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The map label for this gene is ald [H]

Identifier: 150006296

GI number: 150006296

Start: 4705921

End: 4707012

Strand: Direct

Name: ald [H]

Synonym: BVU_3807

Alternate gene names: 150006296

Gene position: 4705921-4707012 (Clockwise)

Preceding gene: 150006291

Following gene: 150006299

Centisome position: 91.14

GC content: 45.79

Gene sequence:

>1092_bases
ATGATAGTAGGAATTCCTAAAGAAATCAAAAACAACGAGAATCGTGTATCATTAACCCCTGCAGGAGCACACGAGTTGGT
ACAGAGAGGTCACACGGTGTATATACAACACACCGCAGGTATAAACAGCGGATTTTCCGATGAAGAGTATGAGAAAGTCG
GAGCGCGTATCCTTCCTACTATAGAAGATGTGTACGCCATTGCCGAGATGATTATAAAAGTCAAGGAACCCATCGAATGT
GAATATAACCTGGTACGTAAGGGCCAACTGGTATTTACATACTTCCATTTTGCCTGTGACAAAGAATTAACGGAGGCAAT
GATGCGTAGTGGTTCAGTATGCCTAGCTTACGAAACTGTAACAGACAAACAAGGAGGACTGCCTCTGCTGATTCCCATGA
GTGAAGTGGCGGGACGAATGTCCACTCAGGAAGGAGCACGTTTTTTGGAGAAGCCTCAAGGAGGACGTGGCATCTTGCTA
GGTGGAGTTCCCGGTGTCAAGCCAGCCAAAGTGCTGGTATTAGGCGGCGGCATTGTAGGAACCAATGCAGCTTTAATGGC
TGCAGGATTAGGGGCTGATGTCACAATCTGTGATATTTCATTACCCCGCCTGCGTCAGTTGAGTGAGTTCATGCCTAAAA
ATGTAAAAACATTATTCTCGTCCAGCCACAATATAGAAAAAGAACTGCCAACAACTGATTTGATAATCGGTGCAGTTCTT
ATCCCCGGTGCAAAGGCTCCCCACCTCATAACACGCCCTATGCTGAAACTGATGAAAAAGGGTACTGTACTGGTAGATGT
GGCCATTGACCAAGGAGGATGTTTTGAAACCTCCCATCCCACAACTCATGCCGAACCCATTTACGAAGTAGACGGTATCA
TCCATTACTGTGTGGCAAATATTCCGGGAGCCGTGCCGTGTACTTCAACCTTGGCATTAACCAATGCTACACTGCCATAC
GCTATCAGATTGGCAGATCTTGGTTGGGAAAAAGCTTGCGAAAACGATCCGGGACTAAAAAACGGCTTGAATATTGTAAA
CGGAAAGATTGTGTTTCCGGCCGTAGCAGAAGCCTTTGGCTGGAAAATTTAA

Upstream 100 bases:

>100_bases
TAAACATACATTTATTACGAAAAAAACATATCCGGACAAAACCTACTTTTCTGACATTTTGTTATCTTTGAGGCAGTCAA
ACCCCTTAAATGTGACAATT

Downstream 100 bases:

>100_bases
TAAAGTATGGATCCTAATGGAAAAAGGAAGGCCTATCATGAAAGGTCTTCCTTTTTTACGGAATAACATTTATAAGGATT
ATCATCTCATGATTTTTCAA

Product: alanine dehydrogenase

Products: NA

Alternate protein names: Stage V sporulation protein N [H]

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MIVGIPKEIKNNENRVSLTPAGAHELVQRGHTVYIQHTAGINSGFSDEEYEKVGARILPTIEDVYAIAEMIIKVKEPIEC
EYNLVRKGQLVFTYFHFACDKELTEAMMRSGSVCLAYETVTDKQGGLPLLIPMSEVAGRMSTQEGARFLEKPQGGRGILL
GGVPGVKPAKVLVLGGGIVGTNAALMAAGLGADVTICDISLPRLRQLSEFMPKNVKTLFSSSHNIEKELPTTDLIIGAVL
IPGAKAPHLITRPMLKLMKKGTVLVDVAIDQGGCFETSHPTTHAEPIYEVDGIIHYCVANIPGAVPCTSTLALTNATLPY
AIRLADLGWEKACENDPGLKNGLNIVNGKIVFPAVAEAFGWKI

Sequences:

