identity=20.8333333333333, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI87081904, Length=200, Percent_Identity=26.5, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1788297, Length=162, Percent_Identity=24.6913580246914, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1789173, Length=164, Percent_Identity=26.8292682926829, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788706, Length=161, Percent_Identity=31.055900621118, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34366; Mature: 34235

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFRLRVFSSVAKNLSFTKASQELFISQPAITKHIQELETMYQTRLFERMGNKILLTDA
CCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEHH
GRLLLEHCEKILEDYGRLEYEMNLLRNEHTGELRLGASTTIAQYVLPPLLARFIEKFPQV
HHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCC
SLSLFSGNSSEVEKALQEHRIDLALVEGNTRQPNLKYTPFLQDELVAIVHTHSKWMDYDE
EEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHEEEHHHHHCCCCC
ITLDELKEVPLVLRERGSGTLDVLITALHKHNIKLSDLTIRMHLGSTESIKLFLENSECM
CCHHHHHHCCHHHHHCCCCHHHHHHHHHHHCCCEEEEEEEEEECCCCCEEEEEEECCCCE
GILSIRSINKELYSGKFKVLDIKDLVMFREFDFVQLQGQDTGLPALFMQFANHYKEKL
EEHHHHHHHHHHHCCCEEEEEHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDFRLRVFSSVAKNLSFTKASQELFISQPAITKHIQELETMYQTRLFERMGNKILLTDA
CCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEHH
GRLLLEHCEKILEDYGRLEYEMNLLRNEHTGELRLGASTTIAQYVLPPLLARFIEKFPQV
HHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCC
SLSLFSGNSSEVEKALQEHRIDLALVEGNTRQPNLKYTPFLQDELVAIVHTHSKWMDYDE
EEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHEEEHHHHHCCCCC
ITLDELKEVPLVLRERGSGTLDVLITALHKHNIKLSDLTIRMHLGSTESIKLFLENSECM
CCHHHHHHCCHHHHHCCCCHHHHHHHHHHHCCCEEEEEEEEEECCCCCEEEEEEECCCCE
GILSIRSINKELYSGKFKVLDIKDLVMFREFDFVQLQGQDTGLPALFMQFANHYKEKL
EEHHHHHHHHHHHCCCEEEEEHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]