>Translated_363_residues
MIVGIPKEIKNNENRVSLTPAGAHELVQRGHTVYIQHTAGINSGFSDEEYEKVGARILPTIEDVYAIAEMIIKVKEPIEC
EYNLVRKGQLVFTYFHFACDKELTEAMMRSGSVCLAYETVTDKQGGLPLLIPMSEVAGRMSTQEGARFLEKPQGGRGILL
GGVPGVKPAKVLVLGGGIVGTNAALMAAGLGADVTICDISLPRLRQLSEFMPKNVKTLFSSSHNIEKELPTTDLIIGAVL
IPGAKAPHLITRPMLKLMKKGTVLVDVAIDQGGCFETSHPTTHAEPIYEVDGIIHYCVANIPGAVPCTSTLALTNATLPY
AIRLADLGWEKACENDPGLKNGLNIVNGKIVFPAVAEAFGWKI
>Mature_363_residues
MIVGIPKEIKNNENRVSLTPAGAHELVQRGHTVYIQHTAGINSGFSDEEYEKVGARILPTIEDVYAIAEMIIKVKEPIEC
EYNLVRKGQLVFTYFHFACDKELTEAMMRSGSVCLAYETVTDKQGGLPLLIPMSEVAGRMSTQEGARFLEKPQGGRGILL
GGVPGVKPAKVLVLGGGIVGTNAALMAAGLGADVTICDISLPRLRQLSEFMPKNVKTLFSSSHNIEKELPTTDLIIGAVL
IPGAKAPHLITRPMLKLMKKGTVLVDVAIDQGGCFETSHPTTHAEPIYEVDGIIHYCVANIPGAVPCTSTLALTNATLPY
AIRLADLGWEKACENDPGLKNGLNIVNGKIVFPAVAEAFGWKI

Specific function: This enzyme is a key factor in the assimilation of L- alanine as an energy source through the tricarboxylic acid cycle during sporulation [H]

COG id: COG0686

COG function: function code E; Alanine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=392, Percent_Identity=29.8469387755102, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI122939153, Length=392, Percent_Identity=29.8469387755102, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1787887, Length=333, Percent_Identity=29.4294294294294, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17550456, Length=370, Percent_Identity=28.6486486486486, Blast_Score=135, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886
- InterPro:   IPR008141 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.4.1.1 [H]

Molecular weight: Translated: 38997; Mature: 38997

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVGIPKEIKNNENRVSLTPAGAHELVQRGHTVYIQHTAGINSGFSDEEYEKVGARILPT
CEECCCHHHCCCCCEEEECCCCHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHCCHHCCH
IEDVYAIAEMIIKVKEPIECEYNLVRKGQLVFTYFHFACDKELTEAMMRSGSVCLAYETV
HHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEHHHHHCHHHHHHHHHCCCEEEEEEEE
TDKQGGLPLLIPMSEVAGRMSTQEGARFLEKPQGGRGILLGGVPGVKPAKVLVLGGGIVG
CCCCCCCEEEEEHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCEEC
TNAALMAAGLGADVTICDISLPRLRQLSEFMPKNVKTLFSSSHNIEKELPTTDLIIGAVL
CCHHHHHHCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHCCCCCCHHHCCHHHHHHHHHC
IPGAKAPHLITRPMLKLMKKGTVLVDVAIDQGGCFETSHPTTHAEPIYEVDGIIHYCVAN
CCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
IPGAVPCTSTLALTNATLPYAIRLADLGWEKACENDPGLKNGLNIVNGKIVFPAVAEAFG
CCCCCCCCCEEEEECCCCCEEEEEECCCHHHHCCCCCCCCCCCEEECCEEEHHHHHHHHC
WKI
CCC
>Mature Secondary Structure
MIVGIPKEIKNNENRVSLTPAGAHELVQRGHTVYIQHTAGINSGFSDEEYEKVGARILPT
CEECCCHHHCCCCCEEEECCCCHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHCCHHCCH
IEDVYAIAEMIIKVKEPIECEYNLVRKGQLVFTYFHFACDKELTEAMMRSGSVCLAYETV
HHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEHHHHHCHHHHHHHHHCCCEEEEEEEE
TDKQGGLPLLIPMSEVAGRMSTQEGARFLEKPQGGRGILLGGVPGVKPAKVLVLGGGIVG
CCCCCCCEEEEEHHHHHCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEECCEEC
TNAALMAAGLGADVTICDISLPRLRQLSEFMPKNVKTLFSSSHNIEKELPTTDLIIGAVL
CCHHHHHHCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHCCCCCCHHHCCHHHHHHHHHC
IPGAKAPHLITRPMLKLMKKGTVLVDVAIDQGGCFETSHPTTHAEPIYEVDGIIHYCVAN
CCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
IPGAVPCTSTLALTNATLPYAIRLADLGWEKACENDPGLKNGLNIVNGKIVFPAVAEAFG
CCCCCCCCCEEEEECCCCCEEEEEECCCHHHHCCCCCCCCCCCEEECCEEEHHHHHHHHC
WKI
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226620; 9384377 [H